data_18371 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for EB1 C-terminal domain ; _BMRB_accession_number 18371 _BMRB_flat_file_name bmr18371.str _Entry_type original _Submission_date 2012-03-30 _Accession_date 2012-03-30 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kanaba Teppei . . 2 Mishima Masaki . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 67 "13C chemical shifts" 177 "15N chemical shifts" 67 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2013-02-11 update BMRB 'update entry citation' 2012-11-15 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 18348 'EB1 CH domain' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Microtubule-binding sites of the CH domain of EB1 and its autoinhibition revealed by NMR.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 23128140 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kanaba Teppei . . 2 Maesaki Ryoko . . 3 Mori Tomoyuki . . 4 Ito Yutaka . . 5 Hakoshima Toshio . . 6 Mishima Masaki . . stop_ _Journal_abbreviation 'Biochim. Biophys. Acta' _Journal_name_full 'Biochimica et biophysica acta' _Journal_volume 1834 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 499 _Page_last 507 _Year 2013 _Details . loop_ _Keyword EB1 microtubule stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'EB1 C-terminal domain' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'EB1 C-terminal domain' $EB1_C-terminal_domain stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_EB1_C-terminal_domain _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common EB1_C-terminal_domain _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 85 _Mol_residue_sequence ; GPLGSGDDEAAELMQQVNVL KLTVEDLEKERDFYFGKLRN IELICQENEGENDPVLQRIV DILYATDEGFVIPDEGGPQE EQEEY ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 184 GLY 2 185 PRO 3 186 LEU 4 187 GLY 5 188 SER 6 189 GLY 7 190 ASP 8 191 ASP 9 192 GLU 10 193 ALA 11 194 ALA 12 195 GLU 13 196 LEU 14 197 MET 15 198 GLN 16 199 GLN 17 200 VAL 18 201 ASN 19 202 VAL 20 203 LEU 21 204 LYS 22 205 LEU 23 206 THR 24 207 VAL 25 208 GLU 26 209 ASP 27 210 LEU 28 211 GLU 29 212 LYS 30 213 GLU 31 214 ARG 32 215 ASP 33 216 PHE 34 217 TYR 35 218 PHE 36 219 GLY 37 220 LYS 38 221 LEU 39 222 ARG 40 223 ASN 41 224 ILE 42 225 GLU 43 226 LEU 44 227 ILE 45 228 CYS 46 229 GLN 47 230 GLU 48 231 ASN 49 232 GLU 50 233 GLY 51 234 GLU 52 235 ASN 53 236 ASP 54 237 PRO 55 238 VAL 56 239 LEU 57 240 GLN 58 241 ARG 59 242 ILE 60 243 VAL 61 244 ASP 62 245 ILE 63 246 LEU 64 247 TYR 65 248 ALA 66 249 THR 67 250 ASP 68 251 GLU 69 252 GLY 70 253 PHE 71 254 VAL 72 255 ILE 73 256 PRO 74 257 ASP 75 258 GLU 76 259 GLY 77 260 GLY 78 261 PRO 79 262 GLN 80 263 GLU 81 264 GLU 82 265 GLN 83 266 GLU 84 267 GLU 85 268 TYR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-05 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1TXQ "Crystal Structure Of The Eb1 C-Terminal Domain Complexed With The Cap-Gly Domain Of P150glued" 97.65 86 97.59 100.00 1.36e-48 PDB 1WU9 "Crystal Structure Of The C-Terminal Domain Of The End- Binding Protein 1 (Eb1)" 94.12 80 98.75 98.75 4.90e-47 PDB 2HKQ "Crystal Structure Of The C-Terminal Domain Of Human Eb1 In Complex With The Cap-Gly Domain Of Human Dynactin-1 (P150-Glued)" 94.12 80 98.75 98.75 4.90e-47 PDB 2HL5 "Crystal Structure Of The C-Terminal Domain Of Human Eb1 In Complex With The A49m Mutant Cap-Gly Domain Of Human Dynactin-1 (P15" 94.12 80 98.75 98.75 4.90e-47 PDB 2R8U "Structure Of Fragment Of Human End-Binding Protein 1 (Eb1) Containing The N-Terminal Domain At 1.35 A Resolution" 97.65 268 97.59 100.00 3.62e-48 PDB 3GJO "Crystal Structure Of Human Eb1 In Complex With Microtubule Tip Localization Signal Peptide Of Macf" 84.71 72 98.61 98.61 2.19e-41 PDB 3TQ7 "Eb1cEB3C HETERODIMER IN COMPLEX WITH THE CAP-Gly Domain Of P150glued" 91.76 78 100.00 100.00 3.96e-46 DBJ BAE89438 "unnamed protein product [Macaca fascicularis]" 97.65 268 97.59 100.00 3.28e-48 DBJ BAG35484 "unnamed protein product [Homo sapiens]" 97.65 268 97.59 100.00 3.62e-48 DBJ BAG59745 "unnamed protein product [Homo sapiens]" 97.65 238 97.59 100.00 1.30e-48 DBJ BAG73401 "microtubule-associated protein, RP/EB family, member 1 [synthetic construct]" 97.65 268 97.59 100.00 3.62e-48 EMBL CAH92115 "hypothetical protein [Pongo abelii]" 97.65 268 97.59 100.00 3.50e-48 GB AAC09471 "EB1 [Homo sapiens]" 97.65 268 97.59 100.00 3.62e-48 GB AAI06069 "Microtubule-associated protein, RP/EB family, member 1 [Homo sapiens]" 97.65 268 97.59 100.00 3.62e-48 GB AAI09282 "Microtubule-associated protein, RP/EB family, member 1 [Homo sapiens]" 97.65 268 97.59 100.00 3.62e-48 GB AAY18920 "microtubule-associated protein [synthetic construct]" 97.65 292 97.59 100.00 3.92e-48 GB ACE87787 "microtubule-associated protein, RP/EB family, member 1 protein [synthetic construct]" 97.65 268 97.59 100.00 3.62e-48 REF NP_001126236 "microtubule-associated protein RP/EB family member 1 [Pongo abelii]" 97.65 268 97.59 100.00 3.50e-48 REF NP_001253729 "microtubule-associated protein RP/EB family member 1 [Macaca mulatta]" 97.65 268 97.59 100.00 3.14e-48 REF NP_001271003 "uncharacterized protein LOC101865348 [Macaca fascicularis]" 97.65 268 97.59 100.00 3.28e-48 REF NP_036457 "microtubule-associated protein RP/EB family member 1 [Homo sapiens]" 97.65 268 97.59 100.00 3.62e-48 REF XP_002710850 "PREDICTED: microtubule-associated protein RP/EB family member 1 [Oryctolagus cuniculus]" 97.65 268 97.59 100.00 4.34e-48 SP Q15691 "RecName: Full=Microtubule-associated protein RP/EB family member 1; AltName: Full=APC-binding protein EB1; AltName: Full=End-bi" 97.65 268 97.59 100.00 3.62e-48 SP Q5R7Z5 "RecName: Full=Microtubule-associated protein RP/EB family member 1; AltName: Full=APC-binding protein EB1; AltName: Full=End-bi" 97.65 268 97.59 100.00 3.50e-48 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $EB1_C-terminal_domain Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $EB1_C-terminal_domain 'recombinant technology' . Escherichia coli . pGEX6P-3 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $EB1_C-terminal_domain 1.0 mM '[U-100% 13C; U-100% 15N]' H2O 93 % 'natural abundance' D2O 7 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCANH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCANH' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HN(CA)CO_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)CO' _Sample_label $sample_1 save_ save_3D_HNCO_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.4 . M pH 7.0 . pH pressure 1 . atm temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCANH' '3D CBCA(CO)NH' '3D HNCA' '3D HN(CO)CA' '3D HN(CA)CO' '3D HNCO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'EB1 C-terminal domain' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 190 7 ASP H H 8.322 0.04 1 2 190 7 ASP C C 176.261 0.40 1 3 190 7 ASP CA C 54.673 0.40 1 4 190 7 ASP CB C 41.160 0.40 1 5 190 7 ASP N N 122.469 0.40 1 6 191 8 ASP H H 8.284 0.04 1 7 191 8 ASP C C 176.790 0.40 1 8 191 8 ASP CA C 54.505 0.40 1 9 191 8 ASP CB C 41.112 0.40 1 10 191 8 ASP N N 122.390 0.40 1 11 193 10 ALA H H 8.344 0.04 1 12 193 10 ALA C C 178.737 0.40 1 13 193 10 ALA CA C 54.416 0.40 1 14 193 10 ALA CB C 17.798 0.40 1 15 193 10 ALA N N 123.745 0.40 1 16 194 11 ALA H H 7.968 0.04 1 17 194 11 ALA C C 180.572 0.40 1 18 194 11 ALA CA C 55.013 0.40 1 19 194 11 ALA CB C 17.955 0.40 1 20 194 11 ALA N N 122.142 0.40 1 21 195 12 GLU H H 7.893 0.04 1 22 195 12 GLU C C 179.431 0.40 1 23 195 12 GLU CA C 58.555 0.40 1 24 195 12 GLU CB C 29.225 0.40 1 25 195 12 GLU N N 121.050 0.40 1 26 196 13 LEU H H 8.105 0.04 1 27 196 13 LEU C C 178.785 0.40 1 28 196 13 LEU CA C 58.326 0.40 1 29 196 13 LEU CB C 43.047 0.40 1 30 196 13 LEU N N 123.103 0.40 1 31 197 14 MET H H 8.443 0.04 1 32 197 14 MET C C 178.481 0.40 1 33 197 14 MET CA C 58.192 0.40 1 34 197 14 MET CB C 32.169 0.40 1 35 197 14 MET N N 120.043 0.40 1 36 198 15 GLN H H 7.596 0.04 1 37 198 15 GLN C C 178.151 0.40 1 38 198 15 GLN CA C 58.862 0.40 1 39 198 15 GLN CB C 27.581 0.40 1 40 198 15 GLN N N 120.118 0.40 1 41 199 16 GLN H H 7.804 0.04 1 42 199 16 GLN CA C 59.122 0.40 1 43 199 16 GLN CB C 28.115 0.40 1 44 199 16 GLN N N 121.292 0.40 1 45 200 17 VAL H H 8.344 0.04 1 46 200 17 VAL C C 177.127 0.40 1 47 200 17 VAL CA C 67.557 0.40 1 48 200 17 VAL CB C 30.115 0.40 1 49 200 17 VAL N N 121.572 0.40 1 50 201 18 ASN H H 7.990 0.04 1 51 201 18 ASN C C 179.678 0.40 1 52 201 18 ASN CA C 57.317 0.40 1 53 201 18 ASN CB C 39.976 0.40 1 54 201 18 ASN N N 120.015 0.40 1 55 202 19 VAL H H 7.274 0.04 1 56 202 19 VAL C C 178.517 0.40 1 57 202 19 VAL CA C 63.862 0.40 1 58 202 19 VAL N N 121.322 0.40 1 59 204 21 LYS H H 8.736 0.04 1 60 204 21 LYS C C 179.834 0.40 1 61 204 21 LYS CA C 60.899 0.40 1 62 204 21 LYS CB C 32.228 0.40 1 63 204 21 LYS N N 121.171 0.40 1 64 205 22 LEU H H 7.505 0.04 1 65 205 22 LEU C C 178.750 0.40 1 66 205 22 LEU CA C 58.101 0.40 1 67 205 22 LEU CB C 41.201 0.40 1 68 205 22 LEU N N 121.992 0.40 1 69 208 25 GLU H H 7.572 0.04 1 70 208 25 GLU C C 179.521 0.40 1 71 208 25 GLU CA C 59.626 0.40 1 72 208 25 GLU CB C 29.484 0.40 1 73 208 25 GLU N N 120.135 0.40 1 74 209 26 ASP H H 8.066 0.04 1 75 209 26 ASP CA C 57.463 0.40 1 76 209 26 ASP CB C 40.426 0.40 1 77 209 26 ASP N N 121.710 0.40 1 78 211 28 GLU H H 8.895 0.04 1 79 211 28 GLU C C 178.265 0.40 1 80 211 28 GLU CA C 60.586 0.40 1 81 211 28 GLU CB C 29.728 0.40 1 82 211 28 GLU N N 121.796 0.40 1 83 212 29 LYS H H 7.534 0.04 1 84 212 29 LYS C C 179.640 0.40 1 85 212 29 LYS CA C 59.801 0.40 1 86 212 29 LYS CB C 32.175 0.40 1 87 212 29 LYS N N 119.139 0.40 1 88 213 30 GLU H H 7.864 0.04 1 89 213 30 GLU C C 177.440 0.40 1 90 213 30 GLU CA C 59.743 0.40 1 91 213 30 GLU N N 123.408 0.40 1 92 214 31 ARG H H 8.968 0.04 1 93 214 31 ARG C C 178.736 0.40 1 94 214 31 ARG CA C 61.083 0.40 1 95 214 31 ARG N N 120.818 0.40 1 96 215 32 ASP H H 8.800 0.04 1 97 215 32 ASP C C 178.996 0.40 1 98 215 32 ASP CA C 57.521 0.40 1 99 215 32 ASP CB C 39.465 0.40 1 100 215 32 ASP N N 120.853 0.40 1 101 216 33 PHE H H 8.184 0.04 1 102 216 33 PHE CA C 60.204 0.40 1 103 216 33 PHE CB C 39.078 0.40 1 104 216 33 PHE N N 126.548 0.40 1 105 217 34 TYR H H 8.149 0.04 1 106 217 34 TYR C C 178.096 0.40 1 107 217 34 TYR CA C 58.584 0.40 1 108 217 34 TYR N N 120.308 0.40 1 109 218 35 PHE H H 9.130 0.04 1 110 218 35 PHE C C 177.350 0.40 1 111 218 35 PHE CA C 61.685 0.40 1 112 218 35 PHE N N 122.189 0.40 1 113 219 36 GLY H H 8.433 0.04 1 114 219 36 GLY C C 176.660 0.40 1 115 219 36 GLY CA C 46.921 0.40 1 116 219 36 GLY N N 107.122 0.40 1 117 220 37 LYS H H 7.197 0.04 1 118 220 37 LYS C C 178.306 0.40 1 119 220 37 LYS CA C 60.556 0.40 1 120 220 37 LYS N N 122.386 0.40 1 121 221 38 LEU H H 7.502 0.04 1 122 221 38 LEU CA C 58.160 0.40 1 123 221 38 LEU CB C 40.281 0.40 1 124 221 38 LEU N N 120.660 0.40 1 125 222 39 ARG H H 8.121 0.04 1 126 222 39 ARG CA C 58.313 0.40 1 127 222 39 ARG CB C 29.052 0.40 1 128 222 39 ARG N N 121.096 0.40 1 129 223 40 ASN H H 7.736 0.04 1 130 223 40 ASN CA C 56.190 0.40 1 131 223 40 ASN CB C 38.413 0.40 1 132 223 40 ASN N N 119.851 0.40 1 133 224 41 ILE H H 8.319 0.04 1 134 224 41 ILE C C 177.180 0.40 1 135 224 41 ILE CA C 55.952 0.40 1 136 224 41 ILE CB C 38.254 0.40 1 137 224 41 ILE N N 124.166 0.40 1 138 225 42 GLU H H 8.573 0.04 1 139 225 42 GLU C C 178.242 0.40 1 140 225 42 GLU CA C 60.372 0.40 1 141 225 42 GLU CB C 29.260 0.40 1 142 225 42 GLU N N 124.773 0.40 1 143 226 43 LEU H H 7.644 0.04 1 144 226 43 LEU C C 179.612 0.40 1 145 226 43 LEU CA C 58.374 0.40 1 146 226 43 LEU CB C 41.524 0.40 1 147 226 43 LEU N N 120.147 0.40 1 148 227 44 ILE H H 7.374 0.04 1 149 227 44 ILE C C 179.219 0.40 1 150 227 44 ILE CA C 64.968 0.40 1 151 227 44 ILE CB C 37.400 0.40 1 152 227 44 ILE N N 121.683 0.40 1 153 228 45 CYS H H 8.092 0.04 1 154 228 45 CYS C C 177.341 0.40 1 155 228 45 CYS CA C 64.789 0.40 1 156 228 45 CYS CB C 26.327 0.40 1 157 228 45 CYS N N 120.308 0.40 1 158 229 46 GLN H H 8.278 0.04 1 159 229 46 GLN C C 178.526 0.40 1 160 229 46 GLN CA C 58.385 0.40 1 161 229 46 GLN CB C 28.323 0.40 1 162 229 46 GLN N N 121.159 0.40 1 163 230 47 GLU H H 7.915 0.04 1 164 230 47 GLU CA C 58.274 0.40 1 165 230 47 GLU CB C 29.739 0.40 1 166 230 47 GLU N N 120.804 0.40 1 167 231 48 ASN H H 7.349 0.04 1 168 231 48 ASN C C 174.583 0.40 1 169 231 48 ASN CA C 53.454 0.40 1 170 231 48 ASN CB C 40.446 0.40 1 171 231 48 ASN N N 117.480 0.40 1 172 232 49 GLU H H 7.734 0.04 1 173 232 49 GLU C C 177.415 0.40 1 174 232 49 GLU CA C 58.237 0.40 1 175 232 49 GLU CB C 29.350 0.40 1 176 232 49 GLU N N 123.603 0.40 1 177 233 50 GLY H H 8.593 0.04 1 178 233 50 GLY C C 174.303 0.40 1 179 233 50 GLY CA C 45.980 0.40 1 180 233 50 GLY N N 110.691 0.40 1 181 234 51 GLU H H 7.919 0.04 1 182 234 51 GLU C C 176.498 0.40 1 183 234 51 GLU CA C 56.484 0.40 1 184 234 51 GLU CB C 29.492 0.40 1 185 234 51 GLU N N 120.259 0.40 1 186 235 52 ASN H H 8.249 0.04 1 187 235 52 ASN C C 174.451 0.40 1 188 235 52 ASN CA C 53.382 0.40 1 189 235 52 ASN CB C 38.115 0.40 1 190 235 52 ASN N N 119.689 0.40 1 191 236 53 ASP H H 8.320 0.04 1 192 236 53 ASP CA C 52.784 0.40 1 193 236 53 ASP CB C 42.695 0.40 1 194 236 53 ASP N N 124.238 0.40 1 195 238 55 VAL H H 8.065 0.04 1 196 238 55 VAL C C 177.863 0.40 1 197 238 55 VAL CA C 66.409 0.40 1 198 238 55 VAL N N 121.774 0.40 1 199 239 56 LEU H H 7.803 0.04 1 200 239 56 LEU C C 179.441 0.40 1 201 239 56 LEU CA C 57.801 0.40 1 202 239 56 LEU CB C 40.652 0.40 1 203 239 56 LEU N N 121.064 0.40 1 204 240 57 GLN H H 8.185 0.04 1 205 240 57 GLN C C 177.763 0.40 1 206 240 57 GLN CA C 58.603 0.40 1 207 240 57 GLN CB C 27.959 0.40 1 208 240 57 GLN N N 118.407 0.40 1 209 241 58 ARG H H 7.323 0.04 1 210 241 58 ARG C C 179.335 0.40 1 211 241 58 ARG CA C 59.041 0.40 1 212 241 58 ARG CB C 30.385 0.40 1 213 241 58 ARG N N 119.699 0.40 1 214 244 61 ASP H H 7.817 0.04 1 215 244 61 ASP C C 178.227 0.40 1 216 244 61 ASP CA C 56.575 0.40 1 217 244 61 ASP CB C 37.863 0.40 1 218 244 61 ASP N N 119.189 0.40 1 219 245 62 ILE H H 8.085 0.04 1 220 245 62 ILE CA C 57.122 0.40 1 221 245 62 ILE CB C 38.007 0.40 1 222 245 62 ILE N N 121.753 0.40 1 223 247 64 TYR H H 7.761 0.04 1 224 247 64 TYR C C 175.925 0.40 1 225 247 64 TYR CA C 56.943 0.40 1 226 247 64 TYR CB C 37.476 0.40 1 227 247 64 TYR N N 116.445 0.40 1 228 248 65 ALA H H 7.304 0.04 1 229 248 65 ALA C C 178.805 0.40 1 230 248 65 ALA CA C 53.405 0.40 1 231 248 65 ALA CB C 18.924 0.40 1 232 248 65 ALA N N 125.876 0.40 1 233 249 66 THR H H 8.566 0.04 1 234 249 66 THR C C 174.276 0.40 1 235 249 66 THR CA C 61.182 0.40 1 236 249 66 THR CB C 70.446 0.40 1 237 249 66 THR N N 115.815 0.40 1 238 250 67 ASP H H 8.322 0.04 1 239 250 67 ASP C C 176.234 0.40 1 240 250 67 ASP CA C 54.240 0.40 1 241 250 67 ASP CB C 41.213 0.40 1 242 250 67 ASP N N 123.263 0.40 1 243 251 68 GLU H H 8.410 0.04 1 244 251 68 GLU C C 176.854 0.40 1 245 251 68 GLU CA C 57.102 0.40 1 246 251 68 GLU CB C 29.930 0.40 1 247 251 68 GLU N N 123.385 0.40 1 248 252 69 GLY H H 8.351 0.04 1 249 252 69 GLY C C 173.554 0.40 1 250 252 69 GLY CA C 45.225 0.40 1 251 252 69 GLY N N 110.970 0.40 1 252 253 70 PHE H H 7.848 0.04 1 253 253 70 PHE C C 174.963 0.40 1 254 253 70 PHE CA C 57.629 0.40 1 255 253 70 PHE CB C 39.582 0.40 1 256 253 70 PHE N N 121.784 0.40 1 257 254 71 VAL H H 7.897 0.04 1 258 254 71 VAL CA C 61.774 0.40 1 259 254 71 VAL CB C 32.943 0.40 1 260 254 71 VAL N N 125.270 0.40 1 261 255 72 ILE H H 8.245 0.04 1 262 255 72 ILE C C 174.480 0.40 1 263 255 72 ILE CA C 58.569 0.40 1 264 255 72 ILE CB C 38.373 0.40 1 265 255 72 ILE N N 129.329 0.40 1 266 257 74 ASP H H 8.296 0.04 1 267 257 74 ASP C C 176.356 0.40 1 268 257 74 ASP CA C 54.405 0.40 1 269 257 74 ASP CB C 41.184 0.40 1 270 257 74 ASP N N 122.883 0.40 1 271 258 75 GLU H H 8.370 0.04 1 272 258 75 GLU C C 176.889 0.40 1 273 258 75 GLU CA C 56.602 0.40 1 274 258 75 GLU CB C 30.152 0.40 1 275 258 75 GLU N N 123.730 0.40 1 276 259 76 GLY H H 8.426 0.04 1 277 259 76 GLY C C 174.284 0.40 1 278 259 76 GLY CA C 45.195 0.40 1 279 259 76 GLY N N 111.591 0.40 1 280 260 77 GLY H H 8.076 0.04 1 281 260 77 GLY C C 171.671 0.40 1 282 260 77 GLY CA C 44.544 0.40 1 283 260 77 GLY N N 110.654 0.40 1 284 262 79 GLN H H 8.489 0.04 1 285 262 79 GLN C C 175.734 0.40 1 286 262 79 GLN CA C 55.529 0.40 1 287 262 79 GLN CB C 29.431 0.40 1 288 262 79 GLN N N 122.880 0.40 1 289 263 80 GLU H H 8.353 0.04 1 290 263 80 GLU C C 175.375 0.40 1 291 263 80 GLU CA C 56.288 0.40 1 292 263 80 GLU CB C 30.209 0.40 1 293 263 80 GLU N N 124.654 0.40 1 294 264 81 GLU H H 7.987 0.04 1 295 264 81 GLU C C 180.983 0.40 1 296 264 81 GLU CA C 58.044 0.40 1 297 264 81 GLU CB C 30.934 0.40 1 298 264 81 GLU N N 129.036 0.40 1 299 266 83 GLU H H 8.394 0.04 1 300 266 83 GLU CA C 56.174 0.40 1 301 266 83 GLU CB C 30.376 0.40 1 302 266 83 GLU N N 124.671 0.40 1 303 267 84 GLU H H 8.263 0.04 1 304 267 84 GLU C C 174.885 0.40 1 305 267 84 GLU CA C 56.455 0.40 1 306 267 84 GLU CB C 30.396 0.40 1 307 267 84 GLU N N 123.940 0.40 1 308 268 85 TYR H H 7.681 0.04 1 309 268 85 TYR CA C 59.049 0.40 1 310 268 85 TYR CB C 39.405 0.40 1 311 268 85 TYR N N 127.769 0.40 1 stop_ save_