BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6257

Title: Alzheimer's Abeta40 Studied by NMR at Low pH Reveals That DSS Binds and Promotes beta-ball Oligomerization   PubMed: 15557279

Authors: Laurents, Douglas; Gorman, Paul; Guo, Ming; Rico, Manuel; Chakrabartty, Avijit; Bruix, Marta

Citation: Laurents, Douglas; Gorman, Paul; Guo, Ming; Rico, Manuel; Chakrabartty, Avijit; Bruix, Marta. "Alzheimer's Abeta40 Studied by NMR at Low pH Reveals That DSS Binds and Promotes beta-ball Oligomerization"  J. Biol. Chem. 280, 3675-3685 (2004).

Assembly members:
Abeta(1-40), polymer, 40 residues, 4300 Da.

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Abeta(1-40): DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV

Data sets:
Data typeCount
1H chemical shifts279
13C chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Abeta(1-40)1

Entities:

Entity 1, Abeta(1-40) 40 residues - 4300 Da.

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL

Samples:

sample_1: Abeta(1-40)0.15 – 0.30 mM; NaH2PO4 5 mM

Ex-cond_1: pH: 2.5; temperature: 298 K; ionic strength: 0.01 M

Ex-cond_2: pH*: 2.5; temperature: 298 K; ionic strength: 0.01 M

Experiments:

NameSampleSample stateSample conditions
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
COSYnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available

Software:

UXWINNMR -

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 11435 15775 17159 17186 17764 17793 17794 17795 17796 18052 18127 18128 18129 18131 19009 19309 19393 25289 5057 5400 6554
PDB
DBJ BAA22264 BAA84580 BAB71958 BAD51938 BAE01907
EMBL CAA30050 CAA31830 CAA39589 CAA39590 CAA39591
GB AAA35540 AAA36829 AAA51564 AAA51722 AAA51726
PIR A60045 D60045 E60045 G60045 PQ0438
PRF 1303338A 1403400A 1405204A 1507304A 1507304B
REF NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_001127014
SP P05067 P53601 P79307 P86906 Q28053
TPG DAA33655