BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6906

Title: 1H, 13C, and 15N Chemical Shift Assignments for the Bicoid Homedomain   PubMed: 16406070

Authors: Baird-Titus, Jamie; Clark-Baldwin, Kimber; Dave, Vrushank; Caperelli, Carol; Ma, Jun; Rance, Mark

Citation: Baird-Titus, Jamie; Clark-Baldwin, Kimber; Dave, Vrushank; Caperelli, Carol; Ma, Jun; Rance, Mark. "The solution structure of the native K50 Bicoid homeodomain bound to the consensus TAATCC DNA-binding site"  J. Mol. Biol. 356, 1137-1151 (2006).

Assembly members:
protein, polymer, 68 residues, Formula weight is not available
DNA Sense Strand, polymer, 13 residues, Formula weight is not available
DNA Anti-Sense Strand, polymer, 13 residues, Formula weight is not available

Natural source:   Common Name: Fruit Fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila Melanogaster

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
protein: GPRRTRTTFTSSQIAELEQH FLQGRYLTAPRLADLSAKLA LGTAQVKIWFKNRRRRHKIQ SDQHKDQS
DNA Sense Strand: GCTCTAATCCCCG
DNA Anti-Sense Strand: CGAGATTAGGGGC

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts76
1H chemical shifts478

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bicoid Homeodomain1
2DNA Sense Strand2
3DNA Anti-sense strand3

Entities:

Entity 1, Bicoid Homeodomain 68 residues - Formula weight is not available

1   GLYPROARGARGTHRARGTHRTHRPHETHR
2   SERSERGLNILEALAGLULEUGLUGLNHIS
3   PHELEUGLNGLYARGTYRLEUTHRALAPRO
4   ARGLEUALAASPLEUSERALALYSLEUALA
5   LEUGLYTHRALAGLNVALLYSILETRPPHE
6   LYSASNARGARGARGARGHISLYSILEGLN
7   SERASPGLNHISLYSASPGLNSER

Entity 2, DNA Sense Strand 13 residues - Formula weight is not available

1   DGDCDTDCDTDADADTDCDC
2   DCDCDG

Entity 3, DNA Anti-sense strand 13 residues - Formula weight is not available

1   DCDGDADGDADTDTDADGDG
2   DGDGDC

Samples:

sample_1: Bicoid Homeodomain, [U-95% 13C; U-90% 15N], 0.8 ± 0.2 mM; DNA Sense Strand, unlabeled, 0.8 ± 0.2 mM; DNA Anti-sense strand, unlabeled, 0.8 ± 0.2 mM; Phosphate buffer 10 mM; DTT 1 mM; PMSF 1 mM; Roche Complete Protease Inhibitor Cocktail; Leupeptin 0.3 mM; Roche PefaBloc 0.2 mM; EDTA 1 mM; sodium azide 0.1 mM; D2O 10%; H2O 90%

conditions_1: pH: 7.0; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
3D-HNCAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCACOsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HBHA(CBCACO)NHsample_1not availableconditions_1
H(CCO)NH-TOCSYsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
15N-separated NOESYsample_1not availableconditions_1
13C-separated NOESYsample_1not availableconditions_1
2D-15N HSQCsample_1not availableconditions_1
15N HMQCsample_1not availableconditions_1
HMQC-TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAA30720 CAA32627 CAA39267 CAB37631
GB AAA28391 AAD19798 AAF54085 AAL13887 AAL73985
REF NP_731111 NP_731113 NP_788587 NP_788588 XP_001359217
SP P09081