data_356 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Conformational behavior of fragments of adrenocorticotropin and their antisense peptides determined by NMR spectroscopy and CD spectropolarimetry ; _BMRB_accession_number 356 _BMRB_flat_file_name bmr356.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Najem Elias J. . 2 Corigliano-Murphy Angela . . 3 Ferretti James A. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 148 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-11 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Najem, Elias J., Corigliano-Murphy, Angela, Ferretti, James A., "Conformational behavior of fragments of adrenocorticotropin and their antisense peptides determined by NMR spectroscopy and CD spectropolarimetry," FEBS Lett. 250 (2), 405-410 (1989). ; _Citation_title ; Conformational behavior of fragments of adrenocorticotropin and their antisense peptides determined by NMR spectroscopy and CD spectropolarimetry ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Najem Elias J. . 2 Corigliano-Murphy Angela . . 3 Ferretti James A. . stop_ _Journal_abbreviation 'FEBS Lett.' _Journal_volume 250 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 405 _Page_last 410 _Year 1989 _Details . save_ ################################## # Molecular system description # ################################## save_system_adrenocorticotropin _Saveframe_category molecular_system _Mol_system_name adrenocorticotropin _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label adrenocorticotropin $adrenocorticotropin stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_adrenocorticotropin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common adrenocorticotropin _Name_variant 'ACTH residues 1-24' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 24 _Mol_residue_sequence ; SYSMEHFRWGKPVGKKRRPV KVYP ; loop_ _Residue_seq_code _Residue_label 1 SER 2 TYR 3 SER 4 MET 5 GLU 6 HIS 7 PHE 8 ARG 9 TRP 10 GLY 11 LYS 12 PRO 13 VAL 14 GLY 15 LYS 16 LYS 17 ARG 18 ARG 19 PRO 20 VAL 21 LYS 22 VAL 23 TYR 24 PRO stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-29 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAB30771 "unnamed protein product [Mus musculus]" 100.00 235 100.00 100.00 1.75e-07 DBJ BAB30818 "unnamed protein product [Mus musculus]" 100.00 235 100.00 100.00 1.75e-07 DBJ BAC27095 "unnamed protein product [Mus musculus]" 100.00 235 100.00 100.00 1.75e-07 DBJ BAC36795 "unnamed protein product [Mus musculus]" 100.00 235 100.00 100.00 1.75e-07 DBJ BAE21739 "unnamed protein product [Mus musculus]" 100.00 235 100.00 100.00 1.75e-07 EMBL CAA23440 "unnamed protein product [Bos taurus]" 100.00 265 100.00 100.00 1.28e-07 EMBL CAA23441 "unnamed protein product, partial [Bos taurus]" 100.00 191 100.00 100.00 7.16e-08 EMBL CAA24754 "proopiomelanocortin [Homo sapiens]" 100.00 267 100.00 100.00 1.86e-07 EMBL CAA24770 "unnamed protein product [Mus musculus]" 100.00 191 100.00 100.00 1.55e-07 EMBL CAA24968 "POMC [Sus scrofa]" 100.00 267 100.00 100.00 2.06e-07 GB AAA30414 "ATCH-beta-lipotropin precursor [Bos taurus]" 100.00 187 100.00 100.00 6.47e-08 GB AAA30718 "preproadrenocorticotropic hormone (ACTH), partial [Bos taurus]" 100.00 67 100.00 100.00 2.09e-08 GB AAA30967 "proopiomelanocortin, partial [Neovison vison]" 100.00 142 100.00 100.00 7.65e-08 GB AAA37169 "adrenocorticotropic hormone precursor, partial [Mus musculus]" 100.00 39 100.00 100.00 5.27e-08 GB AAA41903 "preproopiomelanocortin [Rattus norvegicus]" 100.00 235 100.00 100.00 1.75e-07 PRF 0411225A "corticotropin inhibiting peptide" 75.00 32 100.00 100.00 3.70e-03 PRF 0505581A corticotropin 100.00 265 100.00 100.00 1.28e-07 PRF 0511280A "melanotropin alpha" 54.17 13 100.00 100.00 5.09e+00 PRF 0512240A melanotropin,N,O-(Ac)2 54.17 13 100.00 100.00 5.09e+00 PRF 0611192A "corticotropin 38" 100.00 38 100.00 100.00 5.43e-08 REF NP_000930 "pro-opiomelanocortin preproprotein [Homo sapiens]" 100.00 267 100.00 100.00 1.86e-07 REF NP_001009266 "pro-opiomelanocortin precursor [Ovis aries]" 100.00 263 100.00 100.00 1.38e-07 REF NP_001030333 "pro-opiomelanocortin preproprotein [Homo sapiens]" 100.00 267 100.00 100.00 1.86e-07 REF NP_001185504 "pro-opiomelanocortin A1 precursor [Salmo salar]" 95.83 232 100.00 100.00 1.31e-06 REF NP_001265510 "pro-opiomelanocortin preproprotein [Mus musculus]" 100.00 235 100.00 100.00 1.75e-07 SP P01189 "RecName: Full=Pro-opiomelanocortin; Short=POMC; AltName: Full=Corticotropin-lipotropin; Contains: RecName: Full=NPP; Contains: " 100.00 267 100.00 100.00 1.86e-07 SP P01190 "RecName: Full=Pro-opiomelanocortin; Short=POMC; AltName: Full=Corticotropin-lipotropin; Contains: RecName: Full=NPP; Contains: " 100.00 265 100.00 100.00 1.28e-07 SP P01191 "RecName: Full=Pro-opiomelanocortin; Short=POMC; AltName: Full=Corticotropin-lipotropin; Contains: RecName: Full=NPP; Contains: " 100.00 263 100.00 100.00 1.41e-07 SP P01192 "RecName: Full=Pro-opiomelanocortin; Short=POMC; AltName: Full=Corticotropin-lipotropin; Contains: RecName: Full=NPP; Contains: " 100.00 267 100.00 100.00 2.06e-07 SP P01193 "RecName: Full=Pro-opiomelanocortin; Short=POMC; AltName: Full=Corticotropin-lipotropin; Contains: RecName: Full=NPP; Contains: " 100.00 235 100.00 100.00 1.75e-07 TPG DAA24362 "TPA: pro-opiomelanocortin [Bos taurus]" 100.00 265 100.00 100.00 1.28e-07 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $adrenocorticotropin cow 9913 Eukaryota Metazoa Bos taurus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $adrenocorticotropin 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.6 . na temperature 291 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details 'The chemical shift reference is not available at this time.' save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name adrenocorticotropin _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 SER HA H 4.04 . 1 2 . 1 SER HB2 H 3.93 . 1 3 . 1 SER HB3 H 3.93 . 1 4 . 2 TYR H H 8.83 . 1 5 . 2 TYR HA H 4.59 . 1 6 . 2 TYR HB2 H 3.09 . 2 7 . 2 TYR HB3 H 2.92 . 2 8 . 3 SER H H 8.43 . 1 9 . 3 SER HA H 4.3 . 1 10 . 3 SER HB2 H 3.82 . 2 11 . 3 SER HB3 H 3.95 . 2 12 . 4 MET H H 8.27 . 1 13 . 4 MET HA H 4.46 . 1 14 . 4 MET HB2 H 2.63 . 2 15 . 4 MET HB3 H 2.14 . 2 16 . 5 GLU H H 8.07 . 1 17 . 5 GLU HA H 4.19 . 1 18 . 5 GLU HB2 H 1.96 . 2 19 . 5 GLU HB3 H 2.04 . 2 20 . 5 GLU HG2 H 2.43 . 1 21 . 5 GLU HG3 H 2.43 . 1 22 . 6 HIS H H 8.12 . 1 23 . 6 HIS HA H 4.48 . 1 24 . 6 HIS HB2 H 3.07 . 2 25 . 6 HIS HB3 H 3.12 . 2 26 . 7 PHE H H 8.06 . 1 27 . 7 PHE HA H 4.61 . 1 28 . 7 PHE HB2 H 2.75 . 2 29 . 7 PHE HB3 H 2.85 . 2 30 . 8 ARG H H 8.27 . 1 31 . 8 ARG HA H 4.6 . 1 32 . 8 ARG HB2 H 1.8 . 1 33 . 8 ARG HB3 H 1.8 . 1 34 . 8 ARG HG2 H 1.7 . 1 35 . 8 ARG HG3 H 1.7 . 1 36 . 8 ARG HD2 H 3.2 . 1 37 . 8 ARG HD3 H 3.2 . 1 38 . 9 TRP H H 8.09 . 1 39 . 9 TRP HA H 4.55 . 1 40 . 9 TRP HB2 H 3.2 . 2 41 . 9 TRP HB3 H 3.31 . 2 42 . 10 GLY H H 8.28 . 1 43 . 10 GLY HA2 H 4.04 . 2 44 . 10 GLY HA3 H 3.85 . 2 45 . 11 LYS H H 8.06 . 1 46 . 11 LYS HA H 4.62 . 1 47 . 11 LYS HB2 H 1.69 . 2 48 . 11 LYS HB3 H 1.76 . 2 49 . 11 LYS HG2 H 1.4 . 2 50 . 11 LYS HG3 H 1.47 . 2 51 . 11 LYS HD2 H 1.8 . 1 52 . 11 LYS HD3 H 1.8 . 1 53 . 11 LYS HE2 H 3.2 . 1 54 . 11 LYS HE3 H 3.2 . 1 55 . 11 LYS HZ H 7.46 . 1 56 . 12 PRO HA H 4.5 . 1 57 . 12 PRO HB2 H 2.15 . 1 58 . 12 PRO HB3 H 2.15 . 1 59 . 12 PRO HG2 H 1.95 . 1 60 . 12 PRO HG3 H 1.95 . 1 61 . 12 PRO HD2 H 3.66 . 2 62 . 12 PRO HD3 H 3.78 . 2 63 . 13 VAL H H 8.18 . 1 64 . 13 VAL HA H 4.1 . 1 65 . 13 VAL HB H 2.05 . 1 66 . 13 VAL HG1 H .95 . 1 67 . 13 VAL HG2 H .95 . 1 68 . 14 GLY H H 8.47 . 1 69 . 14 GLY HA2 H 3.95 . 2 70 . 14 GLY HA3 H 3.82 . 2 71 . 15 LYS H H 8.19 . 1 72 . 15 LYS HA H 4.35 . 1 73 . 15 LYS HB2 H 1.64 . 2 74 . 15 LYS HB3 H 1.7 . 2 75 . 15 LYS HG2 H 1.35 . 2 76 . 15 LYS HG3 H 1.45 . 2 77 . 15 LYS HD2 H 1.7 . 1 78 . 15 LYS HD3 H 1.7 . 1 79 . 15 LYS HE2 H 2.9 . 1 80 . 15 LYS HE3 H 2.9 . 1 81 . 15 LYS HZ H 7.82 . 1 82 . 16 LYS H H 8.22 . 1 83 . 16 LYS HA H 4.22 . 1 84 . 16 LYS HB2 H 1.72 . 1 85 . 16 LYS HB3 H 1.72 . 1 86 . 16 LYS HG2 H 1.49 . 1 87 . 16 LYS HG3 H 1.49 . 1 88 . 16 LYS HD2 H 1.72 . 1 89 . 16 LYS HD3 H 1.72 . 1 90 . 16 LYS HE2 H 3.12 . 1 91 . 16 LYS HE3 H 3.12 . 1 92 . 16 LYS HZ H 7.4 . 1 93 . 17 ARG H H 8.06 . 1 94 . 17 ARG HA H 4.62 . 1 95 . 17 ARG HB2 H 1.69 . 2 96 . 17 ARG HB3 H 1.76 . 2 97 . 17 ARG HG2 H 1.69 . 1 98 . 17 ARG HG3 H 1.69 . 1 99 . 17 ARG HD2 H 3.2 . 1 100 . 17 ARG HD3 H 3.2 . 1 101 . 17 ARG HE H 7.46 . 1 102 . 18 ARG H H 8.27 . 1 103 . 18 ARG HA H 4.6 . 1 104 . 18 ARG HB2 H 1.86 . 1 105 . 18 ARG HB3 H 1.86 . 1 106 . 18 ARG HG2 H 1.7 . 1 107 . 18 ARG HG3 H 1.7 . 1 108 . 18 ARG HD2 H 3.2 . 1 109 . 18 ARG HD3 H 3.2 . 1 110 . 18 ARG HE H 7.46 . 1 111 . 19 PRO HA H 4.5 . 1 112 . 19 PRO HB2 H 2.15 . 1 113 . 19 PRO HB3 H 2.15 . 1 114 . 19 PRO HG2 H 1.95 . 1 115 . 19 PRO HG3 H 1.95 . 1 116 . 19 PRO HD2 H 3.66 . 2 117 . 19 PRO HD3 H 3.78 . 2 118 . 20 VAL H H 8.13 . 1 119 . 20 VAL HA H 4.18 . 1 120 . 20 VAL HB H 2.07 . 1 121 . 20 VAL HG1 H .93 . 1 122 . 20 VAL HG2 H .93 . 1 123 . 21 LYS H H 8.21 . 1 124 . 21 LYS HA H 4.36 . 1 125 . 21 LYS HB2 H 1.68 . 1 126 . 21 LYS HB3 H 1.68 . 1 127 . 21 LYS HG2 H 1.42 . 1 128 . 21 LYS HG3 H 1.42 . 1 129 . 21 LYS HD2 H 1.68 . 1 130 . 21 LYS HD3 H 1.68 . 1 131 . 21 LYS HE2 H 2.9 . 1 132 . 21 LYS HE3 H 2.9 . 1 133 . 22 VAL H H 7.99 . 1 134 . 22 VAL HA H 4.14 . 1 135 . 22 VAL HB H 1.95 . 1 136 . 22 VAL HG1 H .84 . 1 137 . 22 VAL HG2 H .84 . 1 138 . 23 TYR H H 8.24 . 1 139 . 23 TYR HA H 4.75 . 1 140 . 23 TYR HB2 H 2.79 . 2 141 . 23 TYR HB3 H 2.98 . 2 142 . 24 PRO HA H 4.35 . 1 143 . 24 PRO HB2 H 2.22 . 1 144 . 24 PRO HB3 H 2.22 . 1 145 . 24 PRO HG2 H 1.96 . 1 146 . 24 PRO HG3 H 1.96 . 1 147 . 24 PRO HD2 H 3.4 . 2 148 . 24 PRO HD3 H 3.72 . 2 stop_ save_