BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5124

Title: Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy   PubMed: 11714920

Authors: Chamberlain, Aaron; Receveur, Veronique; Spencer, Andrew; Redfield, Christina; Dobson, Christopher

Citation: Chamberlain, Aaron; Receveur, Veronique; Spencer, Andrew; Redfield, Christina; Dobson, Christopher. "Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy"  Protein Sci. 10, 2525-2530 (2001).

Assembly members:
lysozyme, polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:   Production method: recombinant technology   Host organism: Aspergillus niger

Entity Sequences (FASTA):
lysozyme: KVFERCELARTLKRLGMDGY RGISLANWMCLAKWESGYNT RATNYNAGDRSTDYGTFQIN SRYWCNDGKTPGAVNACHLS CSALLQDNIADAVACAKRVV RDPQGIRAWVAWRNRCQNRD VRQYVQGCGV

Data sets:
Data typeCount
15N chemical shifts127
1H chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lysozyme I56T1

Entities:

Entity 1, lysozyme I56T 130 residues - Formula weight is not available

1   LYSVALPHEGLUARGCYSGLULEUALAARG
2   THRLEULYSARGLEUGLYMETASPGLYTYR
3   ARGGLYILESERLEUALAASNTRPMETCYS
4   LEUALALYSTRPGLUSERGLYTYRASNTHR
5   ARGALATHRASNTYRASNALAGLYASPARG
6   SERTHRASPTYRGLYTHRPHEGLNILEASN
7   SERARGTYRTRPCYSASNASPGLYLYSTHR
8   PROGLYALAVALASNALACYSHISLEUSER
9   CYSSERALALEULEUGLNASPASNILEALA
10   ASPALAVALALACYSALALYSARGVALVAL
11   ARGASPPROGLNGLYILEARGALATRPVAL
12   ALATRPARGASNARGCYSGLNASNARGASP
13   VALARGGLNTYRVALGLNGLYCYSGLYVAL

Samples:

sample: lysozyme0.8 – 1.2 mM; HCl mM; H2O 100%

condition: pH: 5.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HSQCnot availablenot availablenot available
COSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 2542 5123 5125 5130 5142 76
PDB
DBJ BAA00314 BAG34722 BAG73364
EMBL CAA32175 CAA53144
GB AAA36188 AAA59535 AAA59536 AAA72819 AAB26052
REF NP_000230 NP_001009073 NP_001266591 XP_002823550 XP_003808770
SP P61626 P61627 P61628 P79179 P79239
TPE CDM98740 CDM98750