BMRB Entry 7157

Title:
NMR spectroscopy of T4 Lysozyme peptide fragments
Deposition date:
2006-06-07
Original release date:
2008-11-12
Authors:
Najbar, L.; Craik, D.; Wade, J.; McLeish, M.
Citation:

Citation: Najbar, L.; Craik, D.; Wade, J.; McLeish, M.. "Identification of initiation sites for T4 lysozyme folding using CD and NMR spectroscopy of peptide fragments"  Biochemistry 39, 5911-5920 (2000).
PubMed: 10801343

Assembly members:

Assembly members:
helix F (lys 113-125), polymer, 13 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
helix F (lys 113-125): GFTNSLRMLQQKR

Data sets:
Data typeCount
1H chemical shifts294

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fragment corresponding to Lys Helix F (residues 113-125)1

Entities:

Entity 1, fragment corresponding to Lys Helix F (residues 113-125) 13 residues - Formula weight is not available

1   GLYPHETHRASNSERLEUARGMETLEUGLN
2   GLNLYSARG

Samples:

sample_1: helix F (lys 113-125)1 – 2 mM; H2O 90%; D2O 10%

sample_2: helix F (lys 113-125)1 – 2 mM; TFE 50%; H2O 40%; D2O 10%

sample_3: helix F (lys 113-125)1 – 2 mM; H2O 90%; D2O 10%; SDS 200 mM

conditions_1: pH: . .; temperature: 278 K

conditions_2: pH: . .; temperature: 283 K

conditions_3: pH: . .; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers: