data_17182 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Weak Substrate Binding to Transport Proteins Studied by NMR ; _BMRB_accession_number 17182 _BMRB_flat_file_name bmr17182.str _Entry_type original _Submission_date 2010-09-10 _Accession_date 2010-09-10 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Spooner Paul J.R. . 2 O'Reilly W. J. . 3 Homans Steven W. . 4 Rutherford Nicholas G. . 5 Henderson Peter J.F. . 6 Watts Anthony . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count chemical_rates 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-11-10 original author . stop_ _Original_release_date 2010-11-10 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Weak Substrate Binding to Transport Proteins Studied by NMR' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 9826601 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Spooner Paul J.R. . 2 O'Reilly W. J. . 3 Homans Steven W. . 4 Rutherford Nicholas G. . 5 Henderson Peter J.F. . 6 Watts Anthony . . stop_ _Journal_abbreviation 'Biophys. J.' _Journal_volume 75 _Journal_issue 6 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2794 _Page_last 2800 _Year 1998 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'FucP/arabinose complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label FucP $FucP arabinose $ARA stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_FucP _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common FucP _Molecular_mass 47545 _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 438 _Mol_residue_sequence ; MGNTSIQTQSYRAVDKDAGQ SRSYIIPFALLCSLFFLWAV ANNLNDILLPQFQQAFTLTN FQAGLIQSAFYFGYFIIPIP AGILMKKLSYKAGIITGLFL YALGAALFWPAAEIMNYTLF LVGLFIIAAGLGCLETAANP FVTVLGPESSGHFRLNLAQT FNSFGAIIAVVFGQSLILSN VPHQSQDVLDKMSPEQLSAY KHSLVLSVQTPYMIIVAIVL LVALLIMLTKFPALQSDNHS DAKQGSFSASLSRLARIRHW RWAVLAQFCYVGAQTACWSY LIRYAVEEIPGMTAGFAANY LTGTMVCFFIGRFTGTWLIS RFAPHKVLAAYALIAMALCL ISAFAGGHVGLIALTLCSAF MSIQYPTIFSLGIKNLGQDT KYGSSFIVMTIIGGGIVTPV MGFVSDAAGNIPTAELIPAL CFAVIFIFARFRSQTATN ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLY 3 ASN 4 THR 5 SER 6 ILE 7 GLN 8 THR 9 GLN 10 SER 11 TYR 12 ARG 13 ALA 14 VAL 15 ASP 16 LYS 17 ASP 18 ALA 19 GLY 20 GLN 21 SER 22 ARG 23 SER 24 TYR 25 ILE 26 ILE 27 PRO 28 PHE 29 ALA 30 LEU 31 LEU 32 CYS 33 SER 34 LEU 35 PHE 36 PHE 37 LEU 38 TRP 39 ALA 40 VAL 41 ALA 42 ASN 43 ASN 44 LEU 45 ASN 46 ASP 47 ILE 48 LEU 49 LEU 50 PRO 51 GLN 52 PHE 53 GLN 54 GLN 55 ALA 56 PHE 57 THR 58 LEU 59 THR 60 ASN 61 PHE 62 GLN 63 ALA 64 GLY 65 LEU 66 ILE 67 GLN 68 SER 69 ALA 70 PHE 71 TYR 72 PHE 73 GLY 74 TYR 75 PHE 76 ILE 77 ILE 78 PRO 79 ILE 80 PRO 81 ALA 82 GLY 83 ILE 84 LEU 85 MET 86 LYS 87 LYS 88 LEU 89 SER 90 TYR 91 LYS 92 ALA 93 GLY 94 ILE 95 ILE 96 THR 97 GLY 98 LEU 99 PHE 100 LEU 101 TYR 102 ALA 103 LEU 104 GLY 105 ALA 106 ALA 107 LEU 108 PHE 109 TRP 110 PRO 111 ALA 112 ALA 113 GLU 114 ILE 115 MET 116 ASN 117 TYR 118 THR 119 LEU 120 PHE 121 LEU 122 VAL 123 GLY 124 LEU 125 PHE 126 ILE 127 ILE 128 ALA 129 ALA 130 GLY 131 LEU 132 GLY 133 CYS 134 LEU 135 GLU 136 THR 137 ALA 138 ALA 139 ASN 140 PRO 141 PHE 142 VAL 143 THR 144 VAL 145 LEU 146 GLY 147 PRO 148 GLU 149 SER 150 SER 151 GLY 152 HIS 153 PHE 154 ARG 155 LEU 156 ASN 157 LEU 158 ALA 159 GLN 160 THR 161 PHE 162 ASN 163 SER 164 PHE 165 GLY 166 ALA 167 ILE 168 ILE 169 ALA 170 VAL 171 VAL 172 PHE 173 GLY 174 GLN 175 SER 176 LEU 177 ILE 178 LEU 179 SER 180 ASN 181 VAL 182 PRO 183 HIS 184 GLN 185 SER 186 GLN 187 ASP 188 VAL 189 LEU 190 ASP 191 LYS 192 MET 193 SER 194 PRO 195 GLU 196 GLN 197 LEU 198 SER 199 ALA 200 TYR 201 LYS 202 HIS 203 SER 204 LEU 205 VAL 206 LEU 207 SER 208 VAL 209 GLN 210 THR 211 PRO 212 TYR 213 MET 214 ILE 215 ILE 216 VAL 217 ALA 218 ILE 219 VAL 220 LEU 221 LEU 222 VAL 223 ALA 224 LEU 225 LEU 226 ILE 227 MET 228 LEU 229 THR 230 LYS 231 PHE 232 PRO 233 ALA 234 LEU 235 GLN 236 SER 237 ASP 238 ASN 239 HIS 240 SER 241 ASP 242 ALA 243 LYS 244 GLN 245 GLY 246 SER 247 PHE 248 SER 249 ALA 250 SER 251 LEU 252 SER 253 ARG 254 LEU 255 ALA 256 ARG 257 ILE 258 ARG 259 HIS 260 TRP 261 ARG 262 TRP 263 ALA 264 VAL 265 LEU 266 ALA 267 GLN 268 PHE 269 CYS 270 TYR 271 VAL 272 GLY 273 ALA 274 GLN 275 THR 276 ALA 277 CYS 278 TRP 279 SER 280 TYR 281 LEU 282 ILE 283 ARG 284 TYR 285 ALA 286 VAL 287 GLU 288 GLU 289 ILE 290 PRO 291 GLY 292 MET 293 THR 294 ALA 295 GLY 296 PHE 297 ALA 298 ALA 299 ASN 300 TYR 301 LEU 302 THR 303 GLY 304 THR 305 MET 306 VAL 307 CYS 308 PHE 309 PHE 310 ILE 311 GLY 312 ARG 313 PHE 314 THR 315 GLY 316 THR 317 TRP 318 LEU 319 ILE 320 SER 321 ARG 322 PHE 323 ALA 324 PRO 325 HIS 326 LYS 327 VAL 328 LEU 329 ALA 330 ALA 331 TYR 332 ALA 333 LEU 334 ILE 335 ALA 336 MET 337 ALA 338 LEU 339 CYS 340 LEU 341 ILE 342 SER 343 ALA 344 PHE 345 ALA 346 GLY 347 GLY 348 HIS 349 VAL 350 GLY 351 LEU 352 ILE 353 ALA 354 LEU 355 THR 356 LEU 357 CYS 358 SER 359 ALA 360 PHE 361 MET 362 SER 363 ILE 364 GLN 365 TYR 366 PRO 367 THR 368 ILE 369 PHE 370 SER 371 LEU 372 GLY 373 ILE 374 LYS 375 ASN 376 LEU 377 GLY 378 GLN 379 ASP 380 THR 381 LYS 382 TYR 383 GLY 384 SER 385 SER 386 PHE 387 ILE 388 VAL 389 MET 390 THR 391 ILE 392 ILE 393 GLY 394 GLY 395 GLY 396 ILE 397 VAL 398 THR 399 PRO 400 VAL 401 MET 402 GLY 403 PHE 404 VAL 405 SER 406 ASP 407 ALA 408 ALA 409 GLY 410 ASN 411 ILE 412 PRO 413 THR 414 ALA 415 GLU 416 LEU 417 ILE 418 PRO 419 ALA 420 LEU 421 CYS 422 PHE 423 ALA 424 VAL 425 ILE 426 PHE 427 ILE 428 PHE 429 ALA 430 ARG 431 PHE 432 ARG 433 SER 434 GLN 435 THR 436 ALA 437 THR 438 ASN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 3O7P "Crystal Structure Of The E.coli Fucose:proton Symporter, Fucp (n162a)" 100.00 438 99.77 99.77 0.00e+00 PDB 3O7Q "Crystal Structure Of A Major Facilitator Superfamily (mfs) Transporter, Fucp, In The Outward Conformation" 100.00 438 100.00 100.00 0.00e+00 DBJ BAB37084 "fucose permease [Escherichia coli O157:H7 str. Sakai]" 100.00 438 99.77 99.77 0.00e+00 DBJ BAE76873 "L-fucose transporter [Escherichia coli str. K12 substr. W3110]" 100.00 438 100.00 100.00 0.00e+00 DBJ BAG78585 "fucose transport protein [Escherichia coli SE11]" 100.00 438 100.00 100.00 0.00e+00 DBJ BAI27062 "L-fucose transporter [Escherichia coli O26:H11 str. 11368]" 100.00 438 100.00 100.00 0.00e+00 DBJ BAI32091 "L-fucose transporter [Escherichia coli O103:H2 str. 12009]" 100.00 438 100.00 100.00 0.00e+00 EMBL CAA33126 "unnamed protein product [Escherichia coli K-12]" 100.00 438 100.00 100.00 0.00e+00 EMBL CAP77234 "L-fucose-proton symporter [Escherichia coli LF82]" 100.00 438 99.77 99.77 0.00e+00 EMBL CAQ99729 "L-fucose transporter [Escherichia coli IAI1]" 100.00 438 100.00 100.00 0.00e+00 EMBL CAR04311 "L-fucose transporter [Escherichia coli S88]" 100.00 438 99.54 99.77 0.00e+00 EMBL CAR09414 "L-fucose transporter [Escherichia coli ED1a]" 100.00 438 99.77 99.77 0.00e+00 GB AAB40451 "L-fucose permease [Escherichia coli str. K-12 substr. MG1655]" 100.00 438 100.00 100.00 0.00e+00 GB AAC75843 "L-fucose transporter [Escherichia coli str. K-12 substr. MG1655]" 100.00 438 100.00 100.00 0.00e+00 GB AAG57915 "fucose permease [Escherichia coli O157:H7 str. EDL933]" 100.00 438 99.77 99.77 0.00e+00 GB AAN44303 "fucose permease [Shigella flexneri 2a str. 301]" 100.00 438 99.54 99.54 0.00e+00 GB AAN81817 "L-fucose permease [Escherichia coli CFT073]" 100.00 438 99.54 99.54 0.00e+00 REF NP_311688 "L-fucose transporter [Escherichia coli O157:H7 str. Sakai]" 100.00 438 99.77 99.77 0.00e+00 REF NP_417281 "L-fucose transporter [Escherichia coli str. K-12 substr. MG1655]" 100.00 438 100.00 100.00 0.00e+00 REF NP_708596 "L-fucose transporter [Shigella flexneri 2a str. 301]" 100.00 438 99.54 99.54 0.00e+00 REF WP_000528574 "L-fucose transporter [Escherichia coli]" 100.00 438 99.77 99.77 0.00e+00 REF WP_000528575 "L-fucose-proton symporter [Escherichia coli]" 100.00 438 99.77 100.00 0.00e+00 SP P11551 "RecName: Full=L-fucose-proton symporter; AltName: Full=6-deoxy-L-galactose permease; AltName: Full=L-fucose permease" 100.00 438 100.00 100.00 0.00e+00 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain _Variant $FucP 'E. coli' 562 Bacteria . Escherichia coli AR120 pFG7 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $FucP 'purified from the natural source' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type membrane _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $ARA 150.13 ug . . [U-13C] $FucP 9.5 mg . . 'natural abundance' D-fucose . ug 164.2 492.5 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software _Saveframe_category software _Name software _Version . loop_ _Vendor _Address _Electronic_address na . . stop_ loop_ _Task na stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model MSL _Field_strength 400 _Details 'proton resonance given; 13C resonance of 100 MHz documented' save_ ############################# # NMR applied experiments # ############################# save_13C_CP_MAS_1 _Saveframe_category NMR_applied_experiment _Experiment_name '13C CP MAS' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.5 . pH pressure ambient . atm temperature 275 . K stop_ save_