data_27323 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; C-terminal domain of Cdc37 cochaperone ; _BMRB_accession_number 27323 _BMRB_flat_file_name bmr27323.str _Entry_type original _Submission_date 2017-12-03 _Accession_date 2017-12-03 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bachman Ashleigh . . 2 Keramisanou Dimitra . . 3 'Kumar M.V.' Vasantha . . 4 Gelis Ioannis . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 79 "13C chemical shifts" 243 "15N chemical shifts" 80 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-03-22 update BMRB 'update entry citation' 2018-01-16 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 25740 'C-terminal domain of Cdc37 cochaperone' 27322 'C-terminal domain of Cdc37 (Y298E mutant)' 27324 'C-terminal domain of Cdc37 (unfolded)' stop_ _Original_release_date 2017-12-04 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 29343704 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bachman Ashleigh B. . 2 Keramisanou Dimitra . . 3 Xu Wanping . . 4 Beebe Kristin . . 5 Moses Michael A. . 6 'Vasantha Kumar' M V. . 7 Gray Geoffrey . . 8 Noor 'Radwan Ebna' E. . 9 'van der Vaart' Arjan . . 10 Neckers Len . . 11 Gelis Ioannis . . stop_ _Journal_abbreviation 'Nat. Commun.' _Journal_name_full 'Nature communications' _Journal_volume 9 _Journal_issue 1 _Journal_ISSN 2041-1723 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 265 _Page_last 265 _Year 2018 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'C-terminal domain of Cdc37' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'C-terminal domain of Cdc37' $Cdc37 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Cdc37 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Cdc37 _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 93 _Mol_residue_sequence ; GMGPGGLDPVEVFESLPEEL QKCFDVKDVQMLQDAISKMD PTDAKYHMQRCIDSGLWVPN SKASEAKEGEEAGPGDPLLE AVPKTGDEKDVSV ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 286 GLY 2 287 MET 3 288 GLY 4 289 PRO 5 290 GLY 6 291 GLY 7 292 LEU 8 293 ASP 9 294 PRO 10 295 VAL 11 296 GLU 12 297 VAL 13 298 PHE 14 299 GLU 15 300 SER 16 301 LEU 17 302 PRO 18 303 GLU 19 304 GLU 20 305 LEU 21 306 GLN 22 307 LYS 23 308 CYS 24 309 PHE 25 310 ASP 26 311 VAL 27 312 LYS 28 313 ASP 29 314 VAL 30 315 GLN 31 316 MET 32 317 LEU 33 318 GLN 34 319 ASP 35 320 ALA 36 321 ILE 37 322 SER 38 323 LYS 39 324 MET 40 325 ASP 41 326 PRO 42 327 THR 43 328 ASP 44 329 ALA 45 330 LYS 46 331 TYR 47 332 HIS 48 333 MET 49 334 GLN 50 335 ARG 51 336 CYS 52 337 ILE 53 338 ASP 54 339 SER 55 340 GLY 56 341 LEU 57 342 TRP 58 343 VAL 59 344 PRO 60 345 ASN 61 346 SER 62 347 LYS 63 348 ALA 64 349 SER 65 350 GLU 66 351 ALA 67 352 LYS 68 353 GLU 69 354 GLY 70 355 GLU 71 356 GLU 72 357 ALA 73 358 GLY 74 359 PRO 75 360 GLY 76 361 ASP 77 362 PRO 78 363 LEU 79 364 LEU 80 365 GLU 81 366 ALA 82 367 VAL 83 368 PRO 84 369 LYS 85 370 THR 86 371 GLY 87 372 ASP 88 373 GLU 89 374 LYS 90 375 ASP 91 376 VAL 92 377 SER 93 378 VAL stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Cdc37 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Cdc37 'recombinant technology' . Escherichia coli . PDB.HIS.MBP stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Cdc37 0.5 mM '[U-100% 13C; U-100% 15N]' stop_ save_ save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Cdc37 0.5 mM '[U-100% 15N]' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Agilent _Model 'direct drive' _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_2 save_ save_3D_HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_2 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_2 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 7.5 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.7 internal direct . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCA' '3D HNCACB' '3D HN(CO)CA' stop_ loop_ _Sample_label $sample_1 $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'C-terminal domain of Cdc37' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 288 3 GLY H H 8.317 . . 2 288 3 GLY CA C 44.754 . . 3 288 3 GLY N N 109.218 . . 4 289 4 PRO CA C 64.133 . . 5 289 4 PRO CB C 31.974 . . 6 290 5 GLY H H 8.575 . . 7 290 5 GLY CA C 45.234 . . 8 290 5 GLY N N 108.131 . . 9 291 6 GLY H H 8.092 . . 10 291 6 GLY C C 173.474 . . 11 291 6 GLY CA C 45.545 . . 12 291 6 GLY N N 107.726 . . 13 292 7 LEU H H 7.337 . . 14 292 7 LEU C C 175.458 . . 15 292 7 LEU CA C 53.655 . . 16 292 7 LEU CB C 44.546 . . 17 292 7 LEU N N 119.088 . . 18 293 8 ASP H H 8.756 . . 19 293 8 ASP CA C 51.275 . . 20 293 8 ASP CB C 43.064 . . 21 293 8 ASP N N 124.469 . . 22 294 9 PRO C C 178.378 . . 23 294 9 PRO CA C 65.025 . . 24 294 9 PRO CB C 31.807 . . 25 295 10 VAL H H 8.209 . . 26 295 10 VAL C C 177.812 . . 27 295 10 VAL CA C 66.364 . . 28 295 10 VAL CB C 31.389 . . 29 295 10 VAL N N 121.470 . . 30 296 11 GLU H H 7.245 . . 31 296 11 GLU C C 180.091 . . 32 296 11 GLU CA C 58.854 . . 33 296 11 GLU CB C 29.974 . . 34 296 11 GLU N N 120.439 . . 35 297 12 VAL H H 8.288 . . 36 297 12 VAL C C 178.999 . . 37 297 12 VAL CA C 66.265 . . 38 297 12 VAL CB C 31.255 . . 39 297 12 VAL N N 118.197 . . 40 298 13 PHE H H 8.652 . . 41 298 13 PHE C C 177.406 . . 42 298 13 PHE CA C 62.048 . . 43 298 13 PHE CB C 39.503 . . 44 298 13 PHE N N 122.449 . . 45 299 14 GLU H H 8.147 . . 46 299 14 GLU C C 176.580 . . 47 299 14 GLU CA C 58.483 . . 48 299 14 GLU CB C 29.721 . . 49 299 14 GLU N N 115.833 . . 50 300 15 SER H H 7.713 . . 51 300 15 SER C C 173.990 . . 52 300 15 SER CA C 58.502 . . 53 300 15 SER CB C 64.809 . . 54 300 15 SER N N 113.074 . . 55 301 16 LEU H H 7.339 . . 56 301 16 LEU CA C 53.076 . . 57 301 16 LEU CB C 41.794 . . 58 301 16 LEU N N 124.263 . . 59 302 17 PRO C C 177.973 . . 60 302 17 PRO CA C 62.350 . . 61 302 17 PRO CB C 32.420 . . 62 302 17 PRO N N 124.931 . . 63 303 18 GLU H H 8.983 . . 64 303 18 GLU C C 177.713 . . 65 303 18 GLU CA C 60.172 . . 66 303 18 GLU CB C 29.810 . . 67 303 18 GLU N N 125.879 . . 68 304 19 GLU H H 9.343 . . 69 304 19 GLU C C 178.803 . . 70 304 19 GLU CA C 59.729 . . 71 304 19 GLU CB C 29.271 . . 72 304 19 GLU N N 116.549 . . 73 305 20 LEU H H 7.182 . . 74 305 20 LEU C C 178.449 . . 75 305 20 LEU CA C 56.751 . . 76 305 20 LEU CB C 42.422 . . 77 305 20 LEU N N 115.718 . . 78 306 21 GLN H H 7.928 . . 79 306 21 GLN C C 178.706 . . 80 306 21 GLN CA C 59.791 . . 81 306 21 GLN CB C 28.419 . . 82 306 21 GLN N N 119.064 . . 83 307 22 LYS H H 7.843 . . 84 307 22 LYS C C 178.345 . . 85 307 22 LYS CA C 58.703 . . 86 307 22 LYS CB C 32.333 . . 87 307 22 LYS N N 115.572 . . 88 308 23 CYS H H 7.346 . . 89 308 23 CYS C C 176.308 . . 90 308 23 CYS CA C 61.693 . . 91 308 23 CYS CB C 27.629 . . 92 308 23 CYS N N 115.389 . . 93 309 24 PHE H H 7.787 . . 94 309 24 PHE C C 176.851 . . 95 309 24 PHE CA C 60.855 . . 96 309 24 PHE CB C 39.096 . . 97 309 24 PHE N N 117.888 . . 98 310 25 ASP H H 8.177 . . 99 310 25 ASP CA C 56.688 . . 100 310 25 ASP CB C 41.790 . . 101 310 25 ASP N N 121.280 . . 102 311 26 VAL C C 175.805 . . 103 311 26 VAL CA C 62.029 . . 104 311 26 VAL CB C 32.385 . . 105 312 27 LYS H H 7.907 . . 106 312 27 LYS C C 175.161 . . 107 312 27 LYS CA C 57.308 . . 108 312 27 LYS CB C 30.255 . . 109 312 27 LYS N N 118.979 . . 110 313 28 ASP H H 7.465 . . 111 313 28 ASP C C 176.460 . . 112 313 28 ASP CA C 53.397 . . 113 313 28 ASP CB C 42.753 . . 114 313 28 ASP N N 119.145 . . 115 314 29 VAL H H 8.496 . . 116 314 29 VAL C C 177.296 . . 117 314 29 VAL CA C 65.004 . . 118 314 29 VAL CB C 31.977 . . 119 314 29 VAL N N 123.855 . . 120 315 30 GLN H H 8.232 . . 121 315 30 GLN C C 178.142 . . 122 315 30 GLN CA C 58.611 . . 123 315 30 GLN CB C 28.259 . . 124 315 30 GLN N N 122.217 . . 125 316 31 MET H H 8.109 . . 126 316 31 MET C C 178.762 . . 127 316 31 MET CA C 58.521 . . 128 316 31 MET CB C 33.122 . . 129 316 31 MET N N 120.202 . . 130 317 32 LEU H H 7.665 . . 131 317 32 LEU C C 177.612 . . 132 317 32 LEU CA C 58.267 . . 133 317 32 LEU CB C 41.846 . . 134 317 32 LEU N N 120.954 . . 135 318 33 GLN H H 8.029 . . 136 318 33 GLN C C 178.921 . . 137 318 33 GLN CA C 59.014 . . 138 318 33 GLN CB C 28.225 . . 139 318 33 GLN N N 117.520 . . 140 319 34 ASP H H 8.197 . . 141 319 34 ASP C C 178.399 . . 142 319 34 ASP CA C 57.014 . . 143 319 34 ASP CB C 40.830 . . 144 319 34 ASP N N 120.300 . . 145 320 35 ALA H H 8.131 . . 146 320 35 ALA C C 181.246 . . 147 320 35 ALA CA C 55.300 . . 148 320 35 ALA CB C 18.598 . . 149 320 35 ALA N N 122.964 . . 150 321 36 ILE H H 8.453 . . 151 321 36 ILE C C 178.748 . . 152 321 36 ILE CA C 63.973 . . 153 321 36 ILE CB C 37.666 . . 154 321 36 ILE N N 117.997 . . 155 322 37 SER H H 7.913 . . 156 322 37 SER C C 174.803 . . 157 322 37 SER CA C 61.174 . . 158 322 37 SER CB C 63.340 . . 159 322 37 SER N N 115.581 . . 160 323 38 LYS H H 7.160 . . 161 323 38 LYS C C 176.698 . . 162 323 38 LYS CA C 56.363 . . 163 323 38 LYS CB C 33.338 . . 164 323 38 LYS N N 119.296 . . 165 324 39 MET H H 7.404 . . 166 324 39 MET C C 175.835 . . 167 324 39 MET CA C 55.611 . . 168 324 39 MET CB C 35.534 . . 169 324 39 MET N N 118.977 . . 170 325 40 ASP H H 8.949 . . 171 325 40 ASP CA C 53.124 . . 172 325 40 ASP CB C 42.256 . . 173 325 40 ASP N N 124.344 . . 174 326 41 PRO C C 179.065 . . 175 326 41 PRO CA C 65.502 . . 176 326 41 PRO CB C 32.314 . . 177 327 42 THR H H 8.439 . . 178 327 42 THR C C 176.673 . . 179 327 42 THR CA C 66.288 . . 180 327 42 THR CB C 68.021 . . 181 327 42 THR N N 115.189 . . 182 328 43 ASP H H 7.760 . . 183 328 43 ASP C C 177.633 . . 184 328 43 ASP CA C 57.049 . . 185 328 43 ASP CB C 41.118 . . 186 328 43 ASP N N 124.300 . . 187 329 44 ALA H H 8.704 . . 188 329 44 ALA C C 179.225 . . 189 329 44 ALA CA C 55.824 . . 190 329 44 ALA CB C 19.422 . . 191 329 44 ALA N N 121.425 . . 192 330 45 LYS H H 7.866 . . 193 330 45 LYS C C 178.346 . . 194 330 45 LYS CA C 59.526 . . 195 330 45 LYS CB C 32.583 . . 196 330 45 LYS N N 116.615 . . 197 331 46 TYR H H 7.556 . . 198 331 46 TYR C C 177.802 . . 199 331 46 TYR CA C 60.873 . . 200 331 46 TYR CB C 38.267 . . 201 331 46 TYR N N 119.688 . . 202 332 47 HIS H H 8.236 . . 203 332 47 HIS C C 178.914 . . 204 332 47 HIS CA C 59.909 . . 205 332 47 HIS CB C 31.187 . . 206 332 47 HIS N N 116.997 . . 207 333 48 MET H H 8.891 . . 208 333 48 MET C C 178.585 . . 209 333 48 MET CA C 58.277 . . 210 333 48 MET CB C 31.418 . . 211 333 48 MET N N 118.438 . . 212 334 49 GLN H H 8.194 . . 213 334 49 GLN C C 178.190 . . 214 334 49 GLN CA C 58.643 . . 215 334 49 GLN CB C 27.980 . . 216 334 49 GLN N N 118.709 . . 217 335 50 ARG H H 7.268 . . 218 335 50 ARG C C 180.085 . . 219 335 50 ARG CA C 59.388 . . 220 335 50 ARG CB C 29.935 . . 221 335 50 ARG N N 118.088 . . 222 336 51 CYS H H 8.354 . . 223 336 51 CYS C C 176.482 . . 224 336 51 CYS CA C 64.902 . . 225 336 51 CYS CB C 27.036 . . 226 336 51 CYS N N 120.436 . . 227 337 52 ILE H H 8.373 . . 228 337 52 ILE C C 180.302 . . 229 337 52 ILE CA C 64.645 . . 230 337 52 ILE CB C 38.659 . . 231 337 52 ILE N N 122.686 . . 232 338 53 ASP H H 9.002 . . 233 338 53 ASP C C 177.997 . . 234 338 53 ASP CA C 57.340 . . 235 338 53 ASP CB C 40.679 . . 236 338 53 ASP N N 121.564 . . 237 339 54 SER H H 7.776 . . 238 339 54 SER C C 174.923 . . 239 339 54 SER CA C 58.699 . . 240 339 54 SER CB C 65.363 . . 241 339 54 SER N N 110.788 . . 242 340 55 GLY H H 7.774 . . 243 340 55 GLY C C 173.795 . . 244 340 55 GLY CA C 45.632 . . 245 340 55 GLY N N 110.155 . . 246 341 56 LEU H H 8.033 . . 247 341 56 LEU C C 176.377 . . 248 341 56 LEU CA C 56.897 . . 249 341 56 LEU CB C 43.267 . . 250 341 56 LEU N N 121.523 . . 251 342 57 TRP H H 7.470 . . 252 342 57 TRP CA C 55.997 . . 253 342 57 TRP CB C 32.382 . . 254 342 57 TRP N N 116.901 . . 255 343 58 VAL H H 7.752 . . 256 343 58 VAL CA C 58.872 . . 257 343 58 VAL CB C 32.802 . . 258 343 58 VAL N N 125.694 . . 259 347 62 LYS C C 176.482 . . 260 347 62 LYS CA C 56.362 . . 261 347 62 LYS CB C 32.986 . . 262 348 63 ALA H H 8.270 . . 263 348 63 ALA C C 177.945 . . 264 348 63 ALA CA C 52.928 . . 265 348 63 ALA CB C 19.195 . . 266 348 63 ALA N N 125.303 . . 267 349 64 SER H H 8.217 . . 268 349 64 SER C C 174.576 . . 269 349 64 SER CA C 58.577 . . 270 349 64 SER CB C 63.814 . . 271 349 64 SER N N 114.702 . . 272 350 65 GLU H H 8.307 . . 273 350 65 GLU C C 176.046 . . 274 350 65 GLU CA C 56.512 . . 275 350 65 GLU CB C 30.454 . . 276 350 65 GLU N N 122.634 . . 277 351 66 ALA H H 8.211 . . 278 351 66 ALA C C 177.484 . . 279 351 66 ALA CA C 52.518 . . 280 351 66 ALA CB C 19.359 . . 281 351 66 ALA N N 125.103 . . 282 352 67 LYS H H 8.309 . . 283 352 67 LYS C C 176.521 . . 284 352 67 LYS CA C 56.010 . . 285 352 67 LYS CB C 33.266 . . 286 352 67 LYS N N 121.414 . . 287 353 68 GLU H H 8.485 . . 288 353 68 GLU C C 177.019 . . 289 353 68 GLU CA C 56.982 . . 290 353 68 GLU CB C 30.319 . . 291 353 68 GLU N N 122.549 . . 292 354 69 GLY H H 8.504 . . 293 354 69 GLY C C 174.192 . . 294 354 69 GLY CA C 45.395 . . 295 354 69 GLY N N 110.613 . . 296 355 70 GLU H H 8.177 . . 297 355 70 GLU C C 176.558 . . 298 355 70 GLU CA C 56.452 . . 299 355 70 GLU CB C 30.589 . . 300 355 70 GLU N N 120.433 . . 301 356 71 GLU H H 8.522 . . 302 356 71 GLU C C 176.129 . . 303 356 71 GLU CA C 56.609 . . 304 356 71 GLU CB C 30.331 . . 305 356 71 GLU N N 122.081 . . 306 357 72 ALA H H 8.341 . . 307 357 72 ALA C C 177.879 . . 308 357 72 ALA CA C 52.531 . . 309 357 72 ALA CB C 19.602 . . 310 357 72 ALA N N 125.522 . . 311 358 73 GLY H H 8.253 . . 312 358 73 GLY CA C 44.562 . . 313 358 73 GLY N N 108.485 . . 314 359 74 PRO C C 177.599 . . 315 359 74 PRO CA C 63.456 . . 316 359 74 PRO CB C 32.254 . . 317 360 75 GLY H H 8.412 . . 318 360 75 GLY C C 173.439 . . 319 360 75 GLY CA C 44.920 . . 320 360 75 GLY N N 109.271 . . 321 361 76 ASP H H 8.127 . . 322 361 76 ASP CA C 52.356 . . 323 361 76 ASP CB C 41.396 . . 324 361 76 ASP N N 121.820 . . 325 362 77 PRO C C 177.243 . . 326 362 77 PRO CA C 63.631 . . 327 362 77 PRO CB C 32.200 . . 328 363 78 LEU H H 8.362 . . 329 363 78 LEU C C 177.621 . . 330 363 78 LEU CA C 55.532 . . 331 363 78 LEU CB C 41.764 . . 332 363 78 LEU N N 120.702 . . 333 364 79 LEU H H 7.904 . . 334 364 79 LEU C C 177.347 . . 335 364 79 LEU CA C 55.252 . . 336 364 79 LEU CB C 42.377 . . 337 364 79 LEU N N 121.784 . . 338 365 80 GLU H H 8.104 . . 339 365 80 GLU C C 175.895 . . 340 365 80 GLU CA C 56.413 . . 341 365 80 GLU CB C 30.511 . . 342 365 80 GLU N N 121.049 . . 343 366 81 ALA H H 8.148 . . 344 366 81 ALA C C 177.297 . . 345 366 81 ALA CA C 52.352 . . 346 366 81 ALA CB C 19.338 . . 347 366 81 ALA N N 124.927 . . 348 367 82 VAL H H 8.011 . . 349 367 82 VAL CA C 59.892 . . 350 367 82 VAL CB C 32.630 . . 351 367 82 VAL N N 120.936 . . 352 368 83 PRO C C 176.720 . . 353 368 83 PRO CA C 63.141 . . 354 368 83 PRO CB C 32.264 . . 355 369 84 LYS H H 8.466 . . 356 369 84 LYS C C 176.952 . . 357 369 84 LYS CA C 56.090 . . 358 369 84 LYS CB C 33.238 . . 359 369 84 LYS N N 122.339 . . 360 370 85 THR H H 8.351 . . 361 370 85 THR C C 175.116 . . 362 370 85 THR CA C 61.852 . . 363 370 85 THR CB C 70.016 . . 364 370 85 THR N N 115.423 . . 365 371 86 GLY H H 8.531 . . 366 371 86 GLY C C 173.867 . . 367 371 86 GLY CA C 45.422 . . 368 371 86 GLY N N 111.294 . . 369 372 87 ASP H H 8.260 . . 370 372 87 ASP C C 176.416 . . 371 372 87 ASP CA C 54.360 . . 372 372 87 ASP CB C 41.528 . . 373 372 87 ASP N N 120.639 . . 374 373 88 GLU H H 8.511 . . 375 373 88 GLU C C 176.567 . . 376 373 88 GLU CA C 57.155 . . 377 373 88 GLU CB C 29.965 . . 378 373 88 GLU N N 121.332 . . 379 374 89 LYS H H 8.285 . . 380 374 89 LYS C C 176.212 . . 381 374 89 LYS CA C 56.374 . . 382 374 89 LYS CB C 33.128 . . 383 374 89 LYS N N 121.529 . . 384 375 90 ASP H H 8.302 . . 385 375 90 ASP C C 176.103 . . 386 375 90 ASP CA C 54.459 . . 387 375 90 ASP CB C 41.212 . . 388 375 90 ASP N N 121.489 . . 389 376 91 VAL H H 8.004 . . 390 376 91 VAL C C 176.066 . . 391 376 91 VAL CA C 62.020 . . 392 376 91 VAL CB C 32.790 . . 393 376 91 VAL N N 119.610 . . 394 377 92 SER H H 8.406 . . 395 377 92 SER C C 173.568 . . 396 377 92 SER CA C 58.444 . . 397 377 92 SER CB C 64.028 . . 398 377 92 SER N N 120.230 . . 399 378 93 VAL H H 7.710 . . 400 378 93 VAL CA C 63.595 . . 401 378 93 VAL CB C 33.287 . . 402 378 93 VAL N N 125.751 . . stop_ save_