data_17631 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for human JAZ ZF2 ; _BMRB_accession_number 17631 _BMRB_flat_file_name bmr17631.str _Entry_type original _Submission_date 2011-05-08 _Accession_date 2011-05-08 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Burge Russell G. . 2 Martinez-Yamout Maria A. . 3 Gottesfeld Joel M. . 4 Dyson H. J. . 5 Wright Peter E. . 6 Dyson Jane H. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 45 "13C chemical shifts" 80 "15N chemical shifts" 45 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-02-24 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 17630 'Zinc Finger Protein residues 23-117' stop_ _Original_release_date 2014-02-24 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Backbone assignment of human JAZ ZF2' _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Burge Russell G. . 2 Martinez-Yamout Maria A. . 3 Gottesfeld Joel M. . 4 Dyson H. J. . 5 Wright Peter E. . stop_ _Journal_abbreviation Biochemistry _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'JAZ ZF2' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'JAZ ZF2' $JAZ_ZF2 'zinc ion' $ZN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_JAZ_ZF2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common JAZ_ZF2 _Molecular_mass . _Mol_thiol_state 'free and other bound' loop_ _Biological_function 'dsRNA binding' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 55 _Mol_residue_sequence ; GETKKLDSDQKSSRSKDKNQ CCPICNMTFSSPVVAQSHYL GKTHAKNLKLKQQST ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 115 GLY 2 116 GLU 3 117 THR 4 118 LYS 5 119 LYS 6 120 LEU 7 121 ASP 8 122 SER 9 123 ASP 10 124 GLN 11 125 LYS 12 126 SER 13 127 SER 14 128 ARG 15 129 SER 16 130 LYS 17 131 ASP 18 132 LYS 19 133 ASN 20 134 GLN 21 135 CYS 22 136 CYS 23 137 PRO 24 138 ILE 25 139 CYS 26 140 ASN 27 141 MET 28 142 THR 29 143 PHE 30 144 SER 31 145 SER 32 146 PRO 33 147 VAL 34 148 VAL 35 149 ALA 36 150 GLN 37 151 SER 38 152 HIS 39 153 TYR 40 154 LEU 41 155 GLY 42 156 LYS 43 157 THR 44 158 HIS 45 159 ALA 46 160 LYS 47 161 ASN 48 162 LEU 49 163 LYS 50 164 LEU 51 165 LYS 52 166 GLN 53 167 GLN 54 168 SER 55 169 THR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAD18614 "unnamed protein product [Homo sapiens]" 100.00 319 100.00 100.00 1.10e-28 DBJ BAG72878 "zinc finger protein 346 [synthetic construct]" 100.00 294 98.18 100.00 5.32e-28 EMBL CAH18475 "hypothetical protein [Homo sapiens]" 100.00 294 98.18 100.00 5.32e-28 EMBL CAL37677 "hypothetical protein [synthetic construct]" 100.00 294 98.18 100.00 5.32e-28 EMBL CAL38643 "hypothetical protein, partial [synthetic construct]" 100.00 294 98.18 100.00 5.32e-28 GB AAD52018 "double-stranded RNA-binding zinc finger protein JAZ [Homo sapiens]" 100.00 294 100.00 100.00 8.31e-29 GB AAH07775 "Zinc finger protein 346 [Homo sapiens]" 100.00 294 100.00 100.00 8.31e-29 GB AIC50988 "ZNF346, partial [synthetic construct]" 100.00 294 100.00 100.00 8.31e-29 GB EAW85038 "zinc finger protein 346, isoform CRA_a [Homo sapiens]" 100.00 232 100.00 100.00 2.10e-29 GB EAW85040 "zinc finger protein 346, isoform CRA_c [Homo sapiens]" 100.00 294 100.00 100.00 8.31e-29 REF NP_001231409 "zinc finger protein 346 [Sus scrofa]" 100.00 294 100.00 100.00 6.89e-29 REF NP_001295142 "zinc finger protein 346 isoform b [Homo sapiens]" 100.00 319 100.00 100.00 1.10e-28 REF NP_001295144 "zinc finger protein 346 isoform d [Homo sapiens]" 100.00 232 100.00 100.00 2.10e-29 REF NP_036411 "zinc finger protein 346 isoform a [Homo sapiens]" 100.00 294 100.00 100.00 8.31e-29 REF XP_001498579 "PREDICTED: zinc finger protein 346 isoform X1 [Equus caballus]" 100.00 233 100.00 100.00 4.69e-29 SP Q9UL40 "RecName: Full=Zinc finger protein 346; AltName: Full=Just another zinc finger protein" 100.00 294 100.00 100.00 8.31e-29 stop_ save_ ############# # Ligands # ############# save_ZN _Saveframe_category ligand _Mol_type non-polymer _Name_common "ZN (ZINC ION)" _BMRB_code . _PDB_code ZN _Molecular_mass 65.409 _Mol_charge 2 _Mol_paramagnetic no _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Tue May 10 10:02:43 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons ZN ZN ZN . 2 . ? stop_ _Mol_thiol_state 'not present' _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $JAZ_ZF2 human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $JAZ_ZF2 'recombinant technology' . Escherichia coli . pET21a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $JAZ_ZF2 300 uM '[U-98% 13C; U-98% 15N]' TRIS 25 mM 'natural abundance' 'potassium chloride' 50 mM 'natural abundance' D2O 7 % '[U-99% 2H]' DTT 2 mM 'natural abundance' $ZN 50 uM 'natural abundance' H2O 90 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRView _Saveframe_category software _Name NMRView _Version 5 loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 75 . mM pH 7.3 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0 external indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0 external direct . . . 1.0 DSS N 15 'methyl protons' ppm 0 external indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCA' '3D HNCACB' '3D HNCO' '3D HN(CO)CA' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'JAZ ZF2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 116 2 GLU CA C 56.1 0.1 1 2 117 3 THR H H 8.316 0.01 1 3 117 3 THR CA C 62 0.1 1 4 117 3 THR N N 117.017 0.2 1 5 118 4 LYS H H 7.8 0.01 1 6 118 4 LYS CA C 56 0.1 1 7 118 4 LYS N N 116.882 0.2 1 8 119 5 LYS H H 7.304 0.01 1 9 119 5 LYS CA C 58.3 0.1 1 10 119 5 LYS N N 118.114 0.2 1 11 120 6 LEU H H 7.585 0.01 1 12 120 6 LEU N N 118.665 0.2 1 13 121 7 ASP H H 8.25 0.01 1 14 121 7 ASP CA C 55.37 0.1 1 15 121 7 ASP N N 121.5 0.2 1 16 122 8 SER H H 8.079 0.01 1 17 122 8 SER CA C 58.27 0.1 1 18 122 8 SER N N 115.272 0.2 1 19 123 9 ASP H H 8.232 0.01 1 20 123 9 ASP CA C 59 0.1 1 21 123 9 ASP N N 122.002 0.2 1 22 124 10 GLN CA C 55.3 0.1 1 23 125 11 LYS CA C 54.3 0.1 1 24 128 14 ARG H H 8.109 0.01 1 25 128 14 ARG CA C 56.2 0.1 1 26 128 14 ARG N N 122.565 0.2 1 27 129 15 SER H H 7.875 0.01 1 28 129 15 SER C C 174.69 0.1 1 29 129 15 SER CA C 58.62 0.1 1 30 129 15 SER N N 116.198 0.2 1 31 130 16 LYS H H 8.109 0.01 1 32 130 16 LYS CA C 56.2 0.1 1 33 130 16 LYS N N 122.565 0.2 1 34 131 17 ASP H H 8.089 0.01 1 35 131 17 ASP C C 177.154 0.1 1 36 131 17 ASP CA C 53.928 0.1 1 37 131 17 ASP N N 121.483 0.2 1 38 132 18 LYS H H 8.482 0.01 1 39 132 18 LYS C C 177.267 0.1 1 40 132 18 LYS CA C 57.708 0.1 1 41 132 18 LYS N N 124.354 0.2 1 42 133 19 ASN H H 8.464 0.01 1 43 133 19 ASN C C 175.031 0.1 1 44 133 19 ASN CA C 53.78 0.1 1 45 133 19 ASN N N 116.624 0.2 1 46 134 20 GLN H H 8.05 0.01 1 47 134 20 GLN C C 175.705 0.1 1 48 134 20 GLN CA C 56.6 0.1 1 49 134 20 GLN N N 117.299 0.2 1 50 135 21 CYS H H 8.016 0.01 1 51 135 21 CYS C C 175.705 0.1 1 52 135 21 CYS CA C 56.557 0.1 1 53 135 21 CYS N N 117.809 0.2 1 54 136 22 CYS H H 8.291 0.01 1 55 136 22 CYS C C 172.04 0.1 1 56 136 22 CYS CA C 55.407 0.1 1 57 136 22 CYS N N 127.837 0.2 1 58 137 23 PRO C C 63.701 0.1 1 59 138 24 ILE C C 177.46 0.1 1 60 138 24 ILE CA C 63.087 0.1 1 61 139 25 CYS H H 8.635 0.01 1 62 139 25 CYS C C 175.12 0.1 1 63 139 25 CYS CA C 60.2 0.1 1 64 139 25 CYS N N 121.55 0.2 1 65 140 26 ASN H H 7.931 0.01 1 66 140 26 ASN C C 173.49 0.1 1 67 140 26 ASN CA C 53.75 0.1 1 68 140 26 ASN N N 117.079 0.2 1 69 141 27 MET H H 6.993 0.01 1 70 141 27 MET C C 173.53 0.1 1 71 141 27 MET CA C 54.08 0.1 1 72 141 27 MET N N 115.263 0.2 1 73 142 28 THR H H 7.852 0.01 1 74 142 28 THR C C 172.77 0.1 1 75 142 28 THR CA C 59.905 0.1 1 76 142 28 THR N N 114.426 0.2 1 77 143 29 PHE H H 7.703 0.01 1 78 143 29 PHE C C 172.77 0.1 1 79 143 29 PHE CA C 59.92 0.1 1 80 143 29 PHE N N 119.726 0.2 1 81 144 30 SER H H 8.138 0.01 1 82 144 30 SER C C 173.752 0.1 1 83 144 30 SER CA C 58.924 0.1 1 84 144 30 SER N N 122.297 0.2 1 85 145 31 SER H H 7.251 0.01 1 86 145 31 SER CA C 55.52 0.1 1 87 145 31 SER N N 114.651 0.2 1 88 146 32 PRO CA C 64.401 0.1 1 89 147 33 VAL H H 7.456 0.01 1 90 147 33 VAL CA C 65.203 0.1 1 91 147 33 VAL N N 117.972 0.2 1 92 148 34 VAL H H 6.989 0.01 1 93 148 34 VAL C C 178.662 0.1 1 94 148 34 VAL CA C 65.303 0.1 1 95 148 34 VAL N N 121.68 0.2 1 96 149 35 ALA H H 7.386 0.01 1 97 149 35 ALA C C 177.842 0.1 1 98 149 35 ALA CA C 54.508 0.1 1 99 149 35 ALA N N 123.545 0.2 1 100 150 36 GLN H H 7.913 0.01 1 101 150 36 GLN C C 178.662 0.1 1 102 150 36 GLN CA C 59.106 0.1 1 103 150 36 GLN N N 115.515 0.2 1 104 151 37 SER H H 7.406 0.01 1 105 151 37 SER C C 177.172 0.1 1 106 151 37 SER CA C 61.301 0.1 1 107 151 37 SER N N 112.625 0.2 1 108 152 38 HIS H H 7.682 0.01 1 109 152 38 HIS C C 178.603 0.1 1 110 152 38 HIS CA C 60.108 0.1 1 111 152 38 HIS N N 121.559 0.2 1 112 153 39 TYR H H 7.64 0.01 1 113 153 39 TYR C C 176.723 0.1 1 114 153 39 TYR CA C 61.5 0.1 1 115 153 39 TYR N N 116.921 0.2 1 116 154 40 LEU H H 7.157 0.01 1 117 154 40 LEU C C 177.732 0.1 1 118 154 40 LEU CA C 61.5 0.1 1 119 154 40 LEU N N 118.895 0.2 1 120 155 41 GLY H H 7.358 0.01 1 121 155 41 GLY C C 175.383 0.1 1 122 155 41 GLY CA C 44.963 0.1 1 123 155 41 GLY N N 106.691 0.2 1 124 156 42 LYS H H 8.364 0.01 1 125 156 42 LYS CA C 57 0.1 1 126 156 42 LYS N N 117.914 0.2 1 127 157 43 THR C C 175.25 0.1 1 128 157 43 THR CA C 67.005 0.1 1 129 158 44 HIS H H 8.579 0.01 1 130 158 44 HIS C C 175.253 0.1 1 131 158 44 HIS CA C 60.2 0.1 1 132 158 44 HIS N N 121.201 0.2 1 133 159 45 ALA H H 7.008 0.01 1 134 159 45 ALA CA C 55.108 0.1 1 135 159 45 ALA N N 117.893 0.2 1 136 160 46 LYS H H 7.842 0.01 1 137 160 46 LYS CA C 58.924 0.1 1 138 160 46 LYS N N 118.471 0.2 1 139 161 47 ASN H H 7.842 0.01 1 140 161 47 ASN CA C 58.924 0.1 1 141 161 47 ASN N N 118.433 0.2 1 142 162 48 LEU H H 8.192 0.01 1 143 162 48 LEU C C 176.43 0.1 1 144 162 48 LEU CA C 54.4 0.1 1 145 162 48 LEU N N 122.275 0.2 1 146 163 49 LYS H H 8.153 0.01 1 147 163 49 LYS CA C 56.218 0.1 1 148 163 49 LYS N N 120.333 0.2 1 149 164 50 LEU H H 8.2 0.01 1 150 164 50 LEU CA C 54.4 0.1 1 151 164 50 LEU N N 121.3 0.2 1 152 165 51 LYS H H 7.714 0.01 1 153 165 51 LYS C C 177.41 0.1 1 154 165 51 LYS CA C 56.922 0.1 1 155 165 51 LYS N N 119.092 0.2 1 156 166 52 GLN H H 7.885 0.01 1 157 166 52 GLN C C 176.21 0.1 1 158 166 52 GLN CA C 56.511 0.1 1 159 166 52 GLN N N 119.243 0.2 1 160 167 53 GLN H H 7.995 0.01 1 161 167 53 GLN C C 175.742 0.1 1 162 167 53 GLN CA C 55.926 0.1 1 163 167 53 GLN N N 119.671 0.2 1 164 168 54 SER H H 8.083 0.01 1 165 168 54 SER C C 173.69 0.1 1 166 168 54 SER CA C 58.293 0.1 1 167 168 54 SER N N 116.927 0.2 1 168 169 55 THR H H 7.677 0.01 1 169 169 55 THR CA C 63.3 0.1 1 170 169 55 THR N N 120.746 0.2 1 stop_ save_