BMRB Entry 5246

Title:
Chiral Mutagenesis of Insulin's Hidden Receptor-Binding Surface: Structure of an Allo-isoleucine A2 Analogue
Deposition date:
2001-12-27
Original release date:
2008-07-17
Authors:
Xu, Bin; Hua, Qing-xin; Nakagawa, Satoe; Jia, Wenhua; Chu, Ying-Chi; Katsoyannis, Panayotis; Weiss, Michael
Citation:

Citation: Xu, Bin; Hua, Qing-xin; Nakagawa, Satoe; Jia, Wenhua; Chu, Ying-Chi; Katsoyannis, Panayotis; Weiss, Michael. "Chiral Mutagenesis of Insulin's Hidden Receptor-binding Surface: Structure of an Allo-isoleucine(A2) Analogue "  J. Mol. Biol. 316, 435-441 (2002).
PubMed: 11866509

Assembly members:

Assembly members:
human insulin, polymer, 21 residues, Formula weight is not available
human insulin, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human insulin: GXVEQCCTSICSLYQLENYC N
human insulin: FVNQHLCGSDLVEALYLVCG ERGFFYTKPT

Data sets:
Data typeCount
1H chemical shifts475

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1allo-IleA2-DKP-insulin, chain A1
2allo-IleA2-DKP-insulin, chain B2

Entities:

Entity 1, allo-IleA2-DKP-insulin, chain A 21 residues - Formula weight is not available

1   GLYIILVALGLUGLNCYSCYSTHRSERILE
2   CYSSERLEUTYRGLNLEUGLUASNTYRCYS
3   ASN

Entity 2, allo-IleA2-DKP-insulin, chain B 30 residues - Formula weight is not available

1   PHEVALASNGLNHISLEUCYSGLYSERASP
2   LEUVALGLUALALEUTYRLEUVALCYSGLY
3   GLUARGGLYPHEPHETYRTHRLYSPROTHR

Samples:

sample_1: human insulin 1.2 mM; human insulin 1.2 mM

cond_1: pH: 7.0; temperature: 298 K

cond_2: pD: 7.6; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1not availablenot available
2D 1H-1H TOCSYsample_1not availablenot available
2D 1H-1H DQF-COSYsample_1not availablenot available

Software:

VNMR v6.1B - NMR data processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15450 15454 15455 16608 17108 1760 1762 1760 1762
PDB