data_1338 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Interaction Mechanism between Microtubule-Associated Proteins and Microtubules. A Proton Nuclear Resonance Analysis on the Binding of Synthetic Peptide to Tubulin ; _BMRB_accession_number 1338 _BMRB_flat_file_name bmr1338.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kotani Susumu . . 2 Kawai Gota . . 3 Yokoyama Shigeyuki . . 4 Murofushi Hiromu . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 130 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Kotani, Susumu, Kawai, Gota, Yokoyama, Shigeyuki, Murofushi, Hiromu, "Interaction Mechanism between Microtubule-Associated Proteins and Microtubules. A Proton Nuclear Resonance Analysis on the Binding of Synthetic Peptide to Tubulin," Biochemistry 29 (43), 10049-10054 (1990). ; _Citation_title ; Interaction Mechanism between Microtubule-Associated Proteins and Microtubules. A Proton Nuclear Resonance Analysis on the Binding of Synthetic Peptide to Tubulin ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kotani Susumu . . 2 Kawai Gota . . 3 Yokoyama Shigeyuki . . 4 Murofushi Hiromu . . stop_ _Journal_abbreviation Biochemistry _Journal_volume 29 _Journal_issue 43 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 10049 _Page_last 10054 _Year 1990 _Details . save_ ################################## # Molecular system description # ################################## save_system_tubulin_binding_peptide _Saveframe_category molecular_system _Mol_system_name 'tubulin binding peptide' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'tubulin binding peptide' $tubulin_binding_peptide stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_tubulin_binding_peptide _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'tubulin binding peptide' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 23 _Mol_residue_sequence ; KNVRSKVGSTENIKHQPGGG RAK ; loop_ _Residue_seq_code _Residue_label 1 LYS 2 ASN 3 VAL 4 ARG 5 SER 6 LYS 7 VAL 8 GLY 9 SER 10 THR 11 GLU 12 ASN 13 ILE 14 LYS 15 HIS 16 GLN 17 PRO 18 GLY 19 GLY 20 GLY 21 ARG 22 ALA 23 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1339 "tubulin binding peptide" 100.00 23 100.00 100.00 7.00e-06 DBJ BAA14179 "unnamed protein product [Bos taurus]" 100.00 1072 100.00 100.00 1.41e-03 DBJ BAC07258 "microtubule-associated protein 4 isoform 1 [Bos taurus]" 100.00 696 100.00 100.00 1.26e-05 DBJ BAC07259 "microtubule-associated protein 4 isoform 2 [Bos taurus]" 91.30 710 100.00 100.00 1.07e-04 DBJ BAC07260 "microtubule-associated protein 4 isoform 3 [Bos taurus]" 100.00 135 100.00 100.00 9.07e-06 DBJ BAC56093 "microtubule-associated protein 4 isoform 5 [Bos taurus]" 100.00 483 100.00 100.00 1.58e-05 EMBL CAH18346 "hypothetical protein [Homo sapiens]" 91.30 492 100.00 100.00 1.89e-04 GB AAA59553 "microtubule-associated protein 4 [Homo sapiens]" 100.00 1152 100.00 100.00 1.30e-05 GB AAA67361 "microtubule-associated protein 4 [Homo sapiens]" 100.00 1152 100.00 100.00 1.31e-05 GB AAH08715 "MAP4 protein [Homo sapiens]" 100.00 979 100.00 100.00 1.24e-05 GB AAH12794 "MAP4 protein [Homo sapiens]" 100.00 979 100.00 100.00 1.24e-05 GB AAH15149 "MAP4 protein [Homo sapiens]" 100.00 979 100.00 100.00 1.24e-05 REF NP_001127836 "microtubule-associated protein 4 isoform 4 [Homo sapiens]" 100.00 1135 100.00 100.00 1.39e-05 REF NP_001157505 "microtubule-associated protein 4 [Felis catus]" 100.00 1135 100.00 100.00 1.26e-05 REF NP_001264869 "microtubule-associated protein 4 isoform 1 [Macaca mulatta]" 100.00 1107 100.00 100.00 1.45e-05 REF NP_002366 "microtubule-associated protein 4 isoform 1 [Homo sapiens]" 100.00 1152 100.00 100.00 1.31e-05 REF NP_776530 "microtubule-associated protein 4 [Bos taurus]" 100.00 1072 100.00 100.00 1.41e-03 SP P27816 "RecName: Full=Microtubule-associated protein 4; Short=MAP-4" 100.00 1152 100.00 100.00 1.31e-05 SP P36225 "RecName: Full=Microtubule-associated protein 4; Short=MAP-4; AltName: Full=Microtubule-associated protein U; Short=MAP-U" 100.00 1072 100.00 100.00 1.41e-03 TPG DAA16874 "TPA: microtubule-associated protein 4 [Bos taurus]" 100.00 1072 100.00 100.00 1.52e-03 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $tubulin_binding_peptide . . . . . . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $tubulin_binding_peptide 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 3.1 . na temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis DSS H . . ppm 0 . . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'tubulin binding peptide' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 LYS HA H 4.04 . 1 2 . 1 LYS HB2 H 1.9 . 1 3 . 1 LYS HB3 H 1.9 . 1 4 . 1 LYS HG2 H 1.43 . 1 5 . 1 LYS HG3 H 1.43 . 1 6 . 1 LYS HD2 H 1.7 . 1 7 . 1 LYS HD3 H 1.7 . 1 8 . 1 LYS HE2 H 3 . 1 9 . 1 LYS HE3 H 3 . 1 10 . 2 ASN H H 8.91 . 1 11 . 2 ASN HB2 H 2.76 . 2 12 . 2 ASN HB3 H 2.82 . 2 13 . 3 VAL H H 8.38 . 1 14 . 3 VAL HA H 4.16 . 1 15 . 3 VAL HB H 2.1 . 1 16 . 3 VAL HG1 H .93 . 1 17 . 3 VAL HG2 H .93 . 1 18 . 4 ARG H H 8.45 . 1 19 . 4 ARG HA H 4.36 . 1 20 . 4 ARG HB2 H 1.77 . 2 21 . 4 ARG HB3 H 1.83 . 2 22 . 4 ARG HG2 H 1.65 . 1 23 . 4 ARG HG3 H 1.65 . 1 24 . 4 ARG HD2 H 3.19 . 1 25 . 4 ARG HD3 H 3.19 . 1 26 . 5 SER H H 8.32 . 1 27 . 5 SER HA H 4.42 . 1 28 . 5 SER HB2 H 3.85 . 1 29 . 5 SER HB3 H 3.85 . 1 30 . 6 LYS H H 8.46 . 1 31 . 6 LYS HA H 4.36 . 1 32 . 6 LYS HB2 H 1.76 . 1 33 . 6 LYS HB3 H 1.76 . 1 34 . 6 LYS HG2 H 1.43 . 1 35 . 6 LYS HG3 H 1.43 . 1 36 . 6 LYS HD2 H 1.61 . 1 37 . 6 LYS HD3 H 1.61 . 1 38 . 6 LYS HE2 H 3 . 1 39 . 6 LYS HE3 H 3 . 1 40 . 7 VAL H H 8.18 . 1 41 . 7 VAL HA H 4.1 . 1 42 . 7 VAL HB H 2.06 . 1 43 . 7 VAL HG1 H .94 . 1 44 . 7 VAL HG2 H .94 . 1 45 . 8 GLY H H 8.51 . 1 46 . 8 GLY HA2 H 4 . 1 47 . 8 GLY HA3 H 4 . 1 48 . 9 SER H H 8.25 . 1 49 . 9 SER HA H 4.54 . 1 50 . 9 SER HB2 H 3.88 . 2 51 . 9 SER HB3 H 3.93 . 2 52 . 10 THR H H 8.28 . 1 53 . 10 THR HA H 4.37 . 1 54 . 10 THR HB H 4.28 . 1 55 . 10 THR HG2 H 1.2 . 1 56 . 11 GLU H H 8.31 . 1 57 . 11 GLU HA H 4.35 . 1 58 . 11 GLU HB2 H 1.97 . 2 59 . 11 GLU HB3 H 2.09 . 2 60 . 11 GLU HG2 H 2.44 . 1 61 . 11 GLU HG3 H 2.44 . 1 62 . 12 ASN H H 8.47 . 1 63 . 12 ASN HB2 H 2.74 . 2 64 . 12 ASN HB3 H 2.81 . 2 65 . 13 ILE H H 8.02 . 1 66 . 13 ILE HA H 4.11 . 1 67 . 13 ILE HB H 1.83 . 1 68 . 13 ILE HG12 H 1.16 . 2 69 . 13 ILE HG13 H 1.41 . 2 70 . 13 ILE HG2 H .8 . 1 71 . 13 ILE HD1 H .83 . 1 72 . 14 LYS H H 8.33 . 1 73 . 14 LYS HA H 4.25 . 1 74 . 14 LYS HB2 H 1.73 . 1 75 . 14 LYS HB3 H 1.73 . 1 76 . 14 LYS HG2 H 1.45 . 1 77 . 14 LYS HG3 H 1.45 . 1 78 . 14 LYS HD2 H 1.72 . 1 79 . 14 LYS HD3 H 1.72 . 1 80 . 14 LYS HE2 H 3 . 1 81 . 14 LYS HE3 H 3 . 1 82 . 15 HIS H H 8.49 . 1 83 . 15 HIS HA H 4.69 . 1 84 . 15 HIS HB2 H 3.16 . 2 85 . 15 HIS HB3 H 3.23 . 2 86 . 15 HIS HD2 H 7.29 . 1 87 . 15 HIS HE1 H 8.63 . 1 88 . 16 GLN H H 8.51 . 1 89 . 16 GLN HA H 4.62 . 1 90 . 16 GLN HB2 H 1.91 . 2 91 . 16 GLN HB3 H 2.1 . 2 92 . 16 GLN HG2 H 2.37 . 1 93 . 16 GLN HG3 H 2.37 . 1 94 . 17 PRO HA H 4.43 . 1 95 . 17 PRO HB2 H 3.67 . 2 96 . 17 PRO HB3 H 3.79 . 2 97 . 17 PRO HG2 H 1.97 . 2 98 . 17 PRO HG3 H 2.06 . 2 99 . 17 PRO HD2 H 2.3 . 1 100 . 17 PRO HD3 H 2.3 . 1 101 . 18 GLY H H 8.58 . 1 102 . 18 GLY HA2 H 4 . 1 103 . 18 GLY HA3 H 4 . 1 104 . 19 GLY H H 8.34 . 1 105 . 19 GLY HA2 H 3.99 . 1 106 . 19 GLY HA3 H 3.99 . 1 107 . 20 GLY H H 8.35 . 1 108 . 20 GLY HA2 H 3.96 . 1 109 . 20 GLY HA3 H 3.96 . 1 110 . 21 ARG H H 8.17 . 1 111 . 21 ARG HA H 4.34 . 1 112 . 21 ARG HB2 H 1.77 . 2 113 . 21 ARG HB3 H 1.83 . 2 114 . 21 ARG HG2 H 1.66 . 1 115 . 21 ARG HG3 H 1.66 . 1 116 . 21 ARG HD2 H 3.19 . 1 117 . 21 ARG HD3 H 3.19 . 1 118 . 22 ALA H H 8.37 . 1 119 . 22 ALA HA H 4.29 . 1 120 . 22 ALA HB H 1.99 . 1 121 . 23 LYS H H 8.22 . 1 122 . 23 LYS HA H 4.24 . 1 123 . 23 LYS HB2 H 1.87 . 1 124 . 23 LYS HB3 H 1.87 . 1 125 . 23 LYS HG2 H 1.43 . 1 126 . 23 LYS HG3 H 1.43 . 1 127 . 23 LYS HD2 H 1.76 . 1 128 . 23 LYS HD3 H 1.76 . 1 129 . 23 LYS HE2 H 3 . 1 130 . 23 LYS HE3 H 3 . 1 stop_ save_