data_34303 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Receptor-bound Ghrelin conformation ; _BMRB_accession_number 34303 _BMRB_flat_file_name bmr34303.str _Entry_type original _Submission_date 2018-07-18 _Accession_date 2018-07-18 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ferre G. . . 2 Damian M. . . 3 M'Kadmi C. . . 4 Saurel O. . . 5 Czaplicki G. . . 6 Demange P. . . 7 Marie J. . . 8 Fehrentz J. A. . 9 Baneres J. L. . 10 Milon A. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 133 "15N chemical shifts" 9 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-08-17 update BMRB 'update entry citation' 2019-07-15 original author 'original release' stop_ _Original_release_date 2018-07-20 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structure and dynamics of G protein-coupled receptor-bound ghrelin reveal the critical role of the octanoyl chain ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31416915 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ferre Guillaume . . 2 Louet Maxime . . 3 Saurel Oliver . . 4 Delort Bartholome . . 5 Czaplicki Georges . . 6 M'Kadmi Celine . . 7 Damian Marjorie . . 8 Renault Pedro . . 9 Cantel Sonia . . 10 Gavara Laurent . . 11 Demange Pascal . . 12 Marie Jacky . . 13 Fehrentz Jean-Alain A. . 14 Floquet Nicolas . . 15 Milon Alain . . 16 Baneres Jean-Louis L. . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_name_full 'Proceedings of the National Academy of Sciences of the United States of America' _Journal_volume 116 _Journal_issue 35 _Journal_ISSN 1091-6490 _Journal_CSD 0353 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 17525 _Page_last 17530 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Appetite-regulating hormone' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $entity_1 entity_2 $entity_FKZ stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass 2130.301 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 19 _Mol_residue_sequence ; GSDFLSPEHQRVQQRKESX ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 ASP 4 PHE 5 LEU 6 SER 7 PRO 8 GLU 9 HIS 10 GLN 11 ARG 12 VAL 13 GLN 14 GLN 15 ARG 16 LYS 17 GLU 18 SER 19 NH2 stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ ###################### # Polymer residues # ###################### save_chem_comp_NH2 _Saveframe_category polymer_residue _Mol_type NON-POLYMER _Name_common 'AMINO GROUP' _BMRB_code NH2 _PDB_code NH2 _Standard_residue_derivative . _Molecular_mass 16.023 _Mol_paramagnetic . _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons N N N . 0 . ? HN1 HN1 H . 0 . ? HN2 HN2 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name SING N HN1 ? ? SING N HN2 ? ? stop_ save_ ############# # Ligands # ############# save_FKZ _Saveframe_category ligand _Mol_type "non-polymer (NON-POLYMER)" _Name_common "entity_FKZ (octan-1-amine)" _BMRB_code FKZ _PDB_code FKZ _Molecular_mass 129.243 _Mol_charge 0 _Mol_paramagnetic . _Mol_aromatic no _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons C1 C1 C . 0 . ? C2 C2 C . 0 . ? C3 C3 C . 0 . ? C4 C4 C . 0 . ? C5 C5 C . 0 . ? C6 C6 C . 0 . ? C7 C7 C . 0 . ? C8 C8 C . 0 . ? N N N . 0 . ? H12 H12 H . 0 . ? H11 H11 H . 0 . ? H22 H22 H . 0 . ? H21 H21 H . 0 . ? H31 H31 H . 0 . ? H32 H32 H . 0 . ? H41 H41 H . 0 . ? H42 H42 H . 0 . ? H52 H52 H . 0 . ? H51 H51 H . 0 . ? H62 H62 H . 0 . ? H61 H61 H . 0 . ? H72 H72 H . 0 . ? H71 H71 H . 0 . ? H81 H81 H . 0 . ? H83 H83 H . 0 . ? H82 H82 H . 0 . ? H H H . 0 . ? H1 H1 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name SING C5 C4 ? ? SING C5 C6 ? ? SING C4 C3 ? ? SING C7 C6 ? ? SING C7 C8 ? ? SING C1 N ? ? SING C1 C2 ? ? SING C3 C2 ? ? SING C1 H12 ? ? SING C1 H11 ? ? SING C2 H22 ? ? SING C2 H21 ? ? SING C3 H31 ? ? SING C3 H32 ? ? SING C4 H41 ? ? SING C4 H42 ? ? SING C5 H52 ? ? SING C5 H51 ? ? SING C6 H62 ? ? SING C6 H61 ? ? SING C7 H72 ? ? SING C7 H71 ? ? SING C8 H81 ? ? SING C8 H83 ? ? SING C8 H82 ? ? SING N H ? ? SING N H1 ? ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '1.37 mM [U-15N]-Phe-Leu-Val Ghrelin 1-18, 2.05 uM [U-2H] Ghrelin receptor, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1.37 mM [U-15N]-Phe-Leu-Val 'Ghrelin receptor' 2.05 uM [U-2H] KCl 100 mM 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '1.37 mM [U-15N]-Phe-Leu-Val Ghrelin 1-18, 2.05 uM [U-2H] Ghrelin receptor, 2.05 uM JMV5327, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1.37 mM [U-15N]-Phe-Leu-Val 'Ghrelin receptor' 2.05 uM [U-2H] JMV5327 2.05 uM 'natural abundance' KCl 100 mM 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details '1.15 mM [U-15N]-Phe-Leu-Val-Ser-Gln-Glu Ghrelin 1-18, 1.72 uM [U-2H] Ghrelin receptor, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1.15 mM [U-15N]-Phe-Leu-Val-Ser-Gln-Glu 'Ghrelin receptor' 1.72 uM [U-2H] KCl 100 mM 'natural abundance' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details '1.15 mM [U-15N]-Phe-Leu-Val-Ser-Gln-Glu Ghrelin 1-18, 1.72 uM [U-2H] Ghrelin receptor, 1.72 uM JMV5327, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1.15 mM [U-15N]-Phe-Leu-Val-Ser-Gln-Glu 'Ghrelin receptor' 1.72 uM [U-2H] JMV5327 1.72 uM 'natural abundance' KCl 100 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name TOPSPIN _Version 3.5 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_software_2 _Saveframe_category software _Name TOPSPIN _Version 3.2 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_3 _Saveframe_category software _Name SPARKY _Version 1.2 loop_ _Vendor _Address _Electronic_address 'T. D. Goddard and D. G. Kneller, SPARKY 3, UCSF' . . stop_ loop_ _Task 'peak picking' stop_ _Details ; T. D. Goddard and D. G. Kneller, SPARKY 3, University of California, San Francisco ; save_ save_software_4 _Saveframe_category software _Name AMBER _Version 14 loop_ _Vendor _Address _Electronic_address 'Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman' . . stop_ loop_ _Task 'structure calculation' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AvanceIII _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_3D_1H-1H-15N_NOESY-HMQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-1H-15N NOESY-HMQC' _Sample_label $sample_3 save_ save_3D_1H-1H-15N_NOESY-HMQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-1H-15N NOESY-HMQC' _Sample_label $sample_4 save_ save_2D_1H-1H_TOCSY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_2 save_ save_2D_1H-1H_TOCSY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H-15N_SOFAST-HMQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N SOFAST-HMQC' _Sample_label $sample_3 save_ save_2D_1H-15N_SOFAST-HMQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N SOFAST-HMQC' _Sample_label $sample_4 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 100 . mM pH 6.5 0.1 pH pressure 1 . atm temperature 280 1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.000 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.000 internal indirect . . . 0.10132912 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '3D 1H-1H-15N NOESY-HMQC' '2D 1H-1H TOCSY' '2D 1H-15N SOFAST-HMQC' stop_ loop_ _Sample_label $sample_1 $sample_2 $sample_3 $sample_4 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 GLY HA2 H 3.884 0.01 1 2 1 1 GLY HA3 H 3.884 0.01 1 3 2 2 SER HA H 4.457 0.01 1 4 2 2 SER HB2 H 3.781 0.01 2 5 2 2 SER HB3 H 3.753 0.01 2 6 3 3 ASP H H 8.620 0.01 1 7 3 3 ASP HA H 4.680 0.01 1 8 3 3 ASP HB2 H 2.646 0.01 1 9 3 3 ASP HB3 H 2.731 0.01 1 10 4 4 PHE H H 8.329 0.01 1 11 4 4 PHE HA H 4.513 0.01 1 12 4 4 PHE HB2 H 3.108 0.01 2 13 4 4 PHE HB3 H 3.012 0.01 2 14 4 4 PHE HD1 H 7.212 0.01 3 15 4 4 PHE HD2 H 7.212 0.01 3 16 4 4 PHE HE1 H 7.344 0.01 3 17 4 4 PHE HE2 H 7.344 0.01 3 18 4 4 PHE HZ H 7.306 0.01 1 19 4 4 PHE N N 121.24 0.10 1 20 5 5 LEU H H 8.262 0.01 1 21 5 5 LEU HA H 4.349 0.01 1 22 5 5 LEU HB2 H 1.593 0.01 2 23 5 5 LEU HB3 H 1.490 0.01 2 24 5 5 LEU HG H 1.502 0.01 1 25 5 5 LEU HD1 H 0.809 0.01 1 26 5 5 LEU HD2 H 0.866 0.01 1 27 5 5 LEU N N 123.35 0.10 1 28 6 6 SER H H 8.337 0.01 1 29 6 6 SER HA H 4.656 0.01 1 30 6 6 SER HB2 H 3.964 0.01 2 31 6 6 SER HB3 H 3.892 0.01 2 32 6 6 SER N N 118.43 0.10 1 33 7 7 PRO HA H 4.381 0.01 1 34 7 7 PRO HB2 H 2.328 0.01 2 35 7 7 PRO HB3 H 1.910 0.01 2 36 7 7 PRO HG2 H 2.058 0.01 1 37 7 7 PRO HG3 H 2.058 0.01 1 38 7 7 PRO HD2 H 3.843 0.01 1 39 7 7 PRO HD3 H 3.843 0.01 1 40 8 8 GLU H H 8.629 0.01 1 41 8 8 GLU HA H 4.129 0.01 1 42 8 8 GLU HB2 H 1.914 0.01 1 43 8 8 GLU HB3 H 1.914 0.01 1 44 8 8 GLU HG2 H 2.194 0.01 2 45 8 8 GLU HG3 H 2.164 0.01 2 46 8 8 GLU N N 119.48 0.10 1 47 9 9 HIS H H 8.195 0.01 1 48 9 9 HIS HA H 4.556 0.01 1 49 9 9 HIS HB2 H 3.138 0.01 2 50 9 9 HIS HB3 H 3.209 0.01 2 51 9 9 HIS HD2 H 7.102 0.01 1 52 9 9 HIS HE1 H 8.050 0.01 1 53 10 10 GLN H H 8.263 0.01 1 54 10 10 GLN HA H 4.205 0.01 1 55 10 10 GLN HB2 H 2.095 0.01 2 56 10 10 GLN HB3 H 1.950 0.01 2 57 10 10 GLN HG2 H 2.305 0.01 1 58 10 10 GLN HG3 H 2.305 0.01 1 59 10 10 GLN HE21 H 6.952 0.01 1 60 10 10 GLN HE22 H 7.564 0.01 1 61 10 10 GLN N N 120.57 0.10 1 62 11 11 ARG H H 8.331 0.01 1 63 11 11 ARG HA H 4.274 0.01 1 64 11 11 ARG HB2 H 1.846 0.01 2 65 11 11 ARG HB3 H 1.814 0.01 2 66 11 11 ARG HG2 H 1.664 0.01 2 67 11 11 ARG HG3 H 1.602 0.01 2 68 11 11 ARG HD2 H 3.202 0.01 1 69 11 11 ARG HD3 H 3.202 0.01 1 70 11 11 ARG HE H 7.293 0.01 1 71 12 12 VAL H H 8.215 0.01 1 72 12 12 VAL HA H 3.997 0.01 1 73 12 12 VAL HB H 2.070 0.01 1 74 12 12 VAL HG1 H 0.976 0.01 2 75 12 12 VAL HG2 H 0.925 0.01 2 76 12 12 VAL N N 121.45 0.10 1 77 13 13 GLN H H 8.488 0.01 1 78 13 13 GLN HA H 4.259 0.01 1 79 13 13 GLN HB2 H 2.077 0.01 2 80 13 13 GLN HB3 H 1.991 0.01 2 81 13 13 GLN HG2 H 2.362 0.01 1 82 13 13 GLN HG3 H 2.362 0.01 1 83 13 13 GLN HE21 H 6.968 0.01 5 84 13 13 GLN HE22 H 7.599 0.01 1 85 14 14 GLN H H 8.525 0.01 1 86 14 14 GLN HA H 4.265 0.01 1 87 14 14 GLN HB2 H 2.081 0.01 2 88 14 14 GLN HB3 H 1.997 0.01 2 89 14 14 GLN HG2 H 2.381 0.01 1 90 14 14 GLN HG3 H 2.381 0.01 1 91 14 14 GLN HE21 H 6.968 0.01 5 92 14 14 GLN HE22 H 7.652 0.01 1 93 14 14 GLN N N 122.08 0.10 1 94 15 15 ARG H H 8.470 0.01 1 95 15 15 ARG HA H 4.269 0.01 1 96 15 15 ARG HB2 H 1.853 0.01 2 97 15 15 ARG HB3 H 1.797 0.01 2 98 15 15 ARG HG2 H 1.667 0.01 2 99 15 15 ARG HG3 H 1.605 0.01 2 100 15 15 ARG HD2 H 3.201 0.01 1 101 15 15 ARG HD3 H 3.201 0.01 1 102 15 15 ARG HE H 7.293 0.01 1 103 16 16 LYS H H 8.536 0.01 1 104 16 16 LYS HA H 4.293 0.01 1 105 16 16 LYS HB2 H 1.792 0.01 2 106 16 16 LYS HB3 H 1.847 0.01 2 107 16 16 LYS HG2 H 1.466 0.01 2 108 16 16 LYS HG3 H 1.425 0.01 2 109 16 16 LYS HD2 H 1.680 0.01 1 110 16 16 LYS HD3 H 1.680 0.01 1 111 16 16 LYS HE2 H 2.992 0.01 1 112 16 16 LYS HE3 H 2.992 0.01 1 113 17 17 GLU H H 8.673 0.01 1 114 17 17 GLU HA H 4.307 0.01 1 115 17 17 GLU HB2 H 1.973 0.01 1 116 17 17 GLU HB3 H 1.973 0.01 1 117 17 17 GLU HG2 H 2.307 0.01 2 118 17 17 GLU HG3 H 2.096 0.01 2 119 17 17 GLU N N 122.57 0.10 1 120 18 18 SER H H 8.428 0.01 1 121 18 18 SER HA H 4.388 0.01 1 122 18 18 SER HB2 H 3.909 0.01 2 123 18 18 SER HB3 H 3.865 0.01 2 124 18 18 SER N N 117.55 0.10 1 stop_ save_ save_assigned_chemical_shifts_1_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '3D 1H-1H-15N NOESY-HMQC' '2D 1H-1H TOCSY' '2D 1H-15N SOFAST-HMQC' stop_ loop_ _Sample_label $sample_1 $sample_2 $sample_3 $sample_4 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name entity_2 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 101 1 FKZ H H 8.060 0.01 1 2 101 1 FKZ H11 H 3.104 0.01 1 3 101 1 FKZ H12 H 3.104 0.01 1 4 101 1 FKZ H21 H 1.438 0.01 1 5 101 1 FKZ H22 H 1.438 0.01 1 6 101 1 FKZ H31 H 1.253 0.01 1 7 101 1 FKZ H32 H 1.253 0.01 1 8 101 1 FKZ H41 H 1.253 0.01 1 9 101 1 FKZ H42 H 1.253 0.01 1 10 101 1 FKZ H51 H 1.253 0.01 1 11 101 1 FKZ H52 H 1.253 0.01 1 12 101 1 FKZ H61 H 1.253 0.01 1 13 101 1 FKZ H62 H 1.253 0.01 1 14 101 1 FKZ H71 H 1.253 0.01 1 15 101 1 FKZ H72 H 1.253 0.01 1 16 101 1 FKZ H81 H 0.850 0.01 1 17 101 1 FKZ H82 H 0.850 0.01 1 18 101 1 FKZ H83 H 0.850 0.01 1 stop_ save_