data_25365 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for drosophila histone mRNA stem-loop-binding protein ; _BMRB_accession_number 25365 _BMRB_flat_file_name bmr25365.str _Entry_type original _Submission_date 2014-11-24 _Accession_date 2014-11-24 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Zhang Jun . . 2 DeRose Eugene . . 3 Hall Traci . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 37 "13C chemical shifts" 92 "15N chemical shifts" 37 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-12-09 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 25364 'drosophila stem-loop binding protein complexed with histone mRNA stem-loop' stop_ _Original_release_date 2014-11-24 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Molecular mechanisms for the regulation of histone mRNA stem-loop binding protein by phosphorylation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 25002523 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Zhang Jun . . 2 Tan Dazhi . . 3 DeRose Eugene F. . 4 Perera Lalith . . 5 Dominski Zbigniew . . 6 Marzluff William F. . 7 Tong Liang . . 8 Hall Traci . . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_volume 111 _Journal_issue 29 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first E2937 _Page_last E2946 _Year 2014 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name SLBP _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label SLBP $SLBP stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_SLBP _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common SLBP _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 93 _Mol_residue_sequence ; SSSYTEADPAILSRRQKQID YGKNTAAYERYVEMVPKDER TRDHPREPNKYGKYSRRAFD GLVKIWRKSLHIYDPPTQAR DTAKDENEDEDED ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 184 SER 2 185 SER 3 186 SER 4 187 TYR 5 188 THR 6 189 GLU 7 190 ALA 8 191 ASP 9 192 PRO 10 193 ALA 11 194 ILE 12 195 LEU 13 196 SER 14 197 ARG 15 198 ARG 16 199 GLN 17 200 LYS 18 201 GLN 19 202 ILE 20 203 ASP 21 204 TYR 22 205 GLY 23 206 LYS 24 207 ASN 25 208 THR 26 209 ALA 27 210 ALA 28 211 TYR 29 212 GLU 30 213 ARG 31 214 TYR 32 215 VAL 33 216 GLU 34 217 MET 35 218 VAL 36 219 PRO 37 220 LYS 38 221 ASP 39 222 GLU 40 223 ARG 41 224 THR 42 225 ARG 43 226 ASP 44 227 HIS 45 228 PRO 46 229 ARG 47 230 GLU 48 231 PRO 49 232 ASN 50 233 LYS 51 234 TYR 52 235 GLY 53 236 LYS 54 237 TYR 55 238 SER 56 239 ARG 57 240 ARG 58 241 ALA 59 242 PHE 60 243 ASP 61 244 GLY 62 245 LEU 63 246 VAL 64 247 LYS 65 248 ILE 66 249 TRP 67 250 ARG 68 251 LYS 69 252 SER 70 253 LEU 71 254 HIS 72 255 ILE 73 256 TYR 74 257 ASP 75 258 PRO 76 259 PRO 77 260 THR 78 261 GLN 79 262 ALA 80 263 ARG 81 264 ASP 82 265 THR 83 266 ALA 84 267 LYS 85 268 ASP 86 269 GLU 87 270 ASN 88 271 GLU 89 272 ASP 90 273 GLU 91 274 ASP 92 275 GLU 93 276 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $SLBP 'fruit fly' 7227 Eukaryota Metazoa Drosophila melanogaster stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $SLBP 'recombinant technology' . Escherichia coli . PSMT3 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SLBP 0.5 mM '[U-100% 13C; U-100% 15N]' Hepes 20 mM 'natural abundance' NaCl 500 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 500 . mM pH 7.5 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name SLBP _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 187 4 TYR CA C 54.3755 0.31 1 2 187 4 TYR CB C 41.0605 0.5 1 3 188 5 THR CA C 62.0378 0.31 1 4 188 5 THR CB C 69.8787 0.5 1 5 189 6 GLU CA C 56.5324 0.31 1 6 189 6 GLU CB C 30.2433 0.5 1 7 192 9 PRO CA C 63.8907 0.31 1 8 192 9 PRO CB C 32.0791 0.5 1 9 193 10 ALA H H 8.4466 0.01 1 10 193 10 ALA CA C 53.3603 0.31 1 11 193 10 ALA CB C 18.8420 0.5 1 12 193 10 ALA N N 122.5752 0.12 1 13 194 11 ILE H H 7.7802 0.01 1 14 194 11 ILE CA C 61.9408 0.31 1 15 194 11 ILE CB C 38.1994 0.5 1 16 194 11 ILE N N 118.8271 0.12 1 17 195 12 LEU H H 8.0445 0.01 1 18 195 12 LEU CA C 55.9731 0.31 1 19 195 12 LEU CB C 42.0016 0.5 1 20 195 12 LEU N N 123.7672 0.12 1 21 196 13 SER H H 8.1346 0.01 1 22 196 13 SER CA C 58.9838 0.31 1 23 196 13 SER CB C 63.5233 0.5 1 24 196 13 SER N N 115.4001 0.12 1 25 197 14 ARG H H 8.0976 0.01 1 26 197 14 ARG CA C 56.9663 0.31 1 27 197 14 ARG CB C 30.4885 0.5 1 28 197 14 ARG N N 122.1850 0.12 1 29 198 15 ARG H H 8.1831 0.01 1 30 198 15 ARG CA C 56.8081 0.31 1 31 198 15 ARG CB C 30.4582 0.5 1 32 198 15 ARG N N 120.9713 0.12 1 33 199 16 GLN H H 8.2651 0.01 1 34 199 16 GLN CA C 56.4480 0.31 1 35 199 16 GLN CB C 29.4697 0.5 1 36 199 16 GLN N N 120.8106 0.12 1 37 200 17 LYS H H 8.2459 0.01 1 38 200 17 LYS CA C 56.4685 0.31 1 39 200 17 LYS CB C 32.9696 0.5 1 40 200 17 LYS N N 121.7944 0.12 1 41 201 18 GLN H H 8.2716 0.01 1 42 201 18 GLN CA C 56.0384 0.31 1 43 201 18 GLN CB C 29.4177 0.5 1 44 201 18 GLN N N 121.4038 0.12 1 45 202 19 ILE H H 8.1053 0.01 1 46 202 19 ILE CA C 61.4311 0.31 1 47 202 19 ILE CB C 38.8928 0.5 1 48 202 19 ILE N N 122.3259 0.12 1 49 203 20 ASP H H 8.2680 0.01 1 50 203 20 ASP CA C 53.8867 0.31 1 51 203 20 ASP CB C 41.1880 0.5 1 52 203 20 ASP N N 123.7042 0.12 1 53 204 21 TYR H H 8.1821 0.01 1 54 204 21 TYR CA C 58.8768 0.31 1 55 204 21 TYR CB C 38.4696 0.5 1 56 204 21 TYR N N 121.9499 0.12 1 57 205 22 GLY H H 8.4287 0.01 1 58 205 22 GLY CA C 45.5602 0.31 1 59 205 22 GLY N N 109.8595 0.12 1 60 206 23 LYS H H 8.0407 0.01 1 61 206 23 LYS CA C 56.4942 0.31 1 62 206 23 LYS CB C 33.0168 0.5 1 63 206 23 LYS N N 120.6139 0.12 1 64 207 24 ASN H H 8.4496 0.01 1 65 207 24 ASN CA C 53.4192 0.31 1 66 207 24 ASN CB C 38.5701 0.5 1 67 207 24 ASN N N 119.2207 0.12 1 68 208 25 THR H H 8.0399 0.01 1 69 208 25 THR CA C 62.4745 0.31 1 70 208 25 THR CB C 69.7680 0.5 1 71 208 25 THR N N 114.3733 0.12 1 72 209 26 ALA H H 8.3163 0.01 1 73 209 26 ALA CA C 53.0033 0.31 1 74 209 26 ALA CB C 18.9337 0.5 1 75 209 26 ALA N N 125.7002 0.12 1 76 210 27 ALA H H 8.0916 0.01 1 77 210 27 ALA CA C 53.1018 0.31 1 78 210 27 ALA CB C 18.9282 0.5 1 79 210 27 ALA N N 122.0139 0.12 1 80 211 28 TYR H H 7.8673 0.01 1 81 211 28 TYR CA C 58.4633 0.31 1 82 211 28 TYR CB C 38.6468 0.5 1 83 211 28 TYR N N 118.3324 0.12 1 84 212 29 GLU H H 8.1690 0.01 1 85 212 29 GLU CB C 33.0131 0.5 1 86 212 29 GLU N N 121.3232 0.12 1 87 219 36 PRO CA C 63.0318 0.31 1 88 219 36 PRO CB C 30.9393 0.5 1 89 220 37 LYS H H 8.4669 0.01 1 90 220 37 LYS CA C 56.9166 0.31 1 91 220 37 LYS CB C 32.9957 0.5 1 92 220 37 LYS N N 122.0423 0.12 1 93 221 38 ASP H H 8.3813 0.01 1 94 221 38 ASP CA C 54.4086 0.31 1 95 221 38 ASP CB C 41.2165 0.5 1 96 221 38 ASP N N 120.4275 0.12 1 97 222 39 GLU H H 8.1959 0.01 1 98 222 39 GLU CA C 53.4868 0.31 1 99 222 39 GLU CB C 29.0344 0.5 1 100 222 39 GLU N N 118.8591 0.12 1 101 223 40 ARG CA C 56.8122 0.31 1 102 223 40 ARG CB C 30.5296 0.5 1 103 224 41 THR H H 8.0833 0.01 1 104 224 41 THR CA C 62.1116 0.31 1 105 224 41 THR CB C 69.4702 0.5 1 106 224 41 THR N N 114.0051 0.12 1 107 225 42 ARG H H 8.2136 0.01 1 108 225 42 ARG CA C 56.3729 0.31 1 109 225 42 ARG CB C 30.8351 0.5 1 110 225 42 ARG N N 122.7582 0.12 1 111 228 45 PRO CA C 63.2211 0.31 1 112 228 45 PRO CB C 32.2400 0.5 1 113 229 46 ARG H H 8.5668 0.01 1 114 229 46 ARG CA C 56.2426 0.31 1 115 229 46 ARG CB C 30.5403 0.5 1 116 229 46 ARG N N 121.5658 0.12 1 117 230 47 GLU H H 8.4090 0.01 1 118 230 47 GLU CA C 54.4245 0.31 1 119 230 47 GLU CB C 29.9784 0.5 1 120 230 47 GLU N N 122.9195 0.12 1 121 231 48 PRO CA C 63.3766 0.31 1 122 231 48 PRO CB C 32.0772 0.5 1 123 232 49 ASN H H 8.5203 0.01 1 124 232 49 ASN CA C 53.3566 0.31 1 125 232 49 ASN CB C 38.9693 0.5 1 126 232 49 ASN N N 118.3326 0.12 1 127 233 50 LYS H H 8.3771 0.01 1 128 233 50 LYS CA C 56.3971 0.31 1 129 233 50 LYS CB C 30.4693 0.5 1 130 233 50 LYS N N 121.4038 0.12 1 131 234 51 TYR H H 8.4190 0.01 1 132 234 51 TYR CA C 54.4733 0.31 1 133 234 51 TYR CB C 41.3571 0.5 1 134 234 51 TYR N N 121.7274 0.12 1 135 240 57 ARG CA C 56.5324 0.31 1 136 240 57 ARG CB C 30.2456 0.5 1 137 241 58 ALA H H 8.2932 0.01 1 138 241 58 ALA CA C 52.1205 0.31 1 139 241 58 ALA CB C 19.4823 0.5 1 140 241 58 ALA N N 125.0614 0.12 1 141 242 59 PHE H H 8.2578 0.01 1 142 242 59 PHE CA C 56.4697 0.31 1 143 242 59 PHE CB C 41.3822 0.5 1 144 242 59 PHE N N 121.6938 0.12 1 145 259 76 PRO CA C 63.0678 0.31 1 146 259 76 PRO CB C 31.9363 0.5 1 147 260 77 THR H H 8.1731 0.01 1 148 260 77 THR CA C 62.0003 0.31 1 149 260 77 THR CB C 69.8616 0.5 1 150 260 77 THR N N 114.2198 0.12 1 151 261 78 GLN H H 8.3618 0.01 1 152 261 78 GLN CA C 55.6344 0.31 1 153 261 78 GLN CB C 29.5997 0.5 1 154 261 78 GLN N N 122.5689 0.12 1 155 262 79 ALA H H 8.3751 0.01 1 156 262 79 ALA CA C 52.5850 0.31 1 157 262 79 ALA CB C 19.2408 0.5 1 158 262 79 ALA N N 125.8753 0.12 1 159 263 80 ARG H H 8.3239 0.01 1 160 263 80 ARG CA C 56.2559 0.31 1 161 263 80 ARG CB C 30.7838 0.5 1 162 263 80 ARG N N 120.4363 0.12 1 163 264 81 ASP H H 8.0734 0.01 1 164 264 81 ASP CA C 55.9876 0.31 1 165 264 81 ASP CB C 42.1016 0.5 1 166 264 81 ASP N N 127.0807 0.12 1 stop_ save_