data_10018 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H Chemical Shift Assignments for LINE RNA ; _BMRB_accession_number 10018 _BMRB_flat_file_name bmr10018.str _Entry_type original _Submission_date 2005-12-14 _Accession_date 2005-12-14 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Nomura Yusuke . . 2 Baba Seiki . . 3 Nakazato Shinta . . 4 Sakamoto Taiichi . . 5 Kajikawa Masaki . . 6 Okada Norihiro . . 7 Kawai Gota . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 178 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2006-11-21 original author . stop_ _Original_release_date 2006-11-21 save_ ############################# # Citation for this entry # ############################# save_citiation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution structure and functional importance of a conserved RNA hairpin of eel LINE UnaL2' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17000640 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Nomura Yusuke . . 2 Kajikawa Masaki . . 3 Baba Seiki . . 4 Nakazato Shinta . . 5 Imai T. . . 6 Sakamoto Taiichi . . 7 Okada Norihiro . . 8 Kawai Gota . . stop_ _Journal_abbreviation 'Nucleic Acids Res.' _Journal_volume 34 _Journal_issue 18 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 5184 _Page_last 5193 _Year 2006 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'RNA hairpin of eel LINE' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'RNA hairpin of eel LINE' $LINE36 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state 'RNA single strand' _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_LINE36 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common LINE36 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 36 _Mol_residue_sequence ; GGUUGUACGUCGCUUUGGAU AAAAGCGUCUGCGACC ; loop_ _Residue_seq_code _Residue_label 1 G 2 G 3 U 4 U 5 G 6 U 7 A 8 C 9 G 10 U 11 C 12 G 13 C 14 U 15 U 16 U 17 G 18 G 19 A 20 U 21 A 22 A 23 A 24 A 25 G 26 C 27 G 28 U 29 C 30 U 31 G 32 C 33 G 34 A 35 C 36 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $LINE36 'Japanese eel' 7937 Eukaryota Metazoa Anguilla Japonica stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $LINE36 'enzymatic synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $LINE36 1.0 mM . stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_600MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ save_500MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_1 save_ save_2D_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _Sample_label $sample_1 save_ save_DQF-COSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _Sample_label $sample_1 save_ save_HP-COSY_4 _Saveframe_category NMR_applied_experiment _Experiment_name HP-COSY _Sample_label $sample_1 save_ save_HCP_5 _Saveframe_category NMR_applied_experiment _Experiment_name HCP _Sample_label $sample_1 save_ save_HALF_FILTERED_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name 'HALF FILTERED NOESY' _Sample_label $sample_1 save_ save_2D_HNN-COSY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D HNN-COSY' _Sample_label $sample_1 save_ save_2D_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D HSQC' _Sample_label $sample_1 save_ save_2D_TROSY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TROSY' _Sample_label $sample_1 save_ save_2D_NOESY _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_TOCSY _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_DQF-COSY _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _BMRB_pulse_sequence_accession_number . _Details . save_ save_HP-COSY _Saveframe_category NMR_applied_experiment _Experiment_name HP-COSY _BMRB_pulse_sequence_accession_number . _Details . save_ save_HCP _Saveframe_category NMR_applied_experiment _Experiment_name HCP _BMRB_pulse_sequence_accession_number . _Details . save_ save_HALF_FILTERED_NOESY _Saveframe_category NMR_applied_experiment _Experiment_name 'HALF FILTERED NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_HNN-COSY _Saveframe_category NMR_applied_experiment _Experiment_name '2D HNN-COSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_HSQC _Saveframe_category NMR_applied_experiment _Experiment_name '2D HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_TROSY _Saveframe_category NMR_applied_experiment _Experiment_name '2D TROSY' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_condition_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.0 0.1 pH temperature 293 0.1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_Chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_L36 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D NOESY' '2D TOCSY' DQF-COSY HP-COSY HCP 'HALF FILTERED NOESY' '2D HNN-COSY' '2D HSQC' '2D TROSY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $condition_1 _Chem_shift_reference_set_label $Chem_shift_reference_1 _Mol_system_component_name 'RNA hairpin of eel LINE' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 G H8 H 8.145 0.02 1 2 1 1 G H1' H 5.822 0.02 1 3 1 1 G H2' H 4.957 0.02 1 4 1 1 G H3' H 4.699 0.02 1 5 2 2 G H8 H 7.542 0.02 1 6 2 2 G H1' H 5.936 0.02 1 7 2 2 G H2' H 4.569 0.02 1 8 3 3 U H5 H 5.111 0.02 1 9 3 3 U H6 H 7.641 0.02 1 10 3 3 U H1' H 5.581 0.02 1 11 3 3 U H2' H 4.667 0.02 1 12 3 3 U H3' H 4.376 0.02 1 13 3 3 U H4' H 4.461 0.02 1 14 4 4 U H5 H 5.788 0.02 1 15 4 4 U H6 H 7.925 0.02 1 16 4 4 U H1' H 5.537 0.02 1 17 4 4 U H2' H 4.401 0.02 1 18 5 5 G H8 H 7.763 0.02 1 19 5 5 G H1' H 5.656 0.02 1 20 5 5 G H2' H 4.64 0.02 1 21 5 5 G H3' H 4.311 0.02 1 22 5 5 G H4' H 4.42 0.02 1 23 6 6 U H5 H 5.411 0.02 1 24 6 6 U H6 H 7.608 0.02 1 25 6 6 U H1' H 5.25 0.02 1 26 6 6 U H2' H 3.95 0.02 1 27 6 6 U H3' H 4.5 0.02 1 28 6 6 U H4' H 4.328 0.02 1 29 7 7 A H2 H 6.986 0.02 1 30 7 7 A H8 H 8.308 0.02 1 31 7 7 A H1' H 5.945 0.02 1 32 7 7 A H2' H 4.656 0.02 1 33 7 7 A H3' H 4.765 0.02 1 34 7 7 A H4' H 4.494 0.02 1 35 8 8 C H5 H 5.942 0.02 1 36 8 8 C H6 H 7.831 0.02 1 37 8 8 C H1' H 5.911 0.02 1 38 8 8 C H2' H 4.454 0.02 1 39 8 8 C H3' H 4.609 0.02 1 40 9 9 G H8 H 7.482 0.02 1 41 9 9 G H1' H 5.598 0.02 1 42 9 9 G H2' H 4.864 0.02 1 43 9 9 G H3' H 4.102 0.02 1 44 9 9 G H4' H 4.488 0.02 1 45 10 10 U H5 H 5.039 0.02 1 46 10 10 U H6 H 7.533 0.02 1 47 10 10 U H1' H 5.451 0.02 1 48 10 10 U H2' H 4.183 0.02 1 49 10 10 U H3' H 4.471 0.02 1 50 10 10 U H4' H 4.373 0.02 1 51 11 11 C H5 H 5.779 0.02 1 52 11 11 C H6 H 7.975 0.02 1 53 11 11 C H1' H 5.728 0.02 1 54 11 11 C H2' H 4.553 0.02 1 55 11 11 C H3' H 4.629 0.02 1 56 11 11 C H4' H 4.374 0.02 1 57 12 12 G H8 H 7.616 0.02 1 58 12 12 G H1' H 5.698 0.02 1 59 12 12 G H2' H 4.586 0.02 1 60 12 12 G H3' H 4.437 0.02 1 61 12 12 G H4' H 4.508 0.02 1 62 13 13 C H5 H 5.181 0.02 1 63 13 13 C H6 H 7.735 0.02 1 64 13 13 C H1' H 5.524 0.02 1 65 13 13 C H2' H 4.431 0.02 1 66 14 14 U H5 H 5.408 0.02 1 67 14 14 U H6 H 7.895 0.02 1 68 14 14 U H1' H 5.523 0.02 1 69 14 14 U H2' H 4.421 0.02 1 70 15 15 U H5 H 5.582 0.02 1 71 15 15 U H6 H 7.988 0.02 1 72 15 15 U H1' H 5.631 0.02 1 73 15 15 U H2' H 4.245 0.02 1 74 15 15 U H3' H 4.556 0.02 1 75 16 16 U H5 H 5.376 0.02 1 76 16 16 U H6 H 7.633 0.02 1 77 16 16 U H1' H 5.632 0.02 1 78 16 16 U H2' H 4.31 0.02 1 79 16 16 U H3' H 4.423 0.02 1 80 16 16 U H4' H 4.353 0.02 1 81 17 17 G H8 H 7.664 0.02 1 82 17 17 G H1' H 5.445 0.02 1 83 17 17 G H2' H 4.357 0.02 1 84 17 17 G H3' H 4.663 0.02 1 85 18 18 G H8 H 7.663 0.02 1 86 18 18 G H1' H 5.341 0.02 1 87 18 18 G H2' H 4.385 0.02 1 88 18 18 G H3' H 4.414 0.02 1 89 18 18 G H4' H 4.045 0.02 1 90 19 19 A H2 H 7.917 0.02 1 91 19 19 A H8 H 8.097 0.02 1 92 19 19 A H1' H 5.856 0.02 1 93 19 19 A H2' H 4.671 0.02 1 94 19 19 A H3' H 4.739 0.02 1 95 19 19 A H4' H 4.384 0.02 1 96 20 20 U H5 H 5.825 0.02 1 97 20 20 U H6 H 7.804 0.02 1 98 20 20 U H1' H 5.914 0.02 1 99 20 20 U H2' H 4.407 0.02 1 100 20 20 U H3' H 4.693 0.02 1 101 20 20 U H4' H 4.466 0.02 1 102 20 20 U H5' H 4.194 0.02 1 103 21 21 A H2 H 7.956 0.02 1 104 21 21 A H8 H 7.925 0.02 1 105 21 21 A H1' H 5.834 0.02 1 106 21 21 A H2' H 4.869 0.02 1 107 21 21 A H3' H 4.555 0.02 1 108 22 22 A H2 H 7.131 0.02 1 109 22 22 A H8 H 7.896 0.02 1 110 22 22 A H1' H 4.98 0.02 1 111 22 22 A H2' H 4.516 0.02 1 112 22 22 A H3' H 4.46 0.02 1 113 23 23 A H2 H 7.12 0.02 1 114 23 23 A H8 H 7.593 0.02 1 115 23 23 A H1' H 5.771 0.02 1 116 23 23 A H2' H 4.474 0.02 1 117 24 24 A H2 H 7.367 0.02 1 118 24 24 A H8 H 7.65 0.02 1 119 24 24 A H1' H 5.82 0.02 1 120 24 24 A H2' H 4.514 0.02 1 121 24 24 A H3' H 4.573 0.02 1 122 25 25 G H8 H 7.083 0.02 1 123 25 25 G H1' H 5.528 0.02 1 124 25 25 G H2' H 4.327 0.02 1 125 26 26 C H5 H 5.051 0.02 1 126 26 26 C H6 H 7.461 0.02 1 127 26 26 C H1' H 5.476 0.02 1 128 26 26 C H2' H 4.627 0.02 1 129 26 26 C H3' H 4.424 0.02 1 130 26 26 C H4' H 4.333 0.02 1 131 27 27 G H8 H 7.409 0.02 1 132 27 27 G H1' H 5.674 0.02 1 133 27 27 G H2' H 4.575 0.02 1 134 27 27 G H3' H 4.184 0.02 1 135 27 27 G H4' H 4.475 0.02 1 136 28 28 U H5 H 5.138 0.02 1 137 28 28 U H6 H 7.532 0.02 1 138 28 28 U H1' H 5.414 0.02 1 139 28 28 U H2' H 4.218 0.02 1 140 28 28 U H3' H 4.391 0.02 1 141 28 28 U H4' H 4.364 0.02 1 142 29 29 C H5 H 5.698 0.02 1 143 29 29 C H6 H 7.984 0.02 1 144 29 29 C H1' H 5.612 0.02 1 145 29 29 C H2' H 4.378 0.02 1 146 29 29 C H3' H 4.468 0.02 1 147 30 30 U H5 H 5.555 0.02 1 148 30 30 U H6 H 7.963 0.02 1 149 30 30 U H1' H 5.585 0.02 1 150 30 30 U H2' H 4.548 0.02 1 151 30 30 U H3' H 4.607 0.02 1 152 30 30 U H4' H 4.436 0.02 1 153 31 31 G H8 H 7.786 0.02 1 154 31 31 G H1' H 5.808 0.02 1 155 31 31 G H2' H 4.47 0.02 1 156 31 31 G H3' H 4.543 0.02 1 157 32 32 C H5 H 5.397 0.02 1 158 32 32 C H6 H 7.586 0.02 1 159 32 32 C H1' H 5.419 0.02 1 160 32 32 C H2' H 4.316 0.02 1 161 32 32 C H3' H 4.481 0.02 1 162 33 33 G H8 H 7.383 0.02 1 163 33 33 G H1' H 5.613 0.02 1 164 33 33 G H2' H 4.747 0.02 1 165 34 34 A H2 H 7.862 0.02 1 166 34 34 A H8 H 7.884 0.02 1 167 34 34 A H1' H 5.914 0.02 1 168 34 34 A H2' H 4.454 0.02 1 169 35 35 C H5 H 5.146 0.02 1 170 35 35 C H6 H 7.478 0.02 1 171 35 35 C H1' H 5.41 0.02 1 172 35 35 C H2' H 4.117 0.02 1 173 35 35 C H4' H 4.351 0.02 1 174 36 36 C H5 H 5.395 0.02 1 175 36 36 C H6 H 7.626 0.02 1 176 36 36 C H1' H 5.747 0.02 1 177 36 36 C H2' H 3.985 0.02 1 178 36 36 C H3' H 4.14 0.02 1 stop_ save_