data_17060 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Replication protein A 32 interacts through a similar binding interface with TIPIN, XPA, and UNG2 ; _BMRB_accession_number 17060 _BMRB_flat_file_name bmr17060.str _Entry_type new _Submission_date 2010-07-13 _Accession_date 2010-07-13 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ali Seikh I. . 2 Shin Jae-Sun . . 3 Bae Sung-Hun . . 4 Kim Byoungkook . . 5 Choi Byong-Seok . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-09-08 original author . stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Replication protein A 32 interacts through a similar binding interface with TIPIN, XPA, and UNG2' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ali Seikh I. . 2 Shin Jae-Sun . . 3 Bae Sung-Hun . . 4 Kim Byoungkook . . 5 Choi Byong-Seok . . stop_ _Journal_abbreviation 'INT. J. BIOCHEM. CELL B' _Journal_volume 42 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1210 _Page_last 1215 _Year 2010 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name RPA32C/TIPIN _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label RPA32C $RPA32C TIPIN $TIPIN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_RPA32C _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common RPA32C _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 97 _Mol_residue_sequence ; SQPSAGRAPISNPGMSEAGN FGGNSFMPANGLTVAQNQVL NLIKACPRPEGLNFQDLKNQ LKHMSVSSIKQAVDFLSNEG HIYSTVDDDHFKSTDAE ; loop_ _Residue_seq_code _Residue_label 1 SER 2 GLN 3 PRO 4 SER 5 ALA 6 GLY 7 ARG 8 ALA 9 PRO 10 ILE 11 SER 12 ASN 13 PRO 14 GLY 15 MET 16 SER 17 GLU 18 ALA 19 GLY 20 ASN 21 PHE 22 GLY 23 GLY 24 ASN 25 SER 26 PHE 27 MET 28 PRO 29 ALA 30 ASN 31 GLY 32 LEU 33 THR 34 VAL 35 ALA 36 GLN 37 ASN 38 GLN 39 VAL 40 LEU 41 ASN 42 LEU 43 ILE 44 LYS 45 ALA 46 CYS 47 PRO 48 ARG 49 PRO 50 GLU 51 GLY 52 LEU 53 ASN 54 PHE 55 GLN 56 ASP 57 LEU 58 LYS 59 ASN 60 GLN 61 LEU 62 LYS 63 HIS 64 MET 65 SER 66 VAL 67 SER 68 SER 69 ILE 70 LYS 71 GLN 72 ALA 73 VAL 74 ASP 75 PHE 76 LEU 77 SER 78 ASN 79 GLU 80 GLY 81 HIS 82 ILE 83 TYR 84 SER 85 THR 86 VAL 87 ASP 88 ASP 89 ASP 90 HIS 91 PHE 92 LYS 93 SER 94 THR 95 ASP 96 ALA 97 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-05 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 4460 "Replication Protein A" 100.00 103 100.00 100.00 2.79e-64 PDB 1DPU "Solution Structure Of The C-Terminal Domain Of Human Rpa32 Complexed With Ung2(73-88)" 100.00 99 100.00 100.00 2.61e-64 PDB 1Z1D "Structural Model For The Interaction Between Rpa32 C- Terminal Domain And Sv40 T Antigen Origin Binding Domain" 100.00 103 100.00 100.00 2.79e-64 PDB 2PI2 "Full-Length Replication Protein A Subunits Rpa14 And Rpa32" 100.00 270 100.00 100.00 6.85e-63 PDB 2Z6K "Crystal Structure Of Full-Length Human Rpa1432 HETERODIMER" 100.00 270 100.00 100.00 6.85e-63 PDB 4MQV "Crystal Complex Of Rpa32c And Smarcal1 N-terminus" 71.13 69 100.00 100.00 2.66e-42 PDB 4OU0 "Crystal Structure Of Rpa32c" 71.13 73 98.55 98.55 1.70e-40 DBJ BAG35795 "unnamed protein product [Homo sapiens]" 100.00 270 98.97 100.00 2.35e-62 DBJ BAG59456 "unnamed protein product [Homo sapiens]" 100.00 104 100.00 100.00 1.84e-64 DBJ BAG60901 "unnamed protein product [Homo sapiens]" 100.00 264 100.00 100.00 5.40e-63 DBJ BAG62374 "unnamed protein product [Homo sapiens]" 100.00 174 100.00 100.00 3.50e-64 DBJ BAI47325 "replication protein A2, 32kDa [synthetic construct]" 100.00 270 100.00 100.00 6.85e-63 EMBL CAG29344 "RPA2 [Homo sapiens]" 100.00 270 100.00 100.00 6.85e-63 EMBL CAH90667 "hypothetical protein [Pongo abelii]" 100.00 270 97.94 100.00 4.22e-62 GB AAA36560 "replication protein A [Homo sapiens]" 100.00 270 100.00 100.00 6.85e-63 GB AAH01630 "Replication protein A2, 32kDa [Homo sapiens]" 100.00 270 100.00 100.00 6.85e-63 GB AAH12157 "Replication protein A2, 32kDa [Homo sapiens]" 100.00 270 100.00 100.00 6.85e-63 GB AAH21257 "Replication protein A2, 32kDa [Homo sapiens]" 100.00 270 100.00 100.00 6.85e-63 GB AAX84514 "replication protein A2, 32kDa [Homo sapiens]" 100.00 270 100.00 100.00 6.85e-63 REF NP_001125362 "replication protein A 32 kDa subunit [Pongo abelii]" 100.00 343 97.94 100.00 1.43e-61 REF NP_001247563 "replication protein A 32 kDa subunit [Macaca mulatta]" 100.00 270 98.97 100.00 2.59e-62 REF NP_001273005 "replication protein A 32 kDa subunit isoform 2 [Homo sapiens]" 100.00 174 100.00 100.00 3.50e-64 REF NP_001284487 "replication protein A 32 kDa subunit isoform 3 [Homo sapiens]" 100.00 278 100.00 100.00 8.26e-63 REF NP_002937 "replication protein A 32 kDa subunit isoform 1 [Homo sapiens]" 100.00 270 100.00 100.00 6.85e-63 SP P15927 "RecName: Full=Replication protein A 32 kDa subunit; Short=RP-A p32; AltName: Full=Replication factor A protein 2; Short=RF-A pr" 100.00 270 100.00 100.00 6.85e-63 SP Q5RC43 "RecName: Full=Replication protein A 32 kDa subunit; Short=RP-A p32; AltName: Full=Replication factor A protein 2; Short=RF-A pr" 100.00 270 97.94 100.00 4.22e-62 stop_ save_ save_TIPIN _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common TIPIN _Molecular_mass . _Mol_thiol_state 'not reported' _Details . _Residue_count 34 _Mol_residue_sequence ; EMFASELSRSLTEEQQRRIE RNKQLALERRQAKL ; loop_ _Residue_seq_code _Residue_label 1 GLU 2 MET 3 PHE 4 ALA 5 SER 6 GLU 7 LEU 8 SER 9 ARG 10 SER 11 LEU 12 THR 13 GLU 14 GLU 15 GLN 16 GLN 17 ARG 18 ARG 19 ILE 20 GLU 21 ARG 22 ASN 23 LYS 24 GLN 25 LEU 26 ALA 27 LEU 28 GLU 29 ARG 30 ARG 31 GLN 32 ALA 33 LYS 34 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAA91229 "unnamed protein product [Homo sapiens]" 100.00 301 100.00 100.00 1.37e-11 DBJ BAG61265 "unnamed protein product [Homo sapiens]" 100.00 200 97.06 100.00 4.05e-11 DBJ BAI46472 "TIMELESS interacting protein [synthetic construct]" 100.00 301 97.06 100.00 7.22e-11 EMBL CAL38286 "hypothetical protein [synthetic construct]" 100.00 301 97.06 100.00 7.22e-11 GB AAH00870 "TIMELESS interacting protein [Homo sapiens]" 100.00 301 97.06 100.00 7.22e-11 GB ACB21044 "TIMELESS interacting protein [Homo sapiens]" 100.00 301 97.06 100.00 7.69e-11 GB AIC51688 "TIPIN, partial [synthetic construct]" 100.00 301 97.06 100.00 7.22e-11 GB EAW77762 "timeless-interacting protein, isoform CRA_a [Homo sapiens]" 100.00 301 97.06 100.00 7.22e-11 REF NP_001276915 "TIMELESS-interacting protein isoform 2 [Homo sapiens]" 100.00 200 97.06 100.00 4.05e-11 REF NP_060328 "TIMELESS-interacting protein isoform 1 [Homo sapiens]" 100.00 301 97.06 100.00 7.22e-11 REF XP_005254578 "PREDICTED: TIMELESS-interacting protein isoform X1 [Homo sapiens]" 100.00 301 97.06 100.00 7.69e-11 REF XP_005254579 "PREDICTED: TIMELESS-interacting protein isoform X2 [Homo sapiens]" 100.00 301 97.06 100.00 7.69e-11 REF XP_006720656 "PREDICTED: TIMELESS-interacting protein isoform X4 [Homo sapiens]" 100.00 301 97.06 100.00 7.69e-11 SP Q9BVW5 "RecName: Full=TIMELESS-interacting protein [Homo sapiens]" 100.00 301 97.06 100.00 7.69e-11 TPG DAA01365 "TPA_exp: timeless-interacting protein [Homo sapiens]" 100.00 301 97.06 100.00 7.22e-11 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $RPA32C Human 9606 Eukaryota Metazoa . . $TIPIN . . . . . . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $RPA32C 'recombinant technology' . Escherichia coli . pET15b $TIPIN 'recombinant technology' . Escherichia coli . pGEX4T1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'TIPIN was titrated into solution' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling 'sodium phosphate' 25 mM . . 'natural abundance' 'sodium chloride' 50 mM . . 'natural abundance' DTT 3 mM . . 'natural abundance' $RPA32C 0.1 mM . . [U-15N] $TIPIN . mM 0.02 3.0 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model 'not given' _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'not given' _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 293 . K pH 7.0 . pH pressure 1 . atm stop_ save_