data_17464 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution Structure of Acidic Fibroblast Growth Factor Bound to 1,3,6-Naphthalenetrisulfonate: A Minimal Model for the Anti-tumoral Action of Suramins and Suradistas ; _BMRB_accession_number 17464 _BMRB_flat_file_name bmr17464.str _Entry_type original _Submission_date 2011-02-11 _Accession_date 2011-02-11 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lozano Rosa M. . 2 Jimenez M. A. . 3 Santoro Jorge . . 4 Rico Manuel . . 5 Gimenez-Gallego Guillermo . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count chemical_rates 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-06-06 original author . stop_ _Original_release_date 2012-06-06 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution Structure of Acidic Fibroblast Growth Factor Bound to 1,3,6-Naphthalenetrisulfonate: A Minimal Model for the Anti-tumoral Action of Suramins and Suradistas' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lozano Rosa M. . 2 Jimenez M. A. . 3 Santoro Jorge . . 4 Rico Manuel . . 5 Gimenez-Gallego Guillermo . . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 281 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 899 _Page_last 915 _Year 1998 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'FGF/NTS complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label FGF $FGF NTS $NTS stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic yes _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_FGF _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common FGF _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 132 _Mol_residue_sequence ; KKPKLLYCSNGGHFLRILPD GTVDGTRDRSDQHIQLQLSA ESVGEVYIKSTETGQYLAMD TDGLLYGSQTPNEECLFLER LEENHYNTYISKKHAEKNWF VGLKKNGSCKRGPRTHYGQK AILFLPLPVSSD ; loop_ _Residue_seq_code _Residue_label 1 LYS 2 LYS 3 PRO 4 LYS 5 LEU 6 LEU 7 TYR 8 CYS 9 SER 10 ASN 11 GLY 12 GLY 13 HIS 14 PHE 15 LEU 16 ARG 17 ILE 18 LEU 19 PRO 20 ASP 21 GLY 22 THR 23 VAL 24 ASP 25 GLY 26 THR 27 ARG 28 ASP 29 ARG 30 SER 31 ASP 32 GLN 33 HIS 34 ILE 35 GLN 36 LEU 37 GLN 38 LEU 39 SER 40 ALA 41 GLU 42 SER 43 VAL 44 GLY 45 GLU 46 VAL 47 TYR 48 ILE 49 LYS 50 SER 51 THR 52 GLU 53 THR 54 GLY 55 GLN 56 TYR 57 LEU 58 ALA 59 MET 60 ASP 61 THR 62 ASP 63 GLY 64 LEU 65 LEU 66 TYR 67 GLY 68 SER 69 GLN 70 THR 71 PRO 72 ASN 73 GLU 74 GLU 75 CYS 76 LEU 77 PHE 78 LEU 79 GLU 80 ARG 81 LEU 82 GLU 83 GLU 84 ASN 85 HIS 86 TYR 87 ASN 88 THR 89 TYR 90 ILE 91 SER 92 LYS 93 LYS 94 HIS 95 ALA 96 GLU 97 LYS 98 ASN 99 TRP 100 PHE 101 VAL 102 GLY 103 LEU 104 LYS 105 LYS 106 ASN 107 GLY 108 SER 109 CYS 110 LYS 111 ARG 112 GLY 113 PRO 114 ARG 115 THR 116 HIS 117 TYR 118 GLY 119 GLN 120 LYS 121 ALA 122 ILE 123 LEU 124 PHE 125 LEU 126 PRO 127 LEU 128 PRO 129 VAL 130 SER 131 SER 132 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-03-22 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15783 FGF-1 100.00 133 100.00 100.00 1.37e-92 BMRB 15960 FGF-1 100.00 133 100.00 100.00 1.37e-92 BMRB 16493 aFGF 100.00 132 100.00 100.00 1.20e-92 BMRB 16494 aFGF 100.00 132 100.00 100.00 1.20e-92 BMRB 16502 aFGF 100.00 132 100.00 100.00 1.20e-92 BMRB 17674 FGF1 97.73 130 100.00 100.00 1.84e-90 PDB 1AXM "Heparin-Linked Biologically-Active Dimer Of Fibroblast Growth Factor" 100.00 135 99.24 99.24 2.73e-91 PDB 1DJS "Ligand-binding Portion Of Fibroblast Growth Factor Receptor 2 In Complex With Fgf1" 100.00 135 99.24 99.24 2.73e-91 PDB 1DZC "High Resolution Structure Of Acidic Fibroblast Growth Factor. Mutant Fgf-4-ala-(23-154), 24 Nmr Structures" 96.97 131 100.00 100.00 6.19e-90 PDB 1DZD "High Resolution Structure Of Acidic Fibroblast Growth Factor (27-154), 24 Nmr Structures" 96.21 127 100.00 100.00 4.99e-89 PDB 1E0O "Crystal Structure Of A Ternary Fgf1-Fgfr2-Heparin Complex" 100.00 140 100.00 100.00 3.62e-92 PDB 1EVT "Crystal Structure Of Fgf1 In Complex With The Extracellular Ligand Binding Domain Of Fgf Receptor 1 (Fgfr1)" 100.00 134 100.00 100.00 1.31e-92 PDB 1HKN "A Complex Between Acidic Fibroblast Growth Factor And 5- Amino-2-Naphthalenesulfonate" 100.00 139 100.00 100.00 3.28e-92 PDB 1JQZ "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag." 100.00 146 100.00 100.00 6.54e-92 PDB 1JT3 "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Histidine Tag And Leu 73 Replaced By Val (L73v)" 100.00 146 99.24 100.00 1.26e-91 PDB 1JT4 "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag And Val 109 Replaced By Leu (v109l)" 100.00 146 99.24 100.00 2.17e-91 PDB 1JT5 "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag And Leu 73 Replaced By Val And Val 109 R" 100.00 146 98.48 100.00 5.75e-91 PDB 1JT7 "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag And Leu 44 Replaced By Phe And Leu 73 Re" 100.00 146 97.73 99.24 1.59e-90 PDB 1JTC "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag And Leu 44 Replaced By Phe (L44f)" 100.00 146 99.24 99.24 1.79e-91 PDB 1JY0 "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag And Cys 117 Replaced With Val (C117v)" 100.00 146 99.24 99.24 5.50e-91 PDB 1K5U "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag With His93 Replaced By Gly (H93g)" 100.00 146 99.24 99.24 1.93e-90 PDB 1K5V "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag With Asn106 Replaced By Gly (N106g)" 100.00 146 99.24 99.24 6.55e-91 PDB 1M16 "Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form With Amino Terminal His Tag And Leu 44 Replaced With Phe (L44f), Leu" 100.00 146 96.97 98.48 1.55e-89 PDB 1P63 "Human Acidic Fibroblast Growth Factor. 140 Amino Acid Form With Amino Terminal His Tag And Leu111 Replaced With Ile (L111i)" 100.00 144 99.24 100.00 1.17e-91 PDB 1PZZ "Crystal Structure Of Fgf-1, V51n Mutant" 100.00 146 99.24 99.24 7.80e-91 PDB 1Q03 "Crystal Structure Of Fgf-1, S50gV51G MUTANT" 100.00 146 98.48 98.48 5.33e-90 PDB 1Q04 "Crystal Structure Of Fgf-1, S50e/v51n" 100.00 146 98.48 98.48 2.86e-90 PDB 1RG8 "Human Acidic Fibroblast Growth Factor (Hafgf-1) At 1.10 Angstrom Resolution (140 Amino Acid Form)" 100.00 146 100.00 100.00 6.54e-92 PDB 1RML "Nmr Study Of Acid Fibroblast Growth Factor Bound To 1,3,6- Naphthalene Trisulphonate, 26 Structures" 100.00 155 100.00 100.00 1.26e-92 PDB 1RY7 "Crystal Structure Of The 3 Ig Form Of Fgfr3c In Complex With Fgf1" 100.00 155 100.00 100.00 1.26e-92 PDB 1YTO "Crystal Structure Of Gly19 Deletion Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 145 99.24 99.24 2.74e-89 PDB 1Z2V "Crystal Structure Of Glu60 Deletion Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 145 99.24 99.24 2.28e-89 PDB 1Z4S "Crystal Structure Of Gly19 And Glu60 Deletion Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 144 98.48 98.48 7.66e-87 PDB 2AFG "2.0 Angstrom X-Ray Structure Of Human Acidic Fibroblast Growth Factor" 100.00 140 100.00 100.00 3.62e-92 PDB 2AQZ "Crystal Structure Of Fgf-1, S17tN18TG19 DELETION MUTANT" 100.00 145 97.73 98.48 3.27e-88 PDB 2AXM "Heparin-Linked Biologically-Active Dimer Of Fibroblast Growth Factor" 100.00 135 100.00 100.00 1.70e-92 PDB 2ERM "Solution Structure Of A Biologically Active Human Fgf-1 Monomer, Complexed To A Hexasaccharide Heparin-Analogue" 100.00 139 100.00 100.00 3.28e-92 PDB 2HW9 "Crystal Structure Of Lys12cysCYS117VAL MUTANT OF HUMAN Acidic Fibroblast Growth Factor At 1.60 Angstrom Resolution" 100.00 146 98.48 98.48 2.13e-89 PDB 2HWA "Crystal Structure Of Lys12thrCYS117VAL MUTANT OF HUMAN Acidic Fibroblast Growth Factor At 1.65 Angstrom Resolution" 100.00 146 98.48 98.48 3.33e-90 PDB 2HWM "Crystal Structure Of Lys12valCYS117VAL MUTANT OF HUMAN Acidic Fibroblast Growth Factor At 1.60 Angstrom Resolution" 100.00 146 98.48 98.48 5.57e-90 PDB 2HZ9 "Crystal Structure Of Lys12valASN95VALCYS117VAL MUTANT OF Human Acidic Fibroblast Growth Factor At 1.70 Angstrom Resolution" 100.00 146 97.73 97.73 9.63e-89 PDB 2J3P "Crystal Structure Of Rat Fgf1 At 1.4 A" 100.00 134 96.97 97.73 5.93e-90 PDB 2K43 "Acidic Fibroblast Growth Factor Solution Structure In The Fgf-1-C2a Binary Complex: Key Component In The Fibroblast Growthfacto" 100.00 133 100.00 100.00 1.37e-92 PDB 2K4A "Fgf-1-C2a Binary Complex Structure: A Key Component In The Fibroblast Growthfactor Non-Classical Pathway" 100.00 133 100.00 100.00 1.37e-92 PDB 2K8R "Solution Structure Of Human Acidic Fibroblast Growth Factor In Complex With Anti-Angiogenic Drug Inositol Hexaphosphate (Ip6)" 100.00 133 100.00 100.00 1.37e-92 PDB 2KI4 "Fgf1-S100a13 Complex Structure: Key Component In Non-Classic Way Of Fgf1" 100.00 133 100.00 100.00 1.37e-92 PDB 2KI6 "The Fgf1-S100a13-C2a Hetero-Hexameric Complex Structure: A C In The Non-Classical Pathway For Fgf1 Secretion" 100.00 133 100.00 100.00 1.37e-92 PDB 2NTD "Human Fibroblast Growth Factor-1 (140 Amino Acid Form) With Cys117valPRO134CYS MUTATIONS" 100.00 146 98.48 98.48 4.24e-89 PDB 2Q9X "Crystal Structure Of Highly Stable Mutant Q40p/s47i/h93g Of Human Fibroblast Growth Factor-1" 100.00 140 97.73 97.73 8.34e-89 PDB 2RQ9 "Solution Structure Of Human Acidic Fibroblast Growth Factor (Afgf) In The Presence Of A Protein Stabilizer Ndsb-New" 100.00 143 100.00 100.00 1.46e-92 PDB 2UUS "Crystal Structure Of The Rat Fgf1-sucrose Octasulfate (sos) Complex" 98.48 132 96.92 97.69 2.25e-88 PDB 3B9U "Crystal Structure Of L26nD28NH93G MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 97.73 98.48 1.15e-88 PDB 3BA4 "Crystal Structure Of L26d Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 8.80e-91 PDB 3BA5 "Crystal Structure Of D28a Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 5.27e-91 PDB 3BA7 "Crystal Structure Of L26nD28A MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 98.48 98.48 7.40e-90 PDB 3BAD "Crystal Structure Of D70aH93G MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 98.48 98.48 2.00e-89 PDB 3BAG "Crystal Structure Of K112nN114A MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 98.48 98.48 2.96e-90 PDB 3BAH "Crystal Structure Of K112n Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 2.30e-91 PDB 3BAO "Crystal Structure Of L26n Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 7.97e-91 PDB 3BAQ "Crystal Structure Of L26a Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 2.76e-91 PDB 3BAU "Crystal Structure Of K12vL26DD28A MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 97.73 97.73 8.17e-89 PDB 3BAV "Crystal Structure Of L26aD28N MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 98.48 99.24 1.25e-90 PDB 3BB2 "Crystal Structure Of L26dD28N MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 98.48 99.24 4.33e-90 PDB 3CQA "Crystal Structure Of Human Fibroblast Growth Factor-1 With Mutations Glu81ala And Lys101ala" 100.00 144 98.48 98.48 9.91e-91 PDB 3CRG "Crystal Structure Of Human Fibroblast Growth Factor-1 With Mutations Glu81ala, Glu82asn And Lys101ala" 100.00 146 97.73 97.73 7.99e-90 PDB 3CRH "Crystal Structure Of Human Fibroblast Growth Factor-1 With Mutations Glu81ser And Lys101ala" 100.00 146 98.48 98.48 1.29e-90 PDB 3CRI "Crystal Structure Of Human Fibroblast Growth Factor-1 With Mutations Glu81ser, Glu82asn And Lys101ala" 100.00 146 97.73 97.73 7.24e-90 PDB 3CU1 "Crystal Structure Of 2:2:2 Fgfr2d2:fgf1:sos Complex" 97.73 131 100.00 100.00 2.24e-90 PDB 3FGM "Crystal Structure Of L44fC83TC117VF132W MUTANT OF HUMAN Acidic Fibroblast Growth Factor" 100.00 146 96.97 97.73 2.02e-88 PDB 3FJ8 "Crystal Structure Of C117i Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 7.63e-91 PDB 3FJ9 "Crystal Structure Of F85w Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 100.00 4.37e-91 PDB 3FJA "Crystal Structure Of F132w Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 100.00 4.37e-91 PDB 3FJB "Crystal Structure Of V31i Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 100.00 1.21e-91 PDB 3FJC "Crystal Structure Of L44w Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 4.52e-91 PDB 3FJD "Crystal Structure Of L44fF132W MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 98.48 99.24 1.36e-90 PDB 3FJE "Crystal Structure Of C83s Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 5.94e-91 PDB 3FJF "Crystal Structure Of C83t Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 6.62e-91 PDB 3FJH "Crystal Structure Of C83a Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 4.28e-91 PDB 3FJI "Crystal Structure Of K12vC83IC117V MUTANT OF HUMAN ACIDIC Fibroblast Growth Factor" 100.00 146 97.73 97.73 6.43e-89 PDB 3FJJ "Crystal Structure Of C83v Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 5.50e-91 PDB 3FJK "Crystal Structure Of A66c Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 4.77e-91 PDB 3HAL "Crystal Structure Of Rabbit Acidic Fibroblast Growth Factor" 100.00 146 97.73 98.48 1.26e-89 PDB 3HOM "Crystal Structure Of Oxidized A66c Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 4.77e-91 PDB 3JUT "Acidic Fibroblast Growth Factor (Fgf-1) Complexed With Gentisic Acid" 98.48 130 100.00 100.00 3.46e-91 PDB 3K1X "Acidic Fibroblast Growth Factor (Fgf-1) Complexed With Dobes" 98.48 130 100.00 100.00 3.46e-91 PDB 3OJ2 "Crystal Structure Of Fgf1 Complexed With The Ectodomain Of Fgfr2b Harboring The A172f Pfeiffer Syndrome Mutation" 100.00 155 100.00 100.00 1.26e-92 PDB 3OJM "Crystal Structure Of Fgf1 Complexed With The Ectodomain Of Fgfr2b Harboring P253r Apert Mutation" 100.00 155 100.00 100.00 1.26e-92 PDB 3OJV "Crystal Structure Of Fgf1 Complexed With The Ectodomain Of Fgfr1c Exhibiting An Ordered Ligand Specificity-Determining Betac'-B" 100.00 136 100.00 100.00 1.09e-92 PDB 3UD7 "Crystal Structure Analysis Of Fgf1-disaccharide(ni21) Complexes" 100.00 141 100.00 100.00 2.67e-92 PDB 3UD8 "Crystal Structure Analysis Of Fgf1-disaccharide(ni22) Complex" 100.00 141 100.00 100.00 2.67e-92 PDB 3UD9 "Crystal Structure Analysis Of Fgf1-disaccharide(ni23) Complex" 100.00 141 100.00 100.00 2.67e-92 PDB 3UDA "Crystal Structure Analysis Of Fgf1-disaccharide(ni24) Complex" 100.00 141 100.00 100.00 2.67e-92 PDB 4J23 "Low Resolution Crystal Structure Of The Fgfr2d2d3/fgf1/sr128545 Complex" 100.00 138 100.00 100.00 1.98e-92 PDB 4Q91 "Crystal Structure Of C16a/k12v/c117v/p134v Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 96.97 96.97 8.00e-88 PDB 4Q9G "Crystal Structure Of K12v/c16s/c117v/p134v Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 96.97 96.97 1.11e-87 PDB 4Q9P "Crystal Structure Of C16t/k12v/c117v/p134v Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 96.97 96.97 1.10e-87 PDB 4QAL "Crystal Structure Of C117a Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 4.28e-91 PDB 4QBC "Crystal Structure Of C117t Mutant Of Human Acidic Fibroblast Growth Factor In Sodium Formate Buffer" 100.00 146 99.24 99.24 6.62e-91 PDB 4QBV "Crystal Structure Of C117t Mutant Of Human Acidic Fibroblast Growth Factor In Sodium Citrate Buffer" 100.00 146 99.24 99.24 7.72e-91 PDB 4QC4 "Crystal Structure Of C117s Mutant Of Human Acidic Fibroblast Growth Factor" 100.00 146 99.24 99.24 5.94e-91 DBJ BAC29035 "unnamed protein product [Mus musculus]" 100.00 155 96.97 97.73 4.07e-90 DBJ BAF82451 "unnamed protein product [Homo sapiens]" 100.00 154 99.24 99.24 5.35e-90 DBJ BAG35227 "unnamed protein product [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 DBJ BAI46827 "fibroblast growth factor 1 [synthetic construct]" 100.00 155 100.00 100.00 1.26e-92 EMBL CAA32448 "unnamed protein product [Rattus norvegicus]" 100.00 155 96.97 97.73 4.07e-90 EMBL CAA36206 "unnamed protein product [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 EMBL CAA42869 "acidic fibroblast growth factor [Sus scrofa]" 97.73 152 97.67 98.45 4.40e-88 EMBL CAA46661 "acidic fibroblast growth factor [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 EMBL CAI29610 "hypothetical protein [Pongo abelii]" 100.00 214 100.00 100.00 1.07e-91 GB AAA37618 "acidic fibroblast growth factor [Mus musculus]" 100.00 155 96.97 97.73 4.07e-90 GB AAA52446 "acidic fibroblast growth factor [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 GB AAA52638 "heparin-binding growth factor 1, partial [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 GB AAA79245 "beta-endothelial cell growth factor [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 GB AAB29057 "acidic fibroblast growth factor, partial [Homo sapiens]" 99.24 154 100.00 100.00 1.06e-91 PRF 1605206A "acidic fibroblast growth factor" 100.00 156 100.00 100.00 1.00e-92 REF NP_000791 "fibroblast growth factor 1 isoform 1 precursor [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 REF NP_001127073 "fibroblast growth factor 1 [Pongo abelii]" 100.00 214 100.00 100.00 1.07e-91 REF NP_001138364 "fibroblast growth factor 1 isoform 1 precursor [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 REF NP_001138406 "fibroblast growth factor 1 isoform 1 precursor [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 REF NP_001138407 "fibroblast growth factor 1 isoform 1 precursor [Homo sapiens]" 100.00 155 100.00 100.00 1.26e-92 SP P05230 "RecName: Full=Fibroblast growth factor 1; Short=FGF-1; AltName: Full=Acidic fibroblast growth factor; Short=aFGF; AltName: Full" 100.00 155 100.00 100.00 1.26e-92 SP P20002 "RecName: Full=Fibroblast growth factor 1; Short=FGF-1; AltName: Full=Acidic fibroblast growth factor; Short=aFGF; AltName: Full" 97.73 152 97.67 98.45 4.40e-88 SP P34004 "RecName: Full=Fibroblast growth factor 1; Short=FGF-1; AltName: Full=Acidic fibroblast growth factor; Short=aFGF; AltName: Full" 100.00 155 97.73 97.73 1.82e-90 SP P61148 "RecName: Full=Fibroblast growth factor 1; Short=FGF-1; AltName: Full=Acidic fibroblast growth factor; Short=aFGF; AltName: Full" 100.00 155 96.97 97.73 4.07e-90 SP P61149 "RecName: Full=Fibroblast growth factor 1; Short=FGF-1; AltName: Full=Acidic fibroblast growth factor; Short=aFGF; AltName: Full" 100.00 155 96.97 97.73 4.07e-90 stop_ save_ ############# # Ligands # ############# save_NTS _Saveframe_category ligand _Mol_type non-polymer _Name_common "NTS (NAPHTHALENE TRISULFONATE)" _BMRB_code . _PDB_code NTS _Molecular_mass 365.336 _Mol_charge -3 _Mol_paramagnetic no _Mol_aromatic yes _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Wed Feb 16 17:27:39 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons C1 C1 C . 0 . ? C2 C2 C . 0 . ? C3 C3 C . 0 . ? C4 C4 C . 0 . ? C5 C5 C . 0 . ? C6 C6 C . 0 . ? C7 C7 C . 0 . ? C8 C8 C . 0 . ? C9 C9 C . 0 . ? C10 C10 C . 0 . ? O11 O11 O . 0 . ? O12 O12 O . 0 . ? O13 O13 O . -1 . ? O31 O31 O . 0 . ? O32 O32 O . 0 . ? O33 O33 O . -1 . ? O61 O61 O . 0 . ? O62 O62 O . 0 . ? O63 O63 O . -1 . ? S1 S1 S . 0 . ? S3 S3 S . 0 . ? S6 S6 S . 0 . ? H2 H2 H . 0 . ? H4 H4 H . 0 . ? H5 H5 H . 0 . ? H7 H7 H . 0 . ? H8 H8 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name DOUB C1 C2 ? ? SING C1 C9 ? ? SING C1 S1 ? ? SING C2 C3 ? ? SING C2 H2 ? ? DOUB C3 C4 ? ? SING C3 S3 ? ? SING C4 C10 ? ? SING C4 H4 ? ? DOUB C5 C6 ? ? SING C5 C10 ? ? SING C5 H5 ? ? SING C6 C7 ? ? SING C6 S6 ? ? DOUB C7 C8 ? ? SING C7 H7 ? ? SING C8 C9 ? ? SING C8 H8 ? ? DOUB C9 C10 ? ? DOUB O11 S1 ? ? DOUB O12 S1 ? ? SING O13 S1 ? ? DOUB O31 S3 ? ? DOUB O32 S3 ? ? SING O33 S3 ? ? DOUB O61 S6 ? ? DOUB O62 S6 ? ? SING O63 S6 ? ? stop_ _Mol_thiol_state 'not present' _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $FGF human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $FGF 'recombinant technology' . Escherichia coli . pMG47 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $FGF 22 mg/ml 'natural abundance' 'beta mercaptoethanol' 1 mM 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' $NTS 'bound to FGF' mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_LSQKAB _Saveframe_category software _Name LSQKAB _Version . loop_ _Vendor _Address _Electronic_address '(Collaborative Computational Project, Number 4, 1994).' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AMX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_TOCSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.0 . pH pressure 1 . atm temperature 298 . K stop_ save_