data_17440 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structure of trans-Resveratrol in complex with the cardiac regulatory protein Troponin C ; _BMRB_accession_number 17440 _BMRB_flat_file_name bmr17440.str _Entry_type original _Submission_date 2011-02-02 _Accession_date 2011-02-02 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sykes Brian D. . 2 Pineda-Sanabria Sandra E. . 3 Robertson Ian M. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 9 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-03-03 update BMRB 'Update entry citation' 2011-02-24 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structure of trans-Resveratrol in Complex with the Cardiac Regulatory Protein Troponin C.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 21226534 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Pineda-Sanabria Sandra E. . 2 Robertson Ian M. . 3 Sykes Brian D. . stop_ _Journal_abbreviation Biochemistry _Journal_name_full Biochemistry _Journal_volume 50 _Journal_issue 8 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1309 _Page_last 1320 _Year 2011 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'trans-Resveratrol in complex with the cardiac regulatory protein Troponin C' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label CcTnC $CcTnC RESVERATROL $STL 'Calcium ion' $CA stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_CcTnC _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common CcTnC _Molecular_mass 8369.260 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 72 _Mol_residue_sequence ; MGKSEEELSDLFRMFDKNAD GYIDLDELKIMLQATGETIT EDDIEELMKDGDKNNDGRID YDEFLEFMKGVE ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 MET 2 2 GLY 3 3 LYS 4 4 SER 5 5 GLU 6 6 GLU 7 7 GLU 8 8 LEU 9 9 SER 10 10 ASP 11 11 LEU 12 12 PHE 13 13 ARG 14 14 MET 15 15 PHE 16 16 ASP 17 17 LYS 18 18 ASN 19 19 ALA 20 20 ASP 21 21 GLY 22 22 TYR 23 23 ILE 24 24 ASP 25 25 LEU 26 26 ASP 27 27 GLU 28 28 LEU 29 29 LYS 30 30 ILE 31 31 MET 32 32 LEU 33 33 GLN 34 34 ALA 35 35 THR 36 36 GLY 37 37 GLU 38 38 THR 39 39 ILE 40 40 THR 41 41 GLU 42 42 ASP 43 43 ASP 44 44 ILE 45 45 GLU 46 46 GLU 47 47 LEU 48 48 MET 49 49 LYS 50 50 ASP 51 51 GLY 52 52 ASP 53 53 LYS 54 54 ASN 55 55 ASN 56 56 ASP 57 57 GLY 58 58 ARG 59 59 ILE 60 60 ASP 61 61 TYR 62 62 ASP 63 63 GLU 64 64 PHE 65 65 LEU 66 66 GLU 67 67 PHE 68 68 MET 69 69 LYS 70 70 GLY 71 71 VAL 72 72 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-09-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15385 F104W 98.61 161 98.59 100.00 1.33e-38 BMRB 15388 F153W 98.61 161 98.59 100.00 1.33e-38 BMRB 15400 F153(FTR) 98.61 161 98.59 98.59 5.73e-38 BMRB 15427 F104(FTR) 98.61 161 98.59 100.00 1.33e-38 BMRB 16752 TnC 98.61 161 97.18 100.00 4.87e-38 BMRB 17243 cCTnC 100.00 72 100.00 100.00 2.09e-40 BMRB 19816 cCTnC 100.00 72 100.00 100.00 2.09e-40 BMRB 19817 cCTnC 100.00 72 98.61 98.61 2.69e-39 BMRB 25120 cTnC 98.61 161 100.00 100.00 3.84e-39 PDB 1AJ4 "Structure Of Calcium-Saturated Cardiac Troponin C, Nmr, 1 Structure" 98.61 161 97.18 100.00 2.66e-38 PDB 1DTL "Crystal Structure Of Calcium-Saturated (3ca2+) Cardiac Troponin C Complexed With The Calcium Sensitizer Bepridil At 2.15 A Reso" 98.61 161 98.59 100.00 1.60e-38 PDB 1FI5 "Nmr Structure Of The C Terminal Domain Of Cardiac Troponin C Bound To The N Terminal Domain Of Cardiac Troponin I." 98.61 81 97.18 100.00 1.16e-38 PDB 1IH0 "Structure Of The C-Domain Of Human Cardiac Troponin C In Complex With Ca2+ Sensitizer Emd 57033" 97.22 71 98.57 100.00 2.96e-38 PDB 1J1D "Crystal Structure Of The 46kda Domain Of Human Cardiac Troponin In The Ca2+ Saturated Form" 98.61 161 98.59 100.00 1.60e-38 PDB 1J1E "Crystal Structure Of The 52kda Domain Of Human Cardiac Troponin In The Ca2+ Saturated Form" 98.61 161 98.59 100.00 1.60e-38 PDB 1LA0 "Solution Structure Of Calcium Saturated Cardiac Troponin C In The Troponin C-Troponin I Complex" 98.61 161 97.18 100.00 4.87e-38 PDB 1OZS "C-Domain Of Human Cardiac Troponin C In Complex With The Inhibitory Region Of Human Cardiac Troponin I" 98.61 73 98.59 100.00 4.50e-39 PDB 1SBJ "Nmr Structure Of The Mg2+-Loaded C Terminal Domain Of Cardiac Troponin C Bound To The N Terminal Domain Of Cardiac Troponin I" 98.61 81 97.18 100.00 1.16e-38 PDB 1SCV "Nmr Structure Of The C Terminal Domain Of Cardiac Troponin C Bound To The N Terminal Domain Of Cardiac Troponin I" 98.61 81 97.18 100.00 1.16e-38 PDB 2JT0 "Solution Structure Of F104w Cardiac Troponin C" 98.61 161 98.59 100.00 1.33e-38 PDB 2JT3 "Solution Structure Of F153w Cardiac Troponin C" 98.61 161 98.59 100.00 1.33e-38 PDB 2JT8 "Solution Structure Of The F153-To-5-Flurotryptophan Mutant Of Human Cardiac Troponin C" 98.61 161 98.59 98.59 5.73e-38 PDB 2JTZ "Solution Structure And Chemical Shift Assignments Of The F104-To-5-Flurotryptophan Mutant Of Cardiac Troponin C" 98.61 161 98.59 98.59 5.73e-38 PDB 2KDH "The Solution Structure Of Human Cardiac Troponin C In Complex With The Green Tea Polyphenol; (-)- Epigallocatechin-3-Gallate" 100.00 72 100.00 100.00 2.09e-40 PDB 2L98 "Structure Of Trans-Resveratrol In Complex With The Cardiac Regulatory Protein Troponin C" 100.00 72 100.00 100.00 2.09e-40 PDB 2MLE "Nmr Structure Of The C-domain Of Troponin C Bound To The Anchoring Region Of Troponin I" 100.00 72 100.00 100.00 2.09e-40 PDB 2MLF "Nmr Structure Of The Dilated Cardiomyopathy Mutation G159d In Troponin C Bound To The Anchoring Region Of Troponin I" 100.00 72 98.61 98.61 2.69e-39 PDB 3CTN "Structure Of Calcium-Saturated Cardiac Troponin C, Nmr, 30 Structures" 98.61 76 97.18 100.00 1.37e-38 DBJ BAA02369 "cardiac troponin C [Gallus gallus]" 98.61 161 97.18 100.00 4.87e-38 DBJ BAG36483 "unnamed protein product [Homo sapiens]" 98.61 161 100.00 100.00 4.52e-39 EMBL CAA30736 "unnamed protein product [Homo sapiens]" 98.61 161 100.00 100.00 4.52e-39 EMBL CAG46663 "TNNC1 [Homo sapiens]" 98.61 161 100.00 100.00 4.52e-39 EMBL CAG46683 "TNNC1 [Homo sapiens]" 98.61 161 100.00 100.00 4.52e-39 GB AAA36772 "slow twitch skeletal/cardiac muscle troponin C [Homo sapiens]" 98.61 161 100.00 100.00 4.52e-39 GB AAA37492 "slow/cardiac troponin C, partial [Mus musculus]" 98.61 161 98.59 100.00 1.42e-38 GB AAA37493 "slow/cardiac troponin C [Mus musculus]" 98.61 161 98.59 100.00 1.42e-38 GB AAA48654 "slow muscle troponin C [Gallus gallus]" 98.61 161 97.18 100.00 4.87e-38 GB AAB91994 "cardiac ventricular troponin C [Homo sapiens]" 98.61 160 98.59 98.59 1.05e-36 PIR TPHUCC "troponin C, cardiac and slow skeletal muscle - human" 98.61 161 100.00 100.00 4.52e-39 PRF 1510257A "troponin C" 98.61 161 98.59 100.00 1.84e-38 PRF 750650A "troponin c,cardiac" 98.61 161 98.59 100.00 1.84e-38 REF NP_001029277 "troponin C, slow skeletal and cardiac muscles [Rattus norvegicus]" 98.61 161 98.59 100.00 1.42e-38 REF NP_001029523 "troponin C, slow skeletal and cardiac muscles [Bos taurus]" 98.61 161 98.59 100.00 1.84e-38 REF NP_001123715 "troponin C, slow skeletal and cardiac muscles [Sus scrofa]" 98.61 161 98.59 100.00 1.84e-38 REF NP_001272501 "troponin C, slow skeletal and cardiac muscles [Capra hircus]" 98.61 161 98.59 100.00 1.84e-38 REF NP_001291793 "troponin C type 1 (slow) [Ailuropoda melanoleuca]" 98.61 161 97.18 98.59 4.87e-38 SP P02591 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 98.61 161 100.00 100.00 4.52e-39 SP P09860 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 98.61 161 97.18 100.00 4.87e-38 SP P19123 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 98.61 161 98.59 100.00 1.42e-38 SP P63315 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 98.61 161 98.59 100.00 1.84e-38 SP P63316 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 98.61 161 100.00 100.00 4.52e-39 TPG DAA16908 "TPA: troponin C, slow skeletal and cardiac muscles [Bos taurus]" 98.61 161 98.59 100.00 1.84e-38 stop_ save_ ############# # Ligands # ############# save_STL _Saveframe_category ligand _Mol_type non-polymer _Name_common "STL (RESVERATROL)" _BMRB_code . _PDB_code STL _Molecular_mass 228.243 _Mol_charge 0 _Mol_paramagnetic no _Mol_aromatic yes _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Fri Feb 11 11:45:05 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons C1 C1 C . 0 . ? C2 C2 C . 0 . ? C3 C3 C . 0 . ? C4 C4 C . 0 . ? C5 C5 C . 0 . ? C6 C6 C . 0 . ? C7 C7 C . 0 . ? C8 C8 C . 0 . ? C9 C9 C . 0 . ? C10 C10 C . 0 . ? C11 C11 C . 0 . ? C12 C12 C . 0 . ? C13 C13 C . 0 . ? C14 C14 C . 0 . ? O1 O1 O . 0 . ? O2 O2 O . 0 . ? O3 O3 O . 0 . ? H2 H2 H . 0 . ? H4 H4 H . 0 . ? H6 H6 H . 0 . ? H7 H7 H . 0 . ? H8 H8 H . 0 . ? H10 H10 H . 0 . ? H11 H11 H . 0 . ? H13 H13 H . 0 . ? H14 H14 H . 0 . ? HO1 HO1 H . 0 . ? HO2 HO2 H . 0 . ? HO3 HO3 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name DOUB C1 C2 ? ? SING C1 C6 ? ? SING C1 O3 ? ? SING C2 C3 ? ? SING C2 H2 ? ? DOUB C3 C4 ? ? SING C3 O2 ? ? SING C4 C5 ? ? SING C4 H4 ? ? DOUB C5 C6 ? ? SING C5 C7 ? ? SING C6 H6 ? ? DOUB C7 C8 ? ? SING C7 H7 ? ? SING C8 C9 ? ? SING C8 H8 ? ? DOUB C9 C10 ? ? SING C9 C14 ? ? SING C10 C11 ? ? SING C10 H10 ? ? DOUB C11 C12 ? ? SING C11 H11 ? ? SING C12 C13 ? ? SING C12 O1 ? ? DOUB C13 C14 ? ? SING C13 H13 ? ? SING C14 H14 ? ? SING O1 HO1 ? ? SING O2 HO2 ? ? SING O3 HO3 ? ? stop_ _Mol_thiol_state 'not present' _Sequence_homology_query_date . save_ save_CA _Saveframe_category ligand _Mol_type non-polymer _Name_common "CA (CALCIUM ION)" _BMRB_code . _PDB_code CA _Molecular_mass 40.078 _Mol_charge 2 _Mol_paramagnetic no _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Fri Feb 11 11:43:37 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons CA CA CA . 2 . ? stop_ _Mol_thiol_state 'not present' _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Gene_mnemonic $CcTnC Human 9606 Eukaryota Metazoa Homo sapiens TNNC1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $CcTnC 'recombinant technology' . . . 'Bl21 (de3) Plyss' pET3C $STL 'obtained from a vendor' . . . . . $CA 'obtained from a vendor' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $CcTnC 0.5 mM . . '[U-100% 13C; U-100% 15N]' $STL 2.0 mM . . 'natural abundance' $CA 10.0 mM . . 'natural abundance' TCEP . mM 6 8 'natural abundance' DSS 0.5 mM . . '[U-99% 2H]' 'potassium chloride' 100 mM . . 'natural abundance' imidazole 10 mM . . 'natural abundance' H2O 100 % . . 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $CcTnC 0.5 mM . . '[U-100% 13C; U-100% 15N]' $STL 2.0 mM . . 'natural abundance' $CA 10.0 mM . . 'natural abundance' TCEP . mM 6 8 'natural abundance' DSS 0.5 mM . . '[U-99% 2H]' 'potassium chloride' 100 mM . . 'natural abundance' imidazole 8 mM . . 'natural abundance' imidazole 2 mM . . [U-2H] D2O 100 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_VNMRJ _Saveframe_category software _Name VNMRJ _Version . loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task collection stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_X-PLOR_NIH _Saveframe_category software _Name 'X-PLOR NIH' _Version . loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement 'structure solution' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 500 _Details 'Equipped with triple-resonance probe with Z-pulsed field gradient' save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Unity _Field_strength 600 _Details 'Equipped with triple-resonance probe with Z-pulsed field gradient' save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 800 _Details 'Equipped with triple-resonance probe with Z-pulsed field gradient' save_ ############################# # NMR applied experiments # ############################# save_1D_1H_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1D 1H' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_aliphatic_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aliphatic' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_ROESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H ROESY' _Sample_label $sample_2 save_ save_2D_13C-15N_filtered_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 13C-15N filtered NOESY' _Sample_label $sample_1 save_ save_2D_13C_edited/filtered_NOESY-HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 13C edited/filtered NOESY-HSQC' _Sample_label $sample_1 save_ save_3D_13C_edited/filtered_HMQC-NOESY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 13C edited/filtered HMQC-NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.9 . pH pressure 1 . atm temperature 303 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.9 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H 1 'methyl protons' ppm 0 internal direct . . . 1.0 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-1H ROESY' stop_ loop_ _Sample_label $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name RESVERATROL _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 STL H10 H 7.461 . 1 2 1 1 STL H11 H 6.873 . 1 3 1 1 STL H13 H 6.889 . 1 4 1 1 STL H14 H 7.478 . 1 5 1 1 STL H2 H 6.314 . 1 6 1 1 STL H4 H 6.600 . 1 7 1 1 STL H6 H 6.600 . 1 8 1 1 STL H7 H 6.926 . 1 9 1 1 STL H8 H 7.111 . 1 stop_ save_