data_27375 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H, 13C and 15N backbone resonance assignment of progerin C-terminal region without Cysteines ; _BMRB_accession_number 27375 _BMRB_flat_file_name bmr27375.str _Entry_type original _Submission_date 2018-01-22 _Accession_date 2018-01-22 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Celli Florian . . 2 Petitalot Ambre . . 3 Samson Camille . . 4 Theillet Francois-Xavier . . 5 Zinn-Justin Sophie . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 44 "13C chemical shifts" 127 "15N chemical shifts" 44 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-09-25 update BMRB 'update entry citation' 2018-01-26 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 27374 'Progerin C-ter' 27376 'prelamin A C-terminal region' stop_ _Original_release_date 2018-01-22 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; 1H, 13C and 15N backbone resonance assignment of the lamin C-terminal region specific to prelamin A ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 29582385 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Celli Florian . . 2 Petitalot Ambre . . 3 Samson Camille . . 4 Theillet Francois-Xavier . . 5 Zinn-Justin Sophie . . stop_ _Journal_abbreviation 'Biomol. NMR Assignments' _Journal_volume 12 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 225 _Page_last 229 _Year 2018 _Details . loop_ _Keyword 'Intrinsically disordered protein' 'NMR spectroscopy' 'Nuclear envelope' Nucleoskeleton stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Progerin C-terminal region' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Progerin C-terminal region' $progCterNoCys stop_ _System_molecular_weight . _System_physical_state 'intrinsically disordered' _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_progCterNoCys _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common progCterNoCys _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 48 _Mol_residue_sequence ; GSHASSSGDPAEYNLRSRTV LAGTAGQPADKASASGSGAQ SPQNASIM ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 HIS 4 ALA 5 SER 6 SER 7 SER 8 GLY 9 ASP 10 PRO 11 ALA 12 GLU 13 TYR 14 ASN 15 LEU 16 ARG 17 SER 18 ARG 19 THR 20 VAL 21 LEU 22 ALA 23 GLY 24 THR 25 ALA 26 GLY 27 GLN 28 PRO 29 ALA 30 ASP 31 LYS 32 ALA 33 SER 34 ALA 35 SER 36 GLY 37 SER 38 GLY 39 ALA 40 GLN 41 SER 42 PRO 43 GLN 44 ASN 45 ALA 46 SER 47 ILE 48 MET stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $progCterNoCys Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $progCterNoCys 'recombinant technology' . Escherichia coli . pETM-13 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $progCterNoCys 600 uM '[U-100% 13C; U-100% 15N]' 'Phosphate Buffer' 20 mM 'Natural Abundance' NaCl 150 mM 'Natural Abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_HNCO_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CA)NNH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)NNH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 150 . mM pH 6.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 4.6 internal direct . . . 1 water H 1 protons ppm 4.6 internal direct . . . 1 water N 15 protons ppm 4.6 internal direct . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNCO' '3D HNCA' '3D HNCACB' '3D HN(CA)NNH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Progerin C-terminal region' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 SER H H 8.17 0.02 1 2 2 2 SER N N 115.185 0.25 1 3 3 3 HIS H H 8.288 0.02 1 4 3 3 HIS C C 174.287 0.80 1 5 3 3 HIS CA C 55.455 0.80 1 6 3 3 HIS CB C 29.459 0.80 1 7 3 3 HIS N N 120.73 0.25 1 8 4 4 ALA H H 8.195 0.02 1 9 4 4 ALA C C 177.639 0.80 1 10 4 4 ALA CA C 52.44 0.80 1 11 4 4 ALA CB C 19.043 0.80 1 12 4 4 ALA N N 125.285 0.25 1 13 5 5 SER H H 8.299 0.02 1 14 5 5 SER C C 174.624 0.80 1 15 5 5 SER CA C 58.275 0.80 1 16 5 5 SER CB C 63.64 0.80 1 17 5 5 SER N N 115.499 0.25 1 18 6 6 SER H H 8.296 0.02 1 19 6 6 SER C C 174.541 0.80 1 20 6 6 SER CA C 58.536 0.80 1 21 6 6 SER CB C 63.716 0.80 1 22 6 6 SER N N 117.89 0.25 1 23 7 7 SER H H 8.244 0.02 1 24 7 7 SER C C 174.704 0.80 1 25 7 7 SER CA C 58.554 0.80 1 26 7 7 SER CB C 63.596 0.80 1 27 7 7 SER N N 117.566 0.25 1 28 8 8 GLY H H 8.16 0.02 1 29 8 8 GLY C C 173.277 0.80 1 30 8 8 GLY CA C 44.745 0.80 1 31 8 8 GLY N N 110.609 0.25 1 32 9 9 ASP H H 8.076 0.02 1 33 9 9 ASP C C 174.873 0.80 1 34 9 9 ASP CA C 52.152 0.80 1 35 9 9 ASP CB C 40.89 0.80 1 36 9 9 ASP N N 121.917 0.25 1 37 10 10 PRO C C 177.027 0.80 1 38 10 10 PRO CA C 63.553 0.80 1 39 10 10 PRO CB C 31.79 0.80 1 40 11 11 ALA H H 8.243 0.02 1 41 11 11 ALA C C 178.326 0.80 1 42 11 11 ALA CA C 53.037 0.80 1 43 11 11 ALA CB C 18.607 0.80 1 44 11 11 ALA N N 122.642 0.25 1 45 12 12 GLU H H 7.934 0.02 1 46 12 12 GLU C C 176.624 0.80 1 47 12 12 GLU CA C 56.845 0.80 1 48 12 12 GLU CB C 29.709 0.80 1 49 12 12 GLU N N 118.468 0.25 1 50 13 13 TYR H H 7.84 0.02 1 51 13 13 TYR C C 176.013 0.80 1 52 13 13 TYR CA C 58.549 0.80 1 53 13 13 TYR CB C 38.1 0.80 1 54 13 13 TYR N N 120.33 0.25 1 55 14 14 ASN H H 8.111 0.02 1 56 14 14 ASN C C 175.274 0.80 1 57 14 14 ASN CA C 53.535 0.80 1 58 14 14 ASN CB C 38.386 0.80 1 59 14 14 ASN N N 119.539 0.25 1 60 15 15 LEU H H 7.845 0.02 1 61 15 15 LEU C C 177.761 0.80 1 62 15 15 LEU CA C 55.923 0.80 1 63 15 15 LEU CB C 41.757 0.80 1 64 15 15 LEU N N 121.622 0.25 1 65 16 16 ARG H H 7.991 0.02 1 66 16 16 ARG C C 176.582 0.80 1 67 16 16 ARG CA C 56.517 0.80 1 68 16 16 ARG CB C 30.177 0.80 1 69 16 16 ARG N N 120.317 0.25 1 70 17 17 SER H H 7.989 0.02 1 71 17 17 SER C C 174.531 0.80 1 72 17 17 SER CA C 58.616 0.80 1 73 17 17 SER CB C 63.361 0.80 1 74 17 17 SER N N 115.749 0.25 1 75 18 18 ARG H H 8.09 0.02 1 76 18 18 ARG C C 176.387 0.80 1 77 18 18 ARG CA C 56.297 0.80 1 78 18 18 ARG CB C 30.394 0.80 1 79 18 18 ARG N N 122.501 0.25 1 80 19 19 THR H H 8.006 0.02 1 81 19 19 THR C C 174.309 0.80 1 82 19 19 THR CA C 62.354 0.80 1 83 19 19 THR CB C 69.536 0.80 1 84 19 19 THR N N 115.976 0.25 1 85 20 20 VAL H H 8.052 0.02 1 86 20 20 VAL C C 175.968 0.80 1 87 20 20 VAL CA C 62.318 0.80 1 88 20 20 VAL CB C 32.382 0.80 1 89 20 20 VAL N N 123.643 0.25 1 90 21 21 LEU H H 8.19 0.02 1 91 21 21 LEU C C 176.92 0.80 1 92 21 21 LEU CA C 54.824 0.80 1 93 21 21 LEU CB C 41.95 0.80 1 94 21 21 LEU N N 126.265 0.25 1 95 22 22 ALA H H 8.167 0.02 1 96 22 22 ALA C C 178.099 0.80 1 97 22 22 ALA CA C 52.577 0.80 1 98 22 22 ALA CB C 18.953 0.80 1 99 22 22 ALA N N 125.227 0.25 1 100 23 23 GLY H H 8.226 0.02 1 101 23 23 GLY C C 174.375 0.80 1 102 23 23 GLY CA C 45.113 0.80 1 103 23 23 GLY N N 108.117 0.25 1 104 24 24 THR H H 7.896 0.02 1 105 24 24 THR C C 174.444 0.80 1 106 24 24 THR CA C 61.682 0.80 1 107 24 24 THR CB C 69.738 0.80 1 108 24 24 THR N N 113.275 0.25 1 109 25 25 ALA H H 8.256 0.02 1 110 25 25 ALA C C 178.046 0.80 1 111 25 25 ALA CA C 52.651 0.80 1 112 25 25 ALA CB C 18.872 0.80 1 113 25 25 ALA N N 126.388 0.25 1 114 26 26 GLY H H 8.232 0.02 1 115 26 26 GLY C C 173.646 0.80 1 116 26 26 GLY CA C 44.849 0.80 1 117 26 26 GLY N N 108.1 0.25 1 118 27 27 GLN H H 8.014 0.02 1 119 27 27 GLN C C 173.889 0.80 1 120 27 27 GLN CA C 53.394 0.80 1 121 27 27 GLN CB C 28.491 0.80 1 122 27 27 GLN N N 120.549 0.25 1 123 28 28 PRO CA C 62.98 0.80 1 124 28 28 PRO CB C 31.856 0.80 1 125 29 29 ALA H H 8.349 0.02 1 126 29 29 ALA C C 177.515 0.80 1 127 29 29 ALA CA C 52.422 0.80 1 128 29 29 ALA CB C 18.881 0.80 1 129 29 29 ALA N N 124.192 0.25 1 130 30 30 ASP H H 8.15 0.02 1 131 30 30 ASP C C 176.285 0.80 1 132 30 30 ASP CA C 54.101 0.80 1 133 30 30 ASP CB C 40.944 0.80 1 134 30 30 ASP N N 119.25 0.25 1 135 31 31 LYS H H 8.077 0.02 1 136 31 31 LYS C C 176.49 0.80 1 137 31 31 LYS CA C 56.464 0.80 1 138 31 31 LYS CB C 32.62 0.80 1 139 31 31 LYS N N 121.84 0.25 1 140 32 32 ALA H H 8.169 0.02 1 141 32 32 ALA C C 177.957 0.80 1 142 32 32 ALA CA C 52.675 0.80 1 143 32 32 ALA CB C 18.819 0.80 1 144 32 32 ALA N N 124.579 0.25 1 145 33 33 SER H H 8.06 0.02 1 146 33 33 SER C C 174.457 0.80 1 147 33 33 SER CA C 58.34 0.80 1 148 33 33 SER CB C 63.605 0.80 1 149 33 33 SER N N 114.871 0.25 1 150 34 34 ALA H H 8.186 0.02 1 151 34 34 ALA C C 177.793 0.80 1 152 34 34 ALA CA C 52.515 0.80 1 153 34 34 ALA CB C 18.905 0.80 1 154 34 34 ALA N N 126.062 0.25 1 155 35 35 SER H H 8.137 0.02 1 156 35 35 SER CA C 58.451 0.80 1 157 35 35 SER CB C 63.612 0.80 1 158 35 35 SER N N 114.769 0.25 1 159 36 36 GLY H H 8.233 0.02 1 160 36 36 GLY C C 174.276 0.80 1 161 36 36 GLY CA C 45.153 0.80 1 162 36 36 GLY N N 110.911 0.25 1 163 37 37 SER H H 8.162 0.02 1 164 37 37 SER C C 175.054 0.80 1 165 37 37 SER CA C 58.471 0.80 1 166 37 37 SER CB C 63.662 0.80 1 167 37 37 SER N N 115.678 0.25 1 168 38 38 GLY H H 8.354 0.02 1 169 38 38 GLY C C 173.842 0.80 1 170 38 38 GLY CA C 45.071 0.80 1 171 38 38 GLY N N 111.015 0.25 1 172 39 39 ALA H H 7.997 0.02 1 173 39 39 ALA C C 177.642 0.80 1 174 39 39 ALA CA C 52.362 0.80 1 175 39 39 ALA CB C 18.985 0.80 1 176 39 39 ALA N N 123.592 0.25 1 177 40 40 GLN H H 8.245 0.02 1 178 40 40 GLN C C 175.707 0.80 1 179 40 40 GLN CA C 55.329 0.80 1 180 40 40 GLN CB C 29.156 0.80 1 181 40 40 GLN N N 119.389 0.25 1 182 41 41 SER H H 8.246 0.02 1 183 41 41 SER C C 172.608 0.80 1 184 41 41 SER CA C 56.411 0.80 1 185 41 41 SER CB C 62.993 0.80 1 186 41 41 SER N N 118.842 0.25 1 187 42 42 PRO C C 176.924 0.80 1 188 42 42 PRO CA C 63.229 0.80 1 189 42 42 PRO CB C 31.912 0.80 1 190 43 43 GLN H H 8.348 0.02 1 191 43 43 GLN C C 175.707 0.80 1 192 43 43 GLN CA C 55.967 0.80 1 193 43 43 GLN CB C 29.01 0.80 1 194 43 43 GLN N N 120.092 0.25 1 195 44 44 ASN H H 8.228 0.02 1 196 44 44 ASN C C 174.708 0.80 1 197 44 44 ASN CA C 53.082 0.80 1 198 44 44 ASN CB C 38.595 0.80 1 199 44 44 ASN N N 119.658 0.25 1 200 45 45 ALA H H 8.134 0.02 1 201 45 45 ALA CA C 52.477 0.80 1 202 45 45 ALA CB C 18.95 0.80 1 203 45 45 ALA N N 124.398 0.25 1 204 46 46 SER H H 8.169 0.02 1 205 46 46 SER CA C 58.342 0.80 1 206 46 46 SER CB C 63.551 0.80 1 207 46 46 SER N N 115.12 0.25 1 208 47 47 ILE H H 7.949 0.02 1 209 47 47 ILE CA C 61.192 0.80 1 210 47 47 ILE CB C 38.426 0.80 1 211 47 47 ILE N N 122.336 0.25 1 212 48 48 MET H H 7.817 0.02 1 213 48 48 MET CA C 56.804 0.80 1 214 48 48 MET CB C 33.371 0.80 1 215 48 48 MET N N 129.302 0.25 1 stop_ save_