data_30184 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of parallel stranded adenosine duplex r(AAAA)-dA-r(AAA) ; _BMRB_accession_number 30184 _BMRB_flat_file_name bmr30184.str _Entry_type original _Submission_date 2016-09-27 _Accession_date 2016-09-27 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Denisov A. . . 2 Noronha A. . . 3 Gehring K. . . 4 Wilds C. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 96 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2017-12-12 update BMRB 'update entry citation' 2017-08-04 original author 'original release' stop_ _Original_release_date 2016-11-18 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Influence of nucleotide modifications at the C2' position on the Hoogsteen base-paired parallel-stranded duplex of poly(A) RNA. ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 28973475 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Copp William . . 2 Denisov Alexey Y. . 3 Xie Jingwei . . 4 Noronha Anne M. . 5 Liczner Christopher . . 6 Safaee Nozhat . . 7 Wilds Christopher J. . 8 Gehring Kalle . . stop_ _Journal_abbreviation 'Nucleic Acids Res.' _Journal_name_full 'Nucleic acids research' _Journal_volume 45 _Journal_issue 17 _Journal_ISSN 1362-4962 _Journal_CSD 0353 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 10321 _Page_last 10331 _Year 2017 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name RNA _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'entity_1, chain 1' $entity_1 'entity_1, chain 2' $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class "DNA/RNA hybrid" _Name_common "RNA/DNA (5'-D(P*T)-R(*(A2M)P*(A2M)P*(A2M)P*(A2M))-D(P*A)-R(P*(A2M)P*(A2M)P*(A2M))-3')" _Molecular_mass 2975.072 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 9 _Mol_residue_sequence ; XXXXXXXXX ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 DT 2 2 A2M 3 3 A2M 4 4 A2M 5 5 A2M 6 6 DA 7 7 A2M 8 8 A2M 9 9 A2M stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ ###################### # Polymer residues # ###################### save_chem_comp_DT _Saveframe_category polymer_residue _Mol_type 'DNA LINKING' _Name_common THYMIDINE-5'-MONOPHOSPHATE _BMRB_code DT _PDB_code DT _Standard_residue_derivative . _Molecular_mass 322.208 _Mol_paramagnetic . _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons OP3 OP3 O . 0 . ? P P P . 0 . ? OP1 OP1 O . 0 . ? OP2 OP2 O . 0 . ? O5' O5' O . 0 . ? C5' C5' C . 0 . ? C4' C4' C . 0 . ? O4' O4' O . 0 . ? C3' C3' C . 0 . ? O3' O3' O . 0 . ? C2' C2' C . 0 . ? C1' C1' C . 0 . ? N1 N1 N . 0 . ? C2 C2 C . 0 . ? O2 O2 O . 0 . ? N3 N3 N . 0 . ? C4 C4 C . 0 . ? O4 O4 O . 0 . ? C5 C5 C . 0 . ? C7 C7 C . 0 . ? C6 C6 C . 0 . ? HOP3 HOP3 H . 0 . ? HOP2 HOP2 H . 0 . ? H5' H5' H . 0 . ? H5'' H5'' H . 0 . ? H4' H4' H . 0 . ? H3' H3' H . 0 . ? HO3' HO3' H . 0 . ? H2' H2' H . 0 . ? H2'' H2'' H . 0 . ? H1' H1' H . 0 . ? H3 H3 H . 0 . ? H71 H71 H . 0 . ? H72 H72 H . 0 . ? H73 H73 H . 0 . ? H6 H6 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name SING OP3 P ? ? SING OP3 HOP3 ? ? DOUB P OP1 ? ? SING P OP2 ? ? SING P O5' ? ? SING OP2 HOP2 ? ? SING O5' C5' ? ? SING C5' C4' ? ? SING C5' H5' ? ? SING C5' H5'' ? ? SING C4' O4' ? ? SING C4' C3' ? ? SING C4' H4' ? ? SING O4' C1' ? ? SING C3' O3' ? ? SING C3' C2' ? ? SING C3' H3' ? ? SING O3' HO3' ? ? SING C2' C1' ? ? SING C2' H2' ? ? SING C2' H2'' ? ? SING C1' N1 ? ? SING C1' H1' ? ? SING N1 C2 ? ? SING N1 C6 ? ? DOUB C2 O2 ? ? SING C2 N3 ? ? SING N3 C4 ? ? SING N3 H3 ? ? DOUB C4 O4 ? ? SING C4 C5 ? ? SING C5 C7 ? ? DOUB C5 C6 ? ? SING C7 H71 ? ? SING C7 H72 ? ? SING C7 H73 ? ? SING C6 H6 ? ? stop_ save_ save_chem_comp_A2M _Saveframe_category polymer_residue _Mol_type 'RNA LINKING' _Name_common "2'-O-methyladenosine 5'-(dihydrogen phosphate)" _BMRB_code A2M _PDB_code A2M _Standard_residue_derivative . _Molecular_mass 361.248 _Mol_paramagnetic . _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons P P P . 0 . ? OP1 OP1 O . 0 . ? OP3 OP3 O . 0 . ? O5' O5' O . 0 . ? C5' C5' C . 0 . ? C4' C4' C . 0 . ? O4' O4' O . 0 . ? C3' C3' C . 0 . ? O3' O3' O . 0 . ? C2' C2' C . 0 . ? O2' O2' O . 0 . ? C1' C1' C . 0 . ? CM' CM' C . 0 . ? N9 N9 N . 0 . ? C8 C8 C . 0 . ? N7 N7 N . 0 . ? C5 C5 C . 0 . ? C6 C6 C . 0 . ? N6 N6 N . 0 . ? N1 N1 N . 0 . ? C2 C2 C . 0 . ? N3 N3 N . 0 . ? C4 C4 C . 0 . ? HOP3 HOP3 H . 0 . ? H5' H5' H . 0 . ? H5'' H5'' H . 0 . ? H4' H4' H . 0 . ? H3' H3' H . 0 . ? HO3' HO3' H . 0 . ? H2' H2' H . 0 . ? H1' H1' H . 0 . ? HM'1 HM'1 H . 0 . ? HM'2 HM'2 H . 0 . ? HM'3 HM'3 H . 0 . ? H8 H8 H . 0 . ? H61 H61 H . 0 . ? H62 H62 H . 0 . ? H2 H2 H . 0 . ? OP2 OP2 O . 0 . ? HOP2 HOP2 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name DOUB P OP1 ? ? SING P OP3 ? ? SING P O5' ? ? SING OP3 HOP3 ? ? SING O5' C5' ? ? SING C5' C4' ? ? SING C5' H5' ? ? SING C5' H5'' ? ? SING C4' O4' ? ? SING C4' C3' ? ? SING C4' H4' ? ? SING O4' C1' ? ? SING C3' O3' ? ? SING C3' C2' ? ? SING C3' H3' ? ? SING O3' HO3' ? ? SING C2' O2' ? ? SING C2' C1' ? ? SING C2' H2' ? ? SING O2' CM' ? ? SING C1' N9 ? ? SING C1' H1' ? ? SING CM' HM'1 ? ? SING CM' HM'2 ? ? SING CM' HM'3 ? ? SING N9 C8 ? ? SING N9 C4 ? ? DOUB C8 N7 ? ? SING C8 H8 ? ? SING N7 C5 ? ? SING C5 C6 ? ? DOUB C5 C4 ? ? SING C6 N6 ? ? DOUB C6 N1 ? ? SING N6 H61 ? ? SING N6 H62 ? ? SING N1 C2 ? ? DOUB C2 N3 ? ? SING C2 H2 ? ? SING N3 C4 ? ? SING P OP2 ? ? SING OP2 HOP2 ? ? stop_ save_ save_chem_comp_DA _Saveframe_category polymer_residue _Mol_type 'DNA LINKING' _Name_common 2'-DEOXYADENOSINE-5'-MONOPHOSPHATE _BMRB_code DA _PDB_code DA _Standard_residue_derivative . _Molecular_mass 331.222 _Mol_paramagnetic . _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons OP3 OP3 O . 0 . ? P P P . 0 . ? OP1 OP1 O . 0 . ? OP2 OP2 O . 0 . ? O5' O5' O . 0 . ? C5' C5' C . 0 . ? C4' C4' C . 0 . ? O4' O4' O . 0 . ? C3' C3' C . 0 . ? O3' O3' O . 0 . ? C2' C2' C . 0 . ? C1' C1' C . 0 . ? N9 N9 N . 0 . ? C8 C8 C . 0 . ? N7 N7 N . 0 . ? C5 C5 C . 0 . ? C6 C6 C . 0 . ? N6 N6 N . 0 . ? N1 N1 N . 0 . ? C2 C2 C . 0 . ? N3 N3 N . 0 . ? C4 C4 C . 0 . ? HOP3 HOP3 H . 0 . ? HOP2 HOP2 H . 0 . ? H5' H5' H . 0 . ? H5'' H5'' H . 0 . ? H4' H4' H . 0 . ? H3' H3' H . 0 . ? HO3' HO3' H . 0 . ? H2' H2' H . 0 . ? H2'' H2'' H . 0 . ? H1' H1' H . 0 . ? H8 H8 H . 0 . ? H61 H61 H . 0 . ? H62 H62 H . 0 . ? H2 H2 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name SING OP3 P ? ? SING OP3 HOP3 ? ? DOUB P OP1 ? ? SING P OP2 ? ? SING P O5' ? ? SING OP2 HOP2 ? ? SING O5' C5' ? ? SING C5' C4' ? ? SING C5' H5' ? ? SING C5' H5'' ? ? SING C4' O4' ? ? SING C4' C3' ? ? SING C4' H4' ? ? SING O4' C1' ? ? SING C3' O3' ? ? SING C3' C2' ? ? SING C3' H3' ? ? SING O3' HO3' ? ? SING C2' C1' ? ? SING C2' H2' ? ? SING C2' H2'' ? ? SING C1' N9 ? ? SING C1' H1' ? ? SING N9 C8 ? ? SING N9 C4 ? ? DOUB C8 N7 ? ? SING C8 H8 ? ? SING N7 C5 ? ? SING C5 C6 ? ? DOUB C5 C4 ? ? SING C6 N6 ? ? DOUB C6 N1 ? ? SING N6 H61 ? ? SING N6 H62 ? ? SING N1 C2 ? ? DOUB C2 N3 ? ? SING C2 H2 ? ? SING N3 C4 ? ? stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '1.0 mM TA8 duplex, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1.0 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '1.0 mM TA8 duplex, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1.0 mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name CNS _Version 1.2 loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_software_2 _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_3 _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_software_4 _Saveframe_category software _Name VNMR _Version . loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model UNITY _Field_strength 800 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model VNMRS _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_DQF-COSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D DQF-COSY' _Sample_label $sample_2 save_ save_2D_1H-1H_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_2 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_31P-1H_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 31P-1H HSQC' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 50 . mM pH 4.0 . pH pressure 1 . atm temperature 283 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 50 . mM pH 4.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0 na direct . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D DQF-COSY' '2D 1H-1H TOCSY' '2D 31P-1H HSQC' stop_ loop_ _Sample_label $sample_1 $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name 'entity_1, chain 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 DT H1' H 5.62 0.01 1 2 1 1 DT H2' H 2.41 0.01 1 3 1 1 DT H2'' H 2.53 0.01 1 4 1 1 DT H3 H 10.49 0.01 1 5 1 1 DT H3' H 4.52 0.01 1 6 1 1 DT H4' H 4.05 0.01 1 7 1 1 DT H6 H 7.59 0.01 1 8 1 1 DT H71 H 1.30 0.01 1 9 1 1 DT H72 H 1.30 0.01 1 10 1 1 DT H73 H 1.30 0.01 1 11 2 2 A2M H1' H 6.26 0.01 1 12 2 2 A2M H2 H 7.80 0.01 1 13 2 2 A2M H2' H 4.35 0.01 1 14 2 2 A2M H3' H 4.58 0.01 1 15 2 2 A2M H4' H 4.44 0.01 1 16 2 2 A2M H8 H 8.39 0.01 1 17 2 2 A2M H61 H 8.85 0.01 1 18 2 2 A2M H62 H 7.43 0.01 1 19 2 2 A2M HM'1 H 3.79 0.01 1 20 2 2 A2M HM'2 H 3.79 0.01 1 21 2 2 A2M HM'3 H 3.79 0.01 1 22 3 3 A2M H1' H 5.75 0.01 1 23 3 3 A2M H2 H 8.33 0.01 1 24 3 3 A2M H2' H 4.26 0.01 1 25 3 3 A2M H3' H 4.09 0.01 1 26 3 3 A2M H4' H 4.50 0.01 1 27 3 3 A2M H8 H 7.71 0.01 1 28 3 3 A2M H61 H 9.49 0.01 1 29 3 3 A2M H62 H 8.09 0.01 1 30 3 3 A2M HM'1 H 3.80 0.01 1 31 3 3 A2M HM'2 H 3.80 0.01 1 32 3 3 A2M HM'3 H 3.80 0.01 1 33 4 4 A2M H1' H 6.06 0.01 1 34 4 4 A2M H2 H 8.32 0.01 1 35 4 4 A2M H2' H 4.16 0.01 1 36 4 4 A2M H3' H 4.09 0.01 1 37 4 4 A2M H4' H 4.48 0.01 1 38 4 4 A2M H8 H 7.41 0.01 1 39 4 4 A2M H61 H 9.02 0.01 1 40 4 4 A2M H62 H 7.68 0.01 1 41 4 4 A2M HM'1 H 3.82 0.01 1 42 4 4 A2M HM'2 H 3.82 0.01 1 43 4 4 A2M HM'3 H 3.82 0.01 1 44 5 5 A2M H1' H 6.11 0.01 1 45 5 5 A2M H2 H 8.33 0.01 1 46 5 5 A2M H2' H 4.22 0.01 1 47 5 5 A2M H3' H 4.05 0.01 1 48 5 5 A2M H4' H 4.47 0.01 1 49 5 5 A2M H8 H 7.34 0.01 1 50 5 5 A2M H61 H 9.15 0.01 1 51 5 5 A2M H62 H 8.07 0.01 1 52 5 5 A2M HM'1 H 3.83 0.01 1 53 5 5 A2M HM'2 H 3.83 0.01 1 54 5 5 A2M HM'3 H 3.83 0.01 1 55 6 6 DA H1' H 6.22 0.01 1 56 6 6 DA H2 H 8.43 0.01 1 57 6 6 DA H2' H 2.75 0.01 1 58 6 6 DA H2'' H 2.80 0.01 1 59 6 6 DA H3' H 4.09 0.01 1 60 6 6 DA H4' H 4.28 0.01 1 61 6 6 DA H8 H 7.27 0.01 1 62 6 6 DA H61 H 9.30 0.01 1 63 6 6 DA H62 H 8.32 0.01 1 64 7 7 A2M H1' H 6.20 0.01 1 65 7 7 A2M H2 H 8.28 0.01 1 66 7 7 A2M H2' H 4.29 0.01 1 67 7 7 A2M H3' H 4.23 0.01 1 68 7 7 A2M H4' H 4.48 0.01 1 69 7 7 A2M H8 H 7.30 0.01 1 70 7 7 A2M H61 H 9.25 0.01 1 71 7 7 A2M H62 H 8.29 0.01 1 72 7 7 A2M HM'1 H 3.90 0.01 1 73 7 7 A2M HM'2 H 3.90 0.01 1 74 7 7 A2M HM'3 H 3.90 0.01 1 75 8 8 A2M H1' H 6.30 0.01 1 76 8 8 A2M H2 H 8.26 0.01 1 77 8 8 A2M H2' H 4.27 0.01 1 78 8 8 A2M H3' H 4.35 0.01 1 79 8 8 A2M H4' H 4.61 0.01 1 80 8 8 A2M H8 H 7.49 0.01 1 81 8 8 A2M H61 H 9.31 0.01 1 82 8 8 A2M H62 H 8.43 0.01 1 83 8 8 A2M HM'1 H 3.87 0.01 1 84 8 8 A2M HM'2 H 3.87 0.01 1 85 8 8 A2M HM'3 H 3.87 0.01 1 86 9 9 A2M H1' H 6.22 0.01 1 87 9 9 A2M H2 H 8.41 0.01 1 88 9 9 A2M H2' H 3.72 0.01 1 89 9 9 A2M H3' H 4.02 0.01 1 90 9 9 A2M H4' H 4.27 0.01 1 91 9 9 A2M H8 H 7.66 0.01 1 92 9 9 A2M H61 H 9.32 0.01 1 93 9 9 A2M H62 H 8.02 0.01 1 94 9 9 A2M HM'1 H 3.63 0.01 1 95 9 9 A2M HM'2 H 3.63 0.01 1 96 9 9 A2M HM'3 H 3.63 0.01 1 stop_ save_