BMRB Entry 5972

Title:
Proton Chemical Shifts for E coli ProP 468-497 peptide
Deposition date:
2003-10-14
Original release date:
2003-12-19
Authors:
Zoetewey, David; Tripet, Brian; Kutateladze, Tatiana; Overduin, Michael; Wood, Janet; Hodges, Robert
Citation:

Citation: Zoetewey, David; Tripet, Brian; Kutateladze, Tatiana; Overduin, Michael; Wood, Janet; Hodges, Robert. "Solution Structure of the C-terminal Antiparallel Coiled-coil Domain of Escherichia Coli Osmosensor ProP "  J. Mol. Biol. 334, 1063-1076 (2003).
PubMed: 14643666

Assembly members:

Assembly members:
ProP, polymer, 36 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ProP: XXCGGDNIEQKIDDIDHEIA DLQAKRTRLVQQHPRX

Data sets:
Data typeCount
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ProP subunit A, ProP 468-497 monomer peptide1
2ProP subunit B, ProP 468-497 monomer peptide1

Entities:

Entity 1, ProP subunit A, ProP 468-497 monomer peptide 36 residues - Formula weight is not available

1   ACECAMCYSGLYGLYASPASNILEGLUGLN
2   LYSILEASPASPILEASPHISGLUILEALA
3   ASPLEUGLNALALYSARGTHRARGLEUVAL
4   GLNGLNHISPROARGNH2

Samples:

sample_1: ProP 3.0 mM; potassium phosphate 50 mM; KCl 100 mM; D2O 10%; H2O 90%

H2O_condition: pH: 5.0; temperature: 298 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availableH2O_condition
TOCSYsample_1not availableH2O_condition
COSYsample_1not availableH2O_condition

Software:

NMRPipe - FT, phasing

CNS v1.1 - simulated annealing

NMRView v5.0.4 - Semi-automated Peakpicking

NMR spectrometers:

  • Varian Oxford 600 MHz
  • Varian Oxford 500 MHz

Related Database Links:

PDB
DBJ BAB38516 BAE78113 BAI57531 BAJ45826 BAL40666
EMBL CAP78584 CAQ34460 CAR05769 CAR10804 CAR15757
GB AAA97010 AAB00919 AAC44538 AAC77072 AAG59310
REF NP_290744 NP_313120 NP_418535 NP_756966 WP_000530019
SP P0C0L7 P0C0L8
AlphaFold P0C0L7 P0C0L8