data_371 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Secondary Structure of a Leucine Zipper Determined by Nuclear Magnetic Resonance Spectroscopy ; _BMRB_accession_number 371 _BMRB_flat_file_name bmr371.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-04-13 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Oas Terrence G. . 2 McIntosh Lawrence P. . 3 O'Shea Erin . . 4 Dahlquist Frederick W. . 5 Kim Peter S. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 123 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-11 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-04-13 revision BMRB 'Link to the Protein Data Bank added' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Oas, Terrence G., McIntosh, Lawrence P., O'Shea, Erin, Dahlquist, Frederick W., Kim, Peter S., "Secondary Structure of a Leucine Zipper Determined by Nuclear Magnetic Resonance Spectroscopy," Biochemistry 29, 2891-2894 (1990). ; _Citation_title ; Secondary Structure of a Leucine Zipper Determined by Nuclear Magnetic Resonance Spectroscopy ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Oas Terrence G. . 2 McIntosh Lawrence P. . 3 O'Shea Erin . . 4 Dahlquist Frederick W. . 5 Kim Peter S. . stop_ _Journal_abbreviation Biochemistry _Journal_volume 29 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2891 _Page_last 2894 _Year 1990 _Details . save_ ################################## # Molecular system description # ################################## save_system_transcriptional_regulator_GCN4 _Saveframe_category molecular_system _Mol_system_name 'transcriptional regulator GCN4' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'transcriptional regulator GCN4' $transcriptional_regulator_GCN4 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_transcriptional_regulator_GCN4 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'transcriptional regulator GCN4' _Name_variant 'residues 239-271' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 33 _Mol_residue_sequence ; XMKQLEDKVEELLSKNYHLE NEVARLKKLVGER ; loop_ _Residue_seq_code _Residue_label 1 X 2 MET 3 LYS 4 GLN 5 LEU 6 GLU 7 ASP 8 LYS 9 VAL 10 GLU 11 GLU 12 LEU 13 LEU 14 SER 15 LYS 16 ASN 17 TYR 18 HIS 19 LEU 20 GLU 21 ASN 22 GLU 23 VAL 24 ALA 25 ARG 26 LEU 27 LYS 28 LYS 29 LEU 30 VAL 31 GLY 32 GLU 33 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-15 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1396 "transcriptional regulator GCN4" 96.97 61 100.00 100.00 5.33e-12 BMRB 1397 "transcriptional regulator GCN4" 96.97 61 100.00 100.00 5.33e-12 BMRB 1398 "transcriptional regulator GCN4" 96.97 61 100.00 100.00 5.33e-12 BMRB 1399 "transcriptional regulator GCN4" 96.97 61 100.00 100.00 5.33e-12 BMRB 1451 "transcriptional regulator GCN4" 96.97 61 100.00 100.00 5.33e-12 BMRB 1452 "transcriptional regulator GCN4" 96.97 61 100.00 100.00 5.33e-12 BMRB 1453 "transcriptional regulator GCN4" 96.97 61 100.00 100.00 5.33e-12 BMRB 1454 "transcriptional regulator GCN4" 96.97 61 100.00 100.00 5.33e-12 BMRB 15316 GCN4p-wt 93.94 33 100.00 100.00 1.02e-10 BMRB 17511 LZ-GCN4 96.97 36 100.00 100.00 1.81e-11 BMRB 2062 "transcriptional regulator GCN4-p2N" 96.97 37 100.00 100.00 1.78e-11 PDB 1DGC "The X-Ray Structure Of The Gcn4-Bzip Bound To AtfCREB SITE Dna Shows The Complex Depends On Dna Flexibility" 96.97 62 100.00 100.00 5.27e-12 PDB 1LD4 "Placement Of The Structural Proteins In Sindbis Virus" 96.97 57 100.00 100.00 5.67e-12 PDB 1LLM "Crystal Structure Of A Zif23-Gcn4 Chimera Bound To Dna" 87.88 88 100.00 100.00 2.12e-09 PDB 1NKN "Visualizing An Unstable Coiled Coil: The Crystal Structure Of An N-Terminal Segment Of The Scallop Myosin Rod" 96.97 89 100.00 100.00 2.08e-11 PDB 1YSA "The Gcn4 Basic Region Leucine Zipper Binds Dna As A Dimer Of Uninterrupted Alpha Helices: Crystal Structure Of The Protein-Dna " 96.97 58 100.00 100.00 6.86e-12 PDB 1ZTA "The Solution Structure Of A Leucine-Zipper Motif Peptide" 96.97 35 100.00 100.00 1.68e-11 PDB 2DGC "Gcn4 Basic Domain, Leucine Zipper Complexed With AtfCREB Site Dna" 96.97 63 100.00 100.00 5.49e-12 PDB 2EFR "Crystal Structure Of The C-Terminal Tropomyosin Fragment With N- And C-Terminal Extensions Of The Leucine Zipper At 1.8 Angstro" 84.85 155 100.00 100.00 3.32e-08 PDB 2EFS "Crystal Structure Of The C-Terminal Tropomyosin Fragment With N- And C-Terminal Extensions Of The Leucine Zipper At 2.0 Angstro" 84.85 155 100.00 100.00 3.32e-08 PDB 2OVN "Nmr Structure Of The Gcn4 Trigger Peptide" 51.52 17 100.00 100.00 1.64e-01 PDB 2ZTA "X-Ray Structure Of The Gcn4 Leucine Zipper, A Two-Stranded, Parallel Coiled Coil" 96.97 34 100.00 100.00 1.77e-11 PDB 3BAS "Crystal Structure Of The N-Terminal Region Of The Scallop Myosin Rod, Monoclinic (C2) Form" 96.97 89 100.00 100.00 2.08e-11 PDB 3BAT "Crystal Structure Of The N-Terminal Region Of The Scallop Myosin Rod, Monoclinic (P21) Form" 96.97 89 100.00 100.00 2.08e-11 PDB 3I5C "Crystal Structure Of A Fusion Protein Containing The Leucine Zipper Of Gcn4 And The Ggdef Domain Of Wspr From Pseudomonas Aerug" 87.88 206 100.00 100.00 4.25e-09 PDB 3P8M "Human Dynein Light Chain (Dynll2) In Complex With An In Vitro Evolved Peptide Dimerized By Leucine Zipper" 96.97 46 100.00 100.00 6.08e-12 PDB 4DMD "Gcn4 Leucine Zipper Domain In A Dimeric Oligomerization State" 96.97 34 100.00 100.00 1.77e-11 PDB 4DME "Gcn4 Leucine Zipper Domain In A Trimeric Oligomerization State" 96.97 35 100.00 100.00 1.79e-11 DBJ GAA22815 "K7_Gcn4p [Saccharomyces cerevisiae Kyokai no. 7]" 96.97 281 100.00 100.00 1.02e-11 EMBL CAE52206 "Gcn4p [Saccharomyces cerevisiae]" 96.97 281 100.00 100.00 1.61e-09 EMBL CAE52207 "Gcn4p [Saccharomyces cerevisiae]" 96.97 281 100.00 100.00 1.61e-09 EMBL CAE52208 "Gcn4p [Saccharomyces cerevisiae]" 96.97 281 100.00 100.00 1.31e-09 EMBL CAE52209 "Gcn4p [Saccharomyces cerevisiae]" 96.97 281 100.00 100.00 1.61e-09 EMBL CAE52210 "Gcn4p [Saccharomyces cerevisiae]" 96.97 281 100.00 100.00 1.02e-11 GB AAA34640 "GCN4 protein [Saccharomyces cerevisiae]" 96.97 281 100.00 100.00 1.70e-09 GB AAB64486 "Transcriptional activator of amino acid biosynthetic genes [Saccharomyces cerevisiae]" 96.97 281 100.00 100.00 1.70e-09 GB AAC33186 "hinge-region and leucine-zipper with N-terminal PelB-leader and C-terminal hexahistidine tag [synthetic construct]" 96.97 84 100.00 100.00 4.12e-12 GB AAD37724 "PelB-IgG kappa light chain fusion protein [Cloning vector pCLZip]" 96.97 192 100.00 100.00 1.13e-11 GB AAK07887 "cI-GCN4 repressor fusion protein [Cloning vector pJH370]" 96.97 166 100.00 100.00 2.68e-11 REF NP_010907 "amino acid starvation-responsive transcription factor GCN4 [Saccharomyces cerevisiae S288c]" 96.97 281 100.00 100.00 1.70e-09 SP P03069 "RecName: Full=General control protein GCN4; AltName: Full=Amino acid biosynthesis regulatory protein" 96.97 281 100.00 100.00 1.70e-09 TPG DAA07643 "TPA: amino acid starvation-responsive transcription factor GCN4 [Saccharomyces cerevisiae S288c]" 96.97 281 100.00 100.00 1.70e-09 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $transcriptional_regulator_GCN4 yeast 4932 Eukaryota Fungi Saccharomyces cerevisiae KY803 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $transcriptional_regulator_GCN4 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5 . na temperature 293 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label TSP H . . ppm -.01 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'transcriptional regulator GCN4' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 2 MET H H 8.61 . 1 2 . 2 MET HA H 4.07 . 1 3 . 2 MET HB2 H 2.08 . 1 4 . 2 MET HB3 H 2.08 . 1 5 . 3 LYS H H 7.83 . 1 6 . 3 LYS HA H 4.14 . 1 7 . 3 LYS HB2 H 1.84 . 1 8 . 3 LYS HB3 H 1.84 . 1 9 . 4 GLN H H 7.78 . 1 10 . 4 GLN HA H 4.13 . 1 11 . 4 GLN HB2 H 2.22 . 1 12 . 4 GLN HB3 H 2.22 . 1 13 . 5 LEU H H 8.23 . 1 14 . 5 LEU HA H 4.08 . 1 15 . 5 LEU HB2 H 2.13 . 2 16 . 5 LEU HB3 H 1.34 . 2 17 . 6 GLU H H 8.56 . 1 18 . 6 GLU HA H 3.86 . 1 19 . 6 GLU HB2 H 2.28 . 1 20 . 6 GLU HB3 H 2.28 . 1 21 . 7 ASP H H 8.73 . 1 22 . 7 ASP HA H 4.39 . 1 23 . 7 ASP HB2 H 2.85 . 2 24 . 7 ASP HB3 H 2.68 . 2 25 . 8 LYS H H 8.03 . 1 26 . 8 LYS HA H 4.23 . 1 27 . 8 LYS HB2 H 2.13 . 2 28 . 8 LYS HB3 H 2.03 . 2 29 . 9 VAL H H 8.51 . 1 30 . 9 VAL HA H 3.42 . 1 31 . 9 VAL HB H 2.27 . 1 32 . 10 GLU H H 7.75 . 1 33 . 10 GLU HA H 4 . 1 34 . 10 GLU HB2 H 2.18 . 1 35 . 10 GLU HB3 H 2.18 . 1 36 . 11 GLU H H 8.24 . 1 37 . 11 GLU HA H 4.11 . 1 38 . 11 GLU HB2 H 2.3 . 2 39 . 11 GLU HB3 H 2.28 . 2 40 . 12 LEU H H 8.74 . 1 41 . 12 LEU HA H 4.01 . 1 42 . 12 LEU HB2 H 2.09 . 2 43 . 12 LEU HB3 H 1.27 . 2 44 . 13 LEU H H 9.05 . 1 45 . 13 LEU HA H 4.06 . 1 46 . 13 LEU HB2 H 1.96 . 2 47 . 13 LEU HB3 H 1.46 . 2 48 . 14 SER H H 7.78 . 1 49 . 14 SER HA H 4.36 . 1 50 . 14 SER HB2 H 4.09 . 1 51 . 14 SER HB3 H 4.09 . 1 52 . 15 LYS H H 8.33 . 1 53 . 15 LYS HA H 4.21 . 1 54 . 15 LYS HB2 H 1.94 . 1 55 . 15 LYS HB3 H 1.94 . 1 56 . 16 ASN H H 8.87 . 1 57 . 16 ASN HA H 4.39 . 1 58 . 16 ASN HB2 H 3.28 . 2 59 . 16 ASN HB3 H 2.75 . 2 60 . 17 TYR H H 8.26 . 1 61 . 17 TYR HA H 4.39 . 1 62 . 17 TYR HB2 H 3.28 . 2 63 . 17 TYR HB3 H 3.15 . 2 64 . 18 HIS H H 7.93 . 1 65 . 18 HIS HA H 4.38 . 1 66 . 18 HIS HB2 H 3.44 . 1 67 . 18 HIS HB3 H 3.44 . 1 68 . 19 LEU H H 8.66 . 1 69 . 19 LEU HA H 3.98 . 1 70 . 19 LEU HB2 H 2.16 . 2 71 . 19 LEU HB3 H 1.33 . 2 72 . 20 GLU H H 8.88 . 1 73 . 20 GLU HA H 3.94 . 1 74 . 20 GLU HB2 H 2.19 . 2 75 . 20 GLU HB3 H 2.01 . 2 76 . 21 ASN H H 7.77 . 1 77 . 21 ASN HA H 4.44 . 1 78 . 21 ASN HB2 H 2.84 . 2 79 . 21 ASN HB3 H 2.76 . 2 80 . 22 GLU H H 8.04 . 1 81 . 22 GLU HA H 4.39 . 1 82 . 22 GLU HB2 H 2.16 . 2 83 . 22 GLU HB3 H 2.02 . 2 84 . 23 VAL H H 8.68 . 1 85 . 23 VAL HA H 3.39 . 1 86 . 23 VAL HB H 2.12 . 1 87 . 24 ALA H H 7.78 . 1 88 . 24 ALA HA H 4.01 . 1 89 . 24 ALA HB H 1.49 . 1 90 . 25 ARG H H 7.94 . 1 91 . 25 ARG HA H 3.98 . 1 92 . 25 ARG HB2 H 2.13 . 1 93 . 25 ARG HB3 H 2.13 . 1 94 . 26 LEU H H 8.47 . 1 95 . 26 LEU HA H 3.98 . 1 96 . 26 LEU HB2 H 1.97 . 2 97 . 26 LEU HB3 H 1.32 . 2 98 . 27 LYS H H 9.01 . 1 99 . 27 LYS HA H 3.82 . 1 100 . 27 LYS HB2 H 1.83 . 1 101 . 27 LYS HB3 H 1.83 . 1 102 . 28 LYS H H 7.38 . 1 103 . 28 LYS HA H 4.13 . 1 104 . 28 LYS HB2 H 1.97 . 2 105 . 28 LYS HB3 H 1.9 . 2 106 . 29 LEU H H 7.49 . 1 107 . 29 LEU HA H 4.19 . 1 108 . 29 LEU HB2 H 2.05 . 2 109 . 29 LEU HB3 H 1.62 . 2 110 . 30 VAL H H 7.79 . 1 111 . 30 VAL HA H 4.08 . 1 112 . 30 VAL HB H 2.24 . 1 113 . 31 GLY H H 7.88 . 1 114 . 31 GLY HA2 H 3.9 . 2 115 . 31 GLY HA3 H 4.08 . 2 116 . 32 GLU H H 7.96 . 1 117 . 32 GLU HA H 4.38 . 1 118 . 32 GLU HB2 H 1.77 . 1 119 . 32 GLU HB3 H 1.77 . 1 120 . 33 ARG H H 7.99 . 1 121 . 33 ARG HA H 4.13 . 1 122 . 33 ARG HB2 H 1.83 . 2 123 . 33 ARG HB3 H 1.7 . 2 stop_ save_