data_18185 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H and 15N Chemical Shift Assignments of the VHS-UIM domains of STAM2 ; _BMRB_accession_number 18185 _BMRB_flat_file_name bmr18185.str _Entry_type original _Submission_date 2012-01-06 _Accession_date 2012-01-06 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Walker Olivier . . 2 Lange Anja . . 3 Castaneda Carlos . . 4 Hoeller Daniela . . 5 Lancelin Jean-Marc . . 6 Fushman David . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 165 "15N chemical shifts" 165 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2013-03-25 update BMRB 'update entry citation' 2012-04-05 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Evidence for cooperative and domain-specific binding of the signal transducing adaptor molecule 2 (STAM2) to Lys63-linked diubiquitin.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 22493438 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lange Anja . . 2 Castaneda Carlos . . 3 Hoeller Daniela . . 4 Lancelin Jean-Marc . . 5 Fushman David . . 6 Walker Olivier . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 287 _Journal_issue 22 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 18687 _Page_last 18699 _Year 2012 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'VHS-UIM monomer' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label VHS-UIM $VHS-UIM_domains_of_STAM2 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_VHS-UIM_domains_of_STAM2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common VHS-UIM_domains_of_STAM2 _Molecular_mass . _Mol_thiol_state unknown _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 192 _Mol_residue_sequence ; GAMGMPLFTANPFEQDVEKA TNEYNTTEDWSLIMDICDKV GSTPNGAKDCLKAIMKRVNH KVPHVALQALTLLGACVANC GKIFRLGVCSRDFATEVRAV IKNKAHPKVCEKLKSLMVEW SEEFQKDPQFSLISATIKSM KEEGITFPPAGSQTVSAAAK NGTSSNKNKEDEDIAKAIEL SLQEQKQQHTET ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 4 GLY 2 5 ALA 3 6 MET 4 7 GLY 5 8 MET 6 9 PRO 7 10 LEU 8 11 PHE 9 12 THR 10 13 ALA 11 14 ASN 12 15 PRO 13 16 PHE 14 17 GLU 15 18 GLN 16 19 ASP 17 20 VAL 18 21 GLU 19 22 LYS 20 23 ALA 21 24 THR 22 25 ASN 23 26 GLU 24 27 TYR 25 28 ASN 26 29 THR 27 30 THR 28 31 GLU 29 32 ASP 30 33 TRP 31 34 SER 32 35 LEU 33 36 ILE 34 37 MET 35 38 ASP 36 39 ILE 37 40 CYS 38 41 ASP 39 42 LYS 40 43 VAL 41 44 GLY 42 45 SER 43 46 THR 44 47 PRO 45 48 ASN 46 49 GLY 47 50 ALA 48 51 LYS 49 52 ASP 50 53 CYS 51 54 LEU 52 55 LYS 53 56 ALA 54 57 ILE 55 58 MET 56 59 LYS 57 60 ARG 58 61 VAL 59 62 ASN 60 63 HIS 61 64 LYS 62 65 VAL 63 66 PRO 64 67 HIS 65 68 VAL 66 69 ALA 67 70 LEU 68 71 GLN 69 72 ALA 70 73 LEU 71 74 THR 72 75 LEU 73 76 LEU 74 77 GLY 75 78 ALA 76 79 CYS 77 80 VAL 78 81 ALA 79 82 ASN 80 83 CYS 81 84 GLY 82 85 LYS 83 86 ILE 84 87 PHE 85 88 ARG 86 89 LEU 87 90 GLY 88 91 VAL 89 92 CYS 90 93 SER 91 94 ARG 92 95 ASP 93 96 PHE 94 97 ALA 95 98 THR 96 99 GLU 97 100 VAL 98 101 ARG 99 102 ALA 100 103 VAL 101 104 ILE 102 105 LYS 103 106 ASN 104 107 LYS 105 108 ALA 106 109 HIS 107 110 PRO 108 111 LYS 109 112 VAL 110 113 CYS 111 114 GLU 112 115 LYS 113 116 LEU 114 117 LYS 115 118 SER 116 119 LEU 117 120 MET 118 121 VAL 119 122 GLU 120 123 TRP 121 124 SER 122 125 GLU 123 126 GLU 124 127 PHE 125 128 GLN 126 129 LYS 127 130 ASP 128 131 PRO 129 132 GLN 130 133 PHE 131 134 SER 132 135 LEU 133 136 ILE 134 137 SER 135 138 ALA 136 139 THR 137 140 ILE 138 141 LYS 139 142 SER 140 143 MET 141 144 LYS 142 145 GLU 143 146 GLU 144 147 GLY 145 148 ILE 146 149 THR 147 150 PHE 148 151 PRO 149 152 PRO 150 153 ALA 151 154 GLY 152 155 SER 153 156 GLN 154 157 THR 155 158 VAL 156 159 SER 157 160 ALA 158 161 ALA 159 162 ALA 160 163 LYS 161 164 ASN 162 165 GLY 163 166 THR 164 167 SER 165 168 SER 166 169 ASN 167 170 LYS 168 171 ASN 169 172 LYS 170 173 GLU 171 174 ASP 172 175 GLU 173 176 ASP 174 177 ILE 175 178 ALA 176 179 LYS 177 180 ALA 178 181 ILE 179 182 GLU 180 183 LEU 181 184 SER 182 185 LEU 183 186 GLN 184 187 GLU 185 188 GLN 186 189 LYS 187 190 GLN 188 191 GLN 189 192 HIS 190 193 THR 191 194 GLU 192 195 THR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-14 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 10264 "VHS domain" 80.21 163 97.40 98.05 5.43e-107 PDB 1X5B "The Solution Structure Of The Vhs Domain Of Human Signal Transducing Adaptor Molecule 2" 80.21 163 97.40 98.05 5.43e-107 PDB 2L0T "Solution Structure Of The Complex Of Ubiquitin And The Vhs Domain Of Stam2" 80.21 163 97.40 98.05 5.43e-107 DBJ BAF84954 "unnamed protein product [Homo sapiens]" 97.92 525 98.94 98.94 1.45e-130 DBJ BAF85536 "unnamed protein product [Homo sapiens]" 97.92 525 98.94 98.94 1.45e-130 DBJ BAG72771 "signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 [synthetic construct]" 97.92 525 100.00 100.00 9.95e-133 DBJ BAK62690 "signal transducing adapter molecule 2 [Pan troglodytes]" 97.92 525 98.40 98.94 1.17e-129 EMBL CAB63735 "hypothetical protein [Homo sapiens]" 97.92 525 100.00 100.00 9.95e-133 EMBL CAL38297 "hypothetical protein [synthetic construct]" 97.92 525 100.00 100.00 9.95e-133 GB AAC63963 "signal transducing adaptor molecule 2A [Homo sapiens]" 97.92 525 98.94 98.94 1.45e-130 GB AAC63964 "signal transducing adaptor molecule 2B [Homo sapiens]" 97.92 342 98.94 98.94 1.52e-131 GB AAH28740 "Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 [Homo sapiens]" 97.92 525 98.94 98.94 1.45e-130 GB AAI12605 "Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 [Bos taurus]" 97.92 525 98.40 98.94 8.39e-130 GB AAY14712 "unknown [Homo sapiens]" 97.92 525 98.94 98.94 1.45e-130 REF NP_001069574 "signal transducing adapter molecule 2 [Bos taurus]" 97.92 525 98.40 98.94 8.39e-130 REF NP_001267210 "signal transducing adapter molecule 2 [Pan troglodytes]" 97.92 525 98.40 98.94 1.17e-129 REF NP_005834 "signal transducing adapter molecule 2 [Homo sapiens]" 97.92 525 98.94 98.94 1.45e-130 REF XP_002749486 "PREDICTED: signal transducing adapter molecule 2 [Callithrix jacchus]" 97.92 525 97.87 97.87 1.30e-128 REF XP_002926244 "PREDICTED: signal transducing adapter molecule 2 isoform X1 [Ailuropoda melanoleuca]" 97.92 525 97.87 98.40 5.97e-129 SP O75886 "RecName: Full=Signal transducing adapter molecule 2; Short=STAM-2; AltName: Full=Hrs-binding protein" 97.92 525 98.94 98.94 1.45e-130 TPG DAA32691 "TPA: signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 [Bos taurus]" 97.92 436 98.40 98.94 4.42e-130 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $VHS-UIM_domains_of_STAM2 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $VHS-UIM_domains_of_STAM2 'recombinant technology' . Escherichia coli . pETEM-60 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $VHS-UIM_domains_of_STAM2 200-400 uM '[U-13C; U-15N]' 'sodium phosphate' 20 mM 'natural abundance' 'sodium azide' 0.02 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_VNMRJ _Saveframe_category software _Name VNMRJ _Version . loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task collection stop_ _Details . save_ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_HNCO_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_1H-13C_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_1 save_ save_NMR_spectrometer_expt _Saveframe_category NMR_applied_experiment _Experiment_name . _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.8 . pH pressure 1 . atm temperature 288 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.7 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.0 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNCO' '3D HN(CO)CA' '2D 1H-15N HSQC' '3D 1H-13C NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name VHS-UIM _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 6 3 MET H H 8.3950 . 1 2 6 3 MET N N 121.0020 . 1 3 7 4 GLY H H 8.2970 . 1 4 7 4 GLY N N 111.2400 . 1 5 8 5 MET H H 7.9810 . 1 6 8 5 MET N N 122.0230 . 1 7 10 7 LEU H H 8.1440 . 1 8 10 7 LEU N N 122.9370 . 1 9 11 8 PHE H H 8.0370 . 1 10 11 8 PHE N N 121.7070 . 1 11 12 9 THR H H 7.9010 . 1 12 12 9 THR N N 117.9730 . 1 13 13 10 ALA H H 8.1130 . 1 14 13 10 ALA N N 127.7710 . 1 15 14 11 ASN H H 8.4770 . 1 16 14 11 ASN N N 121.5910 . 1 17 16 13 PHE H H 8.6490 . 1 18 16 13 PHE N N 116.7050 . 1 19 17 14 GLU H H 7.4250 . 1 20 17 14 GLU N N 121.3090 . 1 21 18 15 GLN H H 8.3380 . 1 22 18 15 GLN N N 119.1100 . 1 23 19 16 ASP H H 8.4090 . 1 24 19 16 ASP N N 122.0870 . 1 25 20 17 VAL H H 8.3090 . 1 26 20 17 VAL N N 119.4980 . 1 27 21 18 GLU H H 8.3320 . 1 28 21 18 GLU N N 125.0080 . 1 29 22 19 LYS H H 7.6080 . 1 30 22 19 LYS N N 119.9760 . 1 31 23 20 ALA H H 8.0130 . 1 32 23 20 ALA N N 120.7100 . 1 33 24 21 THR H H 7.0660 . 1 34 24 21 THR N N 105.1180 . 1 35 25 22 ASN H H 6.3280 . 1 36 25 22 ASN N N 123.1610 . 1 37 26 23 GLU H H 9.1760 . 1 38 26 23 GLU N N 128.0450 . 1 39 27 24 TYR H H 8.1230 . 1 40 27 24 TYR N N 117.0870 . 1 41 28 25 ASN H H 7.3640 . 1 42 28 25 ASN N N 120.6500 . 1 43 29 26 THR H H 9.0200 . 1 44 29 26 THR N N 115.5070 . 1 45 30 27 THR H H 7.4390 . 1 46 30 27 THR N N 115.4810 . 1 47 31 28 GLU H H 8.5110 . 1 48 31 28 GLU N N 124.2850 . 1 49 32 29 ASP H H 8.6800 . 1 50 32 29 ASP N N 124.8250 . 1 51 33 30 TRP H H 8.4800 . 1 52 33 30 TRP N N 125.5280 . 1 53 34 31 SER H H 8.2180 . 1 54 34 31 SER N N 118.0360 . 1 55 35 32 LEU H H 7.0550 . 1 56 35 32 LEU N N 124.7610 . 1 57 36 33 ILE H H 7.6640 . 1 58 36 33 ILE N N 119.4640 . 1 59 38 35 ASP H H 7.7740 . 1 60 38 35 ASP N N 123.0650 . 1 61 40 37 CYS H H 7.8390 . 1 62 40 37 CYS N N 117.3390 . 1 63 43 40 VAL H H 8.2120 . 1 64 43 40 VAL N N 122.9780 . 1 65 44 41 GLY H H 7.2950 . 1 66 44 41 GLY N N 103.0640 . 1 67 45 42 SER H H 7.5860 . 1 68 45 42 SER N N 113.5970 . 1 69 46 43 THR H H 7.2410 . 1 70 46 43 THR N N 118.5520 . 1 71 49 46 GLY H H 8.1390 . 1 72 49 46 GLY N N 107.9410 . 1 73 50 47 ALA H H 8.3370 . 1 74 50 47 ALA N N 122.6970 . 1 75 51 48 LYS H H 7.5020 . 1 76 51 48 LYS N N 119.2000 . 1 77 53 50 CYS H H 8.3130 . 1 78 53 50 CYS N N 123.1480 . 1 79 54 51 LEU H H 7.6640 . 1 80 54 51 LEU N N 119.4640 . 1 81 55 52 LYS H H 7.5680 . 1 82 55 52 LYS N N 118.0100 . 1 83 56 53 ALA H H 7.5550 . 1 84 56 53 ALA N N 121.5900 . 1 85 58 55 MET H H 8.5890 . 1 86 58 55 MET N N 117.9240 . 1 87 59 56 LYS H H 7.3420 . 1 88 59 56 LYS N N 119.9760 . 1 89 60 57 ARG H H 7.1220 . 1 90 60 57 ARG N N 117.5750 . 1 91 62 59 ASN H H 6.7800 . 1 92 62 59 ASN N N 113.2540 . 1 93 63 60 HIS H H 7.2700 . 1 94 63 60 HIS N N 123.7810 . 1 95 64 61 LYS H H 7.6270 . 1 96 64 61 LYS N N 123.8910 . 1 97 65 62 VAL H H 11.3000 . 1 98 65 62 VAL N N 130.4230 . 1 99 67 64 HIS H H 8.0290 . 1 100 67 64 HIS N N 114.1410 . 1 101 68 65 VAL H H 7.1040 . 1 102 68 65 VAL N N 120.2900 . 1 103 69 66 ALA H H 6.7980 . 1 104 69 66 ALA N N 123.2320 . 1 105 70 67 LEU H H 9.0360 . 1 106 70 67 LEU N N 117.7280 . 1 107 71 68 GLN H H 7.1980 . 1 108 71 68 GLN N N 119.8840 . 1 109 72 69 ALA H H 8.3040 . 1 110 72 69 ALA N N 124.7730 . 1 111 73 70 LEU H H 7.9240 . 1 112 73 70 LEU N N 119.3250 . 1 113 74 71 THR H H 7.8280 . 1 114 74 71 THR N N 120.6710 . 1 115 75 72 LEU H H 7.5920 . 1 116 75 72 LEU N N 124.4160 . 1 117 76 73 LEU H H 8.7170 . 1 118 76 73 LEU N N 121.5870 . 1 119 77 74 GLY H H 8.5570 . 1 120 77 74 GLY N N 104.4220 . 1 121 78 75 ALA H H 8.0230 . 1 122 78 75 ALA N N 126.2920 . 1 123 79 76 CYS H H 8.4230 . 1 124 79 76 CYS N N 119.2730 . 1 125 81 78 ALA H H 7.6020 . 1 126 81 78 ALA N N 119.8770 . 1 127 82 79 ASN H H 7.9060 . 1 128 82 79 ASN N N 112.4900 . 1 129 83 80 CYS H H 8.1010 . 1 130 83 80 CYS N N 116.3360 . 1 131 84 81 GLY H H 8.0850 . 1 132 84 81 GLY N N 107.9700 . 1 133 85 82 LYS H H 8.4790 . 1 134 85 82 LYS N N 121.4110 . 1 135 86 83 ILE H H 8.3670 . 1 136 86 83 ILE N N 117.4420 . 1 137 87 84 PHE H H 7.1630 . 1 138 87 84 PHE N N 121.0100 . 1 139 88 85 ARG H H 7.5870 . 1 140 88 85 ARG N N 119.6860 . 1 141 89 86 LEU H H 7.8570 . 1 142 89 86 LEU N N 119.3490 . 1 143 90 87 GLY H H 6.8920 . 1 144 90 87 GLY N N 105.1790 . 1 145 91 88 VAL H H 6.3880 . 1 146 91 88 VAL N N 115.4670 . 1 147 92 89 CYS H H 6.7780 . 1 148 92 89 CYS N N 116.5310 . 1 149 93 90 SER H H 7.0420 . 1 150 93 90 SER N N 114.2720 . 1 151 94 91 ARG H H 9.0160 . 1 152 94 91 ARG N N 124.7670 . 1 153 95 92 ASP H H 8.3090 . 1 154 95 92 ASP N N 119.4980 . 1 155 96 93 PHE H H 7.7430 . 1 156 96 93 PHE N N 123.2370 . 1 157 97 94 ALA H H 7.6670 . 1 158 97 94 ALA N N 121.7130 . 1 159 98 95 THR H H 8.0570 . 1 160 98 95 THR N N 114.6060 . 1 161 99 96 GLU H H 7.5280 . 1 162 99 96 GLU N N 124.1210 . 1 163 100 97 VAL H H 8.2710 . 1 164 100 97 VAL N N 117.8660 . 1 165 101 98 ARG H H 7.5870 . 1 166 101 98 ARG N N 119.6860 . 1 167 102 99 ALA H H 7.3300 . 1 168 102 99 ALA N N 121.3700 . 1 169 103 100 VAL H H 7.6640 . 1 170 103 100 VAL N N 119.4640 . 1 171 104 101 ILE H H 7.3650 . 1 172 104 101 ILE N N 117.9420 . 1 173 105 102 LYS H H 8.3950 . 1 174 105 102 LYS N N 121.0020 . 1 175 106 103 ASN H H 7.8810 . 1 176 106 103 ASN N N 116.9320 . 1 177 107 104 LYS H H 7.5820 . 1 178 107 104 LYS N N 116.3220 . 1 179 108 105 ALA H H 7.4660 . 1 180 108 105 ALA N N 122.0630 . 1 181 109 106 HIS H H 8.5690 . 1 182 109 106 HIS N N 119.4320 . 1 183 111 108 LYS H H 10.0100 . 1 184 111 108 LYS N N 119.2520 . 1 185 112 109 VAL H H 7.3430 . 1 186 112 109 VAL N N 121.9660 . 1 187 113 110 CYS H H 7.6920 . 1 188 113 110 CYS N N 117.3980 . 1 189 114 111 GLU H H 8.2710 . 1 190 114 111 GLU N N 117.8660 . 1 191 115 112 LYS H H 7.4480 . 1 192 115 112 LYS N N 121.4150 . 1 193 117 114 LYS H H 7.9600 . 1 194 117 114 LYS N N 117.3630 . 1 195 118 115 SER H H 7.8890 . 1 196 118 115 SER N N 114.1910 . 1 197 120 117 MET H H 7.3200 . 1 198 120 117 MET N N 114.7040 . 1 199 122 119 GLU H H 8.0290 . 1 200 122 119 GLU N N 122.9470 . 1 201 123 120 TRP H H 8.8110 . 1 202 123 120 TRP N N 122.0180 . 1 203 124 121 SER H H 8.2630 . 1 204 124 121 SER N N 113.8730 . 1 205 125 122 GLU H H 7.3800 . 1 206 125 122 GLU N N 120.0390 . 1 207 128 125 GLN H H 6.9180 . 1 208 128 125 GLN N N 117.6450 . 1 209 129 126 LYS H H 8.1290 . 1 210 129 126 LYS N N 117.2140 . 1 211 130 127 ASP H H 7.7430 . 1 212 130 127 ASP N N 122.0940 . 1 213 132 129 GLN H H 8.4230 . 1 214 132 129 GLN N N 117.8890 . 1 215 133 130 PHE H H 7.7930 . 1 216 133 130 PHE N N 118.7470 . 1 217 134 131 SER H H 7.4510 . 1 218 134 131 SER N N 117.0120 . 1 219 135 132 LEU H H 8.1070 . 1 220 135 132 LEU N N 123.7080 . 1 221 136 133 ILE H H 7.3430 . 1 222 136 133 ILE N N 121.9660 . 1 223 138 135 ALA H H 7.9890 . 1 224 138 135 ALA N N 125.0560 . 1 225 139 136 THR H H 8.0890 . 1 226 139 136 THR N N 119.4820 . 1 227 140 137 ILE H H 7.8690 . 1 228 140 137 ILE N N 123.2760 . 1 229 141 138 LYS H H 7.5020 . 1 230 141 138 LYS N N 119.2000 . 1 231 142 139 SER H H 7.8620 . 1 232 142 139 SER N N 114.2810 . 1 233 144 141 LYS H H 8.7140 . 1 234 144 141 LYS N N 122.8390 . 1 235 145 142 GLU H H 7.7510 . 1 236 145 142 GLU N N 121.3640 . 1 237 146 143 GLU H H 7.3190 . 1 238 146 143 GLU N N 117.7180 . 1 239 147 144 GLY H H 7.7260 . 1 240 147 144 GLY N N 107.9540 . 1 241 148 145 ILE H H 7.6650 . 1 242 148 145 ILE N N 124.0110 . 1 243 149 146 THR H H 8.1200 . 1 244 149 146 THR N N 120.7970 . 1 245 150 147 PHE H H 8.8220 . 1 246 150 147 PHE N N 123.1780 . 1 247 153 150 ALA H H 8.2670 . 1 248 153 150 ALA N N 124.8270 . 1 249 154 151 GLY H H 8.8610 . 1 250 154 151 GLY N N 110.9960 . 1 251 155 152 SER H H 8.0630 . 1 252 155 152 SER N N 116.0090 . 1 253 156 153 GLN H H 8.3370 . 1 254 156 153 GLN N N 122.6970 . 1 255 157 154 THR H H 8.0440 . 1 256 157 154 THR N N 117.0560 . 1 257 158 155 VAL H H 8.0820 . 1 258 158 155 VAL N N 123.9160 . 1 259 159 156 SER H H 8.2430 . 1 260 159 156 SER N N 120.5100 . 1 261 160 157 ALA H H 8.2050 . 1 262 160 157 ALA N N 127.1790 . 1 263 161 158 ALA H H 7.9420 . 1 264 161 158 ALA N N 123.0970 . 1 265 162 159 ALA H H 7.8950 . 1 266 162 159 ALA N N 123.4730 . 1 267 163 160 LYS H H 8.0110 . 1 268 163 160 LYS N N 121.0260 . 1 269 164 161 ASN H H 8.1830 . 1 270 164 161 ASN N N 119.8380 . 1 271 165 162 GLY H H 8.1560 . 1 272 165 162 GLY N N 109.9650 . 1 273 166 163 THR H H 7.9550 . 1 274 166 163 THR N N 114.1120 . 1 275 167 164 SER H H 8.2520 . 1 276 167 164 SER N N 118.7760 . 1 277 168 165 SER H H 8.2520 . 1 278 168 165 SER N N 118.7760 . 1 279 169 166 ASN H H 8.2780 . 1 280 169 166 ASN N N 121.6440 . 1 281 170 167 LYS H H 8.1440 . 1 282 170 167 LYS N N 122.9370 . 1 283 171 168 ASN H H 8.3590 . 1 284 171 168 ASN N N 120.4820 . 1 285 172 169 LYS H H 8.1440 . 1 286 172 169 LYS N N 122.9370 . 1 287 173 170 GLU H H 8.3240 . 1 288 173 170 GLU N N 121.5300 . 1 289 174 171 ASP H H 8.1200 . 1 290 174 171 ASP N N 120.7970 . 1 291 175 172 GLU H H 8.0710 . 1 292 175 172 GLU N N 122.4470 . 1 293 176 173 ASP H H 8.2430 . 1 294 176 173 ASP N N 122.0590 . 1 295 177 174 ILE H H 7.8690 . 1 296 177 174 ILE N N 123.2760 . 1 297 178 175 ALA H H 7.9070 . 1 298 178 175 ALA N N 125.0300 . 1 299 179 176 LYS H H 7.9420 . 1 300 179 176 LYS N N 120.3230 . 1 301 180 177 ALA H H 7.7880 . 1 302 180 177 ALA N N 124.2700 . 1 303 181 178 ILE H H 7.9270 . 1 304 181 178 ILE N N 121.2850 . 1 305 182 179 GLU H H 7.9850 . 1 306 182 179 GLU N N 123.6580 . 1 307 183 180 LEU H H 8.0710 . 1 308 183 180 LEU N N 122.2930 . 1 309 184 181 SER H H 7.9910 . 1 310 184 181 SER N N 116.8080 . 1 311 185 182 LEU H H 7.8950 . 1 312 185 182 LEU N N 123.4730 . 1 313 186 183 GLN H H 7.8720 . 1 314 186 183 GLN N N 120.0100 . 1 315 187 184 GLU H H 8.0310 . 1 316 187 184 GLU N N 121.4080 . 1 317 188 185 GLN H H 8.0570 . 1 318 188 185 GLN N N 121.1160 . 1 319 189 186 LYS H H 7.9810 . 1 320 189 186 LYS N N 122.0230 . 1 321 190 187 GLN H H 8.1360 . 1 322 190 187 GLN N N 121.7240 . 1 323 191 188 GLN H H 8.1360 . 1 324 191 188 GLN N N 121.7240 . 1 325 193 190 THR H H 8.0400 . 1 326 193 190 THR N N 117.2820 . 1 327 194 191 GLU H H 8.4390 . 1 328 194 191 GLU N N 124.8370 . 1 329 195 192 THR H H 7.7080 . 1 330 195 192 THR N N 121.1470 . 1 stop_ save_