BMRB Entry 6631

Title:
Solution structure of a human ubiquitin-like domain in SF3A1
Deposition date:
2005-05-12
Original release date:
2007-11-13
Authors:
Lukin, J.; Dhe-Paganon, S.; Guido, V.; Arrowsmith, C.
Citation:

Citation: Avvakumov, G.; Xue, S.; Newman, E.; Mackenzie, F.; Dhe-Paganon, S.. "Solution structure of a human ubiquitin-like domain in SF3A1"  .

Assembly members:

Assembly members:
Splicing factor 3, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts587
13C chemical shifts365
15N chemical shifts91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Splicing factor 3 subunit 11

Entities:

Entity 1, Splicing factor 3 subunit 1 86 residues - Formula weight is not available

1   PROVALSERILELYSVALGLNVALPROASN
2   METGLNASPLYSTHRGLUTRPLYSLEUASN
3   GLYGLNVALLEUVALPHETHRLEUPROLEU
4   THRASPGLNVALSERVALILELYSVALLYS
5   ILEHISGLUALATHRGLYMETPROALAGLY
6   LYSGLNLYSLEUGLNTYRGLUGLYILEPHE
7   ILELYSASPSERASNSERLEUALATYRTYR
8   ASNMETALAASNGLYALAVALILEHISLEU
9   ALALEULYSGLUARGGLY

Samples:

sample_1: Splicing factor 3, [U-15N; U-13C], 2 mM; NaCl 300 mM; benzamidine 1 mM; DTT 10 mM; NaN3 0.01%; Sodium Acetate 10 mM; ZnSO4 0.01 mM; H2O 90%; D2O 10%

sample_2: Splicing factor 3, [U-15N; U-13C], 2 mM; NaCl 300 mM; benzamidine 1 mM; DTT 10 mM; NaN3 0.01%; Sodium Acetate 10 mM; ZnSO4 0.01 mM; D2O 99.9%

sample_cond_1: pH: 5.0; temperature: 298 K; ionic strength: 300 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

XWINNMR v3.6 - collection

NMRPipe v2.1 - processing

CYANA v2.0 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAC26142 BAC26294 BAG64353 BAG72797 BAK62823
EMBL CAA59494 CAG30461 CAK54605 CAK54904
GB AAC23435 AAH01976 AAH07684 AAH10727 AAH29753
REF NP_001074979 NP_001100705 NP_001244628 NP_001267465 NP_005868
SP A2VDN6 Q15459 Q8K4Z5
TPG DAA20517
AlphaFold Q8K4Z5 A2VDN6 Q15459

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks