data_11429 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HMGB2 ; _BMRB_accession_number 11429 _BMRB_flat_file_name bmr11429.str _Entry_type original _Submission_date 2011-02-22 _Accession_date 2011-02-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 KURITA J. . . 2 UEWAKI J. . . 3 SHIMAHARA H. . . 4 YOSHIDA M. . . 5 TATE S. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 459 "13C chemical shifts" 220 "15N chemical shifts" 77 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-06-05 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 11430 'SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF THE HMGB2' 11431 'SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF THE HMGB2 (H22Y)' stop_ _Original_release_date 2012-06-05 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'STRUCUTRAL COMPARISON OF TWO HMG-BOXES IN THE NON-HISTONE PROTEIN HMG-2 WITH IN THE HMG-1' _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 KURITA J. . . 2 UEWAKI J. . . 3 SHIMAHARA H. . . 4 YOSHIDA M. . . 5 TATE S. . . stop_ _Journal_abbreviation . _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'HMGB2 A-domain' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label HMG2A $HMG2A stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic yes _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_HMG2A _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common HMG2A _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 77 _Mol_residue_sequence ; MGKGDPNKPRGKMSSYAFFV QTSREEHKKKHPDSSVNFAE FSKKCSERWKTMSAKEKSKF EDMAKSDKARYDREMKN ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLY 3 LYS 4 GLY 5 ASP 6 PRO 7 ASN 8 LYS 9 PRO 10 ARG 11 GLY 12 LYS 13 MET 14 SER 15 SER 16 TYR 17 ALA 18 PHE 19 PHE 20 VAL 21 GLN 22 THR 23 SER 24 ARG 25 GLU 26 GLU 27 HIS 28 LYS 29 LYS 30 LYS 31 HIS 32 PRO 33 ASP 34 SER 35 SER 36 VAL 37 ASN 38 PHE 39 ALA 40 GLU 41 PHE 42 SER 43 LYS 44 LYS 45 CYS 46 SER 47 GLU 48 ARG 49 TRP 50 LYS 51 THR 52 MET 53 SER 54 ALA 55 LYS 56 GLU 57 LYS 58 SER 59 LYS 60 PHE 61 GLU 62 ASP 63 MET 64 ALA 65 LYS 66 SER 67 ASP 68 LYS 69 ALA 70 ARG 71 TYR 72 ASP 73 ARG 74 GLU 75 MET 76 LYS 77 ASN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1J3X "Solution Structure Of The N-Terminal Domain Of The Hmgb2" 100.00 77 100.00 100.00 4.52e-47 REF XP_006040222 "PREDICTED: high mobility group protein B2-like, partial [Bubalus bubalis]" 100.00 165 98.70 98.70 4.47e-45 REF XP_007083590 "PREDICTED: high mobility group protein B2-like [Panthera tigris altaica]" 98.70 325 98.68 98.68 2.57e-43 REF XP_008582451 "PREDICTED: high mobility group protein B2 isoform X1 [Galeopterus variegatus]" 100.00 217 98.70 98.70 4.53e-45 REF XP_008582452 "PREDICTED: high mobility group protein B2 isoform X1 [Galeopterus variegatus]" 100.00 217 98.70 98.70 4.53e-45 REF XP_008582454 "PREDICTED: high mobility group protein B2 isoform X2 [Galeopterus variegatus]" 100.00 207 98.70 98.70 5.36e-45 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $HMG2A pig 9823 Eukaryota Metazoa Sus scrofa stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $HMG2A 'recombinant technology' 'E. coli' Escherichia coli . pET22b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $HMG2A 1 mM '[U-13C; U-15N]' H2O 95 % . D2O 5 % . 'sodium acetate' 50 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 750 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_1H-15N_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_1H-13C_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 50 . mM pH 5.0 . pH pressure 1 . atm temperature 293 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D 1H-15N NOESY' '3D 1H-13C NOESY' '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name HMG2A _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 MET HA H 4.29 . 1 2 1 1 MET HB2 H 1.71 . . 3 1 1 MET HB3 H 1.86 . . 4 1 1 MET C C 177.19 . 1 5 1 1 MET CA C 56.36 . 1 6 1 1 MET CB C 30.85 . 1 7 2 2 GLY H H 8.50 . 1 8 2 2 GLY HA2 H 3.84 . . 9 2 2 GLY HA3 H 3.98 . . 10 2 2 GLY C C 173.71 . 1 11 2 2 GLY CA C 45.18 . 1 12 2 2 GLY N N 110.36 . 1 13 3 3 LYS H H 8.12 . 1 14 3 3 LYS HA H 4.25 . 1 15 3 3 LYS HB2 H 1.63 . . 16 3 3 LYS HB3 H 1.74 . . 17 3 3 LYS HD2 H 1.63 . 2 18 3 3 LYS HD3 H 1.63 . 2 19 3 3 LYS HE2 H 2.78 . . 20 3 3 LYS HE3 H 2.92 . . 21 3 3 LYS HG2 H 1.22 . . 22 3 3 LYS HG3 H 1.28 . . 23 3 3 LYS C C 177.26 . 1 24 3 3 LYS CA C 57.13 . 1 25 3 3 LYS CB C 32.78 . 1 26 3 3 LYS N N 120.76 . 1 27 4 4 GLY H H 8.67 . 1 28 4 4 GLY HA2 H 3.92 . . 29 4 4 GLY C C 173.58 . 1 30 4 4 GLY N N 110.94 . 1 31 5 5 ASP H H 8.13 . 1 32 5 5 ASP HA H 4.82 . 1 33 5 5 ASP HB2 H 2.59 . . 34 5 5 ASP HB3 H 2.75 . . 35 5 5 ASP CA C 52.21 . 1 36 5 5 ASP CB C 41.07 . 1 37 5 5 ASP N N 121.75 . 1 38 6 6 PRO HA H 4.43 . 1 39 6 6 PRO HB2 H 1.94 . . 40 6 6 PRO HB3 H 2.27 . . 41 6 6 PRO HD2 H 3.84 . . 42 6 6 PRO HD3 H 3.89 . . 43 6 6 PRO HG2 H 1.96 . . 44 6 6 PRO HG3 H 2.05 . . 45 6 6 PRO C C 176.93 . 1 46 6 6 PRO CA C 63.56 . 1 47 6 6 PRO CB C 32.15 . 1 48 7 7 ASN H H 8.54 . 1 49 7 7 ASN HA H 4.67 . 1 50 7 7 ASN HB2 H 2.74 . . 51 7 7 ASN HB3 H 2.80 . . 52 7 7 ASN HD21 H 6.99 . . 53 7 7 ASN HD22 H 7.78 . . 54 7 7 ASN C C 175.52 . 1 55 7 7 ASN CA C 53.32 . 1 56 7 7 ASN CB C 38.95 . 1 57 7 7 ASN N N 118.06 . 1 58 7 7 ASN ND2 N 114.23 . 1 59 8 8 LYS H H 7.94 . 1 60 8 8 LYS HA H 4.60 . 1 61 8 8 LYS HB2 H 1.71 . . 62 8 8 LYS HB3 H 1.82 . . 63 8 8 LYS HD2 H 1.70 . 2 64 8 8 LYS HD3 H 1.70 . 2 65 8 8 LYS HE2 H 3.01 . 2 66 8 8 LYS HE3 H 3.01 . 2 67 8 8 LYS HG2 H 1.82 . 2 68 8 8 LYS HG3 H 1.82 . 2 69 8 8 LYS CA C 54.24 . 1 70 8 8 LYS CB C 32.74 . 1 71 8 8 LYS N N 122.50 . 1 72 9 9 PRO HA H 4.42 . 1 73 9 9 PRO HB2 H 1.84 . . 74 9 9 PRO HB3 H 2.32 . . 75 9 9 PRO HD2 H 3.58 . . 76 9 9 PRO HD3 H 3.81 . . 77 9 9 PRO C C 176.83 . 1 78 9 9 PRO CA C 63.02 . 1 79 9 9 PRO CB C 32.08 . 1 80 10 10 ARG H H 8.65 . 1 81 10 10 ARG HA H 4.29 . 1 82 10 10 ARG HB2 H 1.73 . . 83 10 10 ARG HB3 H 1.81 . . 84 10 10 ARG HD2 H 3.19 . 2 85 10 10 ARG HD3 H 3.19 . 2 86 10 10 ARG HG2 H 1.70 . 2 87 10 10 ARG HG3 H 1.70 . 2 88 10 10 ARG C C 177.20 . 1 89 10 10 ARG CA C 56.36 . 1 90 10 10 ARG CB C 30.83 . 1 91 10 10 ARG N N 122.38 . 1 92 11 11 GLY H H 8.52 . 1 93 11 11 GLY HA2 H 3.92 . . 94 11 11 GLY C C 176.95 . 1 95 11 11 GLY CA C 45.16 . 1 96 11 11 GLY N N 110.24 . 1 97 12 12 LYS H H 8.50 . 1 98 12 12 LYS HA H 4.29 . 1 99 12 12 LYS HB2 H 1.76 . . 100 12 12 LYS HB3 H 1.86 . . 101 12 12 LYS HD2 H 1.49 . 2 102 12 12 LYS HD3 H 1.49 . 2 103 12 12 LYS HE2 H 3.01 . 2 104 12 12 LYS HE3 H 3.01 . 2 105 12 12 LYS HG2 H 1.45 . 2 106 12 12 LYS HG3 H 1.45 . 2 107 12 12 LYS C C 176.90 . 1 108 12 12 LYS CA C 56.61 . 1 109 12 12 LYS CB C 32.86 . 1 110 12 12 LYS N N 121.40 . 1 111 13 13 MET H H 8.75 . 1 112 13 13 MET HA H 4.45 . 1 113 13 13 MET HB2 H 1.71 . . 114 13 13 MET HB3 H 1.81 . . 115 13 13 MET HE H 1.71 . 1 116 13 13 MET HG2 H 2.29 . . 117 13 13 MET HG3 H 2.35 . . 118 13 13 MET C C 175.53 . 1 119 13 13 MET CA C 55.15 . 1 120 13 13 MET CB C 34.28 . 1 121 13 13 MET N N 123.48 . 1 122 14 14 SER H H 8.14 . 1 123 14 14 SER HA H 4.64 . 1 124 14 14 SER HB2 H 4.11 . . 125 14 14 SER HB3 H 4.51 . . 126 14 14 SER C C 176.83 . 1 127 14 14 SER CA C 57.26 . 1 128 14 14 SER CB C 65.45 . 1 129 14 14 SER N N 118.42 . 1 130 15 15 SER H H 9.26 . 1 131 15 15 SER HA H 4.35 . 1 132 15 15 SER C C 176.17 . 1 133 15 15 SER CA C 61.50 . 1 134 15 15 SER CB C 62.37 . 1 135 15 15 SER N N 117.24 . 1 136 16 16 TYR H H 8.03 . 1 137 16 16 TYR HA H 4.64 . 1 138 16 16 TYR HB2 H 2.75 . . 139 16 16 TYR HB3 H 2.82 . . 140 16 16 TYR HD1 H 6.42 . 3 141 16 16 TYR HD2 H 6.42 . 3 142 16 16 TYR HE1 H 5.90 . 3 143 16 16 TYR HE2 H 5.90 . 3 144 16 16 TYR C C 174.81 . 1 145 16 16 TYR CA C 53.53 . 1 146 16 16 TYR CB C 39.04 . 1 147 16 16 TYR N N 122.34 . 1 148 17 17 ALA H H 7.94 . 1 149 17 17 ALA HA H 3.71 . 1 150 17 17 ALA HB H 1.43 . 1 151 17 17 ALA C C 169.54 . 1 152 17 17 ALA CA C 55.22 . 1 153 17 17 ALA CB C 18.03 . 1 154 17 17 ALA N N 121.87 . 1 155 18 18 PHE H H 8.28 . 1 156 18 18 PHE HA H 4.23 . 1 157 18 18 PHE HB2 H 3.38 . . 158 18 18 PHE HB3 H 3.63 . . 159 18 18 PHE HD1 H 7.37 . 3 160 18 18 PHE HD2 H 7.37 . 3 161 18 18 PHE HE1 H 7.37 . 3 162 18 18 PHE HE2 H 7.25 . . 163 18 18 PHE C C 176.89 . 1 164 18 18 PHE CA C 63.16 . 1 165 18 18 PHE CB C 39.77 . 1 166 18 18 PHE N N 118.50 . 1 167 19 19 PHE H H 8.83 . 1 168 19 19 PHE HA H 4.27 . 1 169 19 19 PHE HB2 H 3.42 . . 170 19 19 PHE HB3 H 3.66 . . 171 19 19 PHE C C 177.53 . 1 172 19 19 PHE CA C 63.34 . 1 173 19 19 PHE CB C 40.50 . 1 174 19 19 PHE N N 125.21 . 1 175 20 20 VAL H H 7.98 . 1 176 20 20 VAL HA H 3.10 . 1 177 20 20 VAL HB H 1.76 . 1 178 20 20 VAL HG1 H 0.27 . . 179 20 20 VAL HG2 H 0.87 . . 180 20 20 VAL C C 177.62 . 1 181 20 20 VAL CA C 66.99 . 1 182 20 20 VAL CB C 31.38 . 1 183 20 20 VAL N N 120.36 . 1 184 21 21 GLN H H 7.97 . 1 185 21 21 GLN HA H 3.93 . 1 186 21 21 GLN HB2 H 2.12 . . 187 21 21 GLN HE21 H 6.80 . . 188 21 21 GLN HE22 H 7.74 . . 189 21 21 GLN HG2 H 2.33 . 2 190 21 21 GLN HG3 H 2.33 . 2 191 21 21 GLN C C 178.81 . 1 192 21 21 GLN CA C 59.52 . 1 193 21 21 GLN CB C 29.24 . 1 194 21 21 GLN N N 118.93 . 1 195 21 21 GLN NE2 N 111.80 . 1 196 22 22 THR H H 8.52 . 1 197 22 22 THR HA H 4.09 . 1 198 22 22 THR HB H 4.32 . 1 199 22 22 THR HG2 H 1.55 . 1 200 22 22 THR C C 177.00 . 1 201 22 22 THR CA C 66.54 . 1 202 22 22 THR CB C 69.23 . 1 203 22 22 THR N N 115.11 . 1 204 23 23 SER H H 8.26 . 1 205 23 23 SER HA H 4.29 . 1 206 23 23 SER HB2 H 3.93 . . 207 23 23 SER C C 178.86 . 1 208 23 23 SER CA C 62.44 . 1 209 23 23 SER CB C 64.23 . 1 210 23 23 SER N N 119.01 . 1 211 24 24 ARG H H 8.39 . 1 212 24 24 ARG HA H 4.22 . 1 213 24 24 ARG HB2 H 1.42 . . 214 24 24 ARG HB3 H 1.73 . . 215 24 24 ARG C C 179.46 . 1 216 24 24 ARG CA C 66.94 . 1 217 24 24 ARG CB C 31.24 . 1 218 24 24 ARG N N 124.74 . 1 219 25 25 GLU H H 8.18 . 1 220 25 25 GLU HA H 3.73 . 1 221 25 25 GLU HB2 H 2.18 . . 222 25 25 GLU HB3 H 2.61 . . 223 25 25 GLU C C 178.27 . 1 224 25 25 GLU CA C 60.76 . 1 225 25 25 GLU CB C 38.65 . 1 226 25 25 GLU N N 125.71 . 1 227 26 26 GLU H H 8.32 . 1 228 26 26 GLU HA H 4.07 . 1 229 26 26 GLU HB2 H 2.13 . . 230 26 26 GLU HB3 H 2.37 . . 231 26 26 GLU HG2 H 2.37 . . 232 26 26 GLU HG3 H 2.50 . . 233 26 26 GLU C C 178.69 . 1 234 26 26 GLU CA C 59.17 . 1 235 26 26 GLU CB C 29.48 . 1 236 26 26 GLU N N 119.92 . 1 237 27 27 HIS H H 8.24 . 1 238 27 27 HIS HA H 4.30 . 1 239 27 27 HIS HB2 H 3.14 . . 240 27 27 HIS HB3 H 3.32 . . 241 27 27 HIS HD2 H 7.02 . 1 242 27 27 HIS HE1 H 8.00 . 1 243 27 27 HIS C C 176.77 . 1 244 27 27 HIS CA C 60.15 . 1 245 27 27 HIS CB C 29.33 . 1 246 27 27 HIS N N 119.14 . 1 247 28 28 LYS H H 7.95 . 1 248 28 28 LYS HA H 3.96 . 1 249 28 28 LYS HB2 H 1.88 . . 250 28 28 LYS HD2 H 1.76 . 2 251 28 28 LYS HD3 H 1.76 . 2 252 28 28 LYS HE2 H 2.98 . 2 253 28 28 LYS HE3 H 2.98 . 2 254 28 28 LYS HG2 H 1.42 . . 255 28 28 LYS HG3 H 1.53 . . 256 28 28 LYS C C 177.97 . 1 257 28 28 LYS CA C 58.37 . 1 258 28 28 LYS CB C 32.24 . 1 259 28 28 LYS N N 118.87 . 1 260 29 29 LYS H H 7.56 . 1 261 29 29 LYS HA H 3.98 . 1 262 29 29 LYS HB2 H 1.88 . . 263 29 29 LYS HD2 H 1.71 . 2 264 29 29 LYS HD3 H 1.71 . 2 265 29 29 LYS HE2 H 2.97 . 2 266 29 29 LYS HE3 H 2.97 . 2 267 29 29 LYS HG2 H 1.40 . . 268 29 29 LYS HG3 H 1.56 . . 269 29 29 LYS CA C 58.59 . 1 270 29 29 LYS CB C 32.60 . 1 271 29 29 LYS N N 117.38 . 1 272 30 30 LYS H H 7.56 . 1 273 30 30 LYS HA H 3.99 . 1 274 30 30 LYS HB2 H 1.38 . . 275 30 30 LYS HB3 H 1.56 . . 276 30 30 LYS HD2 H 1.65 . 2 277 30 30 LYS HD3 H 1.65 . 2 278 30 30 LYS HE2 H 2.96 . 2 279 30 30 LYS HE3 H 2.96 . 2 280 30 30 LYS HG2 H 1.15 . . 281 30 30 LYS HG3 H 1.35 . . 282 30 30 LYS C C 176.38 . 1 283 30 30 LYS CA C 57.43 . 1 284 30 30 LYS CB C 33.82 . 1 285 30 30 LYS N N 116.32 . 1 286 31 31 HIS H H 7.88 . 1 287 31 31 HIS HA H 5.02 . 1 288 31 31 HIS HB2 H 2.66 . . 289 31 31 HIS HB3 H 2.97 . . 290 31 31 HIS HD2 H 6.76 . 1 291 31 31 HIS HE1 H 8.60 . 1 292 31 31 HIS CA C 52.29 . 1 293 31 31 HIS CB C 28.19 . 1 294 31 31 HIS N N 115.31 . 1 295 32 32 PRO HA H 4.51 . 1 296 32 32 PRO HB2 H 1.95 . . 297 32 32 PRO HB3 H 2.34 . . 298 32 32 PRO HD2 H 3.32 . . 299 32 32 PRO HD3 H 3.56 . . 300 32 32 PRO HG2 H 1.98 . 2 301 32 32 PRO HG3 H 1.98 . 2 302 32 32 PRO C C 177.31 . 1 303 32 32 PRO CA C 64.79 . 1 304 32 32 PRO CB C 31.89 . 1 305 33 33 ASP H H 8.57 . 1 306 33 33 ASP HA H 4.68 . 1 307 33 33 ASP HB2 H 2.71 . . 308 33 33 ASP C C 175.91 . 1 309 33 33 ASP CA C 53.85 . 1 310 33 33 ASP CB C 40.63 . 1 311 33 33 ASP N N 116.76 . 1 312 34 34 SER H H 7.61 . 1 313 34 34 SER HA H 4.55 . 1 314 34 34 SER HB2 H 3.88 . . 315 34 34 SER C C 174.56 . 1 316 34 34 SER CA C 57.98 . 1 317 34 34 SER CB C 64.33 . 1 318 34 34 SER N N 114.59 . 1 319 35 35 SER H H 8.70 . 1 320 35 35 SER HA H 4.56 . 1 321 35 35 SER HB2 H 3.86 . . 322 35 35 SER C C 175.08 . 1 323 35 35 SER CA C 57.99 . 1 324 35 35 SER CB C 63.92 . 1 325 35 35 SER N N 120.19 . 1 326 36 36 VAL H H 8.31 . 1 327 36 36 VAL HA H 4.00 . 1 328 36 36 VAL HB H 1.81 . 1 329 36 36 VAL HG1 H 0.55 . . 330 36 36 VAL HG2 H 0.68 . . 331 36 36 VAL C C 175.38 . 1 332 36 36 VAL CA C 61.81 . 1 333 36 36 VAL CB C 33.00 . 1 334 36 36 VAL N N 122.75 . 1 335 37 37 ASN H H 8.72 . 1 336 37 37 ASN HA H 4.74 . 1 337 37 37 ASN HB2 H 2.92 . . 338 37 37 ASN HD21 H 7.02 . . 339 37 37 ASN HD22 H 7.75 . . 340 37 37 ASN C C 175.54 . 1 341 37 37 ASN CA C 52.71 . 1 342 37 37 ASN CB C 38.58 . 1 343 37 37 ASN N N 125.73 . 1 344 37 37 ASN ND2 N 113.10 . 1 345 38 38 PHE H H 8.98 . 1 346 38 38 PHE HA H 4.13 . 1 347 38 38 PHE HB2 H 3.19 . . 348 38 38 PHE HB3 H 3.23 . . 349 38 38 PHE HD1 H 7.18 . 3 350 38 38 PHE HD2 H 7.18 . 3 351 38 38 PHE HE1 H 7.25 . 3 352 38 38 PHE HE2 H 7.25 . 3 353 38 38 PHE HZ H 7.25 . 1 354 38 38 PHE C C 177.48 . 1 355 38 38 PHE CA C 62.15 . 1 356 38 38 PHE CB C 39.11 . 1 357 38 38 PHE N N 125.72 . 1 358 39 39 ALA H H 8.62 . 1 359 39 39 ALA HA H 4.10 . 1 360 39 39 ALA HB H 1.52 . 1 361 39 39 ALA C C 180.69 . 1 362 39 39 ALA CA C 55.52 . 1 363 39 39 ALA CB C 17.85 . 1 364 39 39 ALA N N 123.79 . 1 365 40 40 GLU H H 8.02 . 1 366 40 40 GLU HA H 4.03 . 1 367 40 40 GLU HB2 H 2.05 . . 368 40 40 GLU HG2 H 2.24 . . 369 40 40 GLU HG3 H 2.35 . . 370 40 40 GLU C C 178.85 . 1 371 40 40 GLU CA C 58.74 . 1 372 40 40 GLU CB C 29.65 . 1 373 40 40 GLU N N 119.24 . 1 374 41 41 PHE H H 8.62 . 1 375 41 41 PHE HA H 4.01 . 1 376 41 41 PHE HB2 H 2.79 . . 377 41 41 PHE HB3 H 2.99 . . 378 41 41 PHE HD1 H 6.86 . 3 379 41 41 PHE HD2 H 6.86 . 3 380 41 41 PHE HE1 H 6.91 . 3 381 41 41 PHE HE2 H 6.91 . 3 382 41 41 PHE HZ H 6.79 . 1 383 41 41 PHE C C 177.87 . 1 384 41 41 PHE CA C 61.80 . 1 385 41 41 PHE CB C 39.75 . 1 386 41 41 PHE N N 122.04 . 1 387 42 42 SER H H 8.56 . 1 388 42 42 SER HA H 3.74 . 1 389 42 42 SER HB2 H 3.67 . . 390 42 42 SER HB3 H 3.73 . . 391 42 42 SER C C 176.13 . 1 392 42 42 SER CA C 62.07 . 1 393 42 42 SER N N 115.54 . 1 394 43 43 LYS H H 7.45 . 1 395 43 43 LYS HA H 4.06 . 1 396 43 43 LYS HB2 H 1.91 . . 397 43 43 LYS HB3 H 1.96 . . 398 43 43 LYS HD2 H 1.64 . 2 399 43 43 LYS HD3 H 1.64 . 2 400 43 43 LYS HE2 H 2.99 . 2 401 43 43 LYS HE3 H 2.99 . 2 402 43 43 LYS HG2 H 1.39 . . 403 43 43 LYS HG3 H 1.52 . . 404 43 43 LYS C C 180.11 . 1 405 43 43 LYS CA C 59.41 . 1 406 43 43 LYS CB C 32.18 . 1 407 43 43 LYS N N 122.40 . 1 408 44 44 LYS H H 7.75 . 1 409 44 44 LYS HA H 4.06 . 1 410 44 44 LYS HB2 H 1.79 . . 411 44 44 LYS HD2 H 1.64 . 2 412 44 44 LYS HD3 H 1.64 . 2 413 44 44 LYS HE2 H 2.99 . 2 414 44 44 LYS HE3 H 2.99 . 2 415 44 44 LYS HG2 H 1.38 . . 416 44 44 LYS HG3 H 1.51 . . 417 44 44 LYS C C 180.11 . 1 418 44 44 LYS CA C 59.25 . 1 419 44 44 LYS CB C 31.75 . 1 420 44 44 LYS N N 119.98 . 1 421 45 45 CYS H H 8.05 . 1 422 45 45 CYS HA H 4.09 . 1 423 45 45 CYS HB2 H 2.51 . . 424 45 45 CYS HB3 H 3.07 . . 425 45 45 CYS C C 176.68 . 1 426 45 45 CYS CA C 64.57 . 1 427 45 45 CYS CB C 27.09 . 1 428 45 45 CYS N N 117.68 . 1 429 46 46 SER H H 8.37 . 1 430 46 46 SER HA H 4.21 . 1 431 46 46 SER HB2 H 4.00 . . 432 46 46 SER HB3 H 4.08 . . 433 46 46 SER C C 177.37 . 1 434 46 46 SER CA C 62.13 . 1 435 46 46 SER CB C 63.70 . 1 436 46 46 SER N N 117.37 . 1 437 47 47 GLU H H 7.91 . 1 438 47 47 GLU HA H 4.08 . 1 439 47 47 GLU HB2 H 2.16 . . 440 47 47 GLU HG2 H 2.34 . . 441 47 47 GLU HG3 H 2.46 . . 442 47 47 GLU C C 179.28 . 1 443 47 47 GLU CA C 58.95 . 1 444 47 47 GLU CB C 29.47 . 1 445 47 47 GLU N N 121.04 . 1 446 48 48 ARG H H 8.12 . 1 447 48 48 ARG HA H 4.14 . 1 448 48 48 ARG HB2 H 2.15 . . 449 48 48 ARG HD2 H 3.30 . 2 450 48 48 ARG HD3 H 3.30 . 2 451 48 48 ARG HG2 H 1.94 . 2 452 48 48 ARG HG3 H 1.94 . 2 453 48 48 ARG C C 179.02 . 1 454 48 48 ARG CA C 58.81 . 1 455 48 48 ARG CB C 30.50 . 1 456 48 48 ARG N N 120.05 . 1 457 49 49 TRP H H 8.76 . 1 458 49 49 TRP HA H 4.38 . 1 459 49 49 TRP HB2 H 3.29 . . 460 49 49 TRP HB3 H 3.40 . . 461 49 49 TRP HD1 H 7.02 . 1 462 49 49 TRP HE1 H 10.20 . 1 463 49 49 TRP HE3 H 6.28 . 1 464 49 49 TRP HH2 H 7.12 . 1 465 49 49 TRP HZ2 H 7.62 . 1 466 49 49 TRP HZ3 H 6.45 . 1 467 49 49 TRP C C 177.97 . 1 468 49 49 TRP CA C 59.67 . 1 469 49 49 TRP CB C 30.60 . 1 470 49 49 TRP N N 120.86 . 1 471 50 50 LYS H H 7.47 . 1 472 50 50 LYS HA H 4.05 . 1 473 50 50 LYS HB2 H 1.71 . . 474 50 50 LYS HB3 H 1.98 . . 475 50 50 LYS HD2 H 1.71 . 2 476 50 50 LYS HD3 H 1.71 . 2 477 50 50 LYS HE2 H 3.01 . 2 478 50 50 LYS HE3 H 3.01 . 2 479 50 50 LYS HG2 H 1.40 . . 480 50 50 LYS HG3 H 1.48 . . 481 50 50 LYS C C 178.15 . 1 482 50 50 LYS CA C 58.88 . 1 483 50 50 LYS CB C 33.04 . 1 484 50 50 LYS N N 114.52 . 1 485 51 51 THR H H 7.41 . 1 486 51 51 THR HA H 4.39 . 1 487 51 51 THR HB H 4.17 . 1 488 51 51 THR HG2 H 1.25 . 1 489 51 51 THR C C 174.88 . 1 490 51 51 THR CA C 61.28 . 1 491 51 51 THR CB C 69.71 . 1 492 51 51 THR N N 106.59 . 1 493 52 52 MET H H 7.14 . 1 494 52 52 MET HA H 4.21 . 1 495 52 52 MET HB2 H 1.59 . . 496 52 52 MET HB3 H 1.80 . . 497 52 52 MET HG2 H 2.52 . 2 498 52 52 MET HG3 H 2.52 . 2 499 52 52 MET C C 175.88 . 1 500 52 52 MET CA C 56.45 . 1 501 52 52 MET CB C 35.15 . 1 502 52 52 MET N N 124.02 . 1 503 53 53 SER H H 9.00 . 1 504 53 53 SER HA H 4.34 . 1 505 53 53 SER HB2 H 4.07 . . 506 53 53 SER C C 174.63 . 1 507 53 53 SER CA C 57.13 . 1 508 53 53 SER CB C 65.46 . 1 509 53 53 SER N N 120.05 . 1 510 54 54 ALA H H 8.89 . 1 511 54 54 ALA HA H 3.92 . 1 512 54 54 ALA HB H 1.43 . 1 513 54 54 ALA C C 181.07 . 1 514 54 54 ALA CA C 55.58 . 1 515 54 54 ALA CB C 17.70 . 1 516 54 54 ALA N N 123.68 . 1 517 55 55 LYS H H 8.31 . 1 518 55 55 LYS HA H 4.07 . 1 519 55 55 LYS HB2 H 1.76 . . 520 55 55 LYS HB3 H 1.86 . . 521 55 55 LYS HD2 H 1.63 . 2 522 55 55 LYS HD3 H 1.63 . 2 523 55 55 LYS HE2 H 4.09 . 2 524 55 55 LYS HE3 H 4.09 . 2 525 55 55 LYS HG2 H 1.44 . 2 526 55 55 LYS HG3 H 1.44 . 2 527 55 55 LYS C C 179.81 . 1 528 55 55 LYS CA C 59.27 . 1 529 55 55 LYS CB C 32.29 . 1 530 55 55 LYS N N 118.21 . 1 531 56 56 GLU H H 7.76 . 1 532 56 56 GLU HA H 4.07 . 1 533 56 56 GLU HB2 H 2.23 . . 534 56 56 GLU HB3 H 2.37 . . 535 56 56 GLU HG2 H 2.52 . 2 536 56 56 GLU HG3 H 2.52 . 2 537 56 56 GLU C C 178.99 . 1 538 56 56 GLU CA C 59.13 . 1 539 56 56 GLU CB C 30.09 . 1 540 56 56 GLU N N 121.02 . 1 541 57 57 LYS H H 8.17 . 1 542 57 57 LYS HA H 4.09 . 1 543 57 57 LYS HB2 H 1.60 . . 544 57 57 LYS HB3 H 1.75 . . 545 57 57 LYS HD2 H 1.24 . 2 546 57 57 LYS HD3 H 1.24 . 2 547 57 57 LYS HE2 H 3.03 . 2 548 57 57 LYS HE3 H 3.03 . 2 549 57 57 LYS HG2 H 0.88 . . 550 57 57 LYS HG3 H 0.98 . . 551 57 57 LYS C C 179.40 . 1 552 57 57 LYS CA C 59.76 . 1 553 57 57 LYS CB C 33.24 . 1 554 57 57 LYS NZ N 117.37 . 1 555 58 58 SER H H 7.85 . 1 556 58 58 SER HA H 4.20 . 1 557 58 58 SER HB2 H 4.01 . . 558 58 58 SER HB3 H 4.07 . . 559 58 58 SER C C 175.91 . 1 560 58 58 SER CA C 62.23 . 1 561 58 58 SER N N 115.32 . 1 562 59 59 LYS H H 7.71 . 1 563 59 59 LYS HA H 4.07 . 1 564 59 59 LYS HB2 H 1.39 . . 565 59 59 LYS HB3 H 1.67 . . 566 59 59 LYS HD2 H 1.52 . 2 567 59 59 LYS HD3 H 1.52 . 2 568 59 59 LYS HE2 H 2.82 . 2 569 59 59 LYS HE3 H 2.82 . 2 570 59 59 LYS HG2 H 0.90 . 2 571 59 59 LYS HG3 H 0.90 . 2 572 59 59 LYS C C 178.40 . 1 573 59 59 LYS CA C 59.00 . 1 574 59 59 LYS CB C 31.74 . 1 575 59 59 LYS N N 120.67 . 1 576 60 60 PHE H H 7.40 . 1 577 60 60 PHE HA H 4.46 . 1 578 60 60 PHE HB2 H 3.37 . . 579 60 60 PHE HD1 H 7.62 . 3 580 60 60 PHE HD2 H 7.62 . 3 581 60 60 PHE HE1 H 7.70 . 3 582 60 60 PHE HE2 H 7.70 . 3 583 60 60 PHE HZ H 7.82 . 1 584 60 60 PHE C C 177.88 . 1 585 60 60 PHE CA C 60.87 . 1 586 60 60 PHE CB C 39.04 . 1 587 60 60 PHE N N 119.39 . 1 588 61 61 GLU H H 8.12 . 1 589 61 61 GLU HA H 4.19 . 1 590 61 61 GLU HB2 H 2.22 . . 591 61 61 GLU HB3 H 2.29 . . 592 61 61 GLU HG2 H 2.51 . 2 593 61 61 GLU HG3 H 2.51 . 2 594 61 61 GLU C C 179.50 . 1 595 61 61 GLU CA C 59.00 . 1 596 61 61 GLU CB C 29.07 . 1 597 61 61 GLU N N 121.49 . 1 598 62 62 ASP H H 8.43 . 1 599 62 62 ASP HA H 4.42 . 1 600 62 62 ASP HB2 H 2.64 . . 601 62 62 ASP HB3 H 2.74 . . 602 62 62 ASP C C 179.05 . 1 603 62 62 ASP CA C 57.21 . 1 604 62 62 ASP CB C 39.94 . 1 605 62 62 ASP N N 120.41 . 1 606 63 63 MET H H 7.48 . 1 607 63 63 MET HA H 3.83 . 1 608 63 63 MET HB2 H 1.77 . . 609 63 63 MET HG2 H 2.31 . . 610 63 63 MET HG3 H 2.70 . . 611 63 63 MET C C 177.94 . 1 612 63 63 MET CA C 58.98 . 1 613 63 63 MET CB C 32.22 . 1 614 63 63 MET N N 120.01 . 1 615 64 64 ALA H H 7.79 . 1 616 64 64 ALA HA H 4.15 . 1 617 64 64 ALA HB H 1.62 . 1 618 64 64 ALA C C 179.60 . 1 619 64 64 ALA CA C 54.71 . 1 620 64 64 ALA CB C 18.36 . 1 621 64 64 ALA N N 122.01 . 1 622 65 65 LYS H H 8.15 . 1 623 65 65 LYS HA H 4.16 . 1 624 65 65 LYS HB2 H 1.89 . . 625 65 65 LYS HB3 H 1.97 . . 626 65 65 LYS HD2 H 1.62 . . 627 65 65 LYS HD3 H 1.74 . . 628 65 65 LYS HE2 H 3.08 . 2 629 65 65 LYS HE3 H 3.08 . 2 630 65 65 LYS HG2 H 0.89 . . 631 65 65 LYS HG3 H 0.92 . . 632 65 65 LYS C C 178.81 . 1 633 65 65 LYS CA C 58.83 . 1 634 65 65 LYS CB C 32.63 . 1 635 65 65 LYS N N 119.93 . 1 636 66 66 SER H H 8.01 . 1 637 66 66 SER HA H 4.37 . 1 638 66 66 SER HB2 H 3.96 . . 639 66 66 SER HB3 H 3.99 . . 640 66 66 SER C C 175.98 . 1 641 66 66 SER CA C 60.36 . 1 642 66 66 SER CB C 63.23 . 1 643 66 66 SER N N 115.76 . 1 644 67 67 ASP H H 8.23 . 1 645 67 67 ASP HA H 4.70 . 1 646 67 67 ASP HB2 H 2.91 . . 647 67 67 ASP C C 177.19 . 1 648 67 67 ASP CA C 56.28 . 1 649 67 67 ASP CB C 42.11 . 1 650 67 67 ASP N N 122.97 . 1 651 68 68 LYS H H 8.15 . 1 652 68 68 LYS HA H 4.15 . 1 653 68 68 LYS HB2 H 1.90 . . 654 68 68 LYS HB3 H 1.98 . . 655 68 68 LYS HD2 H 1.74 . 2 656 68 68 LYS HD3 H 1.74 . 2 657 68 68 LYS HE2 H 3.03 . 2 658 68 68 LYS HE3 H 3.03 . 2 659 68 68 LYS HG2 H 1.51 . 2 660 68 68 LYS HG3 H 1.51 . 2 661 68 68 LYS C C 177.30 . 1 662 68 68 LYS CA C 58.40 . 1 663 68 68 LYS CB C 32.64 . 1 664 68 68 LYS N N 120.51 . 1 665 69 69 ALA H H 7.88 . 1 666 69 69 ALA HA H 4.27 . 1 667 69 69 ALA HB H 1.48 . 1 668 69 69 ALA C C 179.22 . 1 669 69 69 ALA CA C 54.07 . 1 670 69 69 ALA CB C 18.23 . 1 671 69 69 ALA N N 122.39 . 1 672 70 70 ARG H H 7.89 . 1 673 70 70 ARG HA H 4.11 . 1 674 70 70 ARG HB2 H 1.87 . . 675 70 70 ARG HB3 H 2.02 . . 676 70 70 ARG HD2 H 3.08 . 2 677 70 70 ARG HD3 H 3.08 . 2 678 70 70 ARG HG2 H 1.57 . . 679 70 70 ARG HG3 H 1.70 . . 680 70 70 ARG C C 177.29 . 1 681 70 70 ARG CA C 58.01 . 1 682 70 70 ARG CB C 29.65 . 1 683 70 70 ARG N N 119.39 . 1 684 71 71 TYR H H 8.23 . 1 685 71 71 TYR HA H 4.52 . 1 686 71 71 TYR HB2 H 2.97 . . 687 71 71 TYR HB3 H 3.13 . . 688 71 71 TYR HD1 H 7.22 . 3 689 71 71 TYR HD2 H 7.22 . 3 690 71 71 TYR HE1 H 6.83 . 3 691 71 71 TYR HE2 H 6.83 . 3 692 71 71 TYR C C 176.38 . 1 693 71 71 TYR CA C 60.04 . 1 694 71 71 TYR CB C 38.86 . 1 695 71 71 TYR N N 121.06 . 1 696 72 72 ASP H H 8.51 . 1 697 72 72 ASP HA H 4.44 . 1 698 72 72 ASP HB2 H 2.70 . . 699 72 72 ASP C C 177.53 . 1 700 72 72 ASP CA C 55.73 . 1 701 72 72 ASP CB C 40.48 . 1 702 72 72 ASP N N 120.12 . 1 703 73 73 ARG H H 7.97 . 1 704 73 73 ARG HA H 4.22 . 1 705 73 73 ARG HB2 H 1.91 . . 706 73 73 ARG HD2 H 3.22 . 2 707 73 73 ARG HD3 H 3.22 . 2 708 73 73 ARG HG2 H 1.67 . . 709 73 73 ARG HG3 H 1.73 . . 710 73 73 ARG C C 177.50 . 1 711 73 73 ARG CA C 57.58 . 1 712 73 73 ARG CB C 30.56 . 1 713 73 73 ARG N N 119.94 . 1 714 74 74 GLU H H 8.23 . 1 715 74 74 GLU HA H 4.19 . 1 716 74 74 GLU HB2 H 1.99 . . 717 74 74 GLU HG2 H 2.24 . . 718 74 74 GLU HG3 H 2.43 . . 719 74 74 GLU C C 177.20 . 1 720 74 74 GLU CA C 57.46 . 1 721 74 74 GLU CB C 29.94 . 1 722 74 74 GLU N N 120.10 . 1 723 75 75 MET H H 8.10 . 1 724 75 75 MET HA H 4.41 . 1 725 75 75 MET HB2 H 1.95 . . 726 75 75 MET HB3 H 2.20 . . 727 75 75 MET HG2 H 2.32 . . 728 75 75 MET HG3 H 2.42 . . 729 75 75 MET C C 176.22 . 1 730 75 75 MET CA C 55.34 . 1 731 75 75 MET CB C 32.36 . 1 732 75 75 MET N N 119.67 . 1 733 76 76 LYS H H 7.89 . 1 734 76 76 LYS HA H 4.31 . 1 735 76 76 LYS HB2 H 1.78 . . 736 76 76 LYS HB3 H 1.89 . . 737 76 76 LYS HD2 H 1.70 . 2 738 76 76 LYS HD3 H 1.70 . 2 739 76 76 LYS HE2 H 3.01 . 2 740 76 76 LYS HE3 H 3.01 . 2 741 76 76 LYS HG2 H 1.44 . . 742 76 76 LYS HG3 H 1.49 . . 743 76 76 LYS C C 175.48 . 1 744 76 76 LYS CA C 56.36 . 1 745 76 76 LYS CB C 32.85 . 1 746 76 76 LYS N N 121.91 . 1 747 77 77 ASN H H 7.98 . 1 748 77 77 ASN HA H 4.46 . 1 749 77 77 ASN HB2 H 2.65 . . 750 77 77 ASN HB3 H 2.77 . . 751 77 77 ASN HD21 H 6.81 . . 752 77 77 ASN HD22 H 7.54 . . 753 77 77 ASN CA C 54.80 . 1 754 77 77 ASN CB C 40.40 . 1 755 77 77 ASN N N 125.21 . 1 756 77 77 ASN ND2 N 112.72 . 1 stop_ save_