data_17119 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Defining the Intramembrane Binding Mechanism of Sarcolipin to Calcium ATPase USing Solution NMR Spectroscopy ; _BMRB_accession_number 17119 _BMRB_flat_file_name bmr17119.str _Entry_type original _Submission_date 2010-08-11 _Accession_date 2010-08-11 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Buffy Jarrod J. . 2 Buck-Koehntop Bethany A. . 3 Porcelli Fernando . . 4 Traaseth Nathaniel J. . 5 Veglia Gianluigi . . 6 Thomas David D. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-11-10 original author . stop_ _Original_release_date 2010-11-10 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Defining the Intramembrane Binding Mechanism of Sarcolipin to Calcium ATPase USing Solution NMR Spectroscopy' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16519897 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Buffy Jarrod J. . 2 Buck-Koehntop Bethany A. . 3 Porcelli Fernando . . 4 Traaseth Nathaniel J. . 5 Veglia Gianluigi . . 6 Thomas David D. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 358 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 420 _Page_last 429 _Year 2006 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'SLN/SERCA complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label SLN $SLN SERCA $SERCA stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_SLN _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common SLN _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 31 _Mol_residue_sequence ; MERSTRELCLNFTVVLITVI LIWLLVRSYQY ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLU 3 ARG 4 SER 5 THR 6 ARG 7 GLU 8 LEU 9 CYS 10 LEU 11 ASN 12 PHE 13 THR 14 VAL 15 VAL 16 LEU 17 ILE 18 THR 19 VAL 20 ILE 21 LEU 22 ILE 23 TRP 24 LEU 25 LEU 26 VAL 27 ARG 28 SER 29 TYR 30 GLN 31 TYR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-15 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 3W5A "Crystal Structure Of The Calcium Pump And Sarcolipin From Rabbit Fast Twitch Skeletal Muscle In The E1.mg2+ State" 100.00 31 100.00 100.00 2.86e-11 PDB 4H1W "E1 Structure Of The (sr) Ca2+-atpase In Complex With Sarcolipin" 100.00 31 100.00 100.00 2.86e-11 GB AAB86979 "sarcolipin [Oryctolagus cuniculus]" 100.00 31 100.00 100.00 2.86e-11 GB ABD98462 "sarcolipin [Sus scrofa]" 100.00 31 100.00 100.00 2.86e-11 REF NP_001038031 "sarcolipin [Sus scrofa]" 100.00 31 100.00 100.00 2.86e-11 REF NP_001075856 "sarcolipin [Oryctolagus cuniculus]" 100.00 31 100.00 100.00 2.86e-11 REF NP_001193294 "sarcolipin [Bos taurus]" 100.00 31 100.00 100.00 2.86e-11 REF XP_004320742 "PREDICTED: sarcolipin-like [Tursiops truncatus]" 100.00 31 100.00 100.00 2.86e-11 REF XP_005903864 "PREDICTED: sarcolipin [Bos mutus]" 100.00 31 100.00 100.00 2.86e-11 SP P42532 "RecName: Full=Sarcolipin" 100.00 31 100.00 100.00 2.86e-11 TPG DAA22460 "TPA: hypothetical protein BOS_14299 [Bos taurus]" 100.00 31 100.00 100.00 2.86e-11 stop_ save_ save_SERCA _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common SERCA _Molecular_mass . _Mol_thiol_state 'not reported' _Details . _Residue_count 1001 _Mol_residue_sequence ; MEAAHSKSTEECLAYFGVSE TTGLTPDQVKRHLEKYGHNE LPAEEGKSLWELVIEQFEDL LVRILLLAACISFVLAWFEE GEETITAFVEPFVILLILIA NAIVGVWQERNAENAIEALK EYEPEMGKVYRADRKSVQRI KARDIVPGDIVEVAVGDKVP ADIRILSIKSTTLRVDQSIL TGESVSVIKHTEPVPDPRAV NQDKKNMLFSGTNIAAGKAL GIVATTGVSTEIGKIRDQMA ATEQDKTPLQQKLDEFGEQL SKVISLICVAVWLINIGHFN DPVHGGSWIRGAIYYFKIAV ALAVAAIPEGLPAVITTCLA LGTRRMAKKNAIVRSLPSVE TLGCTSVICSDKTGTLTTNQ MSVCKMFIIDKVDGDFCSLN EFSITGSTYAPEGEVLKNDK PIRSGQFDGLVELATICALC NDSSLDFNETKGVYEKVGEA TETALTTLVEKMNVFNTEVR NLSKVERANACNSVIRQLMK KEFTLEFSRDRKSMSVYCSP AKSSRAAVGNKMFVKGAPEG VIDRCNYVRVGTTRVPMTGP VKEKILSVIKEWGTGRDTLR CLALATRDTPPKREEMVLDD SSRFMEYETDLTFVGVVGML DPPRKEVMGSIQLCRDAGIR VIMITGDNKGTAIAICRRIG IFGENEEVADRAYTGREFDD LPLAEQREACRRACCFARVE PSHKSKIVEYLQSYDEITAM TGDGVNDAPALKKAEIGIAM GSGTAVAKTASEMVLADDNF STIVAAVEEGRAIYNNMKQF IRYLISSNVGEVVCIFLTAA LGLPEALIPVQLLWVNLVTD GLPATALGFNPPDLDIMDRP PRSPKEPLISGWLFFRYMAI GGYVGAATVGAAAWWFMYAE DGPGVTYHQLTHFMQCTEDH PHFEGLDCEIFEAPEPMTMA LSVLVTIEMCNALNSLSENQ SLMRMPPWVNIWLLGSICLS MSLHFLILYVDPLPMIFKLK ALDLTQWLMVLKISLPVIGL DEILKFIARNYLEDPEDERR K ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLU 3 ALA 4 ALA 5 HIS 6 SER 7 LYS 8 SER 9 THR 10 GLU 11 GLU 12 CYS 13 LEU 14 ALA 15 TYR 16 PHE 17 GLY 18 VAL 19 SER 20 GLU 21 THR 22 THR 23 GLY 24 LEU 25 THR 26 PRO 27 ASP 28 GLN 29 VAL 30 LYS 31 ARG 32 HIS 33 LEU 34 GLU 35 LYS 36 TYR 37 GLY 38 HIS 39 ASN 40 GLU 41 LEU 42 PRO 43 ALA 44 GLU 45 GLU 46 GLY 47 LYS 48 SER 49 LEU 50 TRP 51 GLU 52 LEU 53 VAL 54 ILE 55 GLU 56 GLN 57 PHE 58 GLU 59 ASP 60 LEU 61 LEU 62 VAL 63 ARG 64 ILE 65 LEU 66 LEU 67 LEU 68 ALA 69 ALA 70 CYS 71 ILE 72 SER 73 PHE 74 VAL 75 LEU 76 ALA 77 TRP 78 PHE 79 GLU 80 GLU 81 GLY 82 GLU 83 GLU 84 THR 85 ILE 86 THR 87 ALA 88 PHE 89 VAL 90 GLU 91 PRO 92 PHE 93 VAL 94 ILE 95 LEU 96 LEU 97 ILE 98 LEU 99 ILE 100 ALA 101 ASN 102 ALA 103 ILE 104 VAL 105 GLY 106 VAL 107 TRP 108 GLN 109 GLU 110 ARG 111 ASN 112 ALA 113 GLU 114 ASN 115 ALA 116 ILE 117 GLU 118 ALA 119 LEU 120 LYS 121 GLU 122 TYR 123 GLU 124 PRO 125 GLU 126 MET 127 GLY 128 LYS 129 VAL 130 TYR 131 ARG 132 ALA 133 ASP 134 ARG 135 LYS 136 SER 137 VAL 138 GLN 139 ARG 140 ILE 141 LYS 142 ALA 143 ARG 144 ASP 145 ILE 146 VAL 147 PRO 148 GLY 149 ASP 150 ILE 151 VAL 152 GLU 153 VAL 154 ALA 155 VAL 156 GLY 157 ASP 158 LYS 159 VAL 160 PRO 161 ALA 162 ASP 163 ILE 164 ARG 165 ILE 166 LEU 167 SER 168 ILE 169 LYS 170 SER 171 THR 172 THR 173 LEU 174 ARG 175 VAL 176 ASP 177 GLN 178 SER 179 ILE 180 LEU 181 THR 182 GLY 183 GLU 184 SER 185 VAL 186 SER 187 VAL 188 ILE 189 LYS 190 HIS 191 THR 192 GLU 193 PRO 194 VAL 195 PRO 196 ASP 197 PRO 198 ARG 199 ALA 200 VAL 201 ASN 202 GLN 203 ASP 204 LYS 205 LYS 206 ASN 207 MET 208 LEU 209 PHE 210 SER 211 GLY 212 THR 213 ASN 214 ILE 215 ALA 216 ALA 217 GLY 218 LYS 219 ALA 220 LEU 221 GLY 222 ILE 223 VAL 224 ALA 225 THR 226 THR 227 GLY 228 VAL 229 SER 230 THR 231 GLU 232 ILE 233 GLY 234 LYS 235 ILE 236 ARG 237 ASP 238 GLN 239 MET 240 ALA 241 ALA 242 THR 243 GLU 244 GLN 245 ASP 246 LYS 247 THR 248 PRO 249 LEU 250 GLN 251 GLN 252 LYS 253 LEU 254 ASP 255 GLU 256 PHE 257 GLY 258 GLU 259 GLN 260 LEU 261 SER 262 LYS 263 VAL 264 ILE 265 SER 266 LEU 267 ILE 268 CYS 269 VAL 270 ALA 271 VAL 272 TRP 273 LEU 274 ILE 275 ASN 276 ILE 277 GLY 278 HIS 279 PHE 280 ASN 281 ASP 282 PRO 283 VAL 284 HIS 285 GLY 286 GLY 287 SER 288 TRP 289 ILE 290 ARG 291 GLY 292 ALA 293 ILE 294 TYR 295 TYR 296 PHE 297 LYS 298 ILE 299 ALA 300 VAL 301 ALA 302 LEU 303 ALA 304 VAL 305 ALA 306 ALA 307 ILE 308 PRO 309 GLU 310 GLY 311 LEU 312 PRO 313 ALA 314 VAL 315 ILE 316 THR 317 THR 318 CYS 319 LEU 320 ALA 321 LEU 322 GLY 323 THR 324 ARG 325 ARG 326 MET 327 ALA 328 LYS 329 LYS 330 ASN 331 ALA 332 ILE 333 VAL 334 ARG 335 SER 336 LEU 337 PRO 338 SER 339 VAL 340 GLU 341 THR 342 LEU 343 GLY 344 CYS 345 THR 346 SER 347 VAL 348 ILE 349 CYS 350 SER 351 ASP 352 LYS 353 THR 354 GLY 355 THR 356 LEU 357 THR 358 THR 359 ASN 360 GLN 361 MET 362 SER 363 VAL 364 CYS 365 LYS 366 MET 367 PHE 368 ILE 369 ILE 370 ASP 371 LYS 372 VAL 373 ASP 374 GLY 375 ASP 376 PHE 377 CYS 378 SER 379 LEU 380 ASN 381 GLU 382 PHE 383 SER 384 ILE 385 THR 386 GLY 387 SER 388 THR 389 TYR 390 ALA 391 PRO 392 GLU 393 GLY 394 GLU 395 VAL 396 LEU 397 LYS 398 ASN 399 ASP 400 LYS 401 PRO 402 ILE 403 ARG 404 SER 405 GLY 406 GLN 407 PHE 408 ASP 409 GLY 410 LEU 411 VAL 412 GLU 413 LEU 414 ALA 415 THR 416 ILE 417 CYS 418 ALA 419 LEU 420 CYS 421 ASN 422 ASP 423 SER 424 SER 425 LEU 426 ASP 427 PHE 428 ASN 429 GLU 430 THR 431 LYS 432 GLY 433 VAL 434 TYR 435 GLU 436 LYS 437 VAL 438 GLY 439 GLU 440 ALA 441 THR 442 GLU 443 THR 444 ALA 445 LEU 446 THR 447 THR 448 LEU 449 VAL 450 GLU 451 LYS 452 MET 453 ASN 454 VAL 455 PHE 456 ASN 457 THR 458 GLU 459 VAL 460 ARG 461 ASN 462 LEU 463 SER 464 LYS 465 VAL 466 GLU 467 ARG 468 ALA 469 ASN 470 ALA 471 CYS 472 ASN 473 SER 474 VAL 475 ILE 476 ARG 477 GLN 478 LEU 479 MET 480 LYS 481 LYS 482 GLU 483 PHE 484 THR 485 LEU 486 GLU 487 PHE 488 SER 489 ARG 490 ASP 491 ARG 492 LYS 493 SER 494 MET 495 SER 496 VAL 497 TYR 498 CYS 499 SER 500 PRO 501 ALA 502 LYS 503 SER 504 SER 505 ARG 506 ALA 507 ALA 508 VAL 509 GLY 510 ASN 511 LYS 512 MET 513 PHE 514 VAL 515 LYS 516 GLY 517 ALA 518 PRO 519 GLU 520 GLY 521 VAL 522 ILE 523 ASP 524 ARG 525 CYS 526 ASN 527 TYR 528 VAL 529 ARG 530 VAL 531 GLY 532 THR 533 THR 534 ARG 535 VAL 536 PRO 537 MET 538 THR 539 GLY 540 PRO 541 VAL 542 LYS 543 GLU 544 LYS 545 ILE 546 LEU 547 SER 548 VAL 549 ILE 550 LYS 551 GLU 552 TRP 553 GLY 554 THR 555 GLY 556 ARG 557 ASP 558 THR 559 LEU 560 ARG 561 CYS 562 LEU 563 ALA 564 LEU 565 ALA 566 THR 567 ARG 568 ASP 569 THR 570 PRO 571 PRO 572 LYS 573 ARG 574 GLU 575 GLU 576 MET 577 VAL 578 LEU 579 ASP 580 ASP 581 SER 582 SER 583 ARG 584 PHE 585 MET 586 GLU 587 TYR 588 GLU 589 THR 590 ASP 591 LEU 592 THR 593 PHE 594 VAL 595 GLY 596 VAL 597 VAL 598 GLY 599 MET 600 LEU 601 ASP 602 PRO 603 PRO 604 ARG 605 LYS 606 GLU 607 VAL 608 MET 609 GLY 610 SER 611 ILE 612 GLN 613 LEU 614 CYS 615 ARG 616 ASP 617 ALA 618 GLY 619 ILE 620 ARG 621 VAL 622 ILE 623 MET 624 ILE 625 THR 626 GLY 627 ASP 628 ASN 629 LYS 630 GLY 631 THR 632 ALA 633 ILE 634 ALA 635 ILE 636 CYS 637 ARG 638 ARG 639 ILE 640 GLY 641 ILE 642 PHE 643 GLY 644 GLU 645 ASN 646 GLU 647 GLU 648 VAL 649 ALA 650 ASP 651 ARG 652 ALA 653 TYR 654 THR 655 GLY 656 ARG 657 GLU 658 PHE 659 ASP 660 ASP 661 LEU 662 PRO 663 LEU 664 ALA 665 GLU 666 GLN 667 ARG 668 GLU 669 ALA 670 CYS 671 ARG 672 ARG 673 ALA 674 CYS 675 CYS 676 PHE 677 ALA 678 ARG 679 VAL 680 GLU 681 PRO 682 SER 683 HIS 684 LYS 685 SER 686 LYS 687 ILE 688 VAL 689 GLU 690 TYR 691 LEU 692 GLN 693 SER 694 TYR 695 ASP 696 GLU 697 ILE 698 THR 699 ALA 700 MET 701 THR 702 GLY 703 ASP 704 GLY 705 VAL 706 ASN 707 ASP 708 ALA 709 PRO 710 ALA 711 LEU 712 LYS 713 LYS 714 ALA 715 GLU 716 ILE 717 GLY 718 ILE 719 ALA 720 MET 721 GLY 722 SER 723 GLY 724 THR 725 ALA 726 VAL 727 ALA 728 LYS 729 THR 730 ALA 731 SER 732 GLU 733 MET 734 VAL 735 LEU 736 ALA 737 ASP 738 ASP 739 ASN 740 PHE 741 SER 742 THR 743 ILE 744 VAL 745 ALA 746 ALA 747 VAL 748 GLU 749 GLU 750 GLY 751 ARG 752 ALA 753 ILE 754 TYR 755 ASN 756 ASN 757 MET 758 LYS 759 GLN 760 PHE 761 ILE 762 ARG 763 TYR 764 LEU 765 ILE 766 SER 767 SER 768 ASN 769 VAL 770 GLY 771 GLU 772 VAL 773 VAL 774 CYS 775 ILE 776 PHE 777 LEU 778 THR 779 ALA 780 ALA 781 LEU 782 GLY 783 LEU 784 PRO 785 GLU 786 ALA 787 LEU 788 ILE 789 PRO 790 VAL 791 GLN 792 LEU 793 LEU 794 TRP 795 VAL 796 ASN 797 LEU 798 VAL 799 THR 800 ASP 801 GLY 802 LEU 803 PRO 804 ALA 805 THR 806 ALA 807 LEU 808 GLY 809 PHE 810 ASN 811 PRO 812 PRO 813 ASP 814 LEU 815 ASP 816 ILE 817 MET 818 ASP 819 ARG 820 PRO 821 PRO 822 ARG 823 SER 824 PRO 825 LYS 826 GLU 827 PRO 828 LEU 829 ILE 830 SER 831 GLY 832 TRP 833 LEU 834 PHE 835 PHE 836 ARG 837 TYR 838 MET 839 ALA 840 ILE 841 GLY 842 GLY 843 TYR 844 VAL 845 GLY 846 ALA 847 ALA 848 THR 849 VAL 850 GLY 851 ALA 852 ALA 853 ALA 854 TRP 855 TRP 856 PHE 857 MET 858 TYR 859 ALA 860 GLU 861 ASP 862 GLY 863 PRO 864 GLY 865 VAL 866 THR 867 TYR 868 HIS 869 GLN 870 LEU 871 THR 872 HIS 873 PHE 874 MET 875 GLN 876 CYS 877 THR 878 GLU 879 ASP 880 HIS 881 PRO 882 HIS 883 PHE 884 GLU 885 GLY 886 LEU 887 ASP 888 CYS 889 GLU 890 ILE 891 PHE 892 GLU 893 ALA 894 PRO 895 GLU 896 PRO 897 MET 898 THR 899 MET 900 ALA 901 LEU 902 SER 903 VAL 904 LEU 905 VAL 906 THR 907 ILE 908 GLU 909 MET 910 CYS 911 ASN 912 ALA 913 LEU 914 ASN 915 SER 916 LEU 917 SER 918 GLU 919 ASN 920 GLN 921 SER 922 LEU 923 MET 924 ARG 925 MET 926 PRO 927 PRO 928 TRP 929 VAL 930 ASN 931 ILE 932 TRP 933 LEU 934 LEU 935 GLY 936 SER 937 ILE 938 CYS 939 LEU 940 SER 941 MET 942 SER 943 LEU 944 HIS 945 PHE 946 LEU 947 ILE 948 LEU 949 TYR 950 VAL 951 ASP 952 PRO 953 LEU 954 PRO 955 MET 956 ILE 957 PHE 958 LYS 959 LEU 960 LYS 961 ALA 962 LEU 963 ASP 964 LEU 965 THR 966 GLN 967 TRP 968 LEU 969 MET 970 VAL 971 LEU 972 LYS 973 ILE 974 SER 975 LEU 976 PRO 977 VAL 978 ILE 979 GLY 980 LEU 981 ASP 982 GLU 983 ILE 984 LEU 985 LYS 986 PHE 987 ILE 988 ALA 989 ARG 990 ASN 991 TYR 992 LEU 993 GLU 994 ASP 995 PRO 996 GLU 997 ASP 998 GLU 999 ARG 1000 ARG 1001 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-04-16 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1IWO "Crystal Structure Of The Sr Ca2+-Atpase In The Absence Of Ca2+" 99.20 994 100.00 100.00 0.00e+00 PDB 1KJU "Ca2+-Atpase In The E2 State" 99.20 994 100.00 100.00 0.00e+00 PDB 1SU4 "Crystal Structure Of Calcium Atpase With Two Bound Calcium Ions" 99.20 994 100.00 100.00 0.00e+00 PDB 1T5S "Structure Of The (sr)ca2+-atpase Ca2-e1-amppcp Form" 99.20 994 100.00 100.00 0.00e+00 PDB 1T5T "Structure Of The (Sr)ca2+-Atpase Ca2-E1-Adp:alf4- Form" 99.20 994 100.00 100.00 0.00e+00 PDB 1VFP "Crystal Structure Of The Sr Ca2+-Atpase With Bound Amppcp" 99.20 994 100.00 100.00 0.00e+00 PDB 1WPG "Crystal Structure Of The Sr Ca2+-Atpase With Mgf4" 99.20 994 100.00 100.00 0.00e+00 PDB 1XP5 "Structure Of The (Sr)ca2+-Atpase E2-Alf4- Form" 99.20 994 100.00 100.00 0.00e+00 PDB 2AGV "Crystal Structure Of The Sr Ca2+-atpase With Bhq And Tg" 99.20 994 100.00 100.00 0.00e+00 PDB 2BY4 "Sr Ca(2+)-Atpase In The Hne2 State Complexed With The Thapsigargin Derivative Boc-12adt." 99.20 994 100.00 100.00 0.00e+00 PDB 2C88 "Crystal Structure Of (Sr) Calcium-Atpase E2(Tg):amppcp Form" 99.20 994 100.00 100.00 0.00e+00 PDB 2C8K "Crystal Structure Of (Sr) Calcium-Atpase E2(Tg) With Partially Occupied Amppcp Site" 99.20 994 100.00 100.00 0.00e+00 PDB 2C8L "Crystal Structure Of (Sr) Calcium-Atpase E2(Tg) Form" 99.20 994 100.00 100.00 0.00e+00 PDB 2C9M "Structure Of (Sr) Calcium-Atpase In The Ca2e1 State Solved In A P1 Crystal Form." 99.20 994 100.00 100.00 0.00e+00 PDB 2DQS "Crystal Structure Of The Calcium Pump With Amppcp In The Absence Of Calcium" 99.20 995 100.00 100.00 0.00e+00 PDB 2EAR "P21 Crystal Of The Sr Ca2+-Atpase With Bound Tg" 99.20 995 100.00 100.00 0.00e+00 PDB 2EAS "Crystal Structure Of The Sr Ca2+-Atpase With Bound Cpa" 99.20 994 100.00 100.00 0.00e+00 PDB 2EAT "Crystal Structure Of The Sr Ca2+-Atpase With Bound Cpa And Tg" 99.20 995 100.00 100.00 0.00e+00 PDB 2EAU "Crystal Structure Of The Sr Ca2+-Atpase With Bound Cpa In The Presence Of Curcumin" 99.20 994 100.00 100.00 0.00e+00 PDB 2O9J "Crystal Structure Of Calcium Atpase With Bound Magnesium Fluoride And Cyclopiazonic Acid" 99.20 994 100.00 100.00 0.00e+00 PDB 2OA0 "Crystal Structure Of Calcium Atpase With Bound Adp And Cyclopiazonic Acid" 99.20 994 100.00 100.00 0.00e+00 PDB 2YFY "Serca In The Hne2 State Complexed With Debutanoyl Thapsigargin" 99.20 994 100.00 100.00 0.00e+00 PDB 2ZBD "Crystal Structure Of The Sr Calcium Pump With Bound Aluminium Fluoride, Adp And Calcium" 99.20 995 100.00 100.00 0.00e+00 PDB 2ZBE "Calcium Pump Crystal Structure With Bound Bef3 In The Absence Of Calcium And Tg" 99.20 995 100.00 100.00 0.00e+00 PDB 2ZBF "Calcium Pump Crystal Structure With Bound Bef3 And Tg In The Absence Of Calcium" 99.20 995 100.00 100.00 0.00e+00 PDB 2ZBG "Calcium Pump Crystal Structure With Bound Alf4 And Tg In The Absence Of Calcium" 99.20 995 100.00 100.00 0.00e+00 PDB 3AR2 "Calcium Pump Crystal Structure With Bound Amppcp And Ca2+" 99.20 995 100.00 100.00 0.00e+00 PDB 3AR3 "Calcium Pump Crystal Structure With Bound Adp And Tg" 99.20 995 100.00 100.00 0.00e+00 PDB 3AR4 "Calcium Pump Crystal Structure With Bound Atp And Tg In The Absence Of Ca2+" 99.20 995 100.00 100.00 0.00e+00 PDB 3AR5 "Calcium Pump Crystal Structure With Bound Tnp-amp And Tg" 99.20 995 100.00 100.00 0.00e+00 PDB 3AR6 "Calcium Pump Crystal Structure With Bound Tnp-adp And Tg In The Absence Of Calcium" 99.20 995 100.00 100.00 0.00e+00 PDB 3AR7 "Calcium Pump Crystal Structure With Bound Tnp-atp And Tg In The Absence Of Ca2+" 99.20 995 100.00 100.00 0.00e+00 PDB 3AR8 "Calcium Pump Crystal Structure With Bound Alf4, Tnp-amp And Tg" 99.20 995 100.00 100.00 0.00e+00 PDB 3AR9 "Calcium Pump Crystal Structure With Bound Bef3, Tnp-amp And Tg In The Absence Of Calcium" 99.20 995 100.00 100.00 0.00e+00 PDB 3B9B "Structure Of The E2 Beryllium Fluoride Complex Of The Serca Ca2+-Atpase" 99.20 994 100.00 100.00 0.00e+00 PDB 3B9R "Serca Ca2+-Atpase E2 Aluminium Fluoride Complex Without Thapsigargin" 99.20 994 100.00 100.00 0.00e+00 PDB 3BA6 "Structure Of The Ca2e1p Phosphoenzyme Intermediate Of The Serca Ca2+-Atpase" 99.20 994 99.90 99.90 0.00e+00 PDB 3FGO "Crystal Structure Of The E2 Magnesium Fluoride Complex Of The (Sr) Ca2+-Atpase With Bound Cpa And Amppcp" 99.20 994 100.00 100.00 0.00e+00 PDB 3FPB "The Structure Of Sarcoplasmic Reticulum Ca2+-atpase Bound To Cyclopiazonic Acid With Atp" 99.20 994 100.00 100.00 0.00e+00 PDB 3FPS "The Structure Of Sarcoplasmic Reticulum Ca2+-atpase Bound To Cyclopiazonic And Adp" 99.20 994 100.00 100.00 0.00e+00 PDB 3N5K "Structure Of The (sr)ca2+-atpase E2-alf4- Form" 99.20 994 100.00 100.00 0.00e+00 PDB 3N8G "Structure Of The (sr)ca2+-atpase Ca2-e1-caamppcp Form" 99.20 994 100.00 100.00 0.00e+00 PDB 3NAL "Sr Ca(2+)-Atpase In The Hne2 State Complexed With The Thapsigargin Derivative Dtb" 99.20 994 100.00 100.00 0.00e+00 PDB 3NAM "Sr Ca(2+)-Atpase In The Hne2 State Complexed With The Thapsigargin Derivative Dotg" 99.20 994 100.00 100.00 0.00e+00 PDB 3NAN "Sr Ca(2+)-Atpase In The Hne2 State Complexed With A Thapsigargin Derivative Boc-(Phi)tg" 99.20 994 100.00 100.00 0.00e+00 PDB 3W5A "Crystal Structure Of The Calcium Pump And Sarcolipin From Rabbit Fast Twitch Skeletal Muscle In The E1.mg2+ State" 99.20 995 100.00 100.00 0.00e+00 PDB 3W5B "Crystal Structure Of The Recombinant Serca1a (calcium Pump Of Fast Twitch Skeletal Muscle) In The E1.mg2+ State" 99.20 1000 100.00 100.00 0.00e+00 PDB 3W5C "Crystal Structure Of The Calcium Pump In The E2 State Free From Exogenous Inhibitors" 99.20 995 100.00 100.00 0.00e+00 PDB 3W5D "Crystal Structure Of The Calcium Pump In The E2+pi State" 99.20 995 100.00 100.00 0.00e+00 PDB 4BEW "Serca Bound To Phosphate Analogue" 99.30 994 100.00 100.00 0.00e+00 PDB 4H1W "E1 Structure Of The (sr) Ca2+-atpase In Complex With Sarcolipin" 99.20 994 100.00 100.00 0.00e+00 PDB 4J2T "Inhibitor-bound Ca2+ Atpase" 99.20 994 100.00 100.00 0.00e+00 PDB 4KYT "The Structure Of Superinhibitory Phospholamban Bound To The Calcium Pump Serca1a" 99.20 994 100.00 100.00 0.00e+00 PDB 4NAB "Structure Of The (sr)ca2+-atpase Mutant E309q In The Ca2-e1-mgamppcp Form" 99.20 1000 99.90 100.00 0.00e+00 GB AAA31165 "Ca2+ ATPase [Oryctolagus cuniculus]" 100.00 1001 100.00 100.00 0.00e+00 GB AAH37354 "Similar to ATPase, Ca++ transporting, cardiac muscle, fast twitch 1, partial [Homo sapiens]" 79.92 844 97.25 99.00 0.00e+00 GB ABW96358 "SERCA1a [Oryctolagus cuniculus]" 99.20 994 100.00 100.00 0.00e+00 GB EDM17472 "ATPase, Ca++ transporting, cardiac muscle, fast twitch 1, isoform CRA_b [Rattus norvegicus]" 100.00 1001 96.90 99.00 0.00e+00 GB ELW66399 "Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 [Tupaia chinensis]" 100.00 1001 97.10 99.00 0.00e+00 REF NP_001082787 "sarcoplasmic/endoplasmic reticulum calcium ATPase 1 [Oryctolagus cuniculus]" 100.00 1001 100.00 100.00 0.00e+00 REF XP_004586901 "PREDICTED: sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform X1 [Ochotona princeps]" 100.00 1001 98.40 99.60 0.00e+00 REF XP_004586902 "PREDICTED: sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform X2 [Ochotona princeps]" 99.20 994 98.39 99.60 0.00e+00 REF XP_005621437 "PREDICTED: sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform X14 [Canis lupus familiaris]" 99.20 1052 96.98 99.09 0.00e+00 REF XP_006148309 "PREDICTED: sarcoplasmic/endoplasmic reticulum calcium ATPase 1 [Tupaia chinensis]" 87.51 876 96.92 98.97 0.00e+00 SP P04191 "RecName: Full=Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; Short=SERCA1; Short=SR Ca(2+)-ATPase 1; AltName: Full=Calciu" 100.00 1001 100.00 100.00 0.00e+00 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $SLN Rabbit 9986 Eukaryota Metazoa Oryctolagus cuniculus $SERCA Rabbit 9986 Eukaryota Metazoa Oryctolagus cuniculus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Variant _Vector_name $SLN 'recombinant technology' . Escherichia coli BL21 DE3 na stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '25 ul aliquotes of SERCA were titrated into solution' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $SLN 0.3 mM . . [U-15N] 'phosphate-buffered saline' 20 mM . . 'natural abundance' DPC 300 mM . . 'natural abundance' $SERCA . mg/ml 0 56 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.0 . pH pressure ambient . atm temperature 310 . K stop_ save_