data_16244 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone NH Assignments for Prp24-RRM23 Bound to AGAGAU RNA ; _BMRB_accession_number 16244 _BMRB_flat_file_name bmr16244.str _Entry_type new _Submission_date 2009-04-07 _Accession_date 2009-04-07 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Martin-Tumasz Stephen A. . 2 Butcher Samuel E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 131 "15N chemical shifts" 131 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2009-11-13 update BMRB 'complete entry citation' 2009-09-04 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 7070 'PRP24 (RRM 1 and 2)' 16230 'Prp24-RRM2 bound to AGAGAU from U6 snRNA' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title '(1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 19693704 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Martin-Tumasz Stephen . . 2 Butcher Samuel E. . stop_ _Journal_abbreviation 'Biomol. NMR Assignments' _Journal_name_full 'Biomolecular NMR assignments' _Journal_volume 3 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 227 _Page_last 230 _Year 2009 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'U6 snRNP (fragment)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Prp24-RRM23 $Prp24-RRM23 AGAGAU $AGAGAU stop_ _System_molecular_weight 23350 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details 'Prp24-RRM23 bound to AGAGAU RNA (from U6 snRNA)' save_ ######################## # Monomeric polymers # ######################## save_Prp24-RRM23 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Prp24-RRM23 _Molecular_mass 21430 _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 186 _Mol_residue_sequence ; MTECTLWMTNFPPSYTQRNI RDLLQDINVVALSIRLPSLR FNTSRRFAYIDVTSKEDARY CVEKLNGLKIEGYTLVTKVS NPLEKSKRTDSATLEGREIM IRNLSTELLDENLLRESFEG FGSIEKINIPAGQKEHSFNN CCAFMVFENKDSAERALQMN RSLLGNREISVSLADKKPLE HHHHHH ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 114 MET 2 115 THR 3 116 GLU 4 117 CYS 5 118 THR 6 119 LEU 7 120 TRP 8 121 MET 9 122 THR 10 123 ASN 11 124 PHE 12 125 PRO 13 126 PRO 14 127 SER 15 128 TYR 16 129 THR 17 130 GLN 18 131 ARG 19 132 ASN 20 133 ILE 21 134 ARG 22 135 ASP 23 136 LEU 24 137 LEU 25 138 GLN 26 139 ASP 27 140 ILE 28 141 ASN 29 142 VAL 30 143 VAL 31 144 ALA 32 145 LEU 33 146 SER 34 147 ILE 35 148 ARG 36 149 LEU 37 150 PRO 38 151 SER 39 152 LEU 40 153 ARG 41 154 PHE 42 155 ASN 43 156 THR 44 157 SER 45 158 ARG 46 159 ARG 47 160 PHE 48 161 ALA 49 162 TYR 50 163 ILE 51 164 ASP 52 165 VAL 53 166 THR 54 167 SER 55 168 LYS 56 169 GLU 57 170 ASP 58 171 ALA 59 172 ARG 60 173 TYR 61 174 CYS 62 175 VAL 63 176 GLU 64 177 LYS 65 178 LEU 66 179 ASN 67 180 GLY 68 181 LEU 69 182 LYS 70 183 ILE 71 184 GLU 72 185 GLY 73 186 TYR 74 187 THR 75 188 LEU 76 189 VAL 77 190 THR 78 191 LYS 79 192 VAL 80 193 SER 81 194 ASN 82 195 PRO 83 196 LEU 84 197 GLU 85 198 LYS 86 199 SER 87 200 LYS 88 201 ARG 89 202 THR 90 203 ASP 91 204 SER 92 205 ALA 93 206 THR 94 207 LEU 95 208 GLU 96 209 GLY 97 210 ARG 98 211 GLU 99 212 ILE 100 213 MET 101 214 ILE 102 215 ARG 103 216 ASN 104 217 LEU 105 218 SER 106 219 THR 107 220 GLU 108 221 LEU 109 222 LEU 110 223 ASP 111 224 GLU 112 225 ASN 113 226 LEU 114 227 LEU 115 228 ARG 116 229 GLU 117 230 SER 118 231 PHE 119 232 GLU 120 233 GLY 121 234 PHE 122 235 GLY 123 236 SER 124 237 ILE 125 238 GLU 126 239 LYS 127 240 ILE 128 241 ASN 129 242 ILE 130 243 PRO 131 244 ALA 132 245 GLY 133 246 GLN 134 247 LYS 135 248 GLU 136 249 HIS 137 250 SER 138 251 PHE 139 252 ASN 140 253 ASN 141 254 CYS 142 255 CYS 143 256 ALA 144 257 PHE 145 258 MET 146 259 VAL 147 260 PHE 148 261 GLU 149 262 ASN 150 263 LYS 151 264 ASP 152 265 SER 153 266 ALA 154 267 GLU 155 268 ARG 156 269 ALA 157 270 LEU 158 271 GLN 159 272 MET 160 273 ASN 161 274 ARG 162 275 SER 163 276 LEU 164 277 LEU 165 278 GLY 166 279 ASN 167 280 ARG 168 281 GLU 169 282 ILE 170 283 SER 171 284 VAL 172 285 SER 173 286 LEU 174 287 ALA 175 288 ASP 176 289 LYS 177 290 LYS 178 291 PRO 179 292 LEU 180 293 GLU 181 294 HIS 182 295 HIS 183 296 HIS 184 297 HIS 185 298 HIS 186 299 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-21 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 16243 Prp24-RRM23 100.00 186 100.00 100.00 1.46e-134 BMRB 17589 RRM3 50.00 93 97.85 98.92 5.47e-58 PDB 2GHP "Crystal Structure Of The N-Terminal 3 Rna Binding Domains Of The Yeast Splicing Factor Prp24" 95.70 292 97.19 97.75 4.79e-121 PDB 4N0T "Core Structure Of The U6 Small Nuclear Ribonucleoprotein At 1.7 Angstrom Resolution" 96.24 374 98.88 99.44 4.78e-124 DBJ GAA25719 "K7_Prp24p [Saccharomyces cerevisiae Kyokai no. 7]" 96.24 444 98.32 99.44 1.38e-122 EMBL CAA89251 "U6sNRPp [Saccharomyces cerevisiae]" 96.24 444 98.88 99.44 6.26e-123 EMBL CAY82100 "Prp24p [Saccharomyces cerevisiae EC1118]" 96.24 444 98.88 99.44 6.26e-123 GB AAU09775 "YMR268C [Saccharomyces cerevisiae]" 96.24 444 98.32 98.88 6.68e-122 GB AHY76723 "Prp24p [Saccharomyces cerevisiae YJM993]" 96.24 444 98.88 99.44 6.26e-123 GB AJP40962 "Prp24p [Saccharomyces cerevisiae YJM1078]" 96.24 444 98.32 98.88 4.88e-122 GB AJS62134 "Prp24p [Saccharomyces cerevisiae YJM189]" 96.24 444 98.88 99.44 6.26e-123 GB AJS62569 "Prp24p [Saccharomyces cerevisiae YJM193]" 96.24 444 98.88 99.44 6.46e-123 REF NP_013995 "U6 snRNP complex subunit PRP24 [Saccharomyces cerevisiae S288c]" 96.24 444 98.88 99.44 6.26e-123 SP P49960 "RecName: Full=U4/U6 snRNA-associated-splicing factor PRP24; Short=U4/U6 snRNP protein" 96.24 444 98.88 99.44 6.26e-123 TPG DAA10168 "TPA: U6 snRNP complex subunit PRP24 [Saccharomyces cerevisiae S288c]" 96.24 444 98.88 99.44 6.26e-123 stop_ save_ save_AGAGAU _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common AGAGAU _Molecular_mass 1922 _Mol_thiol_state 'not present' _Details . _Residue_count 6 _Mol_residue_sequence AGAGAU loop_ _Residue_seq_code _Residue_label 1 A 2 G 3 A 4 G 5 A 6 U stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Prp24-RRM23 'baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae $AGAGAU 'baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Prp24-RRM23 'recombinant technology' . . . BL21 pET21-b $AGAGAU 'obtained from a vendor' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_Fully_Labeled _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Prp24-RRM23 500 uM '[U-99% 13C; U-99% 15N]' TRIS 10 mM 'natural abundance' 'potassium chloride' 50 mM 'natural abundance' DTT 1 mM 'natural abundance' $AGAGAU 5 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_Referencing _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Prp24-RRM23 500 uM '[U-99% 13C; U-99% 15N]' TRIS 10 mM 'natural abundance' 'potassium chloride' 50 mM 'natural abundance' DTT 1 mM 'natural abundance' DSS 10 uM 'natural abundance' $AGAGAU 5 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRDraw _Saveframe_category software _Name NMRDraw _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'peak picking' 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_600 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $Fully_Labeled save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $Fully_Labeled save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $Fully_Labeled save_ save_3D_HNCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $Fully_Labeled save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298.15 . K pH 7 . pH pressure 1 . atm 'ionic strength' 50 . mM stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS C 13 'methyl protons' ppm 0.00 n/a indirect . . . 0.251449530 DSS N 15 'methyl protons' ppm 0.00 n/a indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $SPARKY stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCACB' '3D HNCA' stop_ loop_ _Sample_label $Fully_Labeled stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Prp24-RRM23 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 119 6 LEU H H 10.03 0.02 1 2 119 6 LEU N N 127.03 0.4 1 3 120 7 TRP H H 9.321 0.02 1 4 120 7 TRP N N 122.74 0.4 1 5 122 9 THR H H 8.47 0.02 1 6 122 9 THR N N 115.912 0.4 1 7 123 10 ASN H H 7.921 0.02 1 8 123 10 ASN N N 119.108 0.4 1 9 124 11 PHE H H 5.965 0.02 1 10 124 11 PHE N N 108.63 0.4 1 11 127 14 SER H H 7.52 0.02 1 12 127 14 SER N N 109.306 0.4 1 13 128 15 TYR H H 7.979 0.02 1 14 128 15 TYR N N 124.542 0.4 1 15 129 16 THR H H 8.727 0.02 1 16 129 16 THR N N 112.665 0.4 1 17 130 17 GLN H H 9.241 0.02 1 18 130 17 GLN N N 118.81 0.4 1 19 132 19 ASN H H 7.032 0.02 1 20 132 19 ASN N N 114.78 0.4 1 21 133 20 ILE H H 7.187 0.02 1 22 133 20 ILE N N 119.086 0.4 1 23 134 21 ARG H H 8.206 0.02 1 24 134 21 ARG N N 120.805 0.4 1 25 136 23 LEU H H 7.407 0.02 1 26 136 23 LEU N N 121.274 0.4 1 27 137 24 LEU H H 7.309 0.02 1 28 137 24 LEU N N 117.656 0.4 1 29 138 25 GLN H H 8.51 0.02 1 30 138 25 GLN N N 119.939 0.4 1 31 139 26 ASP H H 7.765 0.02 1 32 139 26 ASP N N 119.486 0.4 1 33 140 27 ILE H H 7.232 0.02 1 34 140 27 ILE N N 110.26 0.4 1 35 141 28 ASN H H 8.15 0.02 1 36 141 28 ASN N N 116.891 0.4 1 37 142 29 VAL H H 8.09 0.02 1 38 142 29 VAL N N 119.61 0.4 1 39 143 30 VAL H H 8.46 0.02 1 40 143 30 VAL N N 127.453 0.4 1 41 144 31 ALA H H 8.427 0.02 1 42 144 31 ALA N N 128.827 0.4 1 43 145 32 LEU H H 8.835 0.02 1 44 145 32 LEU N N 123.986 0.4 1 45 146 33 SER H H 7.206 0.02 1 46 146 33 SER N N 110.433 0.4 1 47 147 34 ILE H H 8.375 0.02 1 48 147 34 ILE N N 125.352 0.4 1 49 148 35 ARG H H 9.447 0.02 1 50 148 35 ARG N N 127.354 0.4 1 51 149 36 LEU H H 8.674 0.02 1 52 149 36 LEU N N 126.29 0.4 1 53 151 38 SER H H 8.488 0.02 1 54 151 38 SER N N 116.597 0.4 1 55 152 39 LEU H H 8.341 0.02 1 56 152 39 LEU N N 124.152 0.4 1 57 153 40 ARG H H 8.533 0.02 1 58 153 40 ARG N N 121.883 0.4 1 59 161 48 ALA H H 8.836 0.02 1 60 161 48 ALA N N 119.877 0.4 1 61 162 49 TYR H H 8.758 0.02 1 62 162 49 TYR N N 117.275 0.4 1 63 163 50 ILE H H 9.087 0.02 1 64 163 50 ILE N N 121.686 0.4 1 65 164 51 ASP H H 8.786 0.02 1 66 164 51 ASP N N 127.508 0.4 1 67 165 52 VAL H H 8.98 0.02 1 68 165 52 VAL N N 115.456 0.4 1 69 166 53 THR H H 9.297 0.02 1 70 166 53 THR N N 111.634 0.4 1 71 171 58 ALA H H 7.919 0.02 1 72 171 58 ALA N N 122.919 0.4 1 73 172 59 ARG H H 8.127 0.02 1 74 172 59 ARG N N 117.214 0.4 1 75 173 60 TYR H H 8.157 0.02 1 76 173 60 TYR N N 121.497 0.4 1 77 174 61 CYS H H 8.298 0.02 1 78 174 61 CYS N N 115.67 0.4 1 79 175 62 VAL H H 7.848 0.02 1 80 175 62 VAL N N 119.483 0.4 1 81 176 63 GLU H H 7.946 0.02 1 82 176 63 GLU N N 118.564 0.4 1 83 177 64 LYS H H 7.954 0.02 1 84 177 64 LYS N N 114.002 0.4 1 85 178 65 LEU H H 8.389 0.02 1 86 178 65 LEU N N 115.567 0.4 1 87 179 66 ASN H H 7.97 0.02 1 88 179 66 ASN N N 116.316 0.4 1 89 180 67 GLY H H 8.968 0.02 1 90 180 67 GLY N N 116.989 0.4 1 91 181 68 LEU H H 7.439 0.02 1 92 181 68 LEU N N 123.752 0.4 1 93 182 69 LYS H H 8.248 0.02 1 94 182 69 LYS N N 125.634 0.4 1 95 183 70 ILE H H 9.032 0.02 1 96 183 70 ILE N N 126.322 0.4 1 97 184 71 GLU H H 9.203 0.02 1 98 184 71 GLU N N 123.956 0.4 1 99 185 72 GLY H H 8.273 0.02 1 100 185 72 GLY N N 103.599 0.4 1 101 186 73 TYR H H 8.452 0.02 1 102 186 73 TYR N N 123.175 0.4 1 103 187 74 THR H H 8.277 0.02 1 104 187 74 THR N N 119.064 0.4 1 105 188 75 LEU H H 9.096 0.02 1 106 188 75 LEU N N 131.261 0.4 1 107 189 76 VAL H H 8.032 0.02 1 108 189 76 VAL N N 125.277 0.4 1 109 202 89 THR H H 8.243 0.02 1 110 202 89 THR N N 115.42 0.4 1 111 203 90 ASP H H 8.388 0.02 1 112 203 90 ASP N N 122.956 0.4 1 113 204 91 SER H H 8.247 0.02 1 114 204 91 SER N N 116.806 0.4 1 115 205 92 ALA H H 8.361 0.02 1 116 205 92 ALA N N 124.797 0.4 1 117 206 93 THR H H 7.897 0.02 1 118 206 93 THR N N 112.673 0.4 1 119 207 94 LEU H H 8.114 0.02 1 120 207 94 LEU N N 123.404 0.4 1 121 212 99 ILE H H 9.444 0.02 1 122 212 99 ILE N N 120.993 0.4 1 123 213 100 MET H H 9.297 0.02 1 124 213 100 MET N N 122.222 0.4 1 125 214 101 ILE H H 8.911 0.02 1 126 214 101 ILE N N 129.043 0.4 1 127 215 102 ARG H H 9.189 0.02 1 128 215 102 ARG N N 123.126 0.4 1 129 216 103 ASN H H 8.327 0.02 1 130 216 103 ASN N N 114.267 0.4 1 131 217 104 LEU H H 8.021 0.02 1 132 217 104 LEU N N 115.432 0.4 1 133 218 105 SER H H 6.463 0.02 1 134 218 105 SER N N 114.278 0.4 1 135 219 106 THR H H 8.952 0.02 1 136 219 106 THR N N 112.809 0.4 1 137 220 107 GLU H H 8.315 0.02 1 138 220 107 GLU N N 119.606 0.4 1 139 221 108 LEU H H 7.254 0.02 1 140 221 108 LEU N N 118.157 0.4 1 141 222 109 LEU H H 6.936 0.02 1 142 222 109 LEU N N 118.341 0.4 1 143 223 110 ASP H H 7.819 0.02 1 144 223 110 ASP N N 124.773 0.4 1 145 224 111 GLU H H 9.465 0.02 1 146 224 111 GLU N N 126.614 0.4 1 147 225 112 ASN H H 8.245 0.02 1 148 225 112 ASN N N 116.892 0.4 1 149 226 113 LEU H H 7.893 0.02 1 150 226 113 LEU N N 122.826 0.4 1 151 230 117 SER H H 7.821 0.02 1 152 230 117 SER N N 111.887 0.4 1 153 231 118 PHE H H 7.835 0.02 1 154 231 118 PHE N N 116.635 0.4 1 155 232 119 GLU H H 8.751 0.02 1 156 232 119 GLU N N 120.594 0.4 1 157 233 120 GLY H H 8.57 0.02 1 158 233 120 GLY N N 108.716 0.4 1 159 234 121 PHE H H 7.755 0.02 1 160 234 121 PHE N N 117.216 0.4 1 161 235 122 GLY H H 7.559 0.02 1 162 235 122 GLY N N 106.468 0.4 1 163 236 123 SER H H 8.212 0.02 1 164 236 123 SER N N 110.838 0.4 1 165 237 124 ILE H H 9.026 0.02 1 166 237 124 ILE N N 129.599 0.4 1 167 239 126 LYS H H 7.497 0.02 1 168 239 126 LYS N N 116.093 0.4 1 169 240 127 ILE H H 8.587 0.02 1 170 240 127 ILE N N 124.085 0.4 1 171 241 128 ASN H H 9.211 0.02 1 172 241 128 ASN N N 125.673 0.4 1 173 242 129 ILE H H 8.901 0.02 1 174 242 129 ILE N N 127.462 0.4 1 175 244 131 ALA H H 8.729 0.02 1 176 244 131 ALA N N 126.97 0.4 1 177 245 132 GLY H H 8.608 0.02 1 178 245 132 GLY N N 107.887 0.4 1 179 246 133 GLN H H 7.947 0.02 1 180 246 133 GLN N N 118.267 0.4 1 181 247 134 LYS H H 8.15 0.02 1 182 247 134 LYS N N 117.586 0.4 1 183 248 135 GLU H H 8.797 0.02 1 184 248 135 GLU N N 119.486 0.4 1 185 249 136 HIS H H 7.266 0.02 1 186 249 136 HIS N N 115.101 0.4 1 187 253 140 ASN H H 8.13 0.02 1 188 253 140 ASN N N 118.496 0.4 1 189 254 141 CYS H H 8.914 0.02 1 190 254 141 CYS N N 121.161 0.4 1 191 255 142 CYS H H 9.617 0.02 1 192 255 142 CYS N N 118.094 0.4 1 193 256 143 ALA H H 8.436 0.02 1 194 256 143 ALA N N 119.328 0.4 1 195 257 144 PHE H H 8.756 0.02 1 196 257 144 PHE N N 117.783 0.4 1 197 258 145 MET H H 8.758 0.02 1 198 258 145 MET N N 121.312 0.4 1 199 259 146 VAL H H 8.923 0.02 1 200 259 146 VAL N N 125.906 0.4 1 201 260 147 PHE H H 9.152 0.02 1 202 260 147 PHE N N 126.73 0.4 1 203 261 148 GLU H H 8.349 0.02 1 204 261 148 GLU N N 119.357 0.4 1 205 262 149 ASN H H 8.293 0.02 1 206 262 149 ASN N N 112.244 0.4 1 207 263 150 LYS H H 9.023 0.02 1 208 263 150 LYS N N 122.32 0.4 1 209 264 151 ASP H H 8.613 0.02 1 210 264 151 ASP N N 119.694 0.4 1 211 265 152 SER H H 7.708 0.02 1 212 265 152 SER N N 116.086 0.4 1 213 266 153 ALA H H 6.537 0.02 1 214 266 153 ALA N N 122.136 0.4 1 215 267 154 GLU H H 7.675 0.02 1 216 267 154 GLU N N 113.296 0.4 1 217 268 155 ARG H H 7.803 0.02 1 218 268 155 ARG N N 119.839 0.4 1 219 269 156 ALA H H 7.522 0.02 1 220 269 156 ALA N N 120.919 0.4 1 221 270 157 LEU H H 7.158 0.02 1 222 270 157 LEU N N 115.404 0.4 1 223 271 158 GLN H H 7.849 0.02 1 224 271 158 GLN N N 119.19 0.4 1 225 272 159 MET H H 7.727 0.02 1 226 272 159 MET N N 114.882 0.4 1 227 273 160 ASN H H 7.922 0.02 1 228 273 160 ASN N N 119.181 0.4 1 229 274 161 ARG H H 8.915 0.02 1 230 274 161 ARG N N 121.292 0.4 1 231 275 162 SER H H 7.828 0.02 1 232 275 162 SER N N 114.939 0.4 1 233 276 163 LEU H H 8.148 0.02 1 234 276 163 LEU N N 120.168 0.4 1 235 277 164 LEU H H 9.086 0.02 1 236 277 164 LEU N N 129.3 0.4 1 237 278 165 GLY H H 8.896 0.02 1 238 278 165 GLY N N 114.429 0.4 1 239 279 166 ASN H H 8.347 0.02 1 240 279 166 ASN N N 120.308 0.4 1 241 280 167 ARG H H 8.083 0.02 1 242 280 167 ARG N N 119.633 0.4 1 243 281 168 GLU H H 8.383 0.02 1 244 281 168 GLU N N 122.767 0.4 1 245 282 169 ILE H H 8.612 0.02 1 246 282 169 ILE N N 122.928 0.4 1 247 283 170 SER H H 8.028 0.02 1 248 283 170 SER N N 115.898 0.4 1 249 284 171 VAL H H 8.968 0.02 1 250 284 171 VAL N N 126.282 0.4 1 251 285 172 SER H H 8.492 0.02 1 252 285 172 SER N N 119.093 0.4 1 253 286 173 LEU H H 8.885 0.02 1 254 286 173 LEU N N 122.153 0.4 1 255 287 174 ALA H H 8.573 0.02 1 256 287 174 ALA N N 126.929 0.4 1 257 288 175 ASP H H 8.508 0.02 1 258 288 175 ASP N N 120.161 0.4 1 259 289 176 LYS H H 8.233 0.02 1 260 289 176 LYS N N 121.113 0.4 1 261 290 177 LYS H H 8.361 0.02 1 262 290 177 LYS N N 124.58 0.4 1 stop_ save_