BMRB Entry 5676

Title:
1H chemical shifts of Pi4, a four disulfide bridged scorpion toxin active on potassium channels
Deposition date:
2003-01-24
Original release date:
2003-09-05
Authors:
Guijarro, J.; M'Barek, S.; Olamendi-Portugal, T.; Gomez-Lagunas, F.; Garnier, D.; Rochat, H.; Possani, L.; Sabatier, J.; Delepierre, M.
Citation:

Citation: M'Barek, S.; Mosbah, A.; Sandoz, G.; Fajloun, Z.; Olamendi-Portugal, T.; Rochat, H.; Sampieri, F.; Guijarro, J.; Mansuelle, P.; Delepierre, M.; De Waard, M.; Sabatier, J.. "Synthesis and Characterization of Pi4, a Scorpion Toxin from Pandinus imperator that acts on K+ Channels"  Eur. J. Biochem. 270, 3583-3592 (2003).
PubMed: 12919322

Assembly members:

Assembly members:
Pi4 scorpion toxin, polymer, 38 residues, 4180 Da.

Natural source:

Natural source:   Common Name: Scorpion   Taxonomy ID: 55084   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Pandinus imperator

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pi4 scorpion toxin: IEAIRCGGSRDCYRPCQKRT GCPNAKCINKTCKCYGCS

Data sets:
Data typeCount
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pi4 scorpion toxin1

Entities:

Entity 1, Pi4 scorpion toxin 38 residues - 4180 Da.

1   ILEGLUALAILEARGCYSGLYGLYSERARG
2   ASPCYSTYRARGPROCYSGLNLYSARGTHR
3   GLYCYSPROASNALALYSCYSILEASNLYS
4   THRCYSLYSCYSTYRGLYCYSSER

Samples:

sample_1: Pi4 scorpion toxin 2.5 mM; sodium acetate 5 mM; D2O 10%

sample_2: Pi4 scorpion toxin 3.2 mM; sodium acetate 5 mM; D2O 100%

sample_cond_1: ionic strength: 5 mM; pH: 4.0; pressure: 1 atm; temperature: 303 K

sample_cond_2: ionic strength: 5 mM; pH*: 4.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
P-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available

Software:

VNMR v6.1C - collection, raw spectral data processing

NMRView v5.03 - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
SWISS-PROT P58498