data_25572 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Dad2 C terminal ; _BMRB_accession_number 25572 _BMRB_flat_file_name bmr25572.str _Entry_type original _Submission_date 2015-04-15 _Accession_date 2015-04-15 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Singh Himanshu . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 50 "13C chemical shifts" 115 "15N chemical shifts" 49 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2015-05-15 original BMRB . stop_ _Original_release_date 2015-05-15 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Dad2 C terminal ; _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Singh Himanshu . . stop_ _Journal_abbreviation Biochemistry _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Dad2 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity $entity stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Dad2_C_terminal _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function Signalling stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 139 _Mol_residue_sequence ; MASKTNTAIYQKIAEKRANL ERFREFKELTDDLVLQLESI GDKLETMNGGTASVALILAN WKSVVQSISLASLALMKESN DNNKEAFPEPLVRVRVGQSN EENQDEEEADEEEGVRDSEE VEESTEKLAAALEHHHHHH ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ALA 3 SER 4 LYS 5 THR 6 ASN 7 THR 8 ALA 9 ILE 10 TYR 11 GLN 12 LYS 13 ILE 14 ALA 15 GLU 16 LYS 17 ARG 18 ALA 19 ASN 20 LEU 21 GLU 22 ARG 23 PHE 24 ARG 25 GLU 26 PHE 27 LYS 28 GLU 29 LEU 30 THR 31 ASP 32 ASP 33 LEU 34 VAL 35 LEU 36 GLN 37 LEU 38 GLU 39 SER 40 ILE 41 GLY 42 ASP 43 LYS 44 LEU 45 GLU 46 THR 47 MET 48 ASN 49 GLY 50 GLY 51 THR 52 ALA 53 SER 54 VAL 55 ALA 56 LEU 57 ILE 58 LEU 59 ALA 60 ASN 61 TRP 62 LYS 63 SER 64 VAL 65 VAL 66 GLN 67 SER 68 ILE 69 SER 70 LEU 71 ALA 72 SER 73 LEU 74 ALA 75 LEU 76 MET 77 LYS 78 GLU 79 SER 80 ASN 81 ASP 82 ASN 83 ASN 84 LYS 85 GLU 86 ALA 87 PHE 88 PRO 89 GLU 90 PRO 91 LEU 92 VAL 93 ARG 94 VAL 95 ARG 96 VAL 97 GLY 98 GLN 99 SER 100 ASN 101 GLU 102 GLU 103 ASN 104 GLN 105 ASP 106 GLU 107 GLU 108 GLU 109 ALA 110 ASP 111 GLU 112 GLU 113 GLU 114 GLY 115 VAL 116 ARG 117 ASP 118 SER 119 GLU 120 GLU 121 VAL 122 GLU 123 GLU 124 SER 125 THR 126 GLU 127 LYS 128 LEU 129 ALA 130 ALA 131 ALA 132 LEU 133 GLU 134 HIS 135 HIS 136 HIS 137 HIS 138 HIS 139 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-22 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value EMBL CAX43751 "DASH complex subunit DAD2 homologue, putative [Candida dubliniensis CD36]" 69.78 128 97.94 98.97 3.36e-61 GB EAK95870 "hypothetical protein CaO19.3551 [Candida albicans SC5314]" 89.21 125 100.00 100.00 2.27e-81 GB EEQ45932 "conserved hypothetical protein [Candida albicans WO-1]" 89.21 125 99.19 100.00 7.28e-81 GB KGQ89068 "DASH complex subunit DAD2 [Candida albicans P94015]" 89.21 125 99.19 100.00 7.28e-81 GB KGQ96391 "DASH complex subunit DAD2 [Candida albicans P37005]" 89.21 125 99.19 100.00 7.28e-81 GB KGR00135 "DASH complex subunit DAD2 [Candida albicans GC75]" 89.21 125 99.19 100.00 7.28e-81 REF XP_002418450 "DASH complex subunit DAD2 homologue, putative; DUO1 and DAM1-interacting protein, putative; helper of ASK1 protein, putative; o" 69.78 128 97.94 98.97 3.36e-61 REF XP_714910 "hypothetical protein CaO19.3551 [Candida albicans SC5314]" 89.21 125 100.00 100.00 2.27e-81 SP Q59ZG8 "RecName: Full=DASH complex subunit DAD2; AltName: Full=Outer kinetochore protein DAD2" 89.21 125 100.00 100.00 2.27e-81 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity Nematodes 6239 Eukaryota Metazoa Caenorhabditis elegans stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity 'recombinant technology' . Escherichia coli . pET28 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity .4 mM '[U-100% 15N]' $entity .2 mM '[U-100% 13C]' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_cara _Saveframe_category software _Name cara _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_1H-15N_TOCSY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N TOCSY' _Sample_label $sample_1 save_ save_3D_1H-13C_NOESY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 2 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCO' '3D CBCA(CO)NH' '3D HNCA' '3D 1H-15N NOESY' '3D 1H-15N TOCSY' '3D 1H-13C NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name entity _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 MET H H 8.202 0.020 1 2 1 1 MET C C 175.381 0.3 1 3 1 1 MET CA C 52.633 0.3 1 4 1 1 MET CB C 37.845 0.3 1 5 1 1 MET N N 119.937 0.3 1 6 85 85 GLU H H 8.082 0.020 1 7 85 85 GLU CA C 52.479 0.3 1 8 85 85 GLU CB C 31.921 0.3 1 9 85 85 GLU N N 123.656 0.3 1 10 86 86 ALA H H 8.082 0.020 1 11 86 86 ALA CA C 51.445 0.3 1 12 86 86 ALA CB C 18.610 0.3 1 13 86 86 ALA N N 125.141 0.3 1 14 87 87 PHE H H 8.754 0.020 1 15 87 87 PHE C C 173.913 0.3 1 16 87 87 PHE CA C 52.177 0.3 1 17 87 87 PHE CB C 37.389 0.3 1 18 87 87 PHE N N 118.661 0.3 1 19 93 93 ARG H H 8.134 0.020 1 20 93 93 ARG CA C 55.614 0.3 1 21 93 93 ARG CB C 27.643 0.3 1 22 93 93 ARG N N 120.479 0.3 1 23 94 94 VAL H H 8.070 0.020 1 24 94 94 VAL C C 177.044 0.3 1 25 94 94 VAL CA C 61.460 0.3 1 26 94 94 VAL CB C 31.770 0.3 1 27 94 94 VAL N N 121.451 0.3 1 28 95 95 ARG H H 8.327 0.020 1 29 95 95 ARG CA C 55.270 0.3 1 30 95 95 ARG CB C 30.050 0.3 1 31 95 95 ARG N N 125.279 0.3 1 32 96 96 VAL H H 8.153 0.020 1 33 96 96 VAL C C 175.919 0.3 1 34 96 96 VAL CA C 61.689 0.3 1 35 96 96 VAL CB C 31.884 0.3 1 36 96 96 VAL N N 121.907 0.3 1 37 97 97 GLY H H 8.450 0.020 1 38 97 97 GLY C C 171.277 0.3 1 39 97 97 GLY CA C 42.382 0.3 1 40 97 97 GLY N N 112.766 0.3 1 41 98 98 GLN H H 7.880 0.020 1 42 98 98 GLN N N 118.707 0.3 1 43 99 99 SER H H 8.227 0.020 1 44 99 99 SER C C 174.842 0.3 1 45 99 99 SER CA C 58.594 0.3 1 46 99 99 SER CB C 62.606 0.3 1 47 99 99 SER N N 116.149 0.3 1 48 100 100 ASN H H 8.426 0.020 1 49 100 100 ASN C C 174.549 0.3 1 50 100 100 ASN CA C 52.633 0.3 1 51 100 100 ASN CB C 37.845 0.3 1 52 100 100 ASN N N 120.479 0.3 1 53 101 101 GLU H H 8.077 0.020 1 54 101 101 GLU C C 175.723 0.3 1 55 101 101 GLU CA C 55.843 0.3 1 56 101 101 GLU CB C 27.643 0.3 1 57 101 101 GLU N N 120.064 0.3 1 58 102 102 GLU H H 8.229 0.020 1 59 102 102 GLU CA C 55.384 0.3 1 60 102 102 GLU CB C 27.757 0.3 1 61 102 102 GLU N N 120.399 0.3 1 62 103 103 ASN H H 7.932 0.020 1 63 103 103 ASN C C 173.277 0.3 1 64 103 103 ASN CA C 54.811 0.3 1 65 103 103 ASN CB C 40.596 0.3 1 66 103 103 ASN N N 119.567 0.3 1 67 104 104 GLN H H 7.797 0.020 1 68 104 104 GLN CA C 52.748 0.3 1 69 104 104 GLN CB C 27.643 0.3 1 70 104 104 GLN N N 118.769 0.3 1 71 105 105 ASP H H 8.144 0.020 1 72 105 105 ASP CA C 55.384 0.3 1 73 105 105 ASP CB C 44.494 0.3 1 74 105 105 ASP N N 119.898 0.3 1 75 106 106 GLU H H 8.108 0.020 1 76 106 106 GLU C C 175.772 0.3 1 77 106 106 GLU CA C 55.728 0.3 1 78 106 106 GLU CB C 27.528 0.3 1 79 106 106 GLU N N 119.964 0.3 1 80 107 107 GLU H H 8.471 0.020 1 81 107 107 GLU C C 176.848 0.3 1 82 107 107 GLU CA C 55.614 0.3 1 83 107 107 GLU CB C 28.445 0.3 1 84 107 107 GLU N N 124.144 0.3 1 85 108 108 GLU H H 8.394 0.020 1 86 108 108 GLU C C 174.500 0.3 1 87 108 108 GLU CA C 52.518 0.3 1 88 108 108 GLU CB C 37.043 0.3 1 89 108 108 GLU N N 118.917 0.3 1 90 109 109 ALA H H 7.989 0.020 1 91 109 109 ALA CA C 52.862 0.3 1 92 109 109 ALA CB C 17.669 0.3 1 93 109 109 ALA N N 121.563 0.3 1 94 110 110 ASP H H 8.190 0.020 1 95 110 110 ASP C C 174.965 0.3 1 96 110 110 ASP CA C 52.633 0.3 1 97 110 110 ASP CB C 37.043 0.3 1 98 110 110 ASP N N 116.800 0.3 1 99 111 111 GLU H H 8.174 0.020 1 100 111 111 GLU C C 175.674 0.3 1 101 111 111 GLU CA C 52.633 0.3 1 102 111 111 GLU CB C 31.884 0.3 1 103 111 111 GLU N N 121.321 0.3 1 104 112 112 GLU H H 8.405 0.020 1 105 112 112 GLU C C 173.522 0.3 1 106 112 112 GLU CA C 61.804 0.3 1 107 112 112 GLU CB C 27.184 0.3 1 108 112 112 GLU N N 121.491 0.3 1 109 113 113 GLU H H 8.506 0.020 1 110 113 113 GLU CA C 54.467 0.3 1 111 113 113 GLU CB C 28.216 0.3 1 112 113 113 GLU N N 119.245 0.3 1 113 114 114 GLY H H 8.237 0.020 1 114 114 114 GLY C C 173.619 0.3 1 115 114 114 GLY CA C 42.838 0.3 1 116 114 114 GLY N N 109.153 0.3 1 117 115 115 VAL H H 8.213 0.020 1 118 115 115 VAL CA C 54.545 0.3 1 119 115 115 VAL CB C 41.572 0.3 1 120 115 115 VAL N N 122.184 0.3 1 121 116 116 ARG H H 8.244 0.020 1 122 116 116 ARG CA C 55.843 0.3 1 123 116 116 ARG CB C 27.528 0.3 1 124 116 116 ARG N N 122.901 0.3 1 125 117 117 ASP H H 7.950 0.020 1 126 117 117 ASP CA C 55.384 0.3 1 127 117 117 ASP CB C 41.399 0.3 1 128 117 117 ASP N N 122.025 0.3 1 129 118 118 SER H H 8.194 0.020 1 130 118 118 SER CA C 58.594 0.3 1 131 118 118 SER CB C 62.606 0.3 1 132 118 118 SER N N 116.270 0.3 1 133 119 119 GLU H H 8.225 0.020 1 134 119 119 GLU C C 175.821 0.3 1 135 119 119 GLU CA C 55.957 0.3 1 136 119 119 GLU CB C 27.528 0.3 1 137 119 119 GLU N N 121.417 0.3 1 138 120 120 GLU H H 8.163 0.020 1 139 120 120 GLU C C 173.571 0.3 1 140 120 120 GLU CA C 54.467 0.3 1 141 120 120 GLU CB C 27.757 0.3 1 142 120 120 GLU N N 117.513 0.3 1 143 121 121 VAL H H 7.909 0.020 1 144 121 121 VAL N N 121.097 0.3 1 145 122 122 GLU H H 8.105 0.020 1 146 122 122 GLU N N 120.104 0.3 1 147 123 123 GLU H H 8.303 0.020 1 148 123 123 GLU CA C 53.467 0.3 1 149 123 123 GLU CB C 27.987 0.3 1 150 123 123 GLU N N 118.420 0.3 1 151 124 124 SER H H 8.360 0.020 1 152 124 124 SER C C 173.620 0.3 1 153 124 124 SER CA C 57.677 0.3 1 154 124 124 SER CB C 62.950 0.3 1 155 124 124 SER N N 116.889 0.3 1 156 125 125 THR H H 8.104 0.020 1 157 125 125 THR CA C 62.836 0.3 1 158 125 125 THR CB C 68.338 0.3 1 159 125 125 THR N N 115.863 0.3 1 160 127 127 LYS H H 8.189 0.020 1 161 127 127 LYS CA C 52.633 0.3 1 162 127 127 LYS CB C 37.960 0.3 1 163 127 127 LYS N N 119.839 0.3 1 164 128 128 LEU H H 8.291 0.020 1 165 128 128 LEU C C 175.381 0.3 1 166 128 128 LEU CA C 52.748 0.3 1 167 128 128 LEU CB C 37.960 0.3 1 168 128 128 LEU N N 120.346 0.3 1 169 129 129 ALA H H 8.014 0.020 1 170 129 129 ALA CA C 52.823 0.3 1 171 129 129 ALA CB C 17.576 0.3 1 172 129 129 ALA N N 123.082 0.3 1 173 130 130 ALA H H 7.994 0.020 1 174 130 130 ALA CA C 62.836 0.3 1 175 130 130 ALA CB C 17.669 0.3 1 176 130 130 ALA N N 120.818 0.3 1 177 131 131 ALA H H 7.872 0.020 1 178 131 131 ALA CA C 52.748 0.3 1 179 131 131 ALA CB C 17.669 0.3 1 180 131 131 ALA N N 123.131 0.3 1 181 132 132 LEU H H 7.816 0.020 1 182 132 132 LEU C C 175.870 0.3 1 183 132 132 LEU CA C 55.040 0.3 1 184 132 132 LEU CB C 41.284 0.3 1 185 132 132 LEU N N 119.453 0.3 1 186 133 133 GLU H H 8.596 0.020 1 187 133 133 GLU CA C 54.571 0.3 1 188 133 133 GLU CB C 28.254 0.3 1 189 133 133 GLU N N 120.112 0.3 1 190 134 134 HIS H H 8.283 0.020 1 191 134 134 HIS C C 174.647 0.3 1 192 134 134 HIS CA C 55.384 0.3 1 193 134 134 HIS CB C 27.757 0.3 1 194 134 134 HIS N N 118.829 0.3 1 195 135 135 HIS H H 8.285 0.020 1 196 135 135 HIS CA C 55.614 0.3 1 197 135 135 HIS CB C 29.706 0.3 1 198 135 135 HIS N N 123.943 0.3 1 199 136 136 HIS H H 8.044 0.020 1 200 136 136 HIS CA C 61.345 0.3 1 201 136 136 HIS CB C 31.884 0.3 1 202 136 136 HIS N N 121.685 0.3 1 203 137 137 HIS H H 8.210 0.020 1 204 137 137 HIS CA C 55.957 0.3 1 205 137 137 HIS CB C 27.528 0.3 1 206 137 137 HIS N N 121.641 0.3 1 207 138 138 HIS H H 8.533 0.020 1 208 138 138 HIS CA C 54.696 0.3 1 209 138 138 HIS CB C 28.445 0.3 1 210 138 138 HIS N N 120.701 0.3 1 211 139 139 HIS H H 8.354 0.020 1 212 139 139 HIS C C 174.745 0.3 1 213 139 139 HIS CA C 52.404 0.3 1 214 139 139 HIS CB C 37.272 0.3 1 stop_ save_