data_30552 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; MYC Promoter G-Quadruplex with 1:6:1 loop length ; _BMRB_accession_number 30552 _BMRB_flat_file_name bmr30552.str _Entry_type original _Submission_date 2018-12-17 _Accession_date 2018-12-17 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Dickerhoff J. . . 2 Onel B. . . 3 Chen L. . . 4 Chen Y. . . 5 Yang D. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 260 "13C chemical shifts" 32 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-02-12 update author 'update sample components' 2019-02-08 original author 'original release' stop_ _Original_release_date 2019-02-06 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Solution Structure of a MYC Promoter G-Quadruplex with 1:6:1 Loop Length ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Dickerhoff J. . . 2 Onel B. . . 3 Chen L. . . 4 Chen Y. . . 5 Yang D. . . stop_ _Journal_abbreviation 'Acs Omega' _Journal_volume 4 _Journal_issue 2 _Journal_ISSN 2470-1343 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2533 _Page_last 2539 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'DNA (27-MER)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common entity_1 _Molecular_mass 8563.501 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 27 _Mol_residue_sequence ; TTGGGGAGGGTTTTAAGGGT GGGGAAT ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 0 DT 2 1 DT 3 2 DG 4 3 DG 5 4 DG 6 5 DG 7 6 DA 8 7 DG 9 8 DG 10 9 DG 11 10 DT 12 11 DT 13 12 DT 14 13 DT 15 14 DA 16 15 DA 17 16 DG 18 17 DG 19 18 DG 20 19 DT 21 20 DG 22 21 DG 23 22 DG 24 23 DG 25 24 DA 26 25 DA 27 26 DT stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '1.5 mM DNA (27-MER), 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1.5 mM 'natural abundance' 'potassium phosphate' 25 mM 'natural abundance' 'potassium chloride' 75 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name AMBER _Version 16 loop_ _Vendor _Address _Electronic_address 'Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_software_2 _Saveframe_category software _Name 'X-PLOR NIH' _Version 2.48 loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement 'structure calculation' stop_ _Details . save_ save_software_3 _Saveframe_category software _Name CcpNMR _Version . loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ save_software_4 _Saveframe_category software _Name TOPSPIN _Version 3.5 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AvanceIII _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_aromatic_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aromatic' _Sample_label $sample_1 save_ save_2D_DQF-COSY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D DQF-COSY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 100 . mM pH 7 . pH pressure 1 . atm temperature 298 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 100 . mM pH 7 . pH pressure 1 . atm temperature 278 . K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 100 . mM pH 7 . pH pressure 1 . atm temperature 313 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.000 internal indirect . . . 0.25144953 water H 1 protons ppm 4.78 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-13C HSQC aromatic' '2D DQF-COSY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 0 1 DT H1' H 5.889 0.002 1 2 0 1 DT H2' H 2.031 0.003 1 3 0 1 DT H2'' H 2.277 0.002 1 4 0 1 DT H3' H 4.498 0.002 1 5 0 1 DT H4' H 3.909 0.001 1 6 0 1 DT H5' H 3.558 0.003 2 7 0 1 DT H5'' H 3.599 0.004 2 8 0 1 DT H6 H 7.356 0.001 1 9 0 1 DT H71 H 1.640 0.002 1 10 0 1 DT H72 H 1.640 0.002 1 11 0 1 DT H73 H 1.640 0.002 1 12 0 1 DT C6 C 139.802 . 1 13 1 2 DT H1' H 5.864 0.002 1 14 1 2 DT H2' H 1.848 0.002 1 15 1 2 DT H2'' H 2.170 0.003 1 16 1 2 DT H3' H 4.591 0.002 1 17 1 2 DT H4' H 3.849 0.001 1 18 1 2 DT H5' H 3.706 0.004 2 19 1 2 DT H5'' H 3.565 0.002 2 20 1 2 DT H6 H 7.253 0.002 1 21 1 2 DT H71 H 1.606 0.003 1 22 1 2 DT H72 H 1.606 0.003 1 23 1 2 DT H73 H 1.606 0.003 1 24 1 2 DT C6 C 139.581 . 1 25 2 3 DG H1' H 5.752 0.002 1 26 2 3 DG H2' H 2.712 0.004 1 27 2 3 DG H2'' H 2.661 0.002 1 28 2 3 DG H3' H 4.857 0.002 1 29 2 3 DG H4' H 4.242 0.003 1 30 2 3 DG H5' H 3.917 . 2 31 2 3 DG H5'' H 3.952 0.005 2 32 2 3 DG H8 H 7.645 0.002 1 33 2 3 DG C8 C 140.112 . 1 34 3 4 DG H1 H 11.861 0.001 1 35 3 4 DG H1' H 6.050 0.002 1 36 3 4 DG H2' H 2.744 0.004 1 37 3 4 DG H2'' H 2.976 0.001 1 38 3 4 DG H3' H 5.036 0.002 1 39 3 4 DG H4' H 4.470 0.001 1 40 3 4 DG H5' H 4.163 . 1 41 3 4 DG H5'' H 4.163 . 1 42 3 4 DG H8 H 8.044 0.002 1 43 3 4 DG C8 C 138.711 . 1 44 4 5 DG H1 H 12.168 2.307 1 45 4 5 DG H1' H 6.155 0.001 1 46 4 5 DG H2' H 2.608 0.002 1 47 4 5 DG H2'' H 2.887 0.003 1 48 4 5 DG H3' H 5.014 0.003 1 49 4 5 DG H4' H 4.555 0.001 1 50 4 5 DG H5' H 4.318 . 2 51 4 5 DG H5'' H 4.289 . 2 52 4 5 DG H8 H 7.692 0.002 1 53 4 5 DG H21 H 9.199 0.006 1 54 4 5 DG H22 H 6.809 0.003 1 55 4 5 DG C8 C 137.846 . 1 56 5 6 DG H1 H 12.244 2.556 1 57 5 6 DG H1' H 6.417 0.001 1 58 5 6 DG H2' H 2.686 0.002 1 59 5 6 DG H2'' H 2.606 0.001 1 60 5 6 DG H3' H 5.138 0.002 1 61 5 6 DG H4' H 4.645 0.002 1 62 5 6 DG H5' H 4.320 0.003 2 63 5 6 DG H5'' H 4.387 0.001 2 64 5 6 DG H8 H 7.612 0.002 1 65 5 6 DG C8 C 138.081 . 1 66 6 7 DA H1' H 6.694 0.002 1 67 6 7 DA H2 H 8.329 0.001 1 68 6 7 DA H2' H 2.923 0.003 1 69 6 7 DA H2'' H 2.933 0.006 1 70 6 7 DA H3' H 5.236 0.002 1 71 6 7 DA H4' H 4.730 0.002 1 72 6 7 DA H5' H 4.305 . 2 73 6 7 DA H5'' H 4.328 . 2 74 6 7 DA H8 H 8.553 0.003 1 75 6 7 DA C2 C 155.930 . 1 76 6 7 DA C8 C 142.842 . 1 77 7 8 DG H1 H 11.210 0.002 1 78 7 8 DG H1' H 6.061 0.001 1 79 7 8 DG H2' H 2.445 0.002 1 80 7 8 DG H2'' H 2.847 0.002 1 81 7 8 DG H3' H 5.148 0.002 1 82 7 8 DG H4' H 4.501 0.003 1 83 7 8 DG H5' H 4.411 0.003 2 84 7 8 DG H5'' H 4.327 0.001 2 85 7 8 DG H8 H 8.038 0.002 1 86 7 8 DG C8 C 138.444 . 1 87 8 9 DG H1 H 11.468 0.002 1 88 8 9 DG H1' H 6.034 0.003 1 89 8 9 DG H2' H 2.662 0.002 1 90 8 9 DG H2'' H 2.784 0.002 1 91 8 9 DG H3' H 5.048 0.002 1 92 8 9 DG H4' H 4.468 0.003 1 93 8 9 DG H5' H 4.199 0.002 2 94 8 9 DG H5'' H 4.264 0.001 2 95 8 9 DG H8 H 7.953 0.002 1 96 8 9 DG H21 H 9.358 0.001 1 97 8 9 DG C8 C 138.851 . 1 98 9 10 DG H1 H 11.289 0.001 1 99 9 10 DG H1' H 6.320 0.003 1 100 9 10 DG H2' H 2.596 0.002 1 101 9 10 DG H2'' H 2.566 0.005 1 102 9 10 DG H3' H 4.945 0.001 1 103 9 10 DG H4' H 4.284 0.002 1 104 9 10 DG H5' H 4.116 0.005 1 105 9 10 DG H5'' H 3.993 0.002 1 106 9 10 DG H8 H 7.803 0.001 1 107 9 10 DG C8 C 138.399 . 1 108 10 11 DT H1' H 6.219 0.003 1 109 10 11 DT H2' H 2.445 0.006 1 110 10 11 DT H2'' H 2.482 0.004 1 111 10 11 DT H3' H 4.843 0.002 1 112 10 11 DT H4' H 4.343 0.001 1 113 10 11 DT H5' H 4.158 0.002 2 114 10 11 DT H5'' H 4.048 0.002 2 115 10 11 DT H6 H 7.572 0.001 1 116 10 11 DT H71 H 1.933 0.002 1 117 10 11 DT H72 H 1.933 0.002 1 118 10 11 DT H73 H 1.933 0.002 1 119 10 11 DT C6 C 140.330 . 1 120 11 12 DT H1' H 6.303 0.002 1 121 11 12 DT H2' H 2.424 0.002 1 122 11 12 DT H2'' H 2.549 0.002 1 123 11 12 DT H3' H 4.938 0.002 1 124 11 12 DT H4' H 4.364 0.002 1 125 11 12 DT H5' H 4.124 0.004 2 126 11 12 DT H5'' H 4.177 0.004 2 127 11 12 DT H6 H 7.658 0.001 1 128 11 12 DT H71 H 1.923 0.001 1 129 11 12 DT H72 H 1.923 0.001 1 130 11 12 DT H73 H 1.923 0.001 1 131 11 12 DT C6 C 140.223 . 1 132 12 13 DT H1' H 6.314 0.002 1 133 12 13 DT H2' H 2.368 0.003 1 134 12 13 DT H2'' H 2.547 0.002 1 135 12 13 DT H3' H 4.905 0.003 1 136 12 13 DT H4' H 4.372 0.003 1 137 12 13 DT H5' H 4.184 0.001 2 138 12 13 DT H5'' H 4.125 0.001 2 139 12 13 DT H6 H 7.723 0.001 1 140 12 13 DT H71 H 1.949 0.002 1 141 12 13 DT H72 H 1.949 0.002 1 142 12 13 DT H73 H 1.949 0.002 1 143 12 13 DT C6 C 140.294 . 1 144 13 14 DT H1' H 6.031 0.002 1 145 13 14 DT H2' H 1.873 0.003 1 146 13 14 DT H2'' H 2.233 0.003 1 147 13 14 DT H3' H 4.765 0.003 1 148 13 14 DT H4' H 4.132 0.001 1 149 13 14 DT H5' H 3.986 0.001 1 150 13 14 DT H5'' H 3.986 0.001 1 151 13 14 DT H6 H 7.475 0.001 1 152 13 14 DT H71 H 1.907 0.003 1 153 13 14 DT H72 H 1.907 0.003 1 154 13 14 DT H73 H 1.907 0.003 1 155 13 14 DT C6 C 139.982 . 1 156 14 15 DA H1' H 6.029 . 1 157 14 15 DA H2 H 8.137 0.002 1 158 14 15 DA H2' H 2.895 0.001 1 159 14 15 DA H2'' H 2.758 0.001 1 160 14 15 DA H3' H 5.044 0.002 1 161 14 15 DA H4' H 4.438 0.001 1 162 14 15 DA H5' H 4.057 0.001 2 163 14 15 DA H5'' H 4.089 0.004 2 164 14 15 DA H8 H 8.382 0.001 1 165 14 15 DA C2 C 155.768 . 1 166 14 15 DA C8 C 142.829 . 1 167 15 16 DA H1' H 6.015 0.002 1 168 15 16 DA H2 H 7.922 0.002 1 169 15 16 DA H2' H 3.029 0.002 1 170 15 16 DA H2'' H 2.736 0.003 1 171 15 16 DA H3' H 5.063 0.002 1 172 15 16 DA H4' H 4.394 0.001 1 173 15 16 DA H5' H 4.098 0.003 2 174 15 16 DA H5'' H 4.098 0.003 2 175 15 16 DA H8 H 8.096 0.002 1 176 15 16 DA C2 C 154.862 . 1 177 15 16 DA C8 C 143.324 . 1 178 16 17 DG H1 H 11.986 0.002 1 179 16 17 DG H1' H 6.222 0.002 1 180 16 17 DG H2' H 2.849 0.003 1 181 16 17 DG H2'' H 3.071 0.002 1 182 16 17 DG H3' H 5.070 0.002 1 183 16 17 DG H4' H 4.512 0.004 1 184 16 17 DG H5' H 4.161 0.001 2 185 16 17 DG H5'' H 4.207 0.001 2 186 16 17 DG H8 H 8.218 0.002 1 187 16 17 DG C8 C 139.342 . 1 188 17 18 DG H1 H 11.342 0.003 1 189 17 18 DG H1' H 6.248 0.001 1 190 17 18 DG H2' H 2.733 0.003 1 191 17 18 DG H2'' H 3.006 0.001 1 192 17 18 DG H3' H 5.094 0.002 1 193 17 18 DG H4' H 4.608 0.001 1 194 17 18 DG H5' H 4.349 . 2 195 17 18 DG H5'' H 4.373 . 2 196 17 18 DG H8 H 7.796 0.002 1 197 17 18 DG H21 H 9.326 0.004 1 198 17 18 DG H22 H 6.867 0.005 1 199 17 18 DG C8 C 137.976 . 1 200 18 19 DG H1 H 11.231 0.003 1 201 18 19 DG H1' H 6.507 0.001 1 202 18 19 DG H2' H 2.727 0.002 1 203 18 19 DG H2'' H 2.645 0.001 1 204 18 19 DG H3' H 5.165 0.003 1 205 18 19 DG H4' H 4.674 0.002 1 206 18 19 DG H5' H 4.353 0.004 2 207 18 19 DG H5'' H 4.450 0.0 2 208 18 19 DG H8 H 7.835 0.002 1 209 18 19 DG C8 C 138.412 . 1 210 19 20 DT H1' H 6.559 0.002 1 211 19 20 DT H2' H 2.503 0.002 1 212 19 20 DT H2'' H 2.712 0.003 1 213 19 20 DT H3' H 5.159 0.001 1 214 19 20 DT H4' H 4.657 0.003 1 215 19 20 DT H5' H 4.344 0.001 2 216 19 20 DT H5'' H 4.391 0.0 2 217 19 20 DT H6 H 7.893 0.001 1 218 19 20 DT H71 H 2.018 0.004 1 219 19 20 DT H72 H 2.018 0.004 1 220 19 20 DT H73 H 2.018 0.004 1 221 19 20 DT C6 C 140.250 . 1 222 20 21 DG H1 H 11.472 0.002 1 223 20 21 DG H1' H 6.135 0.001 1 224 20 21 DG H2' H 2.451 0.003 1 225 20 21 DG H2'' H 2.895 0.002 1 226 20 21 DG H3' H 5.153 0.002 1 227 20 21 DG H4' H 4.468 0.004 1 228 20 21 DG H5' H 4.267 0.001 2 229 20 21 DG H5'' H 4.362 0.004 2 230 20 21 DG H8 H 8.022 0.002 1 231 20 21 DG C8 C 138.407 . 1 232 21 22 DG H1 H 11.352 0.003 1 233 21 22 DG H1' H 6.013 0.003 1 234 21 22 DG H2' H 2.658 0.001 1 235 21 22 DG H2'' H 2.739 0.001 1 236 21 22 DG H3' H 5.077 0.002 1 237 21 22 DG H4' H 4.498 0.0 1 238 21 22 DG H5' H 4.274 . 2 239 21 22 DG H5'' H 4.218 . 2 240 21 22 DG H8 H 7.963 0.001 1 241 21 22 DG C8 C 138.769 . 1 242 22 23 DG H1 H 10.903 0.003 1 243 22 23 DG H1' H 5.927 0.002 1 244 22 23 DG H2' H 2.112 0.002 1 245 22 23 DG H2'' H 2.530 0.003 1 246 22 23 DG H3' H 4.932 0.001 1 247 22 23 DG H4' H 4.467 0.003 1 248 22 23 DG H5' H 4.262 0.003 2 249 22 23 DG H5'' H 4.172 0.003 2 250 22 23 DG H8 H 7.374 0.002 1 251 22 23 DG C8 C 137.092 . 1 252 23 24 DG H1' H 5.143 0.003 1 253 23 24 DG H2' H 2.328 0.002 1 254 23 24 DG H2'' H 2.321 0.001 1 255 23 24 DG H3' H 4.854 0.001 1 256 23 24 DG H4' H 4.280 0.003 1 257 23 24 DG H5' H 4.282 . 2 258 23 24 DG H5'' H 4.113 . 2 259 23 24 DG H8 H 7.678 0.001 1 260 23 24 DG C8 C 138.637 . 1 261 24 25 DA H1' H 5.664 0.003 1 262 24 25 DA H2 H 7.515 0.002 1 263 24 25 DA H2' H 2.309 0.002 1 264 24 25 DA H2'' H 2.388 0.004 1 265 24 25 DA H3' H 4.752 0.002 1 266 24 25 DA H4' H 3.668 0.002 1 267 24 25 DA H5' H 3.879 0.003 2 268 24 25 DA H5'' H 3.840 0.014 2 269 24 25 DA H8 H 7.919 0.002 1 270 24 25 DA C2 C 155.124 . 1 271 24 25 DA C8 C 141.965 . 1 272 25 26 DA H1' H 5.890 0.001 1 273 25 26 DA H2 H 7.637 0.002 1 274 25 26 DA H2' H 2.505 0.002 1 275 25 26 DA H2'' H 2.545 0.003 1 276 25 26 DA H3' H 4.801 0.003 1 277 25 26 DA H4' H 4.236 0.003 1 278 25 26 DA H5' H 3.991 0.002 2 279 25 26 DA H5'' H 3.812 0.003 2 280 25 26 DA H8 H 7.964 0.001 1 281 25 26 DA C2 C 155.185 . 1 282 25 26 DA C8 C 141.459 . 1 283 26 27 DT H1' H 5.872 0.004 1 284 26 27 DT H2' H 2.103 0.001 1 285 26 27 DT H2'' H 2.145 0.003 1 286 26 27 DT H3' H 4.393 0.003 1 287 26 27 DT H4' H 3.940 0.004 1 288 26 27 DT H6 H 7.262 0.002 1 289 26 27 DT H71 H 1.540 0.003 1 290 26 27 DT H72 H 1.540 0.003 1 291 26 27 DT H73 H 1.540 0.003 1 292 26 27 DT C6 C 139.595 . 1 stop_ save_