data_16754 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Physical Interaction between Replication Protein A and Rad51 Promotes Exchange on Single-stranded DNA ; _BMRB_accession_number 16754 _BMRB_flat_file_name bmr16754.str _Entry_type original _Submission_date 2010-03-03 _Accession_date 2010-03-03 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Stauffer Melissa E. . 2 Chazin Walter J. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-03-24 original author . stop_ _Original_release_date 2010-03-24 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Physical Interaction between Replication Protein A and Rad51 Promotes Exchange on Single-stranded DNA' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15056657 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Stauffer Melissa E. . 2 Chazin Walter J. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 279 _Journal_issue 24 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 25638 _Page_last 25645 _Year 2004 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'RPA70A Rad51N complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label RPA70A $RPA70A Rad51N $Rad51N stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_RPA70A _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common RPA70A _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 252 _Mol_residue_sequence ; MSKVVPIASLTPYQSKWTIC ARVTNKSQIRTWSNSRGEGK LFSLELVDESGEIRATAFNE QVDKFFPLIEVNKVYYFSKG TLKIANKQFTAVKNDYEMTF NNETSVMPCEDDHHLPTVQF DFTGIDDLENKSKDSLVDII GICKSYEDATKITVRSNNRE VAKRNIYLMDTSGKVVTATL WGEDADKFDGSRQPVLAIKG ARVSDFGGRSLSVLSSSTII ANPDIPEAYKLRGWFDAEGQ ALDGVSISDLKS ; loop_ _Residue_seq_code _Residue_label 1 MET 2 SER 3 LYS 4 VAL 5 VAL 6 PRO 7 ILE 8 ALA 9 SER 10 LEU 11 THR 12 PRO 13 TYR 14 GLN 15 SER 16 LYS 17 TRP 18 THR 19 ILE 20 CYS 21 ALA 22 ARG 23 VAL 24 THR 25 ASN 26 LYS 27 SER 28 GLN 29 ILE 30 ARG 31 THR 32 TRP 33 SER 34 ASN 35 SER 36 ARG 37 GLY 38 GLU 39 GLY 40 LYS 41 LEU 42 PHE 43 SER 44 LEU 45 GLU 46 LEU 47 VAL 48 ASP 49 GLU 50 SER 51 GLY 52 GLU 53 ILE 54 ARG 55 ALA 56 THR 57 ALA 58 PHE 59 ASN 60 GLU 61 GLN 62 VAL 63 ASP 64 LYS 65 PHE 66 PHE 67 PRO 68 LEU 69 ILE 70 GLU 71 VAL 72 ASN 73 LYS 74 VAL 75 TYR 76 TYR 77 PHE 78 SER 79 LYS 80 GLY 81 THR 82 LEU 83 LYS 84 ILE 85 ALA 86 ASN 87 LYS 88 GLN 89 PHE 90 THR 91 ALA 92 VAL 93 LYS 94 ASN 95 ASP 96 TYR 97 GLU 98 MET 99 THR 100 PHE 101 ASN 102 ASN 103 GLU 104 THR 105 SER 106 VAL 107 MET 108 PRO 109 CYS 110 GLU 111 ASP 112 ASP 113 HIS 114 HIS 115 LEU 116 PRO 117 THR 118 VAL 119 GLN 120 PHE 121 ASP 122 PHE 123 THR 124 GLY 125 ILE 126 ASP 127 ASP 128 LEU 129 GLU 130 ASN 131 LYS 132 SER 133 LYS 134 ASP 135 SER 136 LEU 137 VAL 138 ASP 139 ILE 140 ILE 141 GLY 142 ILE 143 CYS 144 LYS 145 SER 146 TYR 147 GLU 148 ASP 149 ALA 150 THR 151 LYS 152 ILE 153 THR 154 VAL 155 ARG 156 SER 157 ASN 158 ASN 159 ARG 160 GLU 161 VAL 162 ALA 163 LYS 164 ARG 165 ASN 166 ILE 167 TYR 168 LEU 169 MET 170 ASP 171 THR 172 SER 173 GLY 174 LYS 175 VAL 176 VAL 177 THR 178 ALA 179 THR 180 LEU 181 TRP 182 GLY 183 GLU 184 ASP 185 ALA 186 ASP 187 LYS 188 PHE 189 ASP 190 GLY 191 SER 192 ARG 193 GLN 194 PRO 195 VAL 196 LEU 197 ALA 198 ILE 199 LYS 200 GLY 201 ALA 202 ARG 203 VAL 204 SER 205 ASP 206 PHE 207 GLY 208 GLY 209 ARG 210 SER 211 LEU 212 SER 213 VAL 214 LEU 215 SER 216 SER 217 SER 218 THR 219 ILE 220 ILE 221 ALA 222 ASN 223 PRO 224 ASP 225 ILE 226 PRO 227 GLU 228 ALA 229 TYR 230 LYS 231 LEU 232 ARG 233 GLY 234 TRP 235 PHE 236 ASP 237 ALA 238 GLU 239 GLY 240 GLN 241 ALA 242 LEU 243 ASP 244 GLY 245 VAL 246 SER 247 ILE 248 SER 249 ASP 250 LEU 251 LYS 252 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-05 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1FGU "Ssdna-Binding Domain Of The Large Subunit Of Replication Protein A" 100.00 252 100.00 100.00 0.00e+00 PDB 1JMC "Single Stranded Dna-Binding Domain Of Human Replication Protein A Bound To Single Stranded Dna, Rpa70 Subunit, Residues 183-420" 95.63 246 100.00 100.00 3.65e-177 DBJ BAD92969 "replication protein A1, 70kDa variant [Homo sapiens]" 99.60 630 99.60 99.60 9.68e-179 DBJ BAF82393 "unnamed protein product [Homo sapiens]" 99.60 616 100.00 100.00 1.22e-179 DBJ BAG10883 "replication protein A 70 kDa DNA-binding subunit [synthetic construct]" 99.60 616 99.60 99.60 6.25e-179 EMBL CAH92206 "hypothetical protein [Pongo abelii]" 99.60 616 99.60 99.60 2.99e-178 GB AAA36584 "replication protein A, 70-kDa subunit [Homo sapiens]" 99.60 616 100.00 100.00 1.22e-179 GB AAH18126 "Replication protein A1, 70kDa [Homo sapiens]" 99.60 616 100.00 100.00 1.22e-179 GB AAS94324 "replication protein A1, 70kDa [Homo sapiens]" 99.60 616 100.00 100.00 1.22e-179 GB ADR83314 "replication protein A1, 70kDa [synthetic construct]" 99.60 616 100.00 100.00 1.22e-179 GB AIC58576 "RPA1, partial [synthetic construct]" 99.60 616 100.00 100.00 1.75e-179 REF NP_001126290 "replication protein A 70 kDa DNA-binding subunit [Pongo abelii]" 99.60 616 99.60 99.60 2.99e-178 REF NP_002936 "replication protein A 70 kDa DNA-binding subunit [Homo sapiens]" 99.60 616 100.00 100.00 1.22e-179 REF XP_002747872 "PREDICTED: replication protein A 70 kDa DNA-binding subunit isoform X1 [Callithrix jacchus]" 99.60 616 97.61 99.20 8.10e-176 REF XP_002800290 "PREDICTED: replication protein A 70 kDa DNA-binding subunit-like, partial [Macaca mulatta]" 99.60 532 99.20 99.60 1.47e-178 REF XP_003277864 "PREDICTED: replication protein A 70 kDa DNA-binding subunit [Nomascus leucogenys]" 99.60 616 99.60 99.60 5.08e-179 SP P27694 "RecName: Full=Replication protein A 70 kDa DNA-binding subunit; Short=RP-A p70; AltName: Full=Replication factor A protein 1; S" 99.60 616 100.00 100.00 1.22e-179 SP Q5R7Q4 "RecName: Full=Replication protein A 70 kDa DNA-binding subunit; Short=RP-A p70; AltName: Full=Replication factor A protein 1; S" 99.60 616 99.60 99.60 2.99e-178 stop_ save_ save_Rad51N _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Rad51N _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 114 _Mol_residue_sequence ; MAMQMQLEANADTSVEEESF GPQPISRLEQCGINANDVKK LEEAGFHTVEAVAYAPKKEL INIKGISEAKADKILAEAAK LVPMGFTTATEFHQRRSEII QITTGSKELDKLLQ ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ALA 3 MET 4 GLN 5 MET 6 GLN 7 LEU 8 GLU 9 ALA 10 ASN 11 ALA 12 ASP 13 THR 14 SER 15 VAL 16 GLU 17 GLU 18 GLU 19 SER 20 PHE 21 GLY 22 PRO 23 GLN 24 PRO 25 ILE 26 SER 27 ARG 28 LEU 29 GLU 30 GLN 31 CYS 32 GLY 33 ILE 34 ASN 35 ALA 36 ASN 37 ASP 38 VAL 39 LYS 40 LYS 41 LEU 42 GLU 43 GLU 44 ALA 45 GLY 46 PHE 47 HIS 48 THR 49 VAL 50 GLU 51 ALA 52 VAL 53 ALA 54 TYR 55 ALA 56 PRO 57 LYS 58 LYS 59 GLU 60 LEU 61 ILE 62 ASN 63 ILE 64 LYS 65 GLY 66 ILE 67 SER 68 GLU 69 ALA 70 LYS 71 ALA 72 ASP 73 LYS 74 ILE 75 LEU 76 ALA 77 GLU 78 ALA 79 ALA 80 LYS 81 LEU 82 VAL 83 PRO 84 MET 85 GLY 86 PHE 87 THR 88 THR 89 ALA 90 THR 91 GLU 92 PHE 93 HIS 94 GLN 95 ARG 96 ARG 97 SER 98 GLU 99 ILE 100 ILE 101 GLN 102 ILE 103 THR 104 THR 105 GLY 106 SER 107 LYS 108 GLU 109 LEU 110 ASP 111 LYS 112 LEU 113 LEU 114 GLN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 11046 hsRad51 100.00 339 100.00 100.00 3.38e-73 BMRB 4328 HsRad51(1-114) 100.00 114 100.00 100.00 4.24e-76 PDB 1B22 "Rad51 (N-Terminal Domain)" 100.00 114 100.00 100.00 4.24e-76 DBJ BAA02718 "Rad51 protein [Mus musculus]" 100.00 339 97.37 99.12 2.41e-71 DBJ BAA02961 "MmRad51 [Mus musculus]" 100.00 339 97.37 99.12 2.41e-71 DBJ BAA02962 "HsRad51 [Homo sapiens]" 100.00 339 100.00 100.00 3.34e-73 DBJ BAA03189 "RAD51 [Homo sapiens]" 100.00 339 100.00 100.00 3.38e-73 DBJ BAB27489 "unnamed protein product [Mus musculus]" 100.00 339 97.37 99.12 2.41e-71 EMBL CAA69384 "rad51 [Cricetulus griseus]" 100.00 339 99.12 100.00 2.36e-72 EMBL CAG38796 "RAD51 [Homo sapiens]" 100.00 339 100.00 100.00 3.38e-73 GB AAC28561 "Rad51 [Oryctolagus cuniculus]" 100.00 339 97.37 98.25 5.24e-71 GB AAD49705 "Rad51 [Homo sapiens]" 100.00 339 100.00 100.00 3.38e-73 GB AAF61901 "RAD51 [Homo sapiens]" 65.79 75 100.00 100.00 8.76e-46 GB AAF69145 "RAD51 [Homo sapiens]" 100.00 339 100.00 100.00 3.38e-73 GB AAH27384 "RAD51 homolog (S. cerevisiae) [Mus musculus]" 100.00 339 97.37 99.12 2.41e-71 PRF 2102359A "RAD51-like protein" 100.00 339 97.37 99.12 2.41e-71 REF NP_001003043 "DNA repair protein RAD51 homolog 1 [Canis lupus familiaris]" 100.00 339 98.25 99.12 1.12e-71 REF NP_001039644 "DNA repair protein RAD51 homolog 1 [Bos taurus]" 100.00 339 99.12 99.12 1.60e-72 REF NP_001075493 "DNA repair protein RAD51 homolog 1 [Oryctolagus cuniculus]" 100.00 339 97.37 98.25 5.24e-71 REF NP_001102674 "DNA repair protein RAD51 homolog 1 [Rattus norvegicus]" 100.00 339 99.12 100.00 1.07e-72 REF NP_001116653 "DNA repair protein RAD51 homolog 1 [Sus scrofa]" 100.00 339 97.37 99.12 2.31e-71 SP O77507 "RecName: Full=DNA repair protein RAD51 homolog 1 [Oryctolagus cuniculus]" 100.00 339 97.37 98.25 5.24e-71 SP P70099 "RecName: Full=DNA repair protein RAD51 homolog 1 [Cricetulus griseus]" 100.00 339 99.12 100.00 2.36e-72 SP Q06609 "RecName: Full=DNA repair protein RAD51 homolog 1; Short=HsRAD51; Short=hRAD51; AltName: Full=RAD51 homolog A [Homo sapiens]" 100.00 339 100.00 100.00 3.38e-73 SP Q08297 "RecName: Full=DNA repair protein RAD51 homolog 1; AltName: Full=RAD51 homolog A [Mus musculus]" 100.00 339 97.37 99.12 2.41e-71 SP Q2KJ94 "RecName: Full=DNA repair protein RAD51 homolog 1 [Bos taurus]" 100.00 339 99.12 99.12 1.60e-72 TPG DAA25435 "TPA: DNA repair protein RAD51 homolog 1 [Bos taurus]" 100.00 339 99.12 99.12 1.60e-72 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $RPA70A Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $RPA70A 'recombinant technology' . Escherichia coli . pET15b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $Rad51N . mM 0.3 0.8 [U-15N] $RPA70A . mM 0.3 0.8 [U-15N] Tis 10 mM . . 'natural abundance' 'Potassium Chloride' 20 mM . . 'natural abundance' BME 1 mM . . 'natural abundance' 'sodium azide' 1 uM . . 'natural abundance' H2O 100 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_Felix _Saveframe_category software _Name FELIX _Version . loop_ _Vendor _Address _Electronic_address 'Accelrys Software Inc.' . . stop_ loop_ _Task processing stop_ _Details . save_ save_HADDOCK _Saveframe_category software _Name HADDOCK _Version . loop_ _Vendor _Address _Electronic_address 'Dominguez, C., Boelens, R., and Bonvin, A.M. 2003' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.0 . pH pressure 1 . atm temperature 300 . K stop_ save_