data_16760 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Fibrinopeptide A binds Gly-Pro-Arg-Pro ; _BMRB_accession_number 16760 _BMRB_flat_file_name bmr16760.str _Entry_type new _Submission_date 2010-03-03 _Accession_date 2010-03-03 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Root-Bernstein Robert S. . 2 Westall Fred C. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-03-24 original author . stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Fibrinopeptide A binds Gly-Pro-Arg-Pro' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 6589598 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Root-Bernstein Robert S. . 2 Westall Fred C. . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_volume 81 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4339 _Page_last 4342 _Year 1984 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'FPA/Gly-Pro-Arg-Pro complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label FPA $FPA Gly-Pro-Arg-Pro $GlyProArgPro stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_FPA _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common FPA _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 16 _Mol_residue_sequence ADSGEGDFLAEGGGVR loop_ _Residue_seq_code _Residue_label 1 ALA 2 ASP 3 SER 4 GLY 5 GLU 6 GLY 7 ASP 8 PHE 9 LEU 10 ALA 11 GLU 12 GLY 13 GLY 14 GLY 15 VAL 16 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 3H32 "Crystal Structure Of D-Dimer From Human Fibrin Complexed With Gly-His- Arg-Pro-Tyr-Amide" 100.00 197 100.00 100.00 1.61e-01 GB AAH20764 "FGA protein [Homo sapiens]" 100.00 218 100.00 100.00 9.63e-02 GB AAH70246 "FGA protein [Homo sapiens]" 100.00 289 100.00 100.00 1.29e-01 GB AAI05804 "FGA protein, partial [Homo sapiens]" 100.00 250 100.00 100.00 1.12e-01 GB ADR82694 "Unknown protein [synthetic construct]" 100.00 218 100.00 100.00 9.63e-02 GB AIC58950 "FGA, partial [synthetic construct]" 100.00 218 100.00 100.00 9.63e-02 PRF 650770B "fibrinopeptide A" 100.00 16 100.00 100.00 2.81e+00 PRF 701211A "fibrinopeptide A" 100.00 16 100.00 100.00 2.81e+00 stop_ save_ save_GlyProArgPro _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common GlyProArgPro _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 4 _Mol_residue_sequence GPRP loop_ _Residue_seq_code _Residue_label 1 GLY 2 PRO 3 ARG 4 PRO stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $FPA Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $FPA 'obtained from a vendor' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details ; NaOH and HCl were used in minute amounts to bring the pH to 7; 0.05-0.10 ml aliquots of Gly-Pro-Arg-Pro were added progressively to the FPA solution; negligible amounts of HCl were used to adjust the pH ; loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $FPA 5 mg . . 'natural abundance' $GlyProArgPro . ml 0.05 0.10 'natural abundance' D2O 100 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software _Saveframe_category software _Name not_given _Version . loop_ _Vendor _Address _Electronic_address 'not given' . . stop_ loop_ _Task 'not given' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model WM _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_1H_NMR_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1H NMR' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298 . K pH 7.0 . pH pressure 1 . atm stop_ save_