data_26684 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Chica 410-478 ; _BMRB_accession_number 26684 _BMRB_flat_file_name bmr26684.str _Entry_type original _Submission_date 2015-10-07 _Accession_date 2015-10-07 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Chemical shifts for the LC8-binding region of Chica, residues 410-478.' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Nyarko Afua . . 2 Clark Sarah A. . 3 Barbar Elisar . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 67 "13C chemical shifts" 199 "15N chemical shifts" 67 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2016-07-08 update BMRB 'update entry citation' 2015-12-17 original author 'original release' stop_ _Original_release_date 2015-12-17 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; The anchored flexibility model in LC8 motif recognition: Insights from the Chica complex ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 26652654 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Clark Sarah . . 2 Nyarko Afua . . 3 Lohr Frank . . 4 Karplus 'P. Andrew' . . 5 Barbar Elisar . . stop_ _Journal_abbreviation Biochemistry _Journal_volume 55 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 199 _Page_last 209 _Year 2015 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Chica _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Chica $Chica stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Chica _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Chica _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'regulates spindle orientation during mitosis' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 615 _Mol_residue_sequence ; MARACLIQRLPIKRDCTPVF VRGLSSPSAAMALLSEGLDE VPAACLSPCGPPNPTELFSE SRRLALEELVAGGPEAFAAF LRRERLARFLNPDEVHAILR AAERPGEEGAAAAAAAEDSF GSSHDCSSGTYFPEQSDLEP PLLELGWPAFYQGAYRGATR VETHFQPRGAGEGGPYGCKD ALRQQLRSAREVIAVVMDVF TDIDIFRDLQEICRKQGVAV YILLDQALLSQFLDMCMDLK VHPEQEKLMTVRTITGNIYY ARSGTKIIGKVHEKFTLIDG IRVATGSYSFTWTDGKLNSS NLVILSGQVVEHFDLEFRIL YAQSKPISPKLLSHFQSSNK FDHLTNRKPQSKELTLGNLL RMRLARLSSTPRKADLDPEM PAEGKAERKPHDCESSTVSE EDYFSSHRDELQSRKAIDAA TQTEPGEEMPGLSVSEVGTQ TSITTACAGTQTAVITRIAS SQTTIWSRSTTTQTDMDENI LFPRGTQSTEGSPVSKMSVS RSSSLKSSSSVSSQGSVASS TGSPASIRTTDFHNPGYPKY LGTPHLELYLSDSLRNLNKE RQFHFAGIRSRLNHMLAMLS RRTLFTENHLGLHSGNFSRV NLLAVRDVALYPSYQ ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ALA 3 ARG 4 ALA 5 CYS 6 LEU 7 ILE 8 GLN 9 ARG 10 LEU 11 PRO 12 ILE 13 LYS 14 ARG 15 ASP 16 CYS 17 THR 18 PRO 19 VAL 20 PHE 21 VAL 22 ARG 23 GLY 24 LEU 25 SER 26 SER 27 PRO 28 SER 29 ALA 30 ALA 31 MET 32 ALA 33 LEU 34 LEU 35 SER 36 GLU 37 GLY 38 LEU 39 ASP 40 GLU 41 VAL 42 PRO 43 ALA 44 ALA 45 CYS 46 LEU 47 SER 48 PRO 49 CYS 50 GLY 51 PRO 52 PRO 53 ASN 54 PRO 55 THR 56 GLU 57 LEU 58 PHE 59 SER 60 GLU 61 SER 62 ARG 63 ARG 64 LEU 65 ALA 66 LEU 67 GLU 68 GLU 69 LEU 70 VAL 71 ALA 72 GLY 73 GLY 74 PRO 75 GLU 76 ALA 77 PHE 78 ALA 79 ALA 80 PHE 81 LEU 82 ARG 83 ARG 84 GLU 85 ARG 86 LEU 87 ALA 88 ARG 89 PHE 90 LEU 91 ASN 92 PRO 93 ASP 94 GLU 95 VAL 96 HIS 97 ALA 98 ILE 99 LEU 100 ARG 101 ALA 102 ALA 103 GLU 104 ARG 105 PRO 106 GLY 107 GLU 108 GLU 109 GLY 110 ALA 111 ALA 112 ALA 113 ALA 114 ALA 115 ALA 116 ALA 117 GLU 118 ASP 119 SER 120 PHE 121 GLY 122 SER 123 SER 124 HIS 125 ASP 126 CYS 127 SER 128 SER 129 GLY 130 THR 131 TYR 132 PHE 133 PRO 134 GLU 135 GLN 136 SER 137 ASP 138 LEU 139 GLU 140 PRO 141 PRO 142 LEU 143 LEU 144 GLU 145 LEU 146 GLY 147 TRP 148 PRO 149 ALA 150 PHE 151 TYR 152 GLN 153 GLY 154 ALA 155 TYR 156 ARG 157 GLY 158 ALA 159 THR 160 ARG 161 VAL 162 GLU 163 THR 164 HIS 165 PHE 166 GLN 167 PRO 168 ARG 169 GLY 170 ALA 171 GLY 172 GLU 173 GLY 174 GLY 175 PRO 176 TYR 177 GLY 178 CYS 179 LYS 180 ASP 181 ALA 182 LEU 183 ARG 184 GLN 185 GLN 186 LEU 187 ARG 188 SER 189 ALA 190 ARG 191 GLU 192 VAL 193 ILE 194 ALA 195 VAL 196 VAL 197 MET 198 ASP 199 VAL 200 PHE 201 THR 202 ASP 203 ILE 204 ASP 205 ILE 206 PHE 207 ARG 208 ASP 209 LEU 210 GLN 211 GLU 212 ILE 213 CYS 214 ARG 215 LYS 216 GLN 217 GLY 218 VAL 219 ALA 220 VAL 221 TYR 222 ILE 223 LEU 224 LEU 225 ASP 226 GLN 227 ALA 228 LEU 229 LEU 230 SER 231 GLN 232 PHE 233 LEU 234 ASP 235 MET 236 CYS 237 MET 238 ASP 239 LEU 240 LYS 241 VAL 242 HIS 243 PRO 244 GLU 245 GLN 246 GLU 247 LYS 248 LEU 249 MET 250 THR 251 VAL 252 ARG 253 THR 254 ILE 255 THR 256 GLY 257 ASN 258 ILE 259 TYR 260 TYR 261 ALA 262 ARG 263 SER 264 GLY 265 THR 266 LYS 267 ILE 268 ILE 269 GLY 270 LYS 271 VAL 272 HIS 273 GLU 274 LYS 275 PHE 276 THR 277 LEU 278 ILE 279 ASP 280 GLY 281 ILE 282 ARG 283 VAL 284 ALA 285 THR 286 GLY 287 SER 288 TYR 289 SER 290 PHE 291 THR 292 TRP 293 THR 294 ASP 295 GLY 296 LYS 297 LEU 298 ASN 299 SER 300 SER 301 ASN 302 LEU 303 VAL 304 ILE 305 LEU 306 SER 307 GLY 308 GLN 309 VAL 310 VAL 311 GLU 312 HIS 313 PHE 314 ASP 315 LEU 316 GLU 317 PHE 318 ARG 319 ILE 320 LEU 321 TYR 322 ALA 323 GLN 324 SER 325 LYS 326 PRO 327 ILE 328 SER 329 PRO 330 LYS 331 LEU 332 LEU 333 SER 334 HIS 335 PHE 336 GLN 337 SER 338 SER 339 ASN 340 LYS 341 PHE 342 ASP 343 HIS 344 LEU 345 THR 346 ASN 347 ARG 348 LYS 349 PRO 350 GLN 351 SER 352 LYS 353 GLU 354 LEU 355 THR 356 LEU 357 GLY 358 ASN 359 LEU 360 LEU 361 ARG 362 MET 363 ARG 364 LEU 365 ALA 366 ARG 367 LEU 368 SER 369 SER 370 THR 371 PRO 372 ARG 373 LYS 374 ALA 375 ASP 376 LEU 377 ASP 378 PRO 379 GLU 380 MET 381 PRO 382 ALA 383 GLU 384 GLY 385 LYS 386 ALA 387 GLU 388 ARG 389 LYS 390 PRO 391 HIS 392 ASP 393 CYS 394 GLU 395 SER 396 SER 397 THR 398 VAL 399 SER 400 GLU 401 GLU 402 ASP 403 TYR 404 PHE 405 SER 406 SER 407 HIS 408 ARG 409 ASP 410 GLU 411 LEU 412 GLN 413 SER 414 ARG 415 LYS 416 ALA 417 ILE 418 ASP 419 ALA 420 ALA 421 THR 422 GLN 423 THR 424 GLU 425 PRO 426 GLY 427 GLU 428 GLU 429 MET 430 PRO 431 GLY 432 LEU 433 SER 434 VAL 435 SER 436 GLU 437 VAL 438 GLY 439 THR 440 GLN 441 THR 442 SER 443 ILE 444 THR 445 THR 446 ALA 447 CYS 448 ALA 449 GLY 450 THR 451 GLN 452 THR 453 ALA 454 VAL 455 ILE 456 THR 457 ARG 458 ILE 459 ALA 460 SER 461 SER 462 GLN 463 THR 464 THR 465 ILE 466 TRP 467 SER 468 ARG 469 SER 470 THR 471 THR 472 THR 473 GLN 474 THR 475 ASP 476 MET 477 ASP 478 GLU 479 ASN 480 ILE 481 LEU 482 PHE 483 PRO 484 ARG 485 GLY 486 THR 487 GLN 488 SER 489 THR 490 GLU 491 GLY 492 SER 493 PRO 494 VAL 495 SER 496 LYS 497 MET 498 SER 499 VAL 500 SER 501 ARG 502 SER 503 SER 504 SER 505 LEU 506 LYS 507 SER 508 SER 509 SER 510 SER 511 VAL 512 SER 513 SER 514 GLN 515 GLY 516 SER 517 VAL 518 ALA 519 SER 520 SER 521 THR 522 GLY 523 SER 524 PRO 525 ALA 526 SER 527 ILE 528 ARG 529 THR 530 THR 531 ASP 532 PHE 533 HIS 534 ASN 535 PRO 536 GLY 537 TYR 538 PRO 539 LYS 540 TYR 541 LEU 542 GLY 543 THR 544 PRO 545 HIS 546 LEU 547 GLU 548 LEU 549 TYR 550 LEU 551 SER 552 ASP 553 SER 554 LEU 555 ARG 556 ASN 557 LEU 558 ASN 559 LYS 560 GLU 561 ARG 562 GLN 563 PHE 564 HIS 565 PHE 566 ALA 567 GLY 568 ILE 569 ARG 570 SER 571 ARG 572 LEU 573 ASN 574 HIS 575 MET 576 LEU 577 ALA 578 MET 579 LEU 580 SER 581 ARG 582 ARG 583 THR 584 LEU 585 PHE 586 THR 587 GLU 588 ASN 589 HIS 590 LEU 591 GLY 592 LEU 593 HIS 594 SER 595 GLY 596 ASN 597 PHE 598 SER 599 ARG 600 VAL 601 ASN 602 LEU 603 LEU 604 ALA 605 VAL 606 ARG 607 ASP 608 VAL 609 ALA 610 LEU 611 TYR 612 PRO 613 SER 614 TYR 615 GLN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Chica Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Chica 'recombinant technology' . Escherichia coli BL21-DE3 pET15 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Chica 250 uM '[U-99% 13C; U-99% 15N]' DSS 1 mM 'natural abundance' 'sodium azide' 1 mM 'natural abundance' 'sodium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' arginine/glutamate 50 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version 3.5 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address 'Shen and Bax' . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(COCA)CB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(COCA)CB' _Sample_label $sample_1 save_ save_3D_HNCO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.01 . M pH 7.0 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0 internal direct . . . 0.25 DSS H 1 'methyl protons' ppm 0 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0 internal direct . . . 0.1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D HN(COCA)CB' '3D HNCO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Chica _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 410 410 GLU H H 8.515 . 1 2 410 410 GLU C C 176.997 . 1 3 410 410 GLU CA C 57.179 . 1 4 410 410 GLU CB C 29.884 . 1 5 410 410 GLU N N 122.845 . 1 6 411 411 LEU H H 8.203 . 1 7 411 411 LEU C C 176.127 . 1 8 411 411 LEU CA C 55.872 . 1 9 411 411 LEU CB C 42.149 . 1 10 411 411 LEU N N 122.258 . 1 11 412 412 GLN H H 8.313 . 1 12 412 412 GLN C C 176.478 . 1 13 412 412 GLN CA C 55.398 . 1 14 412 412 GLN CB C 33.108 . 1 15 412 412 GLN N N 120.02 . 1 16 413 413 SER H H 8.283 . 1 17 413 413 SER C C 174.777 . 1 18 413 413 SER CA C 58.749 . 1 19 413 413 SER CB C 63.626 . 1 20 413 413 SER N N 116.527 . 1 21 414 414 ARG H H 8.304 . 1 22 414 414 ARG C C 176.426 . 1 23 414 414 ARG CA C 56.38 . 1 24 414 414 ARG CB C 30.715 . 1 25 414 414 ARG N N 122.998 . 1 26 415 415 LYS H H 8.304 . 1 27 415 415 LYS C C 176.622 . 1 28 415 415 LYS CA C 56.506 . 1 29 415 415 LYS CB C 33.106 . 1 30 415 415 LYS N N 122.439 . 1 31 416 416 ALA H H 8.347 . 1 32 416 416 ALA C C 178.154 . 1 33 416 416 ALA CA C 52.764 . 1 34 416 416 ALA CB C 19.102 . 1 35 416 416 ALA N N 125.408 . 1 36 417 417 ILE H H 8.154 . 1 37 417 417 ILE C C 176.369 . 1 38 417 417 ILE CA C 61.564 . 1 39 417 417 ILE CB C 38.846 . 1 40 417 417 ILE N N 120.004 . 1 41 418 418 ASP H H 8.37 . 1 42 418 418 ASP C C 176.256 . 1 43 418 418 ASP CA C 54.4 . 1 44 418 418 ASP CB C 41.079 . 1 45 418 418 ASP N N 123.924 . 1 46 419 419 ALA H H 8.238 . 1 47 419 419 ALA C C 177.884 . 1 48 419 419 ALA CA C 52.974 . 1 49 419 419 ALA CB C 19.149 . 1 50 419 419 ALA N N 124.651 . 1 51 420 420 ALA H H 8.296 . 1 52 420 420 ALA C C 178.28 . 1 53 420 420 ALA CA C 52.883 . 1 54 420 420 ALA CB C 19.12 . 1 55 420 420 ALA N N 122.327 . 1 56 421 421 THR H H 8.038 . 1 57 421 421 THR C C 174.462 . 1 58 421 421 THR CA C 62.036 . 1 59 421 421 THR CB C 69.794 . 1 60 421 421 THR N N 112.806 . 1 61 422 422 GLN H H 8.528 . 1 62 422 422 GLN C C 176.117 . 1 63 422 422 GLN CA C 56.07 . 1 64 422 422 GLN CB C 29.611 . 1 65 422 422 GLN N N 123.39 . 1 66 423 423 THR H H 8.265 . 1 67 423 423 THR C C 174.383 . 1 68 423 423 THR CA C 61.886 . 1 69 423 423 THR CB C 69.799 . 1 70 423 423 THR N N 116.281 . 1 71 424 424 GLU H H 8.553 . 1 72 424 424 GLU CA C 54.255 . 1 73 424 424 GLU CB C 29.669 . 1 74 424 424 GLU N N 124.91 . 1 75 425 425 PRO C C 177.609 . 1 76 425 425 PRO CA C 63.579 . 1 77 425 425 PRO CB C 32.101 . 1 78 426 426 GLY H H 8.576 . 1 79 426 426 GLY C C 174.118 . 1 80 426 426 GLY CA C 45.088 . 1 81 426 426 GLY N N 109.747 . 1 82 427 427 GLU H H 8.241 . 1 83 427 427 GLU C C 176.439 . 1 84 427 427 GLU CA C 56.258 . 1 85 427 427 GLU CB C 30.589 . 1 86 427 427 GLU N N 120.521 . 1 87 428 428 GLU H H 8.605 . 1 88 428 428 GLU C C 176.324 . 1 89 428 428 GLU CA C 56.427 . 1 90 428 428 GLU CB C 30.37 . 1 91 428 428 GLU N N 122.387 . 1 92 429 429 MET H H 8.547 . 1 93 429 429 MET CA C 53.199 . 1 94 429 429 MET CB C 32.373 . 1 95 429 429 MET N N 123.279 . 1 96 430 430 PRO C C 177.586 . 1 97 430 430 PRO CA C 63.424 . 1 98 430 430 PRO CB C 32.065 . 1 99 431 431 GLY H H 8.549 . 1 100 431 431 GLY C C 174.128 . 1 101 431 431 GLY CA C 45.241 . 1 102 431 431 GLY N N 109.294 . 1 103 432 432 LEU H H 8.137 . 1 104 432 432 LEU C C 177.59 . 1 105 432 432 LEU CA C 55.152 . 1 106 432 432 LEU CB C 42.662 . 1 107 432 432 LEU N N 121.74 . 1 108 433 433 SER H H 8.467 . 1 109 433 433 SER C C 174.682 . 1 110 433 433 SER CA C 58.153 . 1 111 433 433 SER CB C 63.793 . 1 112 433 433 SER N N 117.396 . 1 113 434 434 VAL H H 8.298 . 1 114 434 434 VAL C C 176.367 . 1 115 434 434 VAL CA C 62.376 . 1 116 434 434 VAL CB C 32.877 . 1 117 434 434 VAL N N 121.876 . 1 118 435 435 SER H H 8.407 . 1 119 435 435 SER C C 174.605 . 1 120 435 435 SER CA C 58.429 . 1 121 435 435 SER CB C 63.789 . 1 122 435 435 SER N N 119.105 . 1 123 436 436 GLU H H 8.494 . 1 124 436 436 GLU C C 176.669 . 1 125 436 436 GLU CA C 56.547 . 1 126 436 436 GLU CB C 30.363 . 1 127 436 436 GLU N N 123.391 . 1 128 437 437 VAL H H 8.245 . 1 129 437 437 VAL C C 176.934 . 1 130 437 437 VAL CA C 62.818 . 1 131 437 437 VAL CB C 32.599 . 1 132 437 437 VAL N N 121.088 . 1 133 438 438 GLY H H 8.542 . 1 134 438 438 GLY C C 174.495 . 1 135 438 438 GLY CA C 45.335 . 1 136 438 438 GLY N N 112.32 . 1 137 439 439 THR H H 8.103 . 1 138 439 439 THR C C 174.85 . 1 139 439 439 THR CA C 62.012 . 1 140 439 439 THR CB C 69.853 . 1 141 439 439 THR N N 113.73 . 1 142 440 440 GLN H H 8.584 . 1 143 440 440 GLN C C 176.223 . 1 144 440 440 GLN CA C 55.928 . 1 145 440 440 GLN CB C 29.426 . 1 146 440 440 GLN N N 123.073 . 1 147 441 441 THR H H 8.321 . 1 148 441 441 THR C C 174.568 . 1 149 441 441 THR CA C 61.962 . 1 150 441 441 THR CB C 69.816 . 1 151 441 441 THR N N 115.871 . 1 152 442 442 SER H H 8.42 . 1 153 442 442 SER C C 174.479 . 1 154 442 442 SER CA C 58.252 . 1 155 442 442 SER CB C 63.821 . 1 156 442 442 SER N N 118.584 . 1 157 443 443 ILE H H 8.306 . 1 158 443 443 ILE C C 176.701 . 1 159 443 443 ILE CA C 61.38 . 1 160 443 443 ILE CB C 38.79 . 1 161 443 443 ILE N N 122.884 . 1 162 444 444 THR H H 8.327 . 1 163 444 444 THR C C 174.794 . 1 164 444 444 THR CA C 61.833 . 1 165 444 444 THR CB C 69.785 . 1 166 444 444 THR N N 118.384 . 1 167 445 445 THR H H 8.197 . 1 168 445 445 THR C C 174.361 . 1 169 445 445 THR CA C 61.974 . 1 170 445 445 THR CB C 69.777 . 1 171 445 445 THR N N 116.912 . 1 172 446 446 ALA H H 8.386 . 1 173 446 446 ALA C C 177.69 . 1 174 446 446 ALA CA C 52.607 . 1 175 446 446 ALA CB C 19.264 . 1 176 446 446 ALA N N 126.695 . 1 177 447 447 CYS H H 8.389 . 1 178 447 447 CYS C C 174.524 . 1 179 447 447 CYS CA C 58.28 . 1 180 447 447 CYS CB C 28.07 . 1 181 447 447 CYS N N 118.969 . 1 182 448 448 ALA H H 8.527 . 1 183 448 448 ALA C C 178.286 . 1 184 448 448 ALA CA C 52.979 . 1 185 448 448 ALA CB C 19.28 . 1 186 448 448 ALA N N 127.015 . 1 187 449 449 GLY H H 8.465 . 1 188 449 449 GLY C C 174.475 . 1 189 449 449 GLY CA C 45.321 . 1 190 449 449 GLY N N 108.396 . 1 191 450 450 THR H H 8.117 . 1 192 450 450 THR C C 174.57 . 1 193 450 450 THR CA C 62.012 . 1 194 450 450 THR CB C 69.853 . 1 195 450 450 THR N N 113.766 . 1 196 451 451 GLN H H 8.367 . 1 197 451 451 GLN C C 175.898 . 1 198 451 451 GLN CA C 55.702 . 1 199 451 451 GLN CB C 29.617 . 1 200 451 451 GLN N N 122.949 . 1 201 452 452 THR H H 8.28 . 1 202 452 452 THR C C 174.193 . 1 203 452 452 THR CA C 61.892 . 1 204 452 452 THR CB C 69.972 . 1 205 452 452 THR N N 116.22 . 1 206 453 453 ALA H H 8.39 . 1 207 453 453 ALA C C 177.556 . 1 208 453 453 ALA CA C 52.509 . 1 209 453 453 ALA CB C 19.254 . 1 210 453 453 ALA N N 127.058 . 1 211 454 454 VAL H H 8.229 . 1 212 454 454 VAL C C 176.306 . 1 213 454 454 VAL CA C 62.581 . 1 214 454 454 VAL CB C 32.663 . 1 215 454 454 VAL N N 120.662 . 1 216 455 455 ILE H H 8.369 . 1 217 455 455 ILE C C 176.454 . 1 218 455 455 ILE CA C 60.946 . 1 219 455 455 ILE CB C 38.575 . 1 220 455 455 ILE N N 125.837 . 1 221 456 456 THR H H 8.345 . 1 222 456 456 THR C C 174.219 . 1 223 456 456 THR CA C 62.041 . 1 224 456 456 THR CB C 69.768 . 1 225 456 456 THR N N 119.991 . 1 226 457 457 ARG H H 8.424 . 1 227 457 457 ARG C C 176.076 . 1 228 457 457 ARG CA C 55.979 . 1 229 457 457 ARG CB C 30.889 . 1 230 457 457 ARG N N 124.529 . 1 231 458 458 ILE H H 8.309 . 1 232 458 458 ILE C C 176.155 . 1 233 458 458 ILE CA C 61.013 . 1 234 458 458 ILE CB C 38.722 . 1 235 458 458 ILE N N 123.158 . 1 236 459 459 ALA H H 8.531 . 1 237 459 459 ALA C C 177.85 . 1 238 459 459 ALA CA C 52.705 . 1 239 459 459 ALA CB C 19.202 . 1 240 459 459 ALA N N 128.914 . 1 241 460 460 SER H H 8.391 . 1 242 460 460 SER C C 174.864 . 1 243 460 460 SER CA C 58.38 . 1 244 460 460 SER CB C 63.808 . 1 245 460 460 SER N N 115.522 . 1 246 461 461 SER H H 8.427 . 1 247 461 461 SER C C 174.672 . 1 248 461 461 SER CA C 58.634 . 1 249 461 461 SER CB C 63.719 . 1 250 461 461 SER N N 117.773 . 1 251 462 462 GLN H H 8.453 . 1 252 462 462 GLN C C 176.206 . 1 253 462 462 GLN CA C 56.013 . 1 254 462 462 GLN CB C 29.403 . 1 255 462 462 GLN N N 122.026 . 1 256 463 463 THR H H 8.245 . 1 257 463 463 THR C C 174.697 . 1 258 463 463 THR CA C 62.141 . 1 259 463 463 THR CB C 69.766 . 1 260 463 463 THR N N 115.45 . 1 261 464 464 THR H H 8.217 . 1 262 464 464 THR C C 174.45 . 1 263 464 464 THR CA C 62.006 . 1 264 464 464 THR CB C 69.966 . 1 265 464 464 THR N N 117.383 . 1 266 465 465 ILE H H 8.193 . 1 267 465 465 ILE CA C 61.509 . 1 268 465 465 ILE CB C 38.527 . 1 269 465 465 ILE N N 123.182 . 1 270 466 466 TRP H H 10.158 . 1 271 466 466 TRP C C 176.284 . 1 272 466 466 TRP CA C 57.474 . 1 273 466 466 TRP CB C 29.662 . 1 274 466 466 TRP N N 129.529 . 1 275 467 467 SER H H 8.055 . 1 276 467 467 SER C C 174.095 . 1 277 467 467 SER CA C 58.072 . 1 278 467 467 SER CB C 63.867 . 1 279 467 467 SER N N 117.767 . 1 280 468 468 ARG H H 8.223 . 1 281 468 468 ARG C C 176.412 . 1 282 468 468 ARG CA C 56.261 . 1 283 468 468 ARG CB C 30.772 . 1 284 468 468 ARG N N 122.856 . 1 285 469 469 SER H H 8.414 . 1 286 469 469 SER C C 174.978 . 1 287 469 469 SER CA C 58.385 . 1 288 469 469 SER CB C 63.82 . 1 289 469 469 SER N N 116.968 . 1 290 470 470 THR H H 8.28 . 1 291 470 470 THR C C 174.898 . 1 292 470 470 THR CA C 61.878 . 1 293 470 470 THR CB C 69.855 . 1 294 470 470 THR N N 115.731 . 1 295 471 471 THR H H 8.24 . 1 296 471 471 THR C C 174.742 . 1 297 471 471 THR CA C 61.886 . 1 298 471 471 THR CB C 69.799 . 1 299 471 471 THR N N 115.947 . 1 300 472 472 THR H H 8.236 . 1 301 472 472 THR C C 174.85 . 1 302 472 472 THR CA C 61.961 . 1 303 472 472 THR CB C 69.819 . 1 304 472 472 THR N N 116.697 . 1 305 473 473 GLN H H 8.585 . 1 306 473 473 GLN C C 176.064 . 1 307 473 473 GLN CA C 55.928 . 1 308 473 473 GLN CB C 29.426 . 1 309 473 473 GLN N N 123.013 . 1 310 474 474 THR H H 8.337 . 1 311 474 474 THR C C 174.335 . 1 312 474 474 THR CA C 61.916 . 1 313 474 474 THR CB C 69.948 . 1 314 474 474 THR N N 115.517 . 1 315 475 475 ASP H H 8.464 . 1 316 475 475 ASP C C 177.97 . 1 317 475 475 ASP CA C 54.638 . 1 318 475 475 ASP CB C 41.001 . 1 319 475 475 ASP N N 122.408 . 1 320 476 476 MET H H 8.315 . 1 321 476 476 MET C C 175.938 . 1 322 476 476 MET CA C 56.185 . 1 323 476 476 MET CB C 33.108 . 1 324 476 476 MET N N 120.211 . 1 325 477 477 ASP H H 8.403 . 1 326 477 477 ASP C C 175.257 . 1 327 477 477 ASP CA C 54.646 . 1 328 477 477 ASP CB C 41.229 . 1 329 477 477 ASP N N 122.081 . 1 330 478 478 GLU H H 7.934 . 1 331 478 478 GLU CA C 58.032 . 1 332 478 478 GLU CB C 31.269 . 1 333 478 478 GLU N N 125.858 . 1 stop_ save_