data_30262 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; HnRNP A1 Alters the Conformation of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation ; _BMRB_accession_number 30262 _BMRB_flat_file_name bmr30262.str _Entry_type original _Submission_date 2017-03-01 _Accession_date 2017-03-01 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Tolbert M. . . 2 Morgan C. E. . 3 Tolbert B. S. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 110 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2017-11-22 update BMRB 'update entry citation' 2017-07-07 original author 'original release' stop_ _Original_release_date 2017-07-06 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; HnRNP A1 Alters the Structure of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation. ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 28625847 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Tolbert M. . . 2 Morgan C. E. . 3 Pollum M. . . 4 Crespo-Hernandez C. E. . 5 Li M. L. . 6 Brewer G. . . 7 Tolbert B. S. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 429 _Journal_issue 19 _Journal_ASTM JMOBAK _Journal_ISSN 1089-8638 _Journal_CSD 0070 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2841 _Page_last 2858 _Year 2017 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'RNA (41-MER)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common entity_1 _Molecular_mass 13075.822 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 41 _Mol_residue_sequence ; GGAUCAACCCCAGGUGUGGC ACACCAGUCAUACCUUGAUC C ; loop_ _Residue_seq_code _Residue_label 1 G 2 G 3 A 4 U 5 C 6 A 7 A 8 C 9 C 10 C 11 C 12 A 13 G 14 G 15 U 16 G 17 U 18 G 19 G 20 C 21 A 22 C 23 A 24 C 25 C 26 A 27 G 28 U 29 C 30 A 31 U 32 A 33 C 34 C 35 U 36 U 37 G 38 A 39 U 40 C 41 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 'Enterovirus A71' 39054 Viruses . Enterovirus A71 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '220 uM Fully protonated rNTP, 100% D20' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 220 uM 'Fully protonated' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '300 uM Fully protonated rNTP, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 300 uM 'Fully protonated' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name AMBER _Version . loop_ _Vendor _Address _Electronic_address 'Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_software_2 _Saveframe_category software _Name NMRDraw _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_3 _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_4 _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_software_5 _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_software_6 _Saveframe_category software _Name 'X-PLOR NIH' _Version . loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task 'structure calculation' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 900 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 10 . mM pH 6.5 . pH pressure 1 . . temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.000 internal indirect . . . 0.25144953 DSS H 1 'methyl protons' ppm 0.000 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-1H TOCSY' stop_ loop_ _Sample_label $sample_1 $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 G H1' H 5.78306 . . 2 1 1 G H8 H 8.09749 . . 3 2 2 G H1' H 5.86916 . . 4 2 2 G H2' H 4.91083 . . 5 2 2 G H8 H 7.55435 . . 6 3 3 A H1' H 5.96046 . . 7 3 3 A H2 H 7.71792 . . 8 3 3 A H8 H 7.75835 . . 9 4 4 U H1' H 5.43714 . . 10 4 4 U H5 H 4.97534 . . 11 4 4 U H6 H 7.50715 . . 12 5 5 C H1' H 5.50082 . . 13 5 5 C H5 H 5.5282 . . 14 5 5 C H6 H 7.69332 . . 15 6 6 A H1' H 5.74438 . . 16 6 6 A H2 H 6.85076 . . 17 6 6 A H8 H 7.8475 . . 18 7 7 A H1' H 5.48474 . . 19 7 7 A H2 H 7.61829 . . 20 7 7 A H8 H 7.40264 . . 21 8 8 C H1' H 5.31679 . . 22 8 8 C H5 H 5.15544 . . 23 8 8 C H6 H 7.24031 . . 24 9 9 C H1' H 5.57638 . . 25 9 9 C H5 H 5.67266 . . 26 9 9 C H6 H 7.59917 . . 27 10 10 C H5 H 5.46037 . . 28 10 10 C H6 H 7.60906 . . 29 11 11 C H1' H 5.58128 . . 30 11 11 C H5 H 5.59437 . . 31 11 11 C H6 H 7.62979 . . 32 12 12 A H1' H 5.88809 . . 33 12 12 A H2 H 7.79286 . . 34 12 12 A H8 H 8.215 . . 35 13 13 G H1' H 5.68433 . . 36 13 13 G H8 H 7.3128 . . 37 14 14 G H1' H 5.78008 . . 38 14 14 G H8 H 7.18711 . . 39 15 15 U H1' H 5.6296 . . 40 15 15 U H5 H 5.13142 . . 41 15 15 U H6 H 7.61039 . . 42 16 16 G H1' H 5.74606 . . 43 16 16 G H8 H 7.69302 . . 44 17 17 U H1' H 5.41042 . . 45 17 17 U H6 H 7.60515 . . 46 18 18 G H1' H 5.77563 . . 47 18 18 G H8 H 7.677 . . 48 19 19 G H1' H 5.66206 . . 49 19 19 G H8 H 7.15017 . . 50 20 20 C H1' H 5.40958 . . 51 20 20 C H5 H 4.97534 . . 52 20 20 C H6 H 7.34492 . . 53 21 21 A H1' H 5.83353 . . 54 21 21 A H2 H 8.1333 . . 55 21 21 A H8 H 7.96549 . . 56 22 22 C H1' H 5.34834 . . 57 22 22 C H5 H 5.3706 . . 58 22 22 C H6 H 7.38427 . . 59 23 23 A H1' H 5.85468 . . 60 23 23 A H2 H 7.99199 . . 61 23 23 A H8 H 8.26038 . . 62 24 24 C H1' H 5.30798 . . 63 24 24 C H5 H 5.24151 . . 64 24 24 C H6 H 7.40672 . . 65 25 25 C H1' H 5.54323 . . 66 25 25 C H5 H 5.58766 . . 67 25 25 C H6 H 7.68191 . . 68 26 26 A H1' H 5.94264 . . 69 26 26 A H2 H 7.48949 . . 70 26 26 A H8 H 8.20497 . . 71 27 27 G H1' H 5.42267 . . 72 27 27 G H8 H 7.5329 . . 73 28 28 U H1' H 5.4061 . . 74 28 28 U H5 H 5.30111 . . 75 28 28 U H6 H 7.68944 . . 76 29 29 C H1' H 5.58332 . . 77 29 29 C H5 H 5.64634 . . 78 29 29 C H6 H 7.87301 . . 79 30 30 A H1' H 5.78565 . . 80 30 30 A H2 H 7.27368 . . 81 30 30 A H8 H 7.91399 . . 82 31 31 U H1' H 5.23366 . . 83 31 31 U H5 H 5.31822 . . 84 31 31 U H6 H 7.52088 . . 85 32 32 A H1' H 5.90146 . . 86 32 32 A H2 H 7.39043 . . 87 32 32 A H8 H 8.32022 . . 88 33 33 C H1' H 5.38509 . . 89 33 33 C H5 H 5.18356 . . 90 33 33 C H6 H 7.47281 . . 91 34 34 C H1' H 5.51706 . . 92 34 34 C H5 H 5.41736 . . 93 34 34 C H6 H 7.73765 . . 94 35 35 U H1' H 5.72098 . . 95 35 35 U H6 H 7.61799 . . 96 36 36 U H1' H 5.72331 . . 97 36 36 U H5 H 5.85456 . . 98 36 36 U H6 H 7.90027 . . 99 37 37 G H1' H 5.75339 . . 100 37 37 G H8 H 7.84807 . . 101 38 38 A H1' H 5.90256 . . 102 38 38 A H2 H 7.66043 . . 103 38 38 A H8 H 7.8063 . . 104 39 39 U H1' H 5.43565 . . 105 39 39 U H5 H 4.96687 . . 106 39 39 U H6 H 7.57504 . . 107 40 40 C H1' H 5.53757 . . 108 40 40 C H6 H 7.79297 . . 109 41 41 C H1' H 5.57141 . . 110 41 41 C H6 H 7.60878 . . stop_ save_