data_17114 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy ; _BMRB_accession_number 17114 _BMRB_flat_file_name bmr17114.str _Entry_type new _Submission_date 2010-08-11 _Accession_date 2010-08-11 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hansen D. F. . 2 Zhou Zheng . . 3 Feng Haniqiao . . 4 Jenkins Lisa M.M. . 5 Bai Yawen . . 6 Kay Lewis E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count kinetic_rates 1 binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-11-10 original author . stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 19385041 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hansen D. F. . 2 Zhou Zheng . . 3 Feng Haniqiao . . 4 Jenkins Lisa M.M. . 5 Bai Yawen . . 6 Kay Lewis E. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_name_full 'Journal of Molecular Biology' _Journal_volume 387 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1 _Page_last 9 _Year 2009 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'H2AZH2B/Chz1 complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label H2AZH2B $H2AZH2B Chz1 $Chz1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_H2AZH2B _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common H2AZH2B _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 192 _Mol_residue_sequence ; RKETYSSYIYKVLKQTHPDT GISQKSMSILNSFVNDIFER IATEASKLAAYNKKSTISAR EIQTAVRLILPGELAKHAVS EGTRAVTKYSSSTQAQSSSA RAGLQFPVGRIKRYLKRHAT GRTRVGSKAAIYLTAVLEYL TAEVLELAGNAAKDLKVKRI TPRHLQLAIRGDDELDSLIR ATIASGGVLPHI ; loop_ _Residue_seq_code _Residue_label 1 ARG 2 LYS 3 GLU 4 THR 5 TYR 6 SER 7 SER 8 TYR 9 ILE 10 TYR 11 LYS 12 VAL 13 LEU 14 LYS 15 GLN 16 THR 17 HIS 18 PRO 19 ASP 20 THR 21 GLY 22 ILE 23 SER 24 GLN 25 LYS 26 SER 27 MET 28 SER 29 ILE 30 LEU 31 ASN 32 SER 33 PHE 34 VAL 35 ASN 36 ASP 37 ILE 38 PHE 39 GLU 40 ARG 41 ILE 42 ALA 43 THR 44 GLU 45 ALA 46 SER 47 LYS 48 LEU 49 ALA 50 ALA 51 TYR 52 ASN 53 LYS 54 LYS 55 SER 56 THR 57 ILE 58 SER 59 ALA 60 ARG 61 GLU 62 ILE 63 GLN 64 THR 65 ALA 66 VAL 67 ARG 68 LEU 69 ILE 70 LEU 71 PRO 72 GLY 73 GLU 74 LEU 75 ALA 76 LYS 77 HIS 78 ALA 79 VAL 80 SER 81 GLU 82 GLY 83 THR 84 ARG 85 ALA 86 VAL 87 THR 88 LYS 89 TYR 90 SER 91 SER 92 SER 93 THR 94 GLN 95 ALA 96 GLN 97 SER 98 SER 99 SER 100 ALA 101 ARG 102 ALA 103 GLY 104 LEU 105 GLN 106 PHE 107 PRO 108 VAL 109 GLY 110 ARG 111 ILE 112 LYS 113 ARG 114 TYR 115 LEU 116 LYS 117 ARG 118 HIS 119 ALA 120 THR 121 GLY 122 ARG 123 THR 124 ARG 125 VAL 126 GLY 127 SER 128 LYS 129 ALA 130 ALA 131 ILE 132 TYR 133 LEU 134 THR 135 ALA 136 VAL 137 LEU 138 GLU 139 TYR 140 LEU 141 THR 142 ALA 143 GLU 144 VAL 145 LEU 146 GLU 147 LEU 148 ALA 149 GLY 150 ASN 151 ALA 152 ALA 153 LYS 154 ASP 155 LEU 156 LYS 157 VAL 158 LYS 159 ARG 160 ILE 161 THR 162 PRO 163 ARG 164 HIS 165 LEU 166 GLN 167 LEU 168 ALA 169 ILE 170 ARG 171 GLY 172 ASP 173 ASP 174 GLU 175 LEU 176 ASP 177 SER 178 LEU 179 ILE 180 ARG 181 ALA 182 THR 183 ILE 184 ALA 185 SER 186 GLY 187 GLY 188 VAL 189 LEU 190 PRO 191 HIS 192 ILE stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-03-29 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15393 H2A.Z-H2B 100.00 200 100.00 100.00 1.04e-133 PDB 2JSS "Nmr Structure Of Chaperone Chz1 Complexed With Histone H2a.Z-H2b" 100.00 192 100.00 100.00 1.35e-133 PDB 4M6B "Crystal Structure Of Yeast Swr1-z Domain In Complex With H2a.z-h2b Dimer" 100.00 193 100.00 100.00 1.09e-133 EMBL CAY78724 "Htb1p [Saccharomyces cerevisiae EC1118]" 50.00 132 100.00 100.00 5.65e-60 GB AJV19600 "Htb1p [Saccharomyces cerevisiae YJM1574]" 50.00 132 100.00 100.00 5.65e-60 stop_ save_ save_Chz1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Chz1 _Molecular_mass . _Mol_thiol_state 'not reported' _Details . _Residue_count 62 _Mol_residue_sequence ; TVEDSESDMDDAKLDALMGN EGEEEEDDLAEIDTSNIITS GRRTRGKVIDYKKTAEELDK KE ; loop_ _Residue_seq_code _Residue_label 1 THR 2 VAL 3 GLU 4 ASP 5 SER 6 GLU 7 SER 8 ASP 9 MET 10 ASP 11 ASP 12 ALA 13 LYS 14 LEU 15 ASP 16 ALA 17 LEU 18 MET 19 GLY 20 ASN 21 GLU 22 GLY 23 GLU 24 GLU 25 GLU 26 GLU 27 ASP 28 ASP 29 LEU 30 ALA 31 GLU 32 ILE 33 ASP 34 THR 35 SER 36 ASN 37 ILE 38 ILE 39 THR 40 SER 41 GLY 42 ARG 43 ARG 44 THR 45 ARG 46 GLY 47 LYS 48 VAL 49 ILE 50 ASP 51 TYR 52 LYS 53 LYS 54 THR 55 ALA 56 GLU 57 GLU 58 LEU 59 ASP 60 LYS 61 LYS 62 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15393 Chz1 100.00 62 100.00 100.00 1.25e-32 PDB 2JSS "Nmr Structure Of Chaperone Chz1 Complexed With Histone H2a.Z-H2b" 100.00 62 100.00 100.00 1.25e-32 DBJ GAA22856 "K7_Chz1p [Saccharomyces cerevisiae Kyokai no. 7]" 100.00 147 100.00 100.00 2.48e-32 EMBL CAY79198 "Chz1p [Saccharomyces cerevisiae EC1118]" 100.00 160 100.00 100.00 2.61e-32 GB AAB64563 "Yer030wp [Saccharomyces cerevisiae]" 100.00 160 100.00 100.00 4.47e-32 GB AHY75583 "Chz1p [Saccharomyces cerevisiae YJM993]" 100.00 163 100.00 100.00 3.01e-32 GB EDN62999 "chaperone [Saccharomyces cerevisiae YJM789]" 100.00 167 100.00 100.00 3.41e-32 GB EDV08859 "chaperone [Saccharomyces cerevisiae RM11-1a]" 100.00 160 100.00 100.00 2.61e-32 GB EDZ72631 "YER030Wp-like protein [Saccharomyces cerevisiae AWRI1631]" 100.00 170 100.00 100.00 3.10e-32 REF NP_010947 "Chz1p [Saccharomyces cerevisiae S288c]" 100.00 153 100.00 100.00 3.00e-32 SP A6ZQX9 "RecName: Full=Histone H2A.Z-specific chaperone CHZ1 [Saccharomyces cerevisiae YJM789]" 100.00 160 100.00 100.00 2.61e-32 SP P40019 "RecName: Full=Histone H2A.Z-specific chaperone CHZ1 [Saccharomyces cerevisiae S288c]" 100.00 153 100.00 100.00 3.00e-32 TPG DAA07683 "TPA: Chz1p [Saccharomyces cerevisiae S288c]" 100.00 153 100.00 100.00 3.00e-32 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $H2AZH2B 'Baker's yeast' 4932 Eukaryota Fungi . . $Chz1 . . . . . . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $H2AZH2B 'recombinant technology' . . . . na $Chz1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $H2AZH2B 0.95 mM '[U-15N; U-2H]' $Chz1 1 mM '[U-15N; U-2H]' Mes 25 mM 'natural abundance' 'sodium chloride' 0.2 M 'natural abundance' EDTA 1 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_FuDA _Saveframe_category software _Name FuDA _Version . loop_ _Vendor _Address _Electronic_address 'flemming@pound.med.utoronto.ca; http://pound.med.utoronto.ca/software' . . stop_ loop_ _Task 'signal quantification' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 308 . K pH 6.0 . pH pressure ambient . atm stop_ save_