data_26701 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; T-STAR KH domain ; _BMRB_accession_number 26701 _BMRB_flat_file_name bmr26701.str _Entry_type original _Submission_date 2015-11-04 _Accession_date 2015-11-04 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'KH domain of T-STAR' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Feracci Mikael . . 2 Dominguez Cyril . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 101 "15N chemical shifts" 101 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2016-12-09 update BMRB 'update entry citation' 2016-01-06 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 26700 'Sam68 STAR' stop_ _Original_release_date 2016-01-06 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68 ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 26758068 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Feracci Mikael . . 2 Foot Jaelle N. . 3 Grellscheid Sushma N. . 4 Danilenko Marina . . 5 Stehle Ralf . . 6 Gonchar Oksana . . 7 Kang Hyun-Seo . . 8 Dalgliesh Caroline . . 9 Meyer Helge N. . 10 Liu Yilei . . 11 Lahat Albert . . 12 Sattler Michael . . 13 Eperon Ian C. . 14 Elliott David J. . 15 Dominguez Cyril . . stop_ _Journal_abbreviation 'Nat. Commun.' _Journal_volume 7 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 10355 _Page_last 10355 _Year 2016 _Details . loop_ _Keyword 'KH domain' 'STAR proteins' 'alternative splicing' 'protein-RNA complexe' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'T-STAR KH' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'KH domain' $T_STAR stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_T_STAR _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common T_STAR _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 113 _Mol_residue_sequence ; GAINKNMKLGQKVLIPVKQF PKFNFVGKLLGPRGNSLKRL QEETLTKMSILGKGSMRDKA KEEELRKSGEAKYFHLNDDL HVLIEVFAPPAEAYARMGHA LEEIKKFLIPDYN ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 48 GLY 2 49 ALA 3 50 ILE 4 51 ASN 5 52 LYS 6 53 ASN 7 54 MET 8 55 LYS 9 56 LEU 10 57 GLY 11 58 GLN 12 59 LYS 13 60 VAL 14 61 LEU 15 62 ILE 16 63 PRO 17 64 VAL 18 65 LYS 19 66 GLN 20 67 PHE 21 68 PRO 22 69 LYS 23 70 PHE 24 71 ASN 25 72 PHE 26 73 VAL 27 74 GLY 28 75 LYS 29 76 LEU 30 77 LEU 31 78 GLY 32 79 PRO 33 80 ARG 34 81 GLY 35 82 ASN 36 83 SER 37 84 LEU 38 85 LYS 39 86 ARG 40 87 LEU 41 88 GLN 42 89 GLU 43 90 GLU 44 91 THR 45 92 LEU 46 93 THR 47 94 LYS 48 95 MET 49 96 SER 50 97 ILE 51 98 LEU 52 99 GLY 53 100 LYS 54 101 GLY 55 102 SER 56 103 MET 57 104 ARG 58 105 ASP 59 106 LYS 60 107 ALA 61 108 LYS 62 109 GLU 63 110 GLU 64 111 GLU 65 112 LEU 66 113 ARG 67 114 LYS 68 115 SER 69 116 GLY 70 117 GLU 71 118 ALA 72 119 LYS 73 120 TYR 74 121 PHE 75 122 HIS 76 123 LEU 77 124 ASN 78 125 ASP 79 126 ASP 80 127 LEU 81 128 HIS 82 129 VAL 83 130 LEU 84 131 ILE 85 132 GLU 86 133 VAL 87 134 PHE 88 135 ALA 89 136 PRO 90 137 PRO 91 138 ALA 92 139 GLU 93 140 ALA 94 141 TYR 95 142 ALA 96 143 ARG 97 144 MET 98 145 GLY 99 146 HIS 100 147 ALA 101 148 LEU 102 149 GLU 103 150 GLU 104 151 ILE 105 152 LYS 106 153 LYS 107 154 PHE 108 155 LEU 109 156 ILE 110 157 PRO 111 158 ASP 112 159 TYR 113 160 ASN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value UNP O75525 KHDR3_human . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Gene_mnemonic $T_STAR human 9606 Eukaryota Metazoa Homo sapiens KHDRBS3 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $T_STAR 'recombinant technology' . Escherichia coli Rosetta pLEICS-03 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $T_STAR 0.5 mM [U-15N] $T_STAR 1 mM '[U-13C; U-15N]' H2O 93 % 'natural abundance' D2O 7 % 'natural abundance' 'sodium phosphate' 20 mM 'natural abundance' NaCl 50 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version 3.110 loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 50 . mM pH 6.2 . pH pressure 1 . atm temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.7 internal direct . . . 1 water N 15 protons ppm 4.7 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $SPARKY stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCA' '3D HNCACB' '3D CBCA(CO)NH' '3D 1H-15N NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'KH domain' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 53 6 ASN H H 8.161 0.05 1 2 53 6 ASN N N 119.676 0.2 1 3 54 7 MET H H 8.476 0.05 1 4 54 7 MET N N 120.341 0.2 1 5 55 8 LYS H H 8.352 0.05 1 6 55 8 LYS N N 121.240 0.2 1 7 56 9 LEU H H 8.472 0.05 1 8 56 9 LEU N N 124.542 0.2 1 9 57 10 GLY H H 8.285 0.05 1 10 57 10 GLY N N 109.107 0.2 1 11 58 11 GLN H H 8.859 0.05 1 12 58 11 GLN N N 122.279 0.2 1 13 59 12 LYS H H 8.609 0.05 1 14 59 12 LYS N N 126.919 0.2 1 15 60 13 VAL H H 9.515 0.05 1 16 60 13 VAL N N 125.367 0.2 1 17 61 14 LEU H H 8.498 0.05 1 18 61 14 LEU N N 127.701 0.2 1 19 62 15 ILE H H 7.597 0.05 1 20 62 15 ILE N N 123.481 0.2 1 21 64 17 VAL H H 8.730 0.05 1 22 64 17 VAL N N 121.954 0.2 1 23 65 18 LYS H H 8.306 0.05 1 24 65 18 LYS N N 117.204 0.2 1 25 66 19 GLN H H 7.396 0.05 1 26 66 19 GLN N N 117.423 0.2 1 27 67 20 PHE H H 7.308 0.05 1 28 67 20 PHE N N 115.496 0.2 1 29 69 22 LYS H H 8.699 0.05 1 30 69 22 LYS N N 116.568 0.2 1 31 70 23 PHE H H 7.554 0.05 1 32 70 23 PHE N N 121.075 0.2 1 33 72 25 PHE H H 8.134 0.05 1 34 72 25 PHE N N 124.405 0.2 1 35 73 26 VAL H H 8.322 0.05 1 36 73 26 VAL N N 118.712 0.2 1 37 74 27 GLY H H 7.561 0.05 1 38 74 27 GLY N N 104.215 0.2 1 39 75 28 LYS H H 7.190 0.05 1 40 75 28 LYS N N 122.210 0.2 1 41 76 29 LEU H H 8.112 0.05 1 42 76 29 LEU N N 118.541 0.2 1 43 77 30 LEU H H 7.980 0.05 1 44 77 30 LEU N N 113.462 0.2 1 45 78 31 GLY H H 7.656 0.05 1 46 78 31 GLY N N 105.358 0.2 1 47 80 33 ARG H H 8.306 0.05 1 48 80 33 ARG N N 120.326 0.2 1 49 81 34 GLY H H 7.734 0.05 1 50 81 34 GLY N N 106.597 0.2 1 51 82 35 ASN H H 8.223 0.05 1 52 82 35 ASN N N 117.577 0.2 1 53 83 36 SER H H 8.744 0.05 1 54 83 36 SER N N 118.129 0.2 1 55 84 37 LEU H H 7.453 0.05 1 56 84 37 LEU N N 124.445 0.2 1 57 85 38 LYS H H 7.796 0.05 1 58 85 38 LYS N N 120.999 0.2 1 59 86 39 ARG H H 7.869 0.05 1 60 86 39 ARG N N 119.074 0.2 1 61 87 40 LEU H H 7.974 0.05 1 62 87 40 LEU N N 119.949 0.2 1 63 88 41 GLN H H 8.031 0.05 1 64 88 41 GLN N N 120.547 0.2 1 65 89 42 GLU H H 7.906 0.05 1 66 89 42 GLU N N 120.045 0.2 1 67 90 43 GLU H H 8.590 0.05 1 68 90 43 GLU N N 116.829 0.2 1 69 91 44 THR H H 7.527 0.05 1 70 91 44 THR N N 104.234 0.2 1 71 92 45 LEU H H 7.894 0.05 1 72 92 45 LEU N N 119.190 0.2 1 73 93 46 THR H H 7.783 0.05 1 74 93 46 THR N N 106.827 0.2 1 75 94 47 LYS H H 8.642 0.05 1 76 94 47 LYS N N 119.091 0.2 1 77 95 48 MET H H 8.398 0.05 1 78 95 48 MET N N 122.641 0.2 1 79 96 49 SER H H 8.179 0.05 1 80 96 49 SER N N 114.412 0.2 1 81 97 50 ILE H H 8.594 0.05 1 82 97 50 ILE N N 124.909 0.2 1 83 98 51 LEU H H 8.447 0.05 1 84 98 51 LEU N N 129.526 0.2 1 85 99 52 GLY H H 10.006 0.05 1 86 99 52 GLY N N 106.940 0.2 1 87 100 53 LYS H H 8.778 0.05 1 88 100 53 LYS N N 121.461 0.2 1 89 101 54 GLY H H 11.040 0.05 1 90 101 54 GLY N N 117.119 0.2 1 91 102 55 SER H H 8.335 0.05 1 92 102 55 SER N N 115.364 0.2 1 93 103 56 MET H H 8.300 0.05 1 94 103 56 MET N N 119.334 0.2 1 95 104 57 ARG H H 7.944 0.05 1 96 104 57 ARG N N 117.595 0.2 1 97 105 58 ASP H H 7.770 0.05 1 98 105 58 ASP N N 117.141 0.2 1 99 106 59 LYS H H 8.508 0.05 1 100 106 59 LYS N N 126.244 0.2 1 101 107 60 ALA H H 8.136 0.05 1 102 107 60 ALA N N 122.075 0.2 1 103 108 61 LYS H H 7.727 0.05 1 104 108 61 LYS N N 120.914 0.2 1 105 109 62 GLU H H 8.168 0.05 1 106 109 62 GLU N N 118.441 0.2 1 107 110 63 GLU H H 7.729 0.05 1 108 110 63 GLU N N 117.279 0.2 1 109 111 64 GLU H H 7.524 0.05 1 110 111 64 GLU N N 119.006 0.2 1 111 112 65 LEU H H 8.163 0.05 1 112 112 65 LEU N N 119.412 0.2 1 113 113 66 ARG H H 8.621 0.05 1 114 113 66 ARG N N 122.515 0.2 1 115 114 67 LYS H H 7.883 0.05 1 116 114 67 LYS N N 117.232 0.2 1 117 115 68 SER H H 7.608 0.05 1 118 115 68 SER N N 114.676 0.2 1 119 116 69 GLY H H 7.650 0.05 1 120 116 69 GLY N N 109.029 0.2 1 121 117 70 GLU H H 7.861 0.05 1 122 117 70 GLU N N 120.058 0.2 1 123 118 71 ALA H H 8.623 0.05 1 124 118 71 ALA N N 130.195 0.2 1 125 119 72 LYS H H 8.235 0.05 1 126 119 72 LYS N N 116.297 0.2 1 127 120 73 TYR H H 7.348 0.05 1 128 120 73 TYR N N 115.320 0.2 1 129 121 74 PHE H H 7.765 0.05 1 130 121 74 PHE N N 122.014 0.2 1 131 122 75 HIS H H 8.306 0.05 1 132 122 75 HIS N N 117.568 0.2 1 133 123 76 LEU H H 7.210 0.05 1 134 123 76 LEU N N 119.125 0.2 1 135 124 77 ASN H H 7.724 0.05 1 136 124 77 ASN N N 114.587 0.2 1 137 125 78 ASP H H 8.137 0.05 1 138 125 78 ASP N N 122.232 0.2 1 139 126 79 ASP H H 8.610 0.05 1 140 126 79 ASP N N 119.283 0.2 1 141 127 80 LEU H H 8.391 0.05 1 142 127 80 LEU N N 124.482 0.2 1 143 128 81 HIS H H 9.176 0.05 1 144 128 81 HIS N N 122.918 0.2 1 145 129 82 VAL H H 8.794 0.05 1 146 129 82 VAL N N 117.287 0.2 1 147 130 83 LEU H H 9.068 0.05 1 148 130 83 LEU N N 129.209 0.2 1 149 131 84 ILE H H 8.799 0.05 1 150 131 84 ILE N N 129.659 0.2 1 151 132 85 GLU H H 9.409 0.05 1 152 132 85 GLU N N 124.980 0.2 1 153 133 86 VAL H H 8.694 0.05 1 154 133 86 VAL N N 119.851 0.2 1 155 134 87 PHE H H 8.239 0.05 1 156 134 87 PHE N N 127.184 0.2 1 157 135 88 ALA H H 8.545 0.05 1 158 135 88 ALA N N 127.318 0.2 1 159 138 91 ALA H H 8.111 0.05 1 160 138 91 ALA N N 122.505 0.2 1 161 139 92 GLU H H 8.021 0.05 1 162 139 92 GLU N N 119.755 0.2 1 163 140 93 ALA H H 8.386 0.05 1 164 140 93 ALA N N 116.246 0.2 1 165 141 94 TYR H H 9.047 0.05 1 166 141 94 TYR N N 118.310 0.2 1 167 142 95 ALA H H 7.753 0.05 1 168 142 95 ALA N N 120.359 0.2 1 169 143 96 ARG H H 8.954 0.05 1 170 143 96 ARG N N 119.188 0.2 1 171 144 97 MET H H 7.977 0.05 1 172 144 97 MET N N 118.493 0.2 1 173 145 98 GLY H H 8.418 0.05 1 174 145 98 GLY N N 105.661 0.2 1 175 146 99 HIS H H 7.872 0.05 1 176 146 99 HIS N N 119.327 0.2 1 177 147 100 ALA H H 8.548 0.05 1 178 147 100 ALA N N 121.751 0.2 1 179 148 101 LEU H H 8.385 0.05 1 180 148 101 LEU N N 116.326 0.2 1 181 149 102 GLU H H 7.765 0.05 1 182 149 102 GLU N N 117.200 0.2 1 183 150 103 GLU H H 8.055 0.05 1 184 150 103 GLU N N 117.810 0.2 1 185 151 104 ILE H H 8.499 0.05 1 186 151 104 ILE N N 118.038 0.2 1 187 152 105 LYS H H 7.399 0.05 1 188 152 105 LYS N N 120.175 0.2 1 189 153 106 LYS H H 7.421 0.05 1 190 153 106 LYS N N 115.189 0.2 1 191 154 107 PHE H H 7.843 0.05 1 192 154 107 PHE N N 116.936 0.2 1 193 155 108 LEU H H 7.274 0.05 1 194 155 108 LEU N N 115.803 0.2 1 195 156 109 ILE H H 6.929 0.05 1 196 156 109 ILE N N 118.120 0.2 1 197 158 111 ASP H H 6.324 0.05 1 198 158 111 ASP N N 119.166 0.2 1 199 159 112 TYR H H 7.850 0.05 1 200 159 112 TYR N N 119.344 0.2 1 201 160 113 ASN H H 7.910 0.05 1 202 160 113 ASN N N 124.908 0.2 1 stop_ save_