data_17141 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for HYL1-dsRBD2 ; _BMRB_accession_number 17141 _BMRB_flat_file_name bmr17141.str _Entry_type original _Submission_date 2010-08-22 _Accession_date 2010-08-22 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Rasia Rodolfo M. . 2 Mateos Julieta L. . 3 Bologna Nicolas G. . 4 Burdisso Paula . . 5 Imbert Lionel . . 6 Palatnik Javier F. . 7 Boisbouvier Jerome . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 residual_dipolar_couplings 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 63 "13C chemical shifts" 195 "15N chemical shifts" 63 "residual dipolar couplings" 181 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-09-28 original author . stop_ _Original_release_date 2010-09-28 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structure and RNA Interactions of the Plant MicroRNA Processing-Associated Protein HYL1.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 20735118 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Rasia Rodolfo M. . 2 Mateos Julieta . . 3 Bologna Nicolas G. . 4 Burdisso Paula . . 5 Imbert Lionel . . 6 Palatnik Javier F. . 7 Boisbouvier Jerome . . stop_ _Journal_abbreviation Biochemistry _Journal_name_full Biochemistry _Journal_volume 49 _Journal_issue 38 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 8237 _Page_last 8239 _Year 2010 _Details . loop_ _Keyword dsRBD miRNA stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'HYL1 second dsRBD' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'HYL1 second dsRBD' $hyl1-2 stop_ _System_molecular_weight 8463.7 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_hyl1-2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common hyl1-2 _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'double-stranded RNA binding, protein binding, miRNA binding;' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 77 _Mol_residue_sequence ; GHMHETGLCKNLLQEYAQKM NYAIPLYQCQKVETLGRVTQ FTCTVEIGGIKYTGAATRTK KDAEISAGRTALLAIQS ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 HIS 3 MET 4 HIS 5 GLU 6 THR 7 GLY 8 LEU 9 CYS 10 LYS 11 ASN 12 LEU 13 LEU 14 GLN 15 GLU 16 TYR 17 ALA 18 GLN 19 LYS 20 MET 21 ASN 22 TYR 23 ALA 24 ILE 25 PRO 26 LEU 27 TYR 28 GLN 29 CYS 30 GLN 31 LYS 32 VAL 33 GLU 34 THR 35 LEU 36 GLY 37 ARG 38 VAL 39 THR 40 GLN 41 PHE 42 THR 43 CYS 44 THR 45 VAL 46 GLU 47 ILE 48 GLY 49 GLY 50 ILE 51 LYS 52 TYR 53 THR 54 GLY 55 ALA 56 ALA 57 THR 58 ARG 59 THR 60 LYS 61 LYS 62 ASP 63 ALA 64 GLU 65 ILE 66 SER 67 ALA 68 GLY 69 ARG 70 THR 71 ALA 72 LEU 73 LEU 74 ALA 75 ILE 76 GLN 77 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2L2M "Solution Structure Of The Second Dsrbd Of Hyl1" 100.00 77 100.00 100.00 2.83e-48 PDB 3ADJ "Structure Of Arabidopsis Hyl1 And Its Molecular Implications For Mirna Processing" 92.21 76 100.00 100.00 9.63e-43 DBJ BAH57238 "AT1G09700 [Arabidopsis thaliana]" 97.40 403 98.67 100.00 1.54e-43 GB AAB60726 "F21M12.9 gene product [Arabidopsis thaliana]" 97.40 419 98.67 100.00 1.33e-43 GB AAG49890 "hyponastic leave 1 [Arabidopsis thaliana]" 97.40 419 98.67 100.00 1.33e-43 GB AAK96822 "Unknown protein [Arabidopsis thaliana]" 97.40 419 98.67 100.00 1.33e-43 GB AAM10087 "unknown protein [Arabidopsis thaliana]" 97.40 419 98.67 100.00 1.33e-43 GB AEE28481 "dsRNA-binding hyponastic leave 1 protein [Arabidopsis thaliana]" 97.40 419 98.67 100.00 1.33e-43 REF NP_563850 "double-stranded RNA-binding protein 1 [Arabidopsis thaliana]" 97.40 419 98.67 100.00 1.33e-43 SP O04492 "RecName: Full=Double-stranded RNA-binding protein 1; AltName: Full=Protein HYPONASTIC LEAVES 1; AltName: Full=dsRNA-binding pro" 97.40 419 98.67 100.00 1.33e-43 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $hyl1-2 'thale cress' 3702 Eukaryota Viridiplantae Arabidopsis thaliana stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $hyl1-2 'recombinant technology' . Escherichia coli . pET-TEV stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $hyl1-2 0.8 mM '[U-100% 13C; U-100% 15N]' DTT 10 mM 'natural abundance' 'sodium phosphate' 100 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' 'sodium azide' 0.05 % 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_aligned _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $hyl1-2 0.8 mM '[U-100% 13C; U-100% 15N]' DTT 10 mM 'natural abundance' 'sodium phosphate' 100 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' 'sodium azide' 0.05 % 'natural abundance' C12E5 5 % 'natural abundance' Hexanol 0.2 % 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task 'data analysis' processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model DirectDrive _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HN(COCA)CB_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(COCA)CB' _Sample_label $sample_1 save_ save_3D_HN(CA)CO_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)CO' _Sample_label $sample_1 save_ save_3D_HNCO_JCACO_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO JCACO' _Sample_label $sample_1 save_ save_3D_HNCO_JNH_9 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO JNH' _Sample_label $sample_1 save_ save_3D_HNCOCA_JCAHA_10 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCOCA JCAHA' _Sample_label $sample_1 save_ save_3D_HNCO_JCACO_11 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO JCACO' _Sample_label $sample_aligned save_ save_3D_HNCO_JNH_12 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO JNH' _Sample_label $sample_aligned save_ save_3D_HNCOCA_JCAHA_13 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCOCA JCAHA' _Sample_label $sample_aligned save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 4.77 internal indirect . . . 0.251449530 water H 1 protons ppm 4.77 internal direct . . . 1.0 water N 15 protons ppm 4.77 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $NMRPipe $NMRView stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCO' '3D HNCA' '3D HNCACB' '3D HN(CO)CA' '3D HN(COCA)CB' '3D HN(CA)CO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'HYL1 second dsRBD' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 7 7 GLY CA C 46.000 0.300 1 2 7 7 GLY C C 174.700 0.300 1 3 8 8 LEU N N 121.850 0.300 1 4 8 8 LEU H H 7.990 0.030 1 5 8 8 LEU CA C 59.000 0.300 1 6 8 8 LEU CB C 42.100 0.300 1 7 11 11 ASN CA C 56.100 0.300 1 8 11 11 ASN CB C 38.300 0.300 1 9 11 11 ASN C C 177.500 0.300 1 10 12 12 LEU N N 121.130 0.300 1 11 12 12 LEU H H 8.280 0.030 1 12 12 12 LEU CA C 58.000 0.300 1 13 12 12 LEU CB C 42.300 0.300 1 14 12 12 LEU C C 180.650 0.300 1 15 13 13 LEU N N 120.250 0.300 1 16 13 13 LEU H H 8.240 0.030 1 17 13 13 LEU CA C 57.800 0.300 1 18 13 13 LEU CB C 41.700 0.300 1 19 13 13 LEU C C 178.450 0.300 1 20 14 14 GLN N N 119.290 0.300 1 21 14 14 GLN H H 7.950 0.030 1 22 14 14 GLN CA C 59.800 0.300 1 23 14 14 GLN CB C 28.350 0.300 1 24 14 14 GLN C C 178.600 0.300 1 25 15 15 GLU N N 120.600 0.300 1 26 15 15 GLU H H 8.320 0.030 1 27 15 15 GLU CA C 59.600 0.300 1 28 15 15 GLU CB C 29.800 0.300 1 29 15 15 GLU C C 178.850 0.300 1 30 16 16 TYR N N 121.440 0.300 1 31 16 16 TYR H H 8.050 0.030 1 32 16 16 TYR CA C 61.500 0.300 1 33 16 16 TYR CB C 38.250 0.300 1 34 16 16 TYR C C 176.400 0.300 1 35 17 17 ALA N N 120.120 0.300 1 36 17 17 ALA H H 8.390 0.030 1 37 17 17 ALA CA C 55.650 0.300 1 38 17 17 ALA CB C 18.100 0.300 1 39 17 17 ALA C C 179.000 0.300 1 40 18 18 GLN N N 115.810 0.300 1 41 18 18 GLN H H 7.970 0.030 1 42 18 18 GLN CB C 28.700 0.300 1 43 18 18 GLN C C 179.850 0.300 1 44 19 19 LYS N N 121.560 0.300 1 45 19 19 LYS H H 7.990 0.030 1 46 19 19 LYS CA C 59.000 0.300 1 47 19 19 LYS CB C 32.400 0.300 1 48 19 19 LYS C C 178.000 0.300 1 49 20 20 MET N N 114.720 0.300 1 50 20 20 MET H H 7.230 0.030 1 51 20 20 MET CA C 53.000 0.300 1 52 20 20 MET CB C 29.650 0.300 1 53 20 20 MET C C 175.300 0.300 1 54 21 21 ASN N N 114.180 0.300 1 55 21 21 ASN H H 7.660 0.030 1 56 21 21 ASN CA C 54.600 0.300 1 57 21 21 ASN CB C 37.250 0.300 1 58 21 21 ASN C C 175.200 0.300 1 59 22 22 TYR N N 116.950 0.300 1 60 22 22 TYR H H 8.450 0.030 1 61 22 22 TYR CA C 52.900 0.300 1 62 22 22 TYR CB C 39.450 0.300 1 63 22 22 TYR C C 176.100 0.300 1 64 23 23 ALA N N 122.380 0.300 1 65 23 23 ALA H H 8.290 0.030 1 66 23 23 ALA CA C 51.800 0.300 1 67 23 23 ALA CB C 19.600 0.300 1 68 23 23 ALA C C 177.000 0.300 1 69 24 24 ILE N N 119.790 0.300 1 70 24 24 ILE H H 7.990 0.030 1 71 24 24 ILE CA C 60.100 0.300 1 72 24 24 ILE CB C 38.600 0.300 1 73 24 24 ILE C C 175.300 0.300 1 74 25 25 PRO CA C 63.800 0.300 1 75 25 25 PRO CB C 32.800 0.300 1 76 25 25 PRO C C 174.800 0.300 1 77 26 26 LEU N N 124.370 0.300 1 78 26 26 LEU H H 7.710 0.030 1 79 26 26 LEU CA C 53.800 0.300 1 80 26 26 LEU CB C 44.950 0.300 1 81 26 26 LEU C C 175.850 0.300 1 82 27 27 TYR N N 126.330 0.300 1 83 27 27 TYR H H 9.170 0.030 1 84 27 27 TYR CA C 57.650 0.300 1 85 27 27 TYR CB C 40.250 0.300 1 86 27 27 TYR C C 175.900 0.300 1 87 28 28 GLN N N 123.270 0.300 1 88 28 28 GLN H H 8.870 0.030 1 89 28 28 GLN CA C 55.500 0.300 1 90 28 28 GLN CB C 31.700 0.300 1 91 28 28 GLN C C 175.300 0.300 1 92 29 29 CYS CA C 56.100 0.300 1 93 29 29 CYS CB C 49.600 0.300 1 94 29 29 CYS C C 173.100 0.300 1 95 30 30 GLN N N 120.920 0.300 1 96 30 30 GLN H H 9.260 0.030 1 97 30 30 GLN CA C 54.500 0.300 1 98 30 30 GLN CB C 32.400 0.300 1 99 30 30 GLN C C 173.600 0.300 1 100 31 31 LYS N N 123.470 0.300 1 101 31 31 LYS H H 8.520 0.030 1 102 31 31 LYS CA C 55.200 0.300 1 103 31 31 LYS CB C 34.350 0.300 1 104 31 31 LYS C C 176.000 0.300 1 105 32 32 VAL N N 125.250 0.300 1 106 32 32 VAL H H 8.780 0.030 1 107 32 32 VAL CA C 61.300 0.300 1 108 32 32 VAL CB C 34.350 0.300 1 109 32 32 VAL C C 174.700 0.300 1 110 33 33 GLU N N 126.810 0.300 1 111 33 33 GLU H H 8.610 0.030 1 112 33 33 GLU CA C 55.150 0.300 1 113 33 33 GLU CB C 31.700 0.300 1 114 33 33 GLU C C 176.100 0.300 1 115 34 34 THR N N 118.420 0.300 1 116 34 34 THR H H 8.560 0.030 1 117 34 34 THR CA C 60.900 0.300 1 118 34 34 THR CB C 70.800 0.300 1 119 34 34 THR C C 174.300 0.300 1 120 36 36 GLY CA C 45.500 0.300 1 121 36 36 GLY C C 174.000 0.300 1 122 37 37 ARG N N 117.670 0.300 1 123 37 37 ARG H H 8.160 0.030 1 124 37 37 ARG CA C 55.600 0.300 1 125 37 37 ARG CB C 30.450 0.300 1 126 37 37 ARG C C 175.700 0.300 1 127 38 38 VAL N N 120.350 0.300 1 128 38 38 VAL H H 7.790 0.030 1 129 38 38 VAL CA C 62.200 0.300 1 130 38 38 VAL CB C 32.950 0.300 1 131 38 38 VAL C C 176.200 0.300 1 132 39 39 THR N N 123.680 0.300 1 133 39 39 THR H H 8.550 0.030 1 134 39 39 THR CA C 63.450 0.300 1 135 39 39 THR CB C 69.350 0.300 1 136 39 39 THR C C 173.700 0.300 1 137 40 40 GLN N N 122.670 0.300 1 138 40 40 GLN H H 8.250 0.030 1 139 40 40 GLN CA C 54.250 0.300 1 140 40 40 GLN CB C 33.950 0.300 1 141 40 40 GLN C C 173.800 0.300 1 142 41 41 PHE N N 119.660 0.300 1 143 41 41 PHE H H 9.330 0.030 1 144 41 41 PHE CA C 57.100 0.300 1 145 41 41 PHE CB C 44.000 0.300 1 146 41 41 PHE C C 174.850 0.300 1 147 42 42 THR N N 112.280 0.300 1 148 42 42 THR H H 8.500 0.030 1 149 42 42 THR CA C 61.500 0.300 1 150 42 42 THR CB C 71.450 0.300 1 151 42 42 THR C C 174.300 0.300 1 152 43 43 CYS N N 123.420 0.300 1 153 43 43 CYS H H 9.820 0.030 1 154 43 43 CYS CA C 55.950 0.300 1 155 43 43 CYS CB C 49.200 0.300 1 156 43 43 CYS C C 172.100 0.300 1 157 44 44 THR N N 114.230 0.300 1 158 44 44 THR H H 9.280 0.030 1 159 44 44 THR CA C 59.900 0.300 1 160 44 44 THR CB C 72.050 0.300 1 161 44 44 THR C C 173.900 0.300 1 162 45 45 VAL N N 122.140 0.300 1 163 45 45 VAL H H 9.160 0.030 1 164 45 45 VAL CA C 58.550 0.300 1 165 45 45 VAL CB C 35.600 0.300 1 166 45 45 VAL C C 172.200 0.300 1 167 46 46 GLU N N 129.420 0.300 1 168 46 46 GLU H H 8.530 0.030 1 169 46 46 GLU CA C 54.200 0.300 1 170 46 46 GLU CB C 32.100 0.300 1 171 46 46 GLU C C 176.000 0.300 1 172 47 47 ILE N N 125.200 0.300 1 173 47 47 ILE H H 8.970 0.030 1 174 47 47 ILE CA C 58.600 0.300 1 175 47 47 ILE CB C 40.650 0.300 1 176 47 47 ILE C C 176.900 0.300 1 177 48 48 GLY N N 119.540 0.300 1 178 48 48 GLY H H 9.540 0.030 1 179 48 48 GLY CA C 46.200 0.300 1 180 48 48 GLY C C 175.100 0.300 1 181 49 49 GLY N N 104.900 0.300 1 182 49 49 GLY H H 8.520 0.030 1 183 49 49 GLY CA C 44.850 0.300 1 184 49 49 GLY C C 173.200 0.300 1 185 50 50 ILE N N 122.860 0.300 1 186 50 50 ILE H H 7.430 0.030 1 187 50 50 ILE CA C 59.750 0.300 1 188 50 50 ILE CB C 38.750 0.300 1 189 50 50 ILE C C 173.600 0.300 1 190 51 51 LYS N N 124.640 0.300 1 191 51 51 LYS H H 8.010 0.030 1 192 51 51 LYS CA C 55.500 0.300 1 193 51 51 LYS CB C 34.550 0.300 1 194 51 51 LYS C C 175.700 0.300 1 195 52 52 TYR N N 124.200 0.300 1 196 52 52 TYR H H 9.110 0.030 1 197 52 52 TYR CA C 57.100 0.300 1 198 52 52 TYR CB C 40.250 0.300 1 199 52 52 TYR C C 174.900 0.300 1 200 53 53 THR N N 120.030 0.300 1 201 53 53 THR H H 8.810 0.030 1 202 53 53 THR CA C 62.300 0.300 1 203 53 53 THR CB C 70.300 0.300 1 204 53 53 THR C C 175.000 0.300 1 205 54 54 GLY N N 112.740 0.300 1 206 54 54 GLY H H 8.410 0.030 1 207 54 54 GLY CA C 44.000 0.300 1 208 54 54 GLY C C 172.200 0.300 1 209 55 55 ALA N N 119.010 0.300 1 210 55 55 ALA H H 8.350 0.030 1 211 55 55 ALA CA C 50.950 0.300 1 212 55 55 ALA CB C 20.650 0.300 1 213 55 55 ALA C C 177.300 0.300 1 214 56 56 ALA N N 122.550 0.300 1 215 56 56 ALA H H 8.130 0.030 1 216 56 56 ALA CA C 51.500 0.300 1 217 56 56 ALA CB C 19.000 0.300 1 218 56 56 ALA C C 179.000 0.300 1 219 57 57 THR N N 115.510 0.300 1 220 57 57 THR H H 9.270 0.030 1 221 57 57 THR CA C 60.550 0.300 1 222 57 57 THR CB C 35.450 0.300 1 223 57 57 THR C C 174.700 0.300 1 224 58 58 ARG N N 119.220 0.300 1 225 58 58 ARG H H 8.620 0.030 1 226 58 58 ARG CA C 58.600 0.300 1 227 58 58 ARG CB C 31.200 0.300 1 228 58 58 ARG C C 176.900 0.300 1 229 59 59 THR N N 104.160 0.300 1 230 59 59 THR H H 7.730 0.030 1 231 59 59 THR CA C 58.950 0.300 1 232 59 59 THR CB C 35.950 0.300 1 233 59 59 THR C C 174.500 0.300 1 234 60 60 LYS N N 126.240 0.300 1 235 60 60 LYS H H 8.240 0.030 1 236 60 60 LYS CA C 60.000 0.300 1 237 60 60 LYS CB C 31.750 0.300 1 238 60 60 LYS C C 177.800 0.300 1 239 61 61 LYS N N 118.170 0.300 1 240 61 61 LYS H H 8.250 0.030 1 241 61 61 LYS CA C 59.200 0.300 1 242 61 61 LYS CB C 32.150 0.300 1 243 61 61 LYS C C 179.250 0.300 1 244 62 62 ASP N N 116.780 0.300 1 245 62 62 ASP H H 7.500 0.030 1 246 62 62 ASP CA C 57.000 0.300 1 247 62 62 ASP CB C 39.950 0.300 1 248 62 62 ASP C C 178.600 0.300 1 249 63 63 ALA N N 129.210 0.300 1 250 63 63 ALA H H 7.860 0.030 1 251 63 63 ALA CA C 55.650 0.300 1 252 63 63 ALA CB C 19.900 0.300 1 253 63 63 ALA C C 181.700 0.300 1 254 64 64 GLU N N 124.190 0.300 1 255 64 64 GLU H H 8.560 0.030 1 256 64 64 GLU CA C 60.250 0.300 1 257 64 64 GLU CB C 30.500 0.300 1 258 64 64 GLU C C 179.300 0.300 1 259 65 65 ILE N N 120.500 0.300 1 260 65 65 ILE H H 7.760 0.030 1 261 65 65 ILE CA C 65.200 0.300 1 262 65 65 ILE C C 177.600 0.300 1 263 66 66 SER N N 112.650 0.300 1 264 66 66 SER H H 8.120 0.030 1 265 66 66 SER CA C 61.500 0.300 1 266 66 66 SER CB C 31.400 0.300 1 267 66 66 SER C C 178.600 0.300 1 268 67 67 ALA N N 123.210 0.300 1 269 67 67 ALA H H 8.000 0.030 1 270 67 67 ALA CA C 56.100 0.300 1 271 67 67 ALA CB C 17.900 0.300 1 272 67 67 ALA C C 178.400 0.300 1 273 68 68 GLY N N 104.750 0.300 1 274 68 68 GLY H H 8.470 0.030 1 275 68 68 GLY CA C 47.700 0.300 1 276 68 68 GLY C C 175.200 0.300 1 277 69 69 ARG N N 121.830 0.300 1 278 69 69 ARG H H 8.930 0.030 1 279 69 69 ARG CA C 59.700 0.300 1 280 69 69 ARG CB C 30.250 0.300 1 281 69 69 ARG C C 178.500 0.300 1 282 70 70 THR N N 116.930 0.300 1 283 70 70 THR H H 7.980 0.030 1 284 70 70 THR CA C 67.200 0.300 1 285 70 70 THR CB C 68.500 0.300 1 286 70 70 THR C C 176.400 0.300 1 287 71 71 ALA N N 124.020 0.300 1 288 71 71 ALA H H 7.750 0.030 1 289 71 71 ALA CA C 54.700 0.300 1 290 71 71 ALA CB C 19.550 0.300 1 291 71 71 ALA C C 178.200 0.300 1 292 72 72 LEU N N 117.070 0.300 1 293 72 72 LEU H H 8.230 0.030 1 294 72 72 LEU CA C 57.800 0.300 1 295 72 72 LEU CB C 42.150 0.300 1 296 72 72 LEU C C 178.900 0.300 1 297 73 73 LEU N N 117.270 0.300 1 298 73 73 LEU H H 7.830 0.030 1 299 73 73 LEU CA C 57.300 0.300 1 300 73 73 LEU CB C 42.000 0.300 1 301 73 73 LEU C C 179.150 0.300 1 302 74 74 ALA N N 120.650 0.300 1 303 74 74 ALA H H 7.320 0.030 1 304 74 74 ALA CA C 54.400 0.300 1 305 74 74 ALA CB C 19.950 0.300 1 306 74 74 ALA C C 180.200 0.300 1 307 75 75 ILE N N 115.990 0.300 1 308 75 75 ILE H H 7.870 0.030 1 309 75 75 ILE CA C 63.200 0.300 1 310 75 75 ILE CB C 38.950 0.300 1 311 75 75 ILE C C 177.100 0.300 1 312 76 76 GLN N N 119.750 0.300 1 313 76 76 GLN H H 7.780 0.030 1 314 76 76 GLN CA C 56.200 0.300 1 315 76 76 GLN CB C 29.300 0.300 1 316 76 76 GLN C C 175.250 0.300 1 317 77 77 SER N N 122.020 0.300 1 318 77 77 SER H H 7.630 0.030 1 319 77 77 SER CA C 60.500 0.300 1 320 77 77 SER CB C 64.900 0.300 1 321 77 77 SER C C 178.600 0.300 1 stop_ save_ save_RDC_list_1 _Saveframe_category residual_dipolar_couplings loop_ _Sample_label $sample_1 $sample_1 $sample_1 $sample_aligned $sample_aligned $sample_aligned stop_ loop_ _Residual_dipolar_coupling_ID _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_atom_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_atom_name _Residual_dipolar_coupling_value _Software_label _Atom_one_mol_system_component_name _Atom_two_mol_system_component_name _Residual_dipolar_coupling_min_value _Residual_dipolar_coupling_max_value DCCA 7 GLY C 7 GLY CA -2.44 $NMRPipe ? ? . . DCCA 11 ASN C 11 ASN CA -3.06 $NMRView ? ? . . DCCA 12 LEU C 12 LEU CA 0.17 $NMRView ? ? . . DCCA 13 LEU C 13 LEU CA 2.11 $NMRView ? ? . . DCCA 14 GLN C 14 GLN CA -4.26 $NMRView ? ? . . DCCA 15 GLU C 15 GLU CA -0.62 $NMRView ? ? . . DCCA 16 TYR C 16 TYR CA 0.21 $NMRView ? ? . . DCCA 17 ALA C 17 ALA CA 0.81 $NMRView ? ? . . DCCA 18 GLN C 18 GLN CA -5.50 $NMRView ? ? . . DCCA 19 LYS C 19 LYS CA 0.81 $NMRView ? ? . . DCCA 20 MET C 20 MET CA -0.88 $NMRView ? ? . . DCCA 21 ASN C 21 ASN CA -1.82 $NMRView ? ? . . DCCA 22 TYR C 22 TYR CA -2.63 $NMRView ? ? . . DCCA 23 ALA C 23 ALA CA -0.23 $NMRView ? ? . . DCCA 25 PRO C 25 PRO CA -5.35 $NMRView ? ? . . DCCA 26 LEU C 26 LEU CA 0.20 $NMRView ? ? . . DCCA 27 TYR C 27 TYR CA -4.38 $NMRView ? ? . . DCCA 29 CYS C 29 CYS CA -2.74 $NMRView ? ? . . DCCA 30 GLN C 30 GLN CA -0.58 $NMRView ? ? . . DCCA 31 LYS C 31 LYS CA 1.35 $NMRView ? ? . . DCCA 32 VAL C 32 VAL CA -1.92 $NMRView ? ? . . DCCA 33 GLU C 33 GLU CA 2.71 $NMRView ? ? . . DCCA 36 GLY C 36 GLY CA 1.31 $NMRView ? ? . . DCCA 37 ARG C 37 ARG CA -0.64 $NMRView ? ? . . DCCA 38 VAL C 38 VAL CA 0.01 $NMRView ? ? . . DCCA 39 THR C 39 THR CA -4.67 $NMRView ? ? . . DCCA 40 GLN C 40 GLN CA 0.61 $NMRView ? ? . . DCCA 41 PHE C 41 PHE CA -4.01 $NMRView ? ? . . DCCA 42 THR C 42 THR CA 1.32 $NMRView ? ? . . DCCA 43 CYS C 43 CYS CA -3.82 $NMRView ? ? . . DCCA 44 THR C 44 THR CA 1.64 $NMRView ? ? . . DCCA 45 VAL C 45 VAL CA -1.99 $NMRView ? ? . . DCCA 46 GLU C 46 GLU CA -1.74 $NMRView ? ? . . DCCA 47 ILE C 47 ILE CA -2.11 $NMRView ? ? . . DCCA 48 GLY C 48 GLY CA 1.25 $NMRView ? ? . . DCCA 49 GLY C 49 GLY CA 2.01 $NMRView ? ? . . DCCA 50 ILE C 50 ILE CA -3.05 $NMRView ? ? . . DCCA 51 LYS C 51 LYS CA 0.30 $NMRView ? ? . . DCCA 52 TYR C 52 TYR CA -4.61 $NMRView ? ? . . DCCA 53 THR C 53 THR CA 1.25 $NMRView ? ? . . DCCA 54 GLY C 54 GLY CA -0.43 $NMRView ? ? . . DCCA 55 ALA C 55 ALA CA -4.38 $NMRView ? ? . . DCCA 56 ALA C 56 ALA CA 0.01 $NMRView ? ? . . DCCA 57 THR C 57 THR CA -3.65 $NMRView ? ? . . DCCA 58 ARG C 58 ARG CA 1.39 $NMRView ? ? . . DCCA 59 THR C 59 THR CA -1.45 $NMRView ? ? . . DCCA 60 LYS C 60 LYS CA -2.85 $NMRView ? ? . . DCCA 61 LYS C 61 LYS CA -1.43 $NMRView ? ? . . DCCA 62 ASP C 62 ASP CA 0.37 $NMRView ? ? . . DCCA 63 ALA C 63 ALA CA -1.63 $NMRView ? ? . . DCCA 64 GLU C 64 GLU CA -4.16 $NMRView ? ? . . DCCA 65 ILE C 65 ILE CA 1.53 $NMRView ? ? . . DCCA 66 SER C 66 SER CA -1.30 $NMRView ? ? . . DCCA 67 ALA C 67 ALA CA -2.50 $NMRView ? ? . . DCCA 68 GLY C 68 GLY CA -1.76 $NMRView ? ? . . DCCA 69 ARG C 69 ARG CA 0.93 $NMRView ? ? . . DCCA 70 THR C 70 THR CA -1.17 $NMRView ? ? . . DCCA 71 ALA C 71 ALA CA -3.32 $NMRView ? ? . . DCCA 72 LEU C 72 LEU CA 0.04 $NMRView ? ? . . DCCA 73 LEU C 73 LEU CA -0.65 $NMRView ? ? . . DCCA 74 ALA C 74 ALA CA -1.35 $NMRView ? ? . . DCCA 75 ILE C 75 ILE CA -2.08 $NMRView ? ? . . DCCA 76 GLN C 76 GLN CA 0.18 $NMRView ? ? . . DCAHA 11 ASN CA 11 ASN HA 9.78 $NMRView ? ? . . DCAHA 12 LEU CA 12 LEU HA -7.58 $NMRView ? ? . . DCAHA 13 LEU CA 13 LEU HA -19.48 $NMRView ? ? . . DCAHA 15 GLU CA 15 GLU HA 29.25 $NMRView ? ? . . DCAHA 16 TYR CA 16 TYR HA -40.68 $NMRView ? ? . . DCAHA 17 ALA CA 17 ALA HA 10.85 $NMRView ? ? . . DCAHA 18 GLN CA 18 GLN HA 4.55 $NMRView ? ? . . DCAHA 19 LYS CA 19 LYS HA 14.87 $NMRView ? ? . . DCAHA 20 MET CA 20 MET HA -42.76 $NMRView ? ? . . DCAHA 21 ASN CA 21 ASN HA 15.66 $NMRView ? ? . . DCAHA 22 TYR CA 22 TYR HA 12.70 $NMRView ? ? . . DCAHA 23 ALA CA 23 ALA HA 29.53 $NMRView ? ? . . DCAHA 25 PRO CA 25 PRO HA 15.90 $NMRView ? ? . . DCAHA 26 LEU CA 26 LEU HA 20.33 $NMRView ? ? . . DCAHA 27 TYR CA 27 TYR HA 8.17 $NMRView ? ? . . DCAHA 29 CYS CA 29 CYS HA 1.64 $NMRView ? ? . . DCAHA 30 GLN CA 30 GLN HA 1.16 $NMRView ? ? . . DCAHA 31 LYS CA 31 LYS HA -4.99 $NMRView ? ? . . DCAHA 32 VAL CA 32 VAL HA -4.54 $NMRView ? ? . . DCAHA 33 GLU CA 33 GLU HA 5.84 $NMRView ? ? . . DCAHA 37 ARG CA 37 ARG HA 2.11 $NMRView ? ? . . DCAHA 38 VAL CA 38 VAL HA 0.78 $NMRView ? ? . . DCAHA 39 THR CA 39 THR HA 17.41 $NMRView ? ? . . DCAHA 40 GLN CA 40 GLN HA 12.81 $NMRView ? ? . . DCAHA 41 PHE CA 41 PHE HA -7.46 $NMRView ? ? . . DCAHA 42 THR CA 42 THR HA -5.39 $NMRView ? ? . . DCAHA 43 CYS CA 43 CYS HA -15.40 $NMRView ? ? . . DCAHA 44 THR CA 44 THR HA -2.41 $NMRView ? ? . . DCAHA 45 VAL CA 45 VAL HA 0.67 $NMRView ? ? . . DCAHA 46 GLU CA 46 GLU HA 23.39 $NMRView ? ? . . DCAHA 47 ILE CA 47 ILE HA 27.04 $NMRView ? ? . . DCAHA 50 ILE CA 50 ILE HA 17.96 $NMRView ? ? . . DCAHA 51 LYS CA 51 LYS HA 32.52 $NMRView ? ? . . DCAHA 52 TYR CA 52 TYR HA 28.20 $NMRView ? ? . . DCAHA 53 THR CA 53 THR HA 11.83 $NMRView ? ? . . DCAHA 55 ALA CA 55 ALA HA 2.12 $NMRView ? ? . . DCAHA 56 ALA CA 56 ALA HA 22.33 $NMRView ? ? . . DCAHA 57 THR CA 57 THR HA 19.97 $NMRView ? ? . . DCAHA 58 ARG CA 58 ARG HA -0.49 $NMRView ? ? . . DCAHA 59 THR CA 59 THR HA -42.28 $NMRView ? ? . . DCAHA 61 LYS CA 61 LYS HA 3.01 $NMRView ? ? . . DCAHA 62 ASP CA 62 ASP HA -22.07 $NMRView ? ? . . DCAHA 63 ALA CA 63 ALA HA 13.70 $NMRView ? ? . . DCAHA 64 GLU CA 64 GLU HA 8.35 $NMRView ? ? . . DCAHA 65 ILE CA 65 ILE HA -2.23 $NMRView ? ? . . DCAHA 66 SER CA 66 SER HA -21.22 $NMRView ? ? . . DCAHA 67 ALA CA 67 ALA HA 29.22 $NMRView ? ? . . DCAHA 69 ARG CA 69 ARG HA -11.78 $NMRView ? ? . . DCAHA 70 THR CA 70 THR HA 7.56 $NMRView ? ? . . DCAHA 71 ALA CA 71 ALA HA 17.11 $NMRView ? ? . . DCAHA 72 LEU CA 72 LEU HA 0.84 $NMRView ? ? . . DCAHA 73 LEU CA 73 LEU HA -18.09 $NMRView ? ? . . DCAHA 74 ALA CA 74 ALA HA 18.16 $NMRView ? ? . . DCAHA 75 ILE CA 75 ILE HA 7.50 $NMRView ? ? . . DCAHA 76 GLN CA 76 GLN HA 6.53 $NMRView ? ? . . DHN 8 LEU H 8 LEU N -0.06 $NMRView ? ? . . DHN 12 LEU H 12 LEU N 2.08 $NMRView ? ? . . DHN 13 LEU H 13 LEU N 14.10 $NMRView ? ? . . DHN 14 GLN H 14 GLN N 2.99 $NMRView ? ? . . DHN 15 GLU H 15 GLU N -1.48 $NMRView ? ? . . DHN 16 TYR H 16 TYR N 7.63 $NMRView ? ? . . DHN 17 ALA H 17 ALA N 10.11 $NMRView ? ? . . DHN 18 GLN H 18 GLN N 0.30 $NMRView ? ? . . DHN 19 LYS H 19 LYS N -0.67 $NMRView ? ? . . DHN 20 MET H 20 MET N 12.70 $NMRView ? ? . . DHN 21 ASN H 21 ASN N -6.77 $NMRView ? ? . . DHN 22 TYR H 22 TYR N -4.59 $NMRView ? ? . . DHN 23 ALA H 23 ALA N -12.83 $NMRView ? ? . . DHN 24 ILE H 24 ILE N -8.06 $NMRView ? ? . . DHN 26 LEU H 26 LEU N -2.55 $NMRView ? ? . . DHN 27 TYR H 27 TYR N -12.20 $NMRView ? ? . . DHN 28 GLN H 28 GLN N 2.46 $NMRView ? ? . . DHN 30 GLN H 30 GLN N 4.32 $NMRView ? ? . . DHN 31 LYS H 31 LYS N -0.81 $NMRView ? ? . . DHN 32 VAL H 32 VAL N -1.57 $NMRView ? ? . . DHN 33 GLU H 33 GLU N -2.10 $NMRView ? ? . . DHN 34 THR H 34 THR N -4.53 $NMRView ? ? . . DHN 37 ARG H 37 ARG N -3.02 $NMRView ? ? . . DHN 38 VAL H 38 VAL N -6.48 $NMRView ? ? . . DHN 39 THR H 39 THR N -0.18 $NMRView ? ? . . DHN 40 GLN H 40 GLN N -2.32 $NMRView ? ? . . DHN 41 PHE H 41 PHE N -0.11 $NMRView ? ? . . DHN 42 THR H 42 THR N 3.80 $NMRView ? ? . . DHN 43 CYS H 43 CYS N 1.97 $NMRView ? ? . . DHN 44 THR H 44 THR N 3.07 $NMRView ? ? . . DHN 45 VAL H 45 VAL N -7.73 $NMRView ? ? . . DHN 46 GLU H 46 GLU N -1.88 $NMRView ? ? . . DHN 47 ILE H 47 ILE N -17.65 $NMRView ? ? . . DHN 48 GLY H 48 GLY N -11.70 $NMRView ? ? . . DHN 49 GLY H 49 GLY N -0.10 $NMRView ? ? . . DHN 50 ILE H 50 ILE N -8.87 $NMRView ? ? . . DHN 51 LYS H 51 LYS N -12.62 $NMRView ? ? . . DHN 52 TYR H 52 TYR N -14.38 $NMRView ? ? . . DHN 53 THR H 53 THR N -1.74 $NMRView ? ? . . DHN 54 GLY H 54 GLY N 3.40 $NMRView ? ? . . DHN 55 ALA H 55 ALA N -3.53 $NMRView ? ? . . DHN 56 ALA H 56 ALA N -15.17 $NMRView ? ? . . DHN 57 THR H 57 THR N -6.14 $NMRView ? ? . . DHN 58 ARG H 58 ARG N 2.31 $NMRView ? ? . . DHN 59 THR H 59 THR N 13.96 $NMRView ? ? . . DHN 60 LYS H 60 LYS N 16.23 $NMRView ? ? . . DHN 61 LYS H 61 LYS N 15.63 $NMRView ? ? . . DHN 62 ASP H 62 ASP N 21.52 $NMRView ? ? . . DHN 63 ALA H 63 ALA N 17.13 $NMRView ? ? . . DHN 64 GLU H 64 GLU N 10.99 $NMRView ? ? . . DHN 65 ILE H 65 ILE N 16.67 $NMRView ? ? . . DHN 66 SER H 66 SER N 21.76 $NMRView ? ? . . DHN 67 ALA H 67 ALA N 13.31 $NMRView ? ? . . DHN 68 GLY H 68 GLY N 14.32 $NMRView ? ? . . DHN 69 ARG H 69 ARG N 20.39 $NMRView ? ? . . DHN 70 THR H 70 THR N 19.85 $NMRView ? ? . . DHN 71 ALA H 71 ALA N 13.28 $NMRView ? ? . . DHN 72 LEU H 72 LEU N 17.12 $NMRView ? ? . . DHN 73 LEU H 73 LEU N 22.92 $NMRView ? ? . . DHN 74 ALA H 74 ALA N 15.24 $NMRView ? ? . . DHN 75 ILE H 75 ILE N 13.53 $NMRView ? ? . . DHN 76 GLN H 76 GLN N 12.26 $NMRView ? ? . . DHN 77 SER H 77 SER N 0.57 $NMRView ? ? . . stop_ _Details . _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _Text_data_format . _Text_data . save_