data_27303 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H, 13C and 15N backbone chemical shift assignments of A. thaliana DREB2A(255-272) ; _BMRB_accession_number 27303 _BMRB_flat_file_name bmr27303.str _Entry_type original _Submission_date 2017-11-07 _Accession_date 2017-11-07 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Staby Lasse . . 2 Skriver Karen B. . 3 Kragelund Birthe B. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 21 "13C chemical shifts" 55 "15N chemical shifts" 20 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-05-25 update BMRB 'update entry citation' 2018-04-13 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 34152 'Chemical shift assignments of DREB2A in complex with RCD1' stop_ _Original_release_date 2017-11-07 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structure of Radical-induced Cell Death1 hub domain reveals a common aa-scaffold for disorder in transcriptional networks ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 29657132 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bugge Katrine . . 2 Staby Lasse . . 3 Kemplen Katherine R. . 4 O'Shea Charlotte . . 5 Bendsen Sidsel K. . 6 Jensen Michael K. . 7 Olsen Johan G. . 8 Skriver Karen . . 9 Kragelund Birthe B. . stop_ _Journal_abbreviation Structure _Journal_volume 26 _Journal_issue 5 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 734 _Page_last 746 _Year 2018 _Details . loop_ _Keyword IDP NCBD NMR PAH RCD1 SAXS TAFH 'Transcription factor' stop_ save_ ####################################### # Cited references within the entry # ####################################### save_reference_citation _Saveframe_category citation _Citation_full . _Citation_title ; Structures and Short Linear Motif of Disordered Transcription Factor Regions Provide Clues to the Interactome of the Cellular Hub Protein Radical-induced Cell Death1 ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 27881680 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 O'Shea Charlotte . . 2 Staby Lasse . . 3 Bendsen Sidsel K. . 4 Tidemand Frederik G. . 5 Redsted Andreas . . 6 Willemoes Martin . . 7 Kragelund Birthe B. . 8 Skriver Karen . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full . _Journal_volume 292 _Journal_issue 2 _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 512 _Page_last 527 _Year 2017 _Details . loop_ _Keyword SLiM 'coupled folding and binding' interactome 'intrinsically disordered protein' 'plant stress' 'protein-protein interaction' thermodynamics stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name DREB2A(255-272) _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label DREB2A $DREB2A(255-272) stop_ _System_molecular_weight 2113.1 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details DREB2A(255-272) save_ ######################## # Monomeric polymers # ######################## save_DREB2A(255-272) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common DREB2A(255-272) _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function 'heat acclimation' 'positive regulation of transcription' 'regulation of transcription' 'response to UV-B' 'response to heat' 'response to hydrogen peroxide' 'response to water deprivation' transcription stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 19 _Mol_residue_sequence ; GSSDMFDVDELLRDLNGDD ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 254 GLY 2 255 SER 3 256 SER 4 257 ASP 5 258 MET 6 259 PHE 7 260 ASP 8 261 VAL 9 262 ASP 10 263 GLU 11 264 LEU 12 265 LEU 13 266 ARG 14 267 ASP 15 268 LEU 16 269 ASN 17 270 GLY 18 271 ASP 19 272 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $DREB2A(255-272) 'Thale cress' 3702 Eukaryota Viridiplantae Arabidopsis thaliana stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $DREB2A(255-272) 'recombinant technology' . Escherichia coli . pGEX-4T-1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DREB2A(255-272) 150 uM '[U-100% 13C; U-100% 15N]' 'sodium chloride' 100 mM 'natural abundance' DSS 0.7 mM 'natural abundance' D2O 10 % 'natural abundance' PMSF 0.1 mM 'natural abundance' 'sodium azide' 0.02 '% w/v' 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CCPN_Analysis _Saveframe_category software _Name CCPN_Analysis _Version 2.4.2 loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ save_NMRDraw _Saveframe_category software _Name NMRDraw _Version 8.9 loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_Mdd_NMR _Saveframe_category software _Name Mdd_NMR _Version 2.5 loop_ _Vendor _Address _Electronic_address 'Orekhov, Jaravine, Mayzel, Kazimierczuk' . . stop_ loop_ _Task processing stop_ _Details . save_ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version 3.5 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 750 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HN(CA)CO_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)CO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CCPN_Analysis $NMRDraw $Mdd_NMR $TOPSPIN stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D CBCA(CO)NH' '3D HNCO' '3D HN(CA)CO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name DREB2A _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 255 2 SER H H 8.774 0.014 1 2 255 2 SER C C 174.987 0.006 1 3 255 2 SER CA C 58.969 0.111 1 4 255 2 SER CB C 63.752 0.062 1 5 255 2 SER N N 116.144 0.181 1 6 256 3 SER H H 8.542 0.015 1 7 256 3 SER C C 174.243 0.007 1 8 256 3 SER CA C 58.669 0.018 1 9 256 3 SER CB C 63.671 0.021 1 10 256 3 SER N N 117.368 0.210 1 11 257 4 ASP H H 8.176 0.013 1 12 257 4 ASP C C 175.991 0.005 1 13 257 4 ASP CA C 54.531 0.083 1 14 257 4 ASP CB C 41.032 0.055 1 15 257 4 ASP N N 121.767 0.150 1 16 258 5 MET H H 8.102 0.013 1 17 258 5 MET C C 175.732 0.002 1 18 258 5 MET CA C 55.630 0.027 1 19 258 5 MET CB C 32.678 0.076 1 20 258 5 MET N N 119.584 0.199 1 21 259 6 PHE H H 8.098 0.014 1 22 259 6 PHE C C 175.308 0.002 1 23 259 6 PHE CA C 57.486 0.119 1 24 259 6 PHE CB C 39.602 0.056 1 25 259 6 PHE N N 120.363 0.182 1 26 260 7 ASP H H 8.323 0.017 1 27 260 7 ASP C C 176.199 0.000 1 28 260 7 ASP CA C 54.061 0.079 1 29 260 7 ASP CB C 41.038 0.012 1 30 260 7 ASP N N 122.024 0.171 1 31 261 8 VAL H H 8.068 0.011 1 32 261 8 VAL C C 176.163 0.004 1 33 261 8 VAL CA C 62.765 0.099 1 34 261 8 VAL CB C 32.708 0.091 1 35 261 8 VAL N N 120.160 0.182 1 36 262 9 ASP H H 8.325 0.014 1 37 262 9 ASP C C 176.904 0.000 1 38 262 9 ASP CA C 54.975 0.052 1 39 262 9 ASP CB C 40.987 0.014 1 40 262 9 ASP N N 122.973 0.139 1 41 263 10 GLU H H 8.277 0.017 1 42 263 10 GLU C C 176.918 0.005 1 43 263 10 GLU CA C 57.522 0.077 1 44 263 10 GLU CB C 29.858 0.005 1 45 263 10 GLU N N 121.608 0.128 1 46 264 11 LEU H H 8.122 0.019 1 47 264 11 LEU C C 177.868 0.009 1 48 264 11 LEU CA C 55.954 0.123 1 49 264 11 LEU CB C 41.901 0.039 1 50 264 11 LEU N N 121.265 0.164 1 51 265 12 LEU H H 7.994 0.018 1 52 265 12 LEU C C 177.564 0.007 1 53 265 12 LEU CA C 55.293 0.099 1 54 265 12 LEU CB C 41.805 0.065 1 55 265 12 LEU N N 121.244 0.175 1 56 266 13 ARG H H 7.951 0.014 1 57 266 13 ARG HE H 7.274 0.009 1 58 266 13 ARG C C 176.234 0.017 1 59 266 13 ARG CA C 56.582 0.076 1 60 266 13 ARG CB C 30.965 0.037 1 61 266 13 ARG CG C 27.073 0.000 1 62 266 13 ARG CD C 43.416 0.036 1 63 266 13 ARG N N 121.142 0.153 1 64 266 13 ARG NE N 84.869 0.000 1 65 267 14 ASP H H 8.318 0.015 1 66 267 14 ASP C C 176.467 0.013 1 67 267 14 ASP CA C 54.408 0.081 1 68 267 14 ASP CB C 40.834 0.024 1 69 267 14 ASP N N 120.895 0.141 1 70 268 15 LEU H H 8.180 0.014 1 71 268 15 LEU C C 177.550 0.005 1 72 268 15 LEU CA C 55.496 0.085 1 73 268 15 LEU CB C 42.079 0.081 1 74 268 15 LEU N N 122.848 0.172 1 75 269 16 ASN H H 8.431 0.019 1 76 269 16 ASN HD21 H 7.688 0.000 1 77 269 16 ASN HD22 H 6.900 0.000 1 78 269 16 ASN C C 175.687 0.001 1 79 269 16 ASN CA C 53.533 0.046 1 80 269 16 ASN CB C 39.209 0.051 1 81 269 16 ASN N N 118.753 0.149 1 82 269 16 ASN ND2 N 113.518 0.001 1 83 270 17 GLY H H 8.256 0.017 1 84 270 17 GLY C C 173.854 0.000 1 85 270 17 GLY CA C 45.497 0.071 1 86 270 17 GLY N N 109.329 0.178 1 87 271 18 ASP H H 8.282 0.015 1 88 271 18 ASP C C 175.172 0.004 1 89 271 18 ASP CA C 54.239 0.052 1 90 271 18 ASP CB C 41.227 0.010 1 91 271 18 ASP N N 120.854 0.145 1 92 272 19 ASP H H 7.968 0.013 1 93 272 19 ASP C C 180.588 0.000 1 94 272 19 ASP CA C 55.631 0.000 1 95 272 19 ASP CB C 41.830 0.000 1 96 272 19 ASP N N 125.403 0.130 1 stop_ save_