data_17465 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural Characterisation of Apoflavodoxin shows that the Location of the Stable Nucleus Differs Among Proteins with a Flavodoxin-like Topology ; _BMRB_accession_number 17465 _BMRB_flat_file_name bmr17465.str _Entry_type original _Submission_date 2011-02-11 _Accession_date 2011-02-11 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Steensma Elles . . 2 'van Mierlo' Carlo P.M. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count chemical_rates 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-06-06 original author . stop_ _Original_release_date 2012-06-06 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural Characterisation of Apoflavodoxin shows that the Location of the Stable Nucleus Differs Among Proteins with a Flavodoxin-like Topology' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Steensma Elles . . 2 'van Mierlo' Carlo P.M. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 282 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 653 _Page_last 666 _Year 1998 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'aII exchange' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label aII $aII stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_aII _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common aII _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 179 _Mol_residue_sequence ; AKIGLFFGSNTGKTRKVAKS IKKRFDDETMSDALNVNRVS AEDFAQYQFLILGTPTLGEG ELPGLSSDAENESWEEFLPK IEGLDFSGKTVALFGLGDQV GYPENYLDALGELYSFFKDR GAKIVGSWSTDGYEFESSEA VVDGKFVGLALDLDNQSGKT DERVAAWLAQIAPEFGLSL ; loop_ _Residue_seq_code _Residue_label 1 ALA 2 LYS 3 ILE 4 GLY 5 LEU 6 PHE 7 PHE 8 GLY 9 SER 10 ASN 11 THR 12 GLY 13 LYS 14 THR 15 ARG 16 LYS 17 VAL 18 ALA 19 LYS 20 SER 21 ILE 22 LYS 23 LYS 24 ARG 25 PHE 26 ASP 27 ASP 28 GLU 29 THR 30 MET 31 SER 32 ASP 33 ALA 34 LEU 35 ASN 36 VAL 37 ASN 38 ARG 39 VAL 40 SER 41 ALA 42 GLU 43 ASP 44 PHE 45 ALA 46 GLN 47 TYR 48 GLN 49 PHE 50 LEU 51 ILE 52 LEU 53 GLY 54 THR 55 PRO 56 THR 57 LEU 58 GLY 59 GLU 60 GLY 61 GLU 62 LEU 63 PRO 64 GLY 65 LEU 66 SER 67 SER 68 ASP 69 ALA 70 GLU 71 ASN 72 GLU 73 SER 74 TRP 75 GLU 76 GLU 77 PHE 78 LEU 79 PRO 80 LYS 81 ILE 82 GLU 83 GLY 84 LEU 85 ASP 86 PHE 87 SER 88 GLY 89 LYS 90 THR 91 VAL 92 ALA 93 LEU 94 PHE 95 GLY 96 LEU 97 GLY 98 ASP 99 GLN 100 VAL 101 GLY 102 TYR 103 PRO 104 GLU 105 ASN 106 TYR 107 LEU 108 ASP 109 ALA 110 LEU 111 GLY 112 GLU 113 LEU 114 TYR 115 SER 116 PHE 117 PHE 118 LYS 119 ASP 120 ARG 121 GLY 122 ALA 123 LYS 124 ILE 125 VAL 126 GLY 127 SER 128 TRP 129 SER 130 THR 131 ASP 132 GLY 133 TYR 134 GLU 135 PHE 136 GLU 137 SER 138 SER 139 GLU 140 ALA 141 VAL 142 VAL 143 ASP 144 GLY 145 LYS 146 PHE 147 VAL 148 GLY 149 LEU 150 ALA 151 LEU 152 ASP 153 LEU 154 ASP 155 ASN 156 GLN 157 SER 158 GLY 159 LYS 160 THR 161 ASP 162 GLU 163 ARG 164 VAL 165 ALA 166 ALA 167 TRP 168 LEU 169 ALA 170 GLN 171 ILE 172 ALA 173 PRO 174 GLU 175 PHE 176 GLY 177 LEU 178 SER 179 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15474 Apoflavodoxin 100.00 179 100.00 100.00 7.46e-123 PDB 1YOB "C69a Flavodoxin Ii From Azotobacter Vinelandii" 100.00 179 100.00 100.00 7.46e-123 GB AAA22154 "flavodoxin [Azotobacter vinelandii]" 100.00 180 99.44 99.44 1.93e-122 GB AAA64735 "flavodoxin (nifF) [Azotobacter vinelandii]" 100.00 180 99.44 99.44 1.93e-122 GB ACO76434 "Flavodoxin, nifF [Azotobacter vinelandii DJ]" 100.00 180 99.44 99.44 1.93e-122 GB AGK13779 "Flavodoxin, nifF [Azotobacter vinelandii CA]" 100.00 180 99.44 99.44 1.93e-122 GB AGK18380 "Flavodoxin, nifF [Azotobacter vinelandii CA6]" 100.00 180 99.44 99.44 1.93e-122 PRF 752055A flavodoxin 100.00 179 98.88 99.44 1.11e-121 REF WP_012698862 "flavodoxin-2 [Azotobacter vinelandii]" 100.00 180 99.44 99.44 1.93e-122 SP P00324 "RecName: Full=Flavodoxin-2" 100.00 180 99.44 99.44 1.93e-122 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $aII 'A. vinelandii' 354 Bacteria . Azotobacter vinelandii 'ATCC 478' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $aII 'recombinant technology' . Escherichia coli . ns stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $aII 2 mM [U-15N] 'potassium pyrophosphate' 150 mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_Felix _Saveframe_category software _Name FELIX _Version 2.5 loop_ _Vendor _Address _Electronic_address 'Accelrys Software Inc.' . . stop_ loop_ _Task processing stop_ _Details . save_ save_XEASY _Saveframe_category software _Name XEASY _Version . loop_ _Vendor _Address _Electronic_address 'Bartels et al.' . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AMX _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH* 6.2 . pH pressure 1 . atm temperature 303 0.5 K stop_ save_