data_16762 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Mapping the Ca2+-dependent binding of an invertebrate homolog of protein phosphatase 4 regulatory subunit 2 of the small EF-hand protein, calsensin ; _BMRB_accession_number 16762 _BMRB_flat_file_name bmr16762.str _Entry_type original _Submission_date 2010-03-03 _Accession_date 2010-03-03 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Venkitaramani Deepa V. . 2 Fulton D. B. . 3 Andreotti Amy H. . 4 Johansen Kristen M. . 5 Johansen Jorgen . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-03-24 original author . stop_ _Original_release_date 2010-03-24 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Mapping the Ca2+-dependent binding of an invertebrate homolog of protein phosphatase 4 regulatory subunit 2 of the small EF-hand protein, calsensin' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16600403 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Venkitaramani Deepa V. . 2 Fulton D. B. . 3 Andreotti Amy H. . 4 Johansen Kristen M. . 5 Johansen Jorgen . . stop_ _Journal_abbreviation 'Biochim. Biophys. Acta' _Journal_volume 1763 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 322 _Page_last 329 _Year 2006 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'PP4R2/Calsensin complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label PP4R2 $PP4R2 Calsensin $Calsensin stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_PP4R2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common PP4R2 _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 423 _Mol_residue_sequence ; MGSRKEIRDALEEFLKQPED GEVNGILERYLSRVAVTGIT LYPWSKLKPLITRKMNLVVE EFMKECPMESLALNKNVENT SVEDMKKRLNDSIELFNGIP FTIQRLCELLTDQNISKQYR RVDKFMRGIEKNLNVVTTVD PFGNRIVGDPKSASNGVLSA EDDCLLTPTSCTTVKTPPSW FSSLSSVVNSPDSYPNWKQS QDEQQQQEQRDAAISSSRER KKPRSEDDAVDKDCLPPPSK LFRPGDEFVDEAKQPETKDG SDSSVATNSTFTPLLTQLLN GSTPISSSEKEQSVVGSCDS TESSQPSGLLEILTGKLKDP ETNLDASEGTPSVDVGAVGT TSQTDASEVFGAVKSEATQS TQYHVEITEHANKKDVSTAS DKKTDEKIQQEPAEVKRTQE GELESSGVKCQQPSDDPDSL FSF ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLY 3 SER 4 ARG 5 LYS 6 GLU 7 ILE 8 ARG 9 ASP 10 ALA 11 LEU 12 GLU 13 GLU 14 PHE 15 LEU 16 LYS 17 GLN 18 PRO 19 GLU 20 ASP 21 GLY 22 GLU 23 VAL 24 ASN 25 GLY 26 ILE 27 LEU 28 GLU 29 ARG 30 TYR 31 LEU 32 SER 33 ARG 34 VAL 35 ALA 36 VAL 37 THR 38 GLY 39 ILE 40 THR 41 LEU 42 TYR 43 PRO 44 TRP 45 SER 46 LYS 47 LEU 48 LYS 49 PRO 50 LEU 51 ILE 52 THR 53 ARG 54 LYS 55 MET 56 ASN 57 LEU 58 VAL 59 VAL 60 GLU 61 GLU 62 PHE 63 MET 64 LYS 65 GLU 66 CYS 67 PRO 68 MET 69 GLU 70 SER 71 LEU 72 ALA 73 LEU 74 ASN 75 LYS 76 ASN 77 VAL 78 GLU 79 ASN 80 THR 81 SER 82 VAL 83 GLU 84 ASP 85 MET 86 LYS 87 LYS 88 ARG 89 LEU 90 ASN 91 ASP 92 SER 93 ILE 94 GLU 95 LEU 96 PHE 97 ASN 98 GLY 99 ILE 100 PRO 101 PHE 102 THR 103 ILE 104 GLN 105 ARG 106 LEU 107 CYS 108 GLU 109 LEU 110 LEU 111 THR 112 ASP 113 GLN 114 ASN 115 ILE 116 SER 117 LYS 118 GLN 119 TYR 120 ARG 121 ARG 122 VAL 123 ASP 124 LYS 125 PHE 126 MET 127 ARG 128 GLY 129 ILE 130 GLU 131 LYS 132 ASN 133 LEU 134 ASN 135 VAL 136 VAL 137 THR 138 THR 139 VAL 140 ASP 141 PRO 142 PHE 143 GLY 144 ASN 145 ARG 146 ILE 147 VAL 148 GLY 149 ASP 150 PRO 151 LYS 152 SER 153 ALA 154 SER 155 ASN 156 GLY 157 VAL 158 LEU 159 SER 160 ALA 161 GLU 162 ASP 163 ASP 164 CYS 165 LEU 166 LEU 167 THR 168 PRO 169 THR 170 SER 171 CYS 172 THR 173 THR 174 VAL 175 LYS 176 THR 177 PRO 178 PRO 179 SER 180 TRP 181 PHE 182 SER 183 SER 184 LEU 185 SER 186 SER 187 VAL 188 VAL 189 ASN 190 SER 191 PRO 192 ASP 193 SER 194 TYR 195 PRO 196 ASN 197 TRP 198 LYS 199 GLN 200 SER 201 GLN 202 ASP 203 GLU 204 GLN 205 GLN 206 GLN 207 GLN 208 GLU 209 GLN 210 ARG 211 ASP 212 ALA 213 ALA 214 ILE 215 SER 216 SER 217 SER 218 ARG 219 GLU 220 ARG 221 LYS 222 LYS 223 PRO 224 ARG 225 SER 226 GLU 227 ASP 228 ASP 229 ALA 230 VAL 231 ASP 232 LYS 233 ASP 234 CYS 235 LEU 236 PRO 237 PRO 238 PRO 239 SER 240 LYS 241 LEU 242 PHE 243 ARG 244 PRO 245 GLY 246 ASP 247 GLU 248 PHE 249 VAL 250 ASP 251 GLU 252 ALA 253 LYS 254 GLN 255 PRO 256 GLU 257 THR 258 LYS 259 ASP 260 GLY 261 SER 262 ASP 263 SER 264 SER 265 VAL 266 ALA 267 THR 268 ASN 269 SER 270 THR 271 PHE 272 THR 273 PRO 274 LEU 275 LEU 276 THR 277 GLN 278 LEU 279 LEU 280 ASN 281 GLY 282 SER 283 THR 284 PRO 285 ILE 286 SER 287 SER 288 SER 289 GLU 290 LYS 291 GLU 292 GLN 293 SER 294 VAL 295 VAL 296 GLY 297 SER 298 CYS 299 ASP 300 SER 301 THR 302 GLU 303 SER 304 SER 305 GLN 306 PRO 307 SER 308 GLY 309 LEU 310 LEU 311 GLU 312 ILE 313 LEU 314 THR 315 GLY 316 LYS 317 LEU 318 LYS 319 ASP 320 PRO 321 GLU 322 THR 323 ASN 324 LEU 325 ASP 326 ALA 327 SER 328 GLU 329 GLY 330 THR 331 PRO 332 SER 333 VAL 334 ASP 335 VAL 336 GLY 337 ALA 338 VAL 339 GLY 340 THR 341 THR 342 SER 343 GLN 344 THR 345 ASP 346 ALA 347 SER 348 GLU 349 VAL 350 PHE 351 GLY 352 ALA 353 VAL 354 LYS 355 SER 356 GLU 357 ALA 358 THR 359 GLN 360 SER 361 THR 362 GLN 363 TYR 364 HIS 365 VAL 366 GLU 367 ILE 368 THR 369 GLU 370 HIS 371 ALA 372 ASN 373 LYS 374 LYS 375 ASP 376 VAL 377 SER 378 THR 379 ALA 380 SER 381 ASP 382 LYS 383 LYS 384 THR 385 ASP 386 GLU 387 LYS 388 ILE 389 GLN 390 GLN 391 GLU 392 PRO 393 ALA 394 GLU 395 VAL 396 LYS 397 ARG 398 THR 399 GLN 400 GLU 401 GLY 402 GLU 403 LEU 404 GLU 405 SER 406 SER 407 GLY 408 VAL 409 LYS 410 CYS 411 GLN 412 GLN 413 PRO 414 SER 415 ASP 416 ASP 417 PRO 418 ASP 419 SER 420 LEU 421 PHE 422 SER 423 PHE stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2013-12-02 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value GB ABC88653 "protein phosphatase 4 regulatory subunit 2 [Haemopis marmorata]" 100.00 423 100.00 100.00 0.00e+00 stop_ save_ save_Calsensin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Calsensin _Molecular_mass . _Mol_thiol_state 'not reported' _Details . _Residue_count 83 _Mol_residue_sequence ; MACKVKAELEAAFKKLDANG DGYVTALELQTFMVTLDAYK ALSKDKVKEASAKLIKMADK NSDGKISKEEFLNANAELLC QLK ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ALA 3 CYS 4 LYS 5 VAL 6 LYS 7 ALA 8 GLU 9 LEU 10 GLU 11 ALA 12 ALA 13 PHE 14 LYS 15 LYS 16 LEU 17 ASP 18 ALA 19 ASN 20 GLY 21 ASP 22 GLY 23 TYR 24 VAL 25 THR 26 ALA 27 LEU 28 GLU 29 LEU 30 GLN 31 THR 32 PHE 33 MET 34 VAL 35 THR 36 LEU 37 ASP 38 ALA 39 TYR 40 LYS 41 ALA 42 LEU 43 SER 44 LYS 45 ASP 46 LYS 47 VAL 48 LYS 49 GLU 50 ALA 51 SER 52 ALA 53 LYS 54 LEU 55 ILE 56 LYS 57 MET 58 ALA 59 ASP 60 LYS 61 ASN 62 SER 63 ASP 64 GLY 65 LYS 66 ILE 67 SER 68 LYS 69 GLU 70 GLU 71 PHE 72 LEU 73 ASN 74 ALA 75 ASN 76 ALA 77 GLU 78 LEU 79 LEU 80 CYS 81 GLN 82 LEU 83 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-10-19 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 6519 Calsensin 100.00 83 100.00 100.00 8.57e-49 PDB 1YX7 "Nmr Structure Of Calsensin, Energy Minimized Average Structure." 100.00 83 100.00 100.00 8.57e-49 PDB 1YX8 "Nmr Structure Of Calsensin, 20 Low Energy Structures." 100.00 83 100.00 100.00 8.57e-49 GB AAC46630 "calsensin [Haemopis marmorata]" 100.00 83 100.00 100.00 8.57e-49 SP Q25088 "RecName: Full=Calsensin; AltName: Full=LAN3-6 antigen [Haemopis marmorata]" 100.00 83 100.00 100.00 8.57e-49 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $PP4R2 'segemented worms' 38567 Eukaryota Metazoa Hirudo marmorata stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $PP4R2 'recombinant technology' . Escherichia coli BL21 pGEX4T3 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'PP4R2 was titrated into solution' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $Calsensin 0.8 mM . . '[U-100% 15N]' 'sodium phosphate' 50 mM . . 'natural abundance' 'sodium chloride' 75 mM . . 'natural abundance' DTT 2 mM . . 'natural abundance' EDTA 8 mM . . 'natural abundance' 'sodium azide' 0.02 % . . 'natural abundance' $PP4R2 . mM 0 0.9 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task assignments stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details 'Operated at 499.867 MHz' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.0 . pH pressure 1 . atm temperature 298 . K stop_ save_