data_50409 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; SoRNHI 15N-1H Backbone Chemical Shifts ; _BMRB_accession_number 50409 _BMRB_flat_file_name bmr50409.str _Entry_type original _Submission_date 2020-07-24 _Accession_date 2020-07-24 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Martin James A. . 2 Robustelli Paul . . 3 Palmer Arthur G. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 151 "15N chemical shifts" 151 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-07-30 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 50407 'V98A EcRNHI* (Cys-free) 15N-1H Backbone Chemical Shifts' 50408 'V98A EcRNHI 15N-1H Backbone Chemical Shifts' stop_ _Original_release_date 2020-07-24 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Quantifying the relationship between Conformational Dynamics and Enzymatic Activity in Ribonuclease HI Homologues ; _Citation_status submitted _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Martin James A. . 2 Robustelli Paul . . 3 Palmer Arthur G. . stop_ _Journal_abbreviation Biochemistry _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly_1 _Saveframe_category molecular_system _Mol_system_name SoRNHI _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label SoRNHI $entity_1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 158 _Mol_residue_sequence ; MTELKLIHIFTDGSCLGNPG PGGYGIVMNYKGHTKEMSDG FSLTTNNRMELLAPIVALEA LKEPCKIILTSDSQYMRQGI MTWIHGWKKKGWMTSNRTPV KNVDLWKRLDKAAQLHQIDW RWVKGHAGHAENERCDQLAR AAAEANPTQIDTGYQAES ; loop_ _Residue_seq_code _Residue_label 1 MET 2 THR 3 GLU 4 LEU 5 LYS 6 LEU 7 ILE 8 HIS 9 ILE 10 PHE 11 THR 12 ASP 13 GLY 14 SER 15 CYS 16 LEU 17 GLY 18 ASN 19 PRO 20 GLY 21 PRO 22 GLY 23 GLY 24 TYR 25 GLY 26 ILE 27 VAL 28 MET 29 ASN 30 TYR 31 LYS 32 GLY 33 HIS 34 THR 35 LYS 36 GLU 37 MET 38 SER 39 ASP 40 GLY 41 PHE 42 SER 43 LEU 44 THR 45 THR 46 ASN 47 ASN 48 ARG 49 MET 50 GLU 51 LEU 52 LEU 53 ALA 54 PRO 55 ILE 56 VAL 57 ALA 58 LEU 59 GLU 60 ALA 61 LEU 62 LYS 63 GLU 64 PRO 65 CYS 66 LYS 67 ILE 68 ILE 69 LEU 70 THR 71 SER 72 ASP 73 SER 74 GLN 75 TYR 76 MET 77 ARG 78 GLN 79 GLY 80 ILE 81 MET 82 THR 83 TRP 84 ILE 85 HIS 86 GLY 87 TRP 88 LYS 89 LYS 90 LYS 91 GLY 92 TRP 93 MET 94 THR 95 SER 96 ASN 97 ARG 98 THR 99 PRO 100 VAL 101 LYS 102 ASN 103 VAL 104 ASP 105 LEU 106 TRP 107 LYS 108 ARG 109 LEU 110 ASP 111 LYS 112 ALA 113 ALA 114 GLN 115 LEU 116 HIS 117 GLN 118 ILE 119 ASP 120 TRP 121 ARG 122 TRP 123 VAL 124 LYS 125 GLY 126 HIS 127 ALA 128 GLY 129 HIS 130 ALA 131 GLU 132 ASN 133 GLU 134 ARG 135 CYS 136 ASP 137 GLN 138 LEU 139 ALA 140 ARG 141 ALA 142 ALA 143 ALA 144 GLU 145 ALA 146 ASN 147 PRO 148 THR 149 GLN 150 ILE 151 ASP 152 THR 153 GLY 154 TYR 155 GLN 156 ALA 157 GLU 158 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source_1 _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 'Shewanella oneidensis' 70863 Bacteria . Shewanella oneidensis stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . Escherichia coli . plasmid pET-25b(+) stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $entity_1 325 uM 250 400 '[U-100% 13C; U-100% 15N]' 'deuterated sodium acetate' 50 mM . . 'natural abundance' 'sodium chloride' 50 mM . . 'natural abundance' 'sodium azide' 0.02 '% (w/v)' . . 'natural abundance' DTT 1 mM . . 'natural abundance' DSS 3 mM . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name NMRPipe _Version . loop_ _Task processing stop_ _Details . save_ save_software_2 _Saveframe_category software _Name NMRFAM-SPARKY _Version . loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_CBCA(CO)NH_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HN(CA)CO_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)CO' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.050 . M pH 5.5 . pH pressure 1 . atm temperature 300 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $software_1 $software_2 stop_ loop_ _Experiment_label '3D CBCA(CO)NH' '3D HNCO' '3D HNCACB' '3D HNCA' '3D HN(CA)CO' '2D 1H-15N HSQC' '3D 1H-15N NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SoRNHI _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 LEU H H 8.652 . . 2 4 4 LEU N N 125.271 . . 3 5 5 LYS H H 8.918 . . 4 5 5 LYS N N 124.573 . . 5 6 6 LEU H H 8.077 . . 6 6 6 LEU N N 124.748 . . 7 7 7 ILE H H 8.998 . . 8 7 7 ILE N N 126.589 . . 9 8 8 HIS H H 8.613 . . 10 8 8 HIS N N 124.362 . . 11 9 9 ILE H H 8.707 . . 12 9 9 ILE N N 122.677 . . 13 10 10 PHE H H 9.46 . . 14 10 10 PHE N N 129.724 . . 15 11 11 THR H H 7.837 . . 16 11 11 THR N N 109.477 . . 17 12 12 ASP H H 8.77 . . 18 12 12 ASP N N 117.184 . . 19 13 13 GLY H H 8.724 . . 20 13 13 GLY N N 107.083 . . 21 14 14 SER H H 8.79 . . 22 14 14 SER N N 113.966 . . 23 15 15 CYS H H 8.356 . . 24 15 15 CYS N N 121.926 . . 25 16 16 LEU H H 9.011 . . 26 16 16 LEU N N 127.128 . . 27 17 17 GLY H H 7.739 . . 28 17 17 GLY N N 108.233 . . 29 18 18 ASN H H 8.152 . . 30 18 18 ASN N N 116.085 . . 31 20 20 GLY H H 8.3 . . 32 20 20 GLY N N 106.445 . . 33 22 22 GLY H H 8.887 . . 34 22 22 GLY N N 107.853 . . 35 23 23 GLY H H 8.91 . . 36 23 23 GLY N N 105.654 . . 37 24 24 TYR H H 8.131 . . 38 24 24 TYR N N 113.367 . . 39 25 25 GLY H H 9.028 . . 40 25 25 GLY N N 105.664 . . 41 26 26 ILE H H 9.079 . . 42 26 26 ILE N N 123.968 . . 43 27 27 VAL H H 8.597 . . 44 27 27 VAL N N 125.452 . . 45 28 28 MET H H 9.318 . . 46 28 28 MET N N 125.088 . . 47 29 29 ASN H H 9.573 . . 48 29 29 ASN N N 122.765 . . 49 30 30 TYR H H 8.588 . . 50 30 30 TYR N N 124.717 . . 51 31 31 LYS H H 9.294 . . 52 31 31 LYS N N 126.382 . . 53 32 32 GLY H H 8.455 . . 54 32 32 GLY N N 106.652 . . 55 33 33 HIS H H 8.196 . . 56 33 33 HIS N N 119.346 . . 57 34 34 THR H H 8.725 . . 58 34 34 THR N N 120.267 . . 59 35 35 LYS H H 9.126 . . 60 35 35 LYS N N 126.978 . . 61 36 36 GLU H H 8.639 . . 62 36 36 GLU N N 123.194 . . 63 37 37 MET H H 9.044 . . 64 37 37 MET N N 119.565 . . 65 38 38 SER H H 8.211 . . 66 38 38 SER N N 111.764 . . 67 39 39 ASP H H 6.685 . . 68 39 39 ASP N N 115.234 . . 69 40 40 GLY H H 7.938 . . 70 40 40 GLY N N 104.255 . . 71 41 41 PHE H H 9.146 . . 72 41 41 PHE N N 116.669 . . 73 42 42 SER H H 9.218 . . 74 42 42 SER N N 116.12 . . 75 43 43 LEU H H 6.916 . . 76 43 43 LEU N N 118.497 . . 77 44 44 THR H H 8.589 . . 78 44 44 THR N N 123.803 . . 79 45 45 THR H H 8.438 . . 80 45 45 THR N N 106.511 . . 81 46 46 ASN H H 8.969 . . 82 46 46 ASN N N 120.483 . . 83 47 47 ASN H H 8.639 . . 84 47 47 ASN N N 115.615 . . 85 48 48 ARG H H 7.422 . . 86 48 48 ARG N N 115.593 . . 87 49 49 MET H H 7.669 . . 88 49 49 MET N N 117.629 . . 89 50 50 GLU H H 8.353 . . 90 50 50 GLU N N 116.873 . . 91 51 51 LEU H H 7.016 . . 92 51 51 LEU N N 113.598 . . 93 52 52 LEU H H 8.637 . . 94 52 52 LEU N N 118.896 . . 95 53 53 ALA H H 7.158 . . 96 53 53 ALA N N 116.149 . . 97 55 55 ILE H H 7.045 . . 98 55 55 ILE N N 117.63 . . 99 56 56 VAL H H 8.459 . . 100 56 56 VAL N N 119.5 . . 101 57 57 ALA H H 7.71 . . 102 57 57 ALA N N 120.546 . . 103 58 58 LEU H H 8.176 . . 104 58 58 LEU N N 113.526 . . 105 59 59 GLU H H 9.031 . . 106 59 59 GLU N N 118.916 . . 107 60 60 ALA H H 7.335 . . 108 60 60 ALA N N 119.462 . . 109 61 61 LEU H H 7.151 . . 110 61 61 LEU N N 119.738 . . 111 62 62 LYS H H 8.489 . . 112 62 62 LYS N N 120.952 . . 113 63 63 GLU H H 7.669 . . 114 63 63 GLU N N 117.629 . . 115 65 65 CYS H H 10.958 . . 116 65 65 CYS N N 125.117 . . 117 66 66 LYS H H 8.828 . . 118 66 66 LYS N N 123.347 . . 119 67 67 ILE H H 8.742 . . 120 67 67 ILE N N 124.587 . . 121 68 68 ILE H H 7.99 . . 122 68 68 ILE N N 127.377 . . 123 69 69 LEU H H 8.473 . . 124 69 69 LEU N N 136.357 . . 125 70 70 THR H H 8.639 . . 126 70 70 THR N N 123.194 . . 127 71 71 SER H H 8.3 . . 128 71 71 SER N N 120.565 . . 129 72 72 ASP H H 8.752 . . 130 72 72 ASP N N 124.046 . . 131 73 73 SER H H 8.337 . . 132 73 73 SER N N 114.438 . . 133 74 74 GLN H H 9.005 . . 134 74 74 GLN N N 132.122 . . 135 75 75 TYR H H 8.678 . . 136 75 75 TYR N N 123.096 . . 137 76 76 MET H H 8.448 . . 138 76 76 MET N N 117.865 . . 139 77 77 ARG H H 7.513 . . 140 77 77 ARG N N 118.518 . . 141 78 78 GLN H H 7.559 . . 142 78 78 GLN N N 115.784 . . 143 79 79 GLY H H 7.556 . . 144 79 79 GLY N N 107.702 . . 145 80 80 ILE H H 8.305 . . 146 80 80 ILE N N 120.592 . . 147 81 81 MET H H 8.22 . . 148 81 81 MET N N 114.469 . . 149 82 82 THR H H 7.39 . . 150 82 82 THR N N 111.829 . . 151 83 83 TRP H H 7.723 . . 152 83 83 TRP HE1 H 10.071 . . 153 83 83 TRP N N 121.7 . . 154 83 83 TRP NE1 N 128.585 . . 155 84 84 ILE H H 8.521 . . 156 84 84 ILE N N 117.619 . . 157 85 85 HIS H H 7.856 . . 158 85 85 HIS N N 117.513 . . 159 86 86 GLY H H 7.33 . . 160 86 86 GLY N N 105.74 . . 161 87 87 TRP H H 8.042 . . 162 87 87 TRP HE1 H 9.69 . . 163 87 87 TRP N N 122.616 . . 164 87 87 TRP NE1 N 127.705 . . 165 88 88 LYS H H 8.196 . . 166 88 88 LYS N N 119.346 . . 167 89 89 LYS H H 7.188 . . 168 89 89 LYS N N 118.499 . . 169 90 90 LYS H H 7.376 . . 170 90 90 LYS N N 117.585 . . 171 91 91 GLY H H 7.804 . . 172 91 91 GLY N N 108.981 . . 173 92 92 TRP HE1 H 10.402 . . 174 92 92 TRP NE1 N 129.522 . . 175 96 96 ASN H H 7.984 . . 176 96 96 ASN N N 118.266 . . 177 97 97 ARG H H 8.22 . . 178 97 97 ARG N N 114.469 . . 179 98 98 THR H H 8.176 . . 180 98 98 THR N N 113.526 . . 181 100 100 VAL H H 7.763 . . 182 100 100 VAL N N 120.969 . . 183 101 101 LYS H H 8.365 . . 184 101 101 LYS N N 127.745 . . 185 102 102 ASN H H 9.55 . . 186 102 102 ASN N N 116.647 . . 187 103 103 VAL H H 7.749 . . 188 103 103 VAL N N 118.824 . . 189 104 104 ASP H H 8.516 . . 190 104 104 ASP N N 118.658 . . 191 105 105 LEU H H 7.188 . . 192 105 105 LEU N N 118.499 . . 193 106 106 TRP H H 8.21 . . 194 106 106 TRP HE1 H 9.538 . . 195 106 106 TRP N N 120.686 . . 196 106 106 TRP NE1 N 127.973 . . 197 107 107 LYS H H 8.342 . . 198 107 107 LYS N N 115.447 . . 199 108 108 ARG H H 6.989 . . 200 108 108 ARG N N 119.121 . . 201 109 109 LEU H H 8.788 . . 202 109 109 LEU N N 122.669 . . 203 110 110 ASP H H 8.446 . . 204 110 110 ASP N N 117.404 . . 205 111 111 LYS H H 7.853 . . 206 111 111 LYS N N 116.378 . . 207 112 112 ALA H H 8.18 . . 208 112 112 ALA N N 120.909 . . 209 113 113 ALA H H 8.623 . . 210 113 113 ALA N N 117.322 . . 211 114 114 GLN H H 7.413 . . 212 114 114 GLN N N 113.783 . . 213 115 115 LEU H H 7.194 . . 214 115 115 LEU N N 117.731 . . 215 116 116 HIS H H 7.172 . . 216 116 116 HIS N N 115.374 . . 217 117 117 GLN H H 8.3 . . 218 117 117 GLN N N 120.565 . . 219 118 118 ILE H H 8.58 . . 220 118 118 ILE N N 125.129 . . 221 119 119 ASP H H 8.747 . . 222 119 119 ASP N N 129.295 . . 223 120 120 TRP H H 7.998 . . 224 120 120 TRP HE1 H 10.283 . . 225 120 120 TRP N N 126.244 . . 226 120 120 TRP NE1 N 129.553 . . 227 121 121 ARG H H 8.868 . . 228 121 121 ARG N N 125.129 . . 229 122 122 TRP H H 8.041 . . 230 122 122 TRP HE1 H 9.848 . . 231 122 122 TRP N N 123.051 . . 232 122 122 TRP NE1 N 128.122 . . 233 123 123 VAL H H 8.157 . . 234 123 123 VAL N N 117.843 . . 235 124 124 LYS H H 8.455 . . 236 124 124 LYS N N 121.819 . . 237 125 125 GLY H H 8.383 . . 238 125 125 GLY N N 110.549 . . 239 126 126 HIS H H 8.363 . . 240 126 126 HIS N N 115.269 . . 241 127 127 ALA H H 8.127 . . 242 127 127 ALA N N 123.329 . . 243 128 128 GLY H H 8.829 . . 244 128 128 GLY N N 109.878 . . 245 129 129 HIS H H 8.041 . . 246 129 129 HIS N N 119.296 . . 247 130 130 ALA H H 8.822 . . 248 130 130 ALA N N 126.875 . . 249 131 131 GLU H H 9.626 . . 250 131 131 GLU N N 118.169 . . 251 132 132 ASN H H 7.621 . . 252 132 132 ASN N N 117.106 . . 253 133 133 GLU H H 8.345 . . 254 133 133 GLU N N 121.382 . . 255 134 134 ARG H H 7.954 . . 256 134 134 ARG N N 120.866 . . 257 135 135 CYS H H 8.187 . . 258 135 135 CYS N N 117.573 . . 259 136 136 ASP H H 7.824 . . 260 136 136 ASP N N 120.415 . . 261 137 137 GLN H H 7.888 . . 262 137 137 GLN N N 118.879 . . 263 138 138 LEU H H 8.504 . . 264 138 138 LEU N N 119.848 . . 265 139 139 ALA H H 8.404 . . 266 139 139 ALA N N 123.238 . . 267 140 140 ARG H H 8.267 . . 268 140 140 ARG N N 116.629 . . 269 141 141 ALA H H 7.951 . . 270 141 141 ALA N N 119.388 . . 271 142 142 ALA H H 7.588 . . 272 142 142 ALA N N 119.222 . . 273 143 143 ALA H H 7.82 . . 274 143 143 ALA N N 120.074 . . 275 144 144 GLU H H 7.812 . . 276 144 144 GLU N N 112.077 . . 277 145 145 ALA H H 7.045 . . 278 145 145 ALA N N 123.434 . . 279 146 146 ASN H H 8.021 . . 280 146 146 ASN N N 115.482 . . 281 148 148 THR H H 8.219 . . 282 148 148 THR N N 109.294 . . 283 149 149 GLN H H 7.806 . . 284 149 149 GLN N N 124.462 . . 285 150 150 ILE H H 8.684 . . 286 150 150 ILE N N 126.452 . . 287 151 151 ASP H H 9.598 . . 288 151 151 ASP N N 126.585 . . 289 152 152 THR H H 8.323 . . 290 152 152 THR N N 118.177 . . 291 153 153 GLY H H 7.871 . . 292 153 153 GLY N N 107.023 . . 293 154 154 TYR H H 7.38 . . 294 154 154 TYR N N 121.743 . . 295 155 155 GLN H H 7.62 . . 296 155 155 GLN N N 126.448 . . 297 156 156 ALA H H 8.066 . . 298 156 156 ALA N N 125.268 . . 299 157 157 GLU H H 8.281 . . 300 157 157 GLU N N 120.031 . . 301 158 158 SER H H 7.888 . . 302 158 158 SER N N 122.021 . . stop_ save_