data_373 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; A 1H NMR study of the solution conformation of the neuropeptide Galanin ; _BMRB_accession_number 373 _BMRB_flat_file_name bmr373.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Wennerberg Anders B.A. . 2 Cooke Robert M. . 3 Carlquist Mats . . 4 Rigler Rudolf . . 5 Campbell Iain D. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 79 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-11 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Wennerberg, Anders B.A., Cooke, Robert M., Carlquist, Mats, Rigler, Rudolf, Campbell, Iain D., "A 1H NMR study of the solution conformation of the neuropeptide Galanin," Biochem. Biophys. Res. Commun. 166 (3), 1102-1109 (1990). ; _Citation_title 'A 1H NMR study of the solution conformation of the neuropeptide Galanin' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Wennerberg Anders B.A. . 2 Cooke Robert M. . 3 Carlquist Mats . . 4 Rigler Rudolf . . 5 Campbell Iain D. . stop_ _Journal_abbreviation 'Biochem. Biophys. Res. Commun.' _Journal_volume 166 _Journal_issue 3 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1102 _Page_last 1109 _Year 1990 _Details . save_ ################################## # Molecular system description # ################################## save_system_galanin _Saveframe_category molecular_system _Mol_system_name galanin _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label galanin $galanin stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_galanin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common galanin _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 29 _Mol_residue_sequence ; GWTLNSAGYLLGPHAIDNHR SFSDKHGLT ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 TRP 3 THR 4 LEU 5 ASN 6 SER 7 ALA 8 GLY 9 TYR 10 LEU 11 LEU 12 GLY 13 PRO 14 HIS 15 ALA 16 ILE 17 ASP 18 ASN 19 HIS 20 ARG 21 SER 22 PHE 23 SER 24 ASP 25 LYS 26 HIS 27 GLY 28 LEU 29 THR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-10-26 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 372 galanin 100.00 29 100.00 100.00 1.45e-11 DBJ BAE21703 "unnamed protein product [Mus musculus]" 100.00 124 100.00 100.00 6.20e-12 EMBL CAA80610 "galanin [Mus musculus]" 100.00 99 100.00 100.00 3.52e-12 GB AAA41186 "galanin precursor [Rattus norvegicus]" 100.00 124 100.00 100.00 5.77e-12 GB AAA41187 "galanin [Rattus norvegicus]" 100.00 124 100.00 100.00 5.77e-12 GB AAB41545 "galanin [Mus musculus]" 100.00 124 100.00 100.00 6.20e-12 GB AAH44055 "Galanin [Mus musculus]" 100.00 124 100.00 100.00 6.20e-12 GB EDL32939 "galanin, isoform CRA_a [Mus musculus]" 100.00 141 100.00 100.00 1.23e-12 REF NP_034383 "galanin peptides precursor [Mus musculus]" 100.00 124 100.00 100.00 6.20e-12 REF NP_150240 "galanin peptides preproprotein [Rattus norvegicus]" 100.00 124 100.00 100.00 5.77e-12 REF XP_003510421 "PREDICTED: galanin peptides [Cricetulus griseus]" 100.00 124 100.00 100.00 7.48e-12 REF XP_005064159 "PREDICTED: galanin peptides [Mesocricetus auratus]" 100.00 124 100.00 100.00 7.32e-12 REF XP_006980557 "PREDICTED: galanin peptides [Peromyscus maniculatus bairdii]" 100.00 124 100.00 100.00 7.88e-12 SP P10683 "RecName: Full=Galanin peptides; Contains: RecName: Full=Galanin; Contains: RecName: Full=Galanin message-associated peptide; Sh" 100.00 124 100.00 100.00 5.77e-12 SP P47212 "RecName: Full=Galanin peptides; Contains: RecName: Full=Galanin; Contains: RecName: Full=Galanin message-associated peptide; Sh" 100.00 124 100.00 100.00 6.20e-12 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $galanin Rat 10116 Eukaryota Metazoa Rattus norvegicus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $galanin 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 2.6 . na temperature 300 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label p-dioxane H . . ppm 3.75 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name galanin _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 2 TRP HD1 H 7.23 . 1 2 . 2 TRP HE1 H 10.08 . 1 3 . 2 TRP HE3 H 7.59 . 1 4 . 2 TRP HZ2 H 7.47 . 1 5 . 2 TRP HZ3 H 7.13 . 1 6 . 2 TRP HH2 H 7.23 . 1 7 . 3 THR H H 8 . 1 8 . 3 THR HA H 4.25 . 1 9 . 3 THR HB H 4.09 . 1 10 . 3 THR HG2 H 1.1 . 1 11 . 4 LEU H H 8.13 . 1 12 . 4 LEU HA H 4.16 . 1 13 . 4 LEU HG H 1.54 . 1 14 . 4 LEU HD1 H .92 . 2 15 . 4 LEU HD2 H .86 . 2 16 . 5 ASN H H 7.86 . 1 17 . 5 ASN HA H 4.38 . 1 18 . 5 ASN HB2 H 3.02 . 2 19 . 5 ASN HB3 H 2.97 . 2 20 . 7 ALA H H 8.13 . 1 21 . 7 ALA HA H 4.29 . 1 22 . 7 ALA HB H 1.36 . 1 23 . 9 TYR HD1 H 7.07 . 1 24 . 9 TYR HD2 H 7.07 . 1 25 . 9 TYR HE1 H 6.8 . 1 26 . 9 TYR HE2 H 6.8 . 1 27 . 10 LEU H H 8.05 . 1 28 . 10 LEU HA H 4.25 . 1 29 . 10 LEU HG H 1.51 . 1 30 . 10 LEU HD1 H .9 . 2 31 . 10 LEU HD2 H .84 . 2 32 . 11 LEU H H 7.96 . 1 33 . 11 LEU HA H 4.37 . 1 34 . 11 LEU HG H 1.6 . 1 35 . 11 LEU HD1 H .91 . 2 36 . 11 LEU HD2 H .85 . 2 37 . 13 PRO HA H 4.35 . 1 38 . 13 PRO HB2 H 2.21 . 2 39 . 13 PRO HB3 H 1.78 . 2 40 . 13 PRO HG2 H 1.97 . 2 41 . 13 PRO HG3 H 1.87 . 2 42 . 13 PRO HD2 H 3.57 . 1 43 . 13 PRO HD3 H 3.57 . 1 44 . 15 ALA H H 8.26 . 1 45 . 15 ALA HA H 4.3 . 1 46 . 15 ALA HB H 1.37 . 1 47 . 16 ILE H H 8.14 . 1 48 . 16 ILE HA H 4.13 . 1 49 . 16 ILE HB H 1.83 . 1 50 . 16 ILE HG12 H 1.45 . 2 51 . 16 ILE HG13 H 1.18 . 2 52 . 16 ILE HG2 H .86 . 1 53 . 18 ASN H H 8.34 . 1 54 . 18 ASN HA H 4.65 . 1 55 . 18 ASN HB2 H 3.15 . 2 56 . 18 ASN HB3 H 3.02 . 2 57 . 20 ARG H H 8.21 . 1 58 . 20 ARG HA H 4.32 . 1 59 . 20 ARG HB2 H 1.76 . 2 60 . 20 ARG HB3 H 1.69 . 2 61 . 20 ARG HG2 H 1.57 . 2 62 . 20 ARG HG3 H 1.54 . 2 63 . 20 ARG HD2 H 3.16 . 1 64 . 20 ARG HD3 H 3.16 . 1 65 . 25 LYS H H 8.21 . 1 66 . 25 LYS HA H 4.24 . 1 67 . 25 LYS HB2 H 1.78 . 2 68 . 25 LYS HB3 H 1.68 . 2 69 . 25 LYS HG2 H 1.65 . 2 70 . 25 LYS HG3 H 1.36 . 2 71 . 25 LYS HD2 H 1.79 . 1 72 . 25 LYS HD3 H 1.79 . 1 73 . 25 LYS HE2 H 2.95 . 1 74 . 25 LYS HE3 H 2.95 . 1 75 . 28 LEU H H 8.26 . 1 76 . 28 LEU HA H 4.42 . 1 77 . 28 LEU HG H 1.65 . 1 78 . 28 LEU HD1 H .93 . 2 79 . 28 LEU HD2 H .88 . 2 stop_ save_