data_27374 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H, 13C and 15N backbone resonance assignment of the lamin C-terminal region of progerin ; _BMRB_accession_number 27374 _BMRB_flat_file_name bmr27374.str _Entry_type original _Submission_date 2018-01-22 _Accession_date 2018-01-22 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Zinn-Justin Sophie . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 45 "13C chemical shifts" 128 "15N chemical shifts" 45 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-09-25 update BMRB 'update entry citation' 2018-01-26 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 27375 'prelamin A C-terminal region' 27376 'Progerin C-terminal region' stop_ _Original_release_date 2018-01-22 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; 1H, 13C and 15N backbone resonance assignment of the lamin C-terminal region specific to prelamin A ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 29582385 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Celli Florian . . 2 Petitalot Ambre . . 3 Samson Camille . . 4 Theillet Francois-Xavier . . 5 Zinn-Justin Sophie . . stop_ _Journal_abbreviation 'Biomol. NMR Assignments' _Journal_volume 12 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 225 _Page_last 229 _Year 2018 _Details . loop_ _Keyword 'Intrinsically disordered protein' 'NMR spectroscopy.' 'Nuclear envelope' Nucleoskeleton stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Progerin C-ter' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Progerin C-ter' $Progerin_C-ter stop_ _System_molecular_weight . _System_physical_state 'intrinsically disordered' _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Progerin_C-ter _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Progerin_C-ter _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 49 _Mol_residue_sequence ; WGSHCSSSGDPAEYNLRSRT VLCGTCGQPADKASASGSGA QSPQNCSIM ; loop_ _Residue_seq_code _Residue_label 1 TRP 2 GLY 3 SER 4 HIS 5 CYS 6 SER 7 SER 8 SER 9 GLY 10 ASP 11 PRO 12 ALA 13 GLU 14 TYR 15 ASN 16 LEU 17 ARG 18 SER 19 ARG 20 THR 21 VAL 22 LEU 23 CYS 24 GLY 25 THR 26 CYS 27 GLY 28 GLN 29 PRO 30 ALA 31 ASP 32 LYS 33 ALA 34 SER 35 ALA 36 SER 37 GLY 38 SER 39 GLY 40 ALA 41 GLN 42 SER 43 PRO 44 GLN 45 ASN 46 CYS 47 SER 48 ILE 49 MET stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Progerin_C-ter Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Progerin_C-ter 'recombinant technology' . Escherichia coli . pETM-13 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Progerin_C-ter 600 uM '[U-100% 13C; U-100% 15N]' 'Phosphate Buffer' 20 mM 'Natural Abundance' NaCl 150 mM 'Natural Abundance' stop_ save_ ############################ # Computer software used # ############################ save_TINKER _Saveframe_category software _Name TINKER _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_HNCO_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CA)NNH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)NNH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 150 . mM pH 6.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 4.6 internal direct . . . 1 water H 1 protons ppm 4.6 internal direct . . . 1 water N 15 protons ppm 4.6 internal direct . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNCO' '3D HNCA' '3D HNCACB' '3D HN(CA)NNH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Progerin C-ter' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 TRP H H 8.46 0.02 1 2 1 1 TRP N N 121.06 0.25 1 3 2 2 GLY H H 8.219 0.02 1 4 2 2 GLY N N 111.33 0.25 1 5 3 3 SER H H 7.991 0.02 1 6 3 3 SER N N 115.52 0.25 1 7 4 4 HIS C C 174.431 0.80 1 8 4 4 HIS CA C 55.729 0.80 1 9 4 4 HIS CB C 29.06 0.80 1 10 5 5 CYS H H 8.126 0.02 1 11 5 5 CYS C C 174.338 0.80 1 12 5 5 CYS CA C 58.404 0.80 1 13 5 5 CYS CB C 27.895 0.80 1 14 5 5 CYS N N 120.343 0.25 1 15 6 6 SER H H 8.382 0.02 1 16 6 6 SER C C 174.496 0.80 1 17 6 6 SER CA C 58.285 0.80 1 18 6 6 SER CB C 63.575 0.80 1 19 6 6 SER N N 118.567 0.25 1 20 7 7 SER H H 8.294 0.02 1 21 7 7 SER C C 174.475 0.80 1 22 7 7 SER CA C 58.356 0.80 1 23 7 7 SER CB C 63.472 0.80 1 24 7 7 SER N N 117.905 0.25 1 25 8 8 SER H H 8.189 0.02 1 26 8 8 SER C C 174.67 0.80 1 27 8 8 SER CA C 58.472 0.80 1 28 8 8 SER CB C 63.486 0.80 1 29 8 8 SER N N 117.441 0.25 1 30 9 9 GLY H H 8.135 0.02 1 31 9 9 GLY C C 173.219 0.80 1 32 9 9 GLY CA C 44.722 0.80 1 33 9 9 GLY N N 110.579 0.25 1 34 10 10 ASP H H 8.032 0.02 1 35 10 10 ASP C C 174.882 0.80 1 36 10 10 ASP CA C 51.994 0.80 1 37 10 10 ASP CB C 40.953 0.80 1 38 10 10 ASP N N 121.867 0.25 1 39 11 11 PRO C C 177.042 0.80 1 40 11 11 PRO CA C 63.557 0.80 1 41 11 11 PRO CB C 32.084 0.80 1 42 12 12 ALA H H 8.229 0.02 1 43 12 12 ALA C C 178.345 0.80 1 44 12 12 ALA CA C 53.044 0.80 1 45 12 12 ALA CB C 18.675 0.80 1 46 12 12 ALA N N 122.531 0.25 1 47 13 13 GLU H H 7.913 0.02 1 48 13 13 GLU C C 176.621 0.80 1 49 13 13 GLU CA C 56.882 0.80 1 50 13 13 GLU CB C 29.801 0.80 1 51 13 13 GLU N N 118.393 0.25 1 52 14 14 TYR H H 7.821 0.02 1 53 14 14 TYR C C 176.013 0.80 1 54 14 14 TYR CA C 58.452 0.80 1 55 14 14 TYR CB C 38.137 0.80 1 56 14 14 TYR N N 120.26 0.25 1 57 15 15 ASN H H 8.097 0.02 1 58 15 15 ASN C C 175.278 0.80 1 59 15 15 ASN CA C 53.588 0.80 1 60 15 15 ASN CB C 38.365 0.80 1 61 15 15 ASN N N 119.419 0.25 1 62 16 16 LEU H H 7.833 0.02 1 63 16 16 LEU C C 177.751 0.80 1 64 16 16 LEU CA C 55.819 0.80 1 65 16 16 LEU CB C 41.801 0.80 1 66 16 16 LEU N N 121.567 0.25 1 67 17 17 ARG H H 7.977 0.02 1 68 17 17 ARG C C 176.56 0.80 1 69 17 17 ARG CA C 56.479 0.80 1 70 17 17 ARG CB C 30.175 0.80 1 71 17 17 ARG N N 120.203 0.25 1 72 18 18 SER H H 7.975 0.02 1 73 18 18 SER C C 174.502 0.80 1 74 18 18 SER CA C 58.459 0.80 1 75 18 18 SER CB C 63.231 0.80 1 76 18 18 SER N N 115.635 0.25 1 77 19 19 ARG H H 8.073 0.02 1 78 19 19 ARG C C 176.314 0.80 1 79 19 19 ARG CA C 56.229 0.80 1 80 19 19 ARG CB C 30.416 0.80 1 81 19 19 ARG N N 122.443 0.25 1 82 20 20 THR H H 7.986 0.02 1 83 20 20 THR C C 174.284 0.80 1 84 20 20 THR CA C 62.257 0.80 1 85 20 20 THR CB C 69.28 0.80 1 86 20 20 THR N N 115.69 0.25 1 87 21 21 VAL H H 8.018 0.02 1 88 21 21 VAL C C 175.87 0.80 1 89 21 21 VAL CA C 62.214 0.80 1 90 21 21 VAL CB C 32.425 0.80 1 91 21 21 VAL N N 123.315 0.25 1 92 22 22 LEU H H 8.209 0.02 1 93 22 22 LEU C C 177.072 0.80 1 94 22 22 LEU CA C 54.874 0.80 1 95 22 22 LEU CB C 41.913 0.80 1 96 22 22 LEU N N 125.925 0.25 1 97 23 23 CYS H H 8.214 0.02 1 98 23 23 CYS C C 174.825 0.80 1 99 23 23 CYS CA C 58.512 0.80 1 100 23 23 CYS CB C 27.943 0.80 1 101 23 23 CYS N N 120.216 0.25 1 102 24 24 GLY H H 8.345 0.02 1 103 24 24 GLY C C 174.158 0.80 1 104 24 24 GLY CA C 45.169 0.80 1 105 24 24 GLY N N 111.347 0.25 1 106 25 25 THR H H 8 0.02 1 107 25 25 THR C C 174.617 0.80 1 108 25 25 THR CA C 61.683 0.80 1 109 25 25 THR CB C 69.497 0.80 1 110 25 25 THR N N 113.309 0.25 1 111 26 26 CYS H H 8.329 0.02 1 112 26 26 CYS C C 174.84 0.80 1 113 26 26 CYS CA C 58.539 0.80 1 114 26 26 CYS CB C 27.745 0.80 1 115 26 26 CYS N N 121.068 0.25 1 116 27 27 GLY H H 8.337 0.02 1 117 27 27 GLY CA C 45.02 0.80 1 118 27 27 GLY N N 111.085 0.25 1 119 28 28 GLN H H 8.064 0.02 1 120 28 28 GLN C C 173.854 0.80 1 121 28 28 GLN CA C 53.41 0.80 1 122 28 28 GLN CB C 28.645 0.80 1 123 28 28 GLN N N 120.602 0.25 1 124 29 29 PRO CA C 62.919 0.80 1 125 29 29 PRO CB C 31.931 0.80 1 126 30 30 ALA H H 8.339 0.02 1 127 30 30 ALA C C 177.493 0.80 1 128 30 30 ALA CA C 52.433 0.80 1 129 30 30 ALA CB C 19.007 0.80 1 130 30 30 ALA N N 124.111 0.25 1 131 31 31 ASP H H 8.139 0.02 1 132 31 31 ASP C C 176.277 0.80 1 133 31 31 ASP CA C 53.985 0.80 1 134 31 31 ASP CB C 40.88 0.80 1 135 31 31 ASP N N 119.191 0.25 1 136 32 32 LYS H H 8.07 0.02 1 137 32 32 LYS C C 176.479 0.80 1 138 32 32 LYS CA C 56.355 0.80 1 139 32 32 LYS CB C 32.598 0.80 1 140 32 32 LYS N N 121.766 0.25 1 141 33 33 ALA H H 8.16 0.02 1 142 33 33 ALA C C 177.953 0.80 1 143 33 33 ALA CA C 52.627 0.80 1 144 33 33 ALA CB C 18.961 0.80 1 145 33 33 ALA N N 124.531 0.25 1 146 34 34 SER H H 8.053 0.02 1 147 34 34 SER C C 174.443 0.80 1 148 34 34 SER CA C 58.226 0.80 1 149 34 34 SER CB C 63.462 0.80 1 150 34 34 SER N N 114.804 0.25 1 151 35 35 ALA H H 8.18 0.02 1 152 35 35 ALA C C 177.786 0.80 1 153 35 35 ALA CA C 52.532 0.80 1 154 35 35 ALA CB C 19.018 0.80 1 155 35 35 ALA N N 126.028 0.25 1 156 36 36 SER H H 8.131 0.02 1 157 36 36 SER C C 173.97 0.80 1 158 36 36 SER CA C 58.381 0.80 1 159 36 36 SER CB C 63.489 0.80 1 160 36 36 SER N N 114.729 0.25 1 161 37 37 GLY H H 8.225 0.02 1 162 37 37 GLY C C 174.247 0.80 1 163 37 37 GLY CA C 45.143 0.80 1 164 37 37 GLY N N 110.88 0.25 1 165 38 38 SER H H 8.153 0.02 1 166 38 38 SER C C 175.045 0.80 1 167 38 38 SER CA C 58.566 0.80 1 168 38 38 SER CB C 63.521 0.80 1 169 38 38 SER N N 115.643 0.25 1 170 39 39 GLY H H 8.336 0.02 1 171 39 39 GLY C C 173.816 0.80 1 172 39 39 GLY CA C 45.01 0.80 1 173 39 39 GLY N N 111.012 0.25 1 174 40 40 ALA H H 7.994 0.02 1 175 40 40 ALA C C 177.621 0.80 1 176 40 40 ALA CA C 52.442 0.80 1 177 40 40 ALA CB C 18.992 0.80 1 178 40 40 ALA N N 123.619 0.25 1 179 41 41 GLN H H 8.236 0.02 1 180 41 41 GLN C C 175.686 0.80 1 181 41 41 GLN CA C 55.369 0.80 1 182 41 41 GLN CB C 29.36 0.80 1 183 41 41 GLN N N 119.336 0.25 1 184 42 42 SER H H 8.238 0.02 1 185 42 42 SER C C 172.599 0.80 1 186 42 42 SER CA C 56.515 0.80 1 187 42 42 SER CB C 62.897 0.80 1 188 42 42 SER N N 118.783 0.25 1 189 43 43 PRO C C 176.875 0.80 1 190 43 43 PRO CA C 63.324 0.80 1 191 43 43 PRO CB C 31.854 0.80 1 192 44 44 GLN H H 8.329 0.02 1 193 44 44 GLN C C 175.688 0.80 1 194 44 44 GLN CA C 55.806 0.80 1 195 44 44 GLN CB C 29.207 0.80 1 196 44 44 GLN N N 119.953 0.25 1 197 45 45 ASN H H 8.289 0.02 1 198 45 45 ASN C C 174.915 0.80 1 199 45 45 ASN CA C 53.256 0.80 1 200 45 45 ASN CB C 38.529 0.80 1 201 45 45 ASN N N 119.568 0.25 1 202 46 46 CYS H H 8.168 0.02 1 203 46 46 CYS C C 174.285 0.80 1 204 46 46 CYS CA C 58.315 0.80 1 205 46 46 CYS CB C 27.978 0.80 1 206 46 46 CYS N N 119.462 0.25 1 207 47 47 SER H H 8.275 0.02 1 208 47 47 SER C C 173.958 0.80 1 209 47 47 SER CA C 58.334 0.80 1 210 47 47 SER CB C 63.451 0.80 1 211 47 47 SER N N 118.337 0.25 1 212 48 48 ILE H H 7.975 0.02 1 213 48 48 ILE C C 175.208 0.80 1 214 48 48 ILE CA C 61.263 0.80 1 215 48 48 ILE CB C 38.372 0.80 1 216 48 48 ILE N N 122.491 0.25 1 217 49 49 MET H H 7.825 0.02 1 218 49 49 MET N N 129.37 0.25 1 stop_ save_