BMRB Entry 6573

Title:
NMR Structure of VPS4A MIT Domain
Deposition date:
2005-03-31
Original release date:
2005-10-17
Authors:
Scott, J.; Gaspar, J.; Stuchell, M.; Alam, S.; Skalicky, J.; Sundquist, W.
Citation:

Citation: Scott, A.; Gaspar, J.; Stuchell-Brereton, M.; Alam, S.; Skalicky, J.; Sundquist, W.. "Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A."  Proc. Natl. Acad. Sci. U. S. A. 102, 13813-13818 (2005).
PubMed: 16174732

Assembly members:

Assembly members:
vacuolar protein sorting factor 4A, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts869
13C chemical shifts402
15N chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1vacuolar protein sorting factor 4A1

Entities:

Entity 1, vacuolar protein sorting factor 4A 122 residues - Formula weight is not available

1   METTHRTHRSERTHRLEUGLNLYSALAILE
2   ASPLEUVALTHRLYSALATHRGLUGLUASP
3   LYSALALYSASNTYRGLUGLUALALEUARG
4   LEUTYRGLNHISALAVALGLUTYRPHELEU
5   HISALAILELYSTYRGLUALAHISSERASP
6   LYSALALYSGLUSERILEARGALALYSCYS
7   VALGLNTYRLEUASPARGALAGLULYSLEU
8   LYSASPTYRLEUARGSERLYSGLULYSHIS
9   GLYLYSLYSPROVALLYSGLUASNGLNSER
10   GLUGLYLYSGLYSERASPSERASPSERGLU
11   GLYASPASNPROGLULYSLYSLYSLEUGLN
12   GLUGLNLEUMETGLYALAVALVALMETGLU
13   LYSPRO

Samples:

sample_1: vacuolar protein sorting factor 4A, [U-13C; U-15N], mM; NaCl 50 mM; sodium phosphate 20 mM; D2O 10%

sample_cond_1: pH: 5.5; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

FELIX v97 - processing

Sparky v3.110 - data analysis

CYANA v2.0 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAC00961 BAC33165 BAE34833 BAE41476 BAG37514
GB AAD42971 AAD49227 AAF17203 AAG01470 AAH18368
REF NP_001040080 NP_001253930 NP_037377 NP_569053 NP_663711
SP Q793F9 Q8VEJ9 Q9UN37
TPG DAA20141
AlphaFold Q793F9 Q8VEJ9 Q9UN37

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks