BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5703

Title: SP phosphorothioate U6 RNA ISL   PubMed: 12702812

Authors: Reiter, Nicholas; Butcher, Samuel

Citation: Reiter, Nicholas; Nikstad, Laura; Allmann, Anne; Johnson, Randal; Butcher, Samuel. "Structure of the U6 RNA Intramolecular Stem-loop Harboring an SP-phosphorothioate Modification"  RNA 9, 533-542 (2003).

Assembly members:
U6 snRNA, polymer, 24 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
U6 snRNA: GGUUCCCCUGCAUAAGGAXG AACC

Data sets:
Data typeCount
13C chemical shifts66
31P chemical shifts1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1U6 snRNA1

Entities:

Entity 1, U6 snRNA 24 residues - Formula weight is not available

1   GGUUCCCCUG
2   CAUAAGGASSUG
3   AACC

Samples:

sample_1: U6 snRNA 1 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_2: U6 snRNA 1 mM; NaCl 50 mM; D2O 100%

sample_3: U6 snRNA 1 mM; NaCl 50 mM; D2O 100%; Pf1 phage 17 mg/mL

cond_1: ionic strength: 0.05 M; pH: 7.0; temperature: 283 K

cond_2: ionic strength: 0.05 M; pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

xwinnmr v2.6 -

SPARKY v2.6 -

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 500 MHz
  • Bruker DMX 400 MHz

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