data_1378 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Nuclear-magnetic-resonance studies of human epidermal growth factor ; _BMRB_accession_number 1378 _BMRB_flat_file_name bmr1378.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Cooke Robert M. . 2 Tappin Michael J. . 3 Campbell Iain D. . 4 Kohda Daisuke . . 5 Miyake Tetsuo . . 6 Fuwa Toru . . 7 Miyazawa Tatsuo . . 8 Inagaki Fuyuhiko . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 316 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-14 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Cooke, Robert M., Tappin, Michael J., Campbell, Iain D., Kohda, Daisuke, Miyake, Tetsuo, Fuwa, Toru, Miyazawa, Tatsuo, Inagaki, Fuyuhiko, "Nuclear-magnetic-resonance studies of human epidermal growth factor," Eur. J. Biochem. 193, 807-815 (1990). ; _Citation_title 'Nuclear-magnetic-resonance studies of human epidermal growth factor' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Cooke Robert M. . 2 Tappin Michael J. . 3 Campbell Iain D. . 4 Kohda Daisuke . . 5 Miyake Tetsuo . . 6 Fuwa Toru . . 7 Miyazawa Tatsuo . . 8 Inagaki Fuyuhiko . . stop_ _Journal_abbreviation 'Eur. J. Biochem.' _Journal_volume 193 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 807 _Page_last 815 _Year 1990 _Details . save_ ################################## # Molecular system description # ################################## save_system_epidermal_growth_factor _Saveframe_category molecular_system _Mol_system_name 'epidermal growth factor' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'epidermal growth factor' $epidermal_growth_factor stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_epidermal_growth_factor _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'epidermal growth factor' _Name_variant 'hEGF residues 1-53' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 53 _Mol_residue_sequence ; NSDSECPLSHDGYCLHDGVC MYIEALDKYACNCVVGYIGE RCQYRDLKWWELR ; loop_ _Residue_seq_code _Residue_label 1 ASN 2 SER 3 ASP 4 SER 5 GLU 6 CYS 7 PRO 8 LEU 9 SER 10 HIS 11 ASP 12 GLY 13 TYR 14 CYS 15 LEU 16 HIS 17 ASP 18 GLY 19 VAL 20 CYS 21 MET 22 TYR 23 ILE 24 GLU 25 ALA 26 LEU 27 ASP 28 LYS 29 TYR 30 ALA 31 CYS 32 ASN 33 CYS 34 VAL 35 VAL 36 GLY 37 TYR 38 ILE 39 GLY 40 GLU 41 ARG 42 CYS 43 GLN 44 TYR 45 ARG 46 ASP 47 LEU 48 LYS 49 TRP 50 TRP 51 GLU 52 LEU 53 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-27 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1377 "epidermal growth factor" 90.57 48 100.00 100.00 5.43e-26 BMRB 16768 hEGF 100.00 53 100.00 100.00 2.60e-30 BMRB 2075 "epidermal growth factor" 98.11 52 100.00 100.00 1.59e-29 BMRB 2076 "epidermal growth factor" 98.11 52 98.08 98.08 1.57e-28 BMRB 2077 "epidermal growth factor" 98.11 52 98.08 98.08 1.13e-28 BMRB 2078 "epidermal growth factor" 98.11 52 98.08 98.08 1.34e-28 PDB 1IVO "Crystal Structure Of The Complex Of Human Epidermal Growth Factor And Receptor Extracellular Domains." 100.00 53 100.00 100.00 2.60e-30 PDB 1JL9 "Crystal Structure Of Human Epidermal Growth Factor" 96.23 51 100.00 100.00 7.27e-29 PDB 1NQL "Structure Of The Extracellular Domain Of Human Epidermal Growth Factor (egf) Receptor In An Inactive (low Ph) Complex With Egf." 100.00 53 100.00 100.00 2.60e-30 PDB 2KV4 Egf 100.00 53 100.00 100.00 2.60e-30 PDB 3NJP "The Extracellular And Transmembrane Domain Interfaces In Epidermal Growth Factor Receptor Signaling" 88.68 47 100.00 100.00 7.14e-26 DBJ BAG61319 "unnamed protein product [Homo sapiens]" 100.00 1165 100.00 100.00 2.96e-28 EMBL CAA28240 "unnamed protein product [Homo sapiens]" 100.00 1207 100.00 100.00 3.48e-28 EMBL CCA96179 "human SP1-epidermal growth factor [synthetic construct]" 100.00 77 100.00 100.00 8.79e-31 EMBL CEK41095 "Skin permeable EGF [synthetic construct]" 100.00 99 100.00 100.00 9.68e-31 GB AAA60744 "epidermal growth factor [synthetic construct]" 100.00 54 100.00 100.00 2.12e-30 GB AAA72173 "epidermal growth factor, partial [synthetic construct]" 100.00 53 100.00 100.00 2.60e-30 GB AAA72241 "epidermal growth factor (urogastrone) [synthetic construct]" 100.00 54 100.00 100.00 2.12e-30 GB AAA72506 "epidermal growth factor, partial [synthetic construct]" 100.00 53 100.00 100.00 2.60e-30 GB AAA72563 "epidermal growth factor [synthetic construct]" 100.00 54 100.00 100.00 2.12e-30 REF NP_001171601 "pro-epidermal growth factor isoform 2 preproprotein [Homo sapiens]" 100.00 1166 100.00 100.00 2.99e-28 REF NP_001171602 "pro-epidermal growth factor isoform 3 preproprotein [Homo sapiens]" 100.00 1165 100.00 100.00 3.16e-28 REF NP_001954 "pro-epidermal growth factor isoform 1 preproprotein [Homo sapiens]" 100.00 1207 100.00 100.00 3.32e-28 REF XP_001088957 "PREDICTED: pro-epidermal growth factor isoform 1 [Macaca mulatta]" 100.00 1208 98.11 98.11 2.52e-27 REF XP_002804220 "PREDICTED: pro-epidermal growth factor isoform 2 [Macaca mulatta]" 100.00 1166 98.11 98.11 2.22e-27 SP P01133 "RecName: Full=Pro-epidermal growth factor; Short=EGF; Contains: RecName: Full=Epidermal growth factor; AltName: Full=Urogastron" 100.00 1207 100.00 100.00 3.32e-28 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $epidermal_growth_factor Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $epidermal_growth_factor 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 2.8 . na temperature 301 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'epidermal growth factor' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 ASN HA H 4.43 . 1 2 . 1 ASN HB2 H 3.02 . 1 3 . 1 ASN HB3 H 3.02 . 1 4 . 1 ASN HD21 H 7.7 . 2 5 . 1 ASN HD22 H 7.03 . 2 6 . 2 SER H H 8.8 . 1 7 . 2 SER HA H 4.58 . 1 8 . 2 SER HB2 H 3.93 . 1 9 . 2 SER HB3 H 3.93 . 1 10 . 3 ASP H H 8.53 . 1 11 . 3 ASP HA H 4.91 . 1 12 . 3 ASP HB2 H 2.92 . 1 13 . 3 ASP HB3 H 2.92 . 1 14 . 4 SER H H 8.5 . 1 15 . 4 SER HA H 4.77 . 1 16 . 4 SER HB2 H 3.96 . 2 17 . 4 SER HB3 H 4.06 . 2 18 . 5 GLU H H 8.28 . 1 19 . 5 GLU HA H 4.52 . 1 20 . 5 GLU HB2 H 2.2 . 2 21 . 5 GLU HB3 H 2.08 . 2 22 . 5 GLU HG2 H 2.58 . 1 23 . 5 GLU HG3 H 2.58 . 1 24 . 6 CYS H H 8.71 . 1 25 . 6 CYS HA H 4.44 . 1 26 . 6 CYS HB2 H 2.97 . 2 27 . 6 CYS HB3 H 3.2 . 2 28 . 7 PRO HA H 4.5 . 1 29 . 7 PRO HB2 H 1.96 . 2 30 . 7 PRO HB3 H 2.27 . 2 31 . 7 PRO HG2 H 1.83 . 2 32 . 7 PRO HG3 H 1.71 . 2 33 . 7 PRO HD2 H 3.08 . 2 34 . 7 PRO HD3 H 3.45 . 2 35 . 8 LEU H H 8.42 . 1 36 . 8 LEU HA H 4.08 . 1 37 . 8 LEU HB2 H 1.68 . 1 38 . 8 LEU HB3 H 1.68 . 1 39 . 8 LEU HG H 1.68 . 1 40 . 8 LEU HD1 H .9 . 2 41 . 8 LEU HD2 H .92 . 2 42 . 9 SER H H 8.27 . 1 43 . 9 SER HA H 4.2 . 1 44 . 9 SER HB2 H 3.97 . 1 45 . 9 SER HB3 H 3.97 . 1 46 . 10 HIS H H 8.33 . 1 47 . 10 HIS HA H 4.63 . 1 48 . 10 HIS HB2 H 3.43 . 2 49 . 10 HIS HB3 H 2.61 . 2 50 . 10 HIS HD2 H 7.35 . 1 51 . 10 HIS HE1 H 8.36 . 1 52 . 11 ASP H H 8.05 . 1 53 . 11 ASP HA H 4.6 . 1 54 . 11 ASP HB2 H 2.99 . 1 55 . 11 ASP HB3 H 2.99 . 1 56 . 12 GLY H H 8.6 . 1 57 . 12 GLY HA2 H 3.8 . 2 58 . 12 GLY HA3 H 4.01 . 2 59 . 13 TYR H H 7.65 . 1 60 . 13 TYR HA H 4.08 . 1 61 . 13 TYR HB2 H 2.88 . 2 62 . 13 TYR HB3 H 3.09 . 2 63 . 13 TYR HD1 H 7.06 . 1 64 . 13 TYR HD2 H 7.06 . 1 65 . 13 TYR HE1 H 6.77 . 1 66 . 13 TYR HE2 H 6.77 . 1 67 . 14 CYS H H 8.53 . 1 68 . 14 CYS HA H 4.36 . 1 69 . 14 CYS HB2 H 2.39 . 2 70 . 14 CYS HB3 H 2.67 . 2 71 . 15 LEU H H 8.07 . 1 72 . 15 LEU HA H 4.07 . 1 73 . 15 LEU HB2 H .76 . 2 74 . 15 LEU HB3 H 1.49 . 2 75 . 15 LEU HG H 1.55 . 1 76 . 15 LEU HD1 H .64 . 2 77 . 15 LEU HD2 H .66 . 2 78 . 16 HIS H H 8.69 . 1 79 . 16 HIS HA H 3.88 . 1 80 . 16 HIS HB2 H 2.26 . 2 81 . 16 HIS HB3 H 2.42 . 2 82 . 16 HIS HD2 H 7.42 . 1 83 . 16 HIS HE1 H 8.55 . 1 84 . 17 ASP H H 9.02 . 1 85 . 17 ASP HA H 4.32 . 1 86 . 17 ASP HB2 H 2.99 . 2 87 . 17 ASP HB3 H 3.2 . 2 88 . 18 GLY H H 7.24 . 1 89 . 18 GLY HA2 H 3.42 . 2 90 . 18 GLY HA3 H 4.33 . 2 91 . 19 VAL H H 8.2 . 1 92 . 19 VAL HA H 4.28 . 1 93 . 19 VAL HB H 2.08 . 1 94 . 19 VAL HG1 H 1.06 . 2 95 . 19 VAL HG2 H 1.08 . 2 96 . 20 CYS H H 8.9 . 1 97 . 20 CYS HA H 5.15 . 1 98 . 20 CYS HB2 H 3.34 . 1 99 . 20 CYS HB3 H 3.34 . 1 100 . 21 MET H H 9.46 . 1 101 . 21 MET HA H 4.95 . 1 102 . 21 MET HB2 H 2 . 1 103 . 21 MET HB3 H 2 . 1 104 . 21 MET HG2 H 2.48 . 1 105 . 21 MET HG3 H 2.48 . 1 106 . 21 MET HE H 2.02 . 1 107 . 22 TYR H H 8.77 . 1 108 . 22 TYR HA H 4.79 . 1 109 . 22 TYR HB2 H 2.65 . 1 110 . 22 TYR HB3 H 2.65 . 1 111 . 22 TYR HD1 H 6.21 . 1 112 . 22 TYR HD2 H 6.21 . 1 113 . 22 TYR HE1 H 6.36 . 1 114 . 22 TYR HE2 H 6.36 . 1 115 . 23 ILE H H 8.46 . 1 116 . 23 ILE HA H 4.04 . 1 117 . 23 ILE HB H 1.75 . 1 118 . 23 ILE HG12 H 1.08 . 2 119 . 23 ILE HG13 H 1.37 . 2 120 . 23 ILE HG2 H .83 . 1 121 . 23 ILE HD1 H .73 . 1 122 . 24 GLU H H 8.26 . 1 123 . 24 GLU HA H 3.33 . 1 124 . 24 GLU HB2 H 2.01 . 1 125 . 24 GLU HB3 H 2.01 . 1 126 . 24 GLU HG2 H 2.45 . 2 127 . 24 GLU HG3 H 2.56 . 2 128 . 25 ALA H H 8.82 . 1 129 . 25 ALA HA H 4.01 . 1 130 . 25 ALA HB H 1.36 . 1 131 . 26 LEU H H 6.57 . 1 132 . 26 LEU HA H 4.47 . 1 133 . 26 LEU HB2 H 1.24 . 2 134 . 26 LEU HB3 H 1.62 . 2 135 . 26 LEU HG H 1.48 . 1 136 . 26 LEU HD1 H .83 . 2 137 . 26 LEU HD2 H .87 . 2 138 . 27 ASP H H 7.8 . 1 139 . 27 ASP HA H 4.1 . 1 140 . 27 ASP HB2 H 2.75 . 2 141 . 27 ASP HB3 H 3.28 . 2 142 . 28 LYS H H 6.85 . 1 143 . 28 LYS HA H 4.69 . 1 144 . 28 LYS HB2 H 1.56 . 2 145 . 28 LYS HB3 H 1.68 . 2 146 . 28 LYS HG2 H 1.28 . 2 147 . 28 LYS HG3 H 1.36 . 2 148 . 28 LYS HD2 H 1.68 . 1 149 . 28 LYS HD3 H 1.68 . 1 150 . 28 LYS HE2 H 3.02 . 1 151 . 28 LYS HE3 H 3.02 . 1 152 . 28 LYS HZ H 7.65 . 1 153 . 29 TYR H H 8.46 . 1 154 . 29 TYR HA H 5.15 . 1 155 . 29 TYR HB2 H 2.54 . 2 156 . 29 TYR HB3 H 2.7 . 2 157 . 29 TYR HD1 H 6.99 . 1 158 . 29 TYR HD2 H 6.99 . 1 159 . 29 TYR HE1 H 6.32 . 1 160 . 29 TYR HE2 H 6.32 . 1 161 . 30 ALA H H 9.38 . 1 162 . 30 ALA HA H 4.95 . 1 163 . 30 ALA HB H 1.44 . 1 164 . 31 CYS H H 8.86 . 1 165 . 31 CYS HA H 5.41 . 1 166 . 31 CYS HB2 H 2.62 . 2 167 . 31 CYS HB3 H 2.85 . 2 168 . 32 ASN H H 9.45 . 1 169 . 32 ASN HA H 5.11 . 1 170 . 32 ASN HB2 H 2.81 . 2 171 . 32 ASN HB3 H 3.02 . 2 172 . 32 ASN HD21 H 6.83 . 2 173 . 32 ASN HD22 H 7.3 . 2 174 . 33 CYS H H 8.96 . 1 175 . 33 CYS HA H 4.75 . 1 176 . 33 CYS HB2 H 2.76 . 2 177 . 33 CYS HB3 H 3.34 . 2 178 . 34 VAL H H 8.63 . 1 179 . 34 VAL HA H 4.15 . 1 180 . 34 VAL HB H 2.21 . 1 181 . 34 VAL HG1 H 1.11 . 2 182 . 34 VAL HG2 H 1.18 . 2 183 . 35 VAL H H 7.82 . 1 184 . 35 VAL HA H 3.51 . 1 185 . 35 VAL HB H 1.65 . 1 186 . 35 VAL HG1 H .62 . 2 187 . 35 VAL HG2 H .77 . 2 188 . 36 GLY H H 8.07 . 1 189 . 36 GLY HA2 H 2.97 . 2 190 . 36 GLY HA3 H 3.9 . 2 191 . 37 TYR H H 8.17 . 1 192 . 37 TYR HA H 5.46 . 1 193 . 37 TYR HB2 H 2.86 . 2 194 . 37 TYR HB3 H 2.99 . 2 195 . 37 TYR HD1 H 6.73 . 1 196 . 37 TYR HD2 H 6.73 . 1 197 . 37 TYR HE1 H 6.3 . 1 198 . 37 TYR HE2 H 6.3 . 1 199 . 38 ILE H H 9.27 . 1 200 . 38 ILE HA H 4.88 . 1 201 . 38 ILE HB H 2.09 . 1 202 . 38 ILE HG12 H 1.02 . 2 203 . 38 ILE HG13 H 1.45 . 2 204 . 38 ILE HG2 H .93 . 1 205 . 38 ILE HD1 H .91 . 1 206 . 39 GLY H H 8.05 . 1 207 . 39 GLY HA2 H 4.01 . 2 208 . 39 GLY HA3 H 5 . 2 209 . 40 GLU H H 9.29 . 1 210 . 40 GLU HA H 4.21 . 1 211 . 40 GLU HB2 H 2.27 . 2 212 . 40 GLU HB3 H 2.31 . 2 213 . 40 GLU HG2 H 2.62 . 2 214 . 40 GLU HG3 H 2.72 . 2 215 . 41 ARG H H 8.47 . 1 216 . 41 ARG HA H 4.83 . 1 217 . 41 ARG HB2 H 1 . 2 218 . 41 ARG HB3 H 2.27 . 2 219 . 41 ARG HG2 H .7 . 2 220 . 41 ARG HG3 H 1.31 . 2 221 . 41 ARG HD2 H 2.82 . 2 222 . 41 ARG HD3 H 2.9 . 2 223 . 41 ARG HE H 7.14 . 1 224 . 42 CYS H H 7.79 . 1 225 . 42 CYS HA H 4.01 . 1 226 . 42 CYS HB2 H 3.15 . 2 227 . 42 CYS HB3 H 3.5 . 2 228 . 43 GLN H H 9.72 . 1 229 . 43 GLN HA H 4.1 . 1 230 . 43 GLN HB2 H 1.49 . 2 231 . 43 GLN HB3 H 1.8 . 2 232 . 43 GLN HG2 H 1.03 . 2 233 . 43 GLN HG3 H 1.93 . 2 234 . 43 GLN HE21 H 6.82 . 2 235 . 43 GLN HE22 H 7.42 . 2 236 . 44 TYR H H 8.74 . 1 237 . 44 TYR HA H 5.23 . 1 238 . 44 TYR HB2 H 2.72 . 2 239 . 44 TYR HB3 H 3.19 . 2 240 . 44 TYR HD1 H 7.14 . 1 241 . 44 TYR HD2 H 7.14 . 1 242 . 44 TYR HE1 H 6.95 . 1 243 . 44 TYR HE2 H 6.95 . 1 244 . 45 ARG H H 8.78 . 1 245 . 45 ARG HA H 4.33 . 1 246 . 45 ARG HB2 H 1.6 . 1 247 . 45 ARG HB3 H 1.6 . 1 248 . 45 ARG HG2 H 1.14 . 2 249 . 45 ARG HG3 H 1.5 . 2 250 . 45 ARG HD2 H 2.47 . 2 251 . 45 ARG HD3 H 2.54 . 2 252 . 45 ARG HE H 6.58 . 1 253 . 46 ASP H H 8.35 . 1 254 . 46 ASP HA H 4.64 . 1 255 . 46 ASP HB2 H 2.39 . 2 256 . 46 ASP HB3 H 2.79 . 2 257 . 47 LEU H H 8.3 . 1 258 . 47 LEU HA H 4.18 . 1 259 . 47 LEU HB2 H 1.73 . 1 260 . 47 LEU HB3 H 1.73 . 1 261 . 47 LEU HG H 1.73 . 1 262 . 47 LEU HD1 H .85 . 2 263 . 47 LEU HD2 H .95 . 2 264 . 48 LYS H H 8.04 . 1 265 . 48 LYS HA H 3.97 . 1 266 . 48 LYS HB2 H 1.61 . 2 267 . 48 LYS HB3 H 1.73 . 2 268 . 48 LYS HG2 H 1.3 . 2 269 . 48 LYS HG3 H 1.35 . 2 270 . 48 LYS HD2 H 1.65 . 1 271 . 48 LYS HD3 H 1.65 . 1 272 . 48 LYS HE2 H 2.96 . 1 273 . 48 LYS HE3 H 2.96 . 1 274 . 48 LYS HZ H 7.53 . 1 275 . 49 TRP H H 7.47 . 1 276 . 49 TRP HA H 4.14 . 1 277 . 49 TRP HB2 H 3.13 . 2 278 . 49 TRP HB3 H 3.17 . 2 279 . 49 TRP HD1 H 6.85 . 1 280 . 49 TRP HE1 H 9.94 . 1 281 . 49 TRP HE3 H 6.65 . 1 282 . 49 TRP HZ2 H 7.3 . 1 283 . 49 TRP HZ3 H 6.13 . 1 284 . 49 TRP HH2 H 6.96 . 1 285 . 50 TRP H H 7.43 . 1 286 . 50 TRP HA H 4.11 . 1 287 . 50 TRP HB2 H 3.1 . 2 288 . 50 TRP HB3 H 3.14 . 2 289 . 50 TRP HD1 H 7.35 . 1 290 . 50 TRP HE1 H 9.95 . 1 291 . 50 TRP HE3 H 7.44 . 1 292 . 50 TRP HZ2 H 7.35 . 1 293 . 50 TRP HZ3 H 7.2 . 1 294 . 50 TRP HH2 H 7.32 . 1 295 . 51 GLU H H 7.74 . 1 296 . 51 GLU HA H 4.23 . 1 297 . 51 GLU HB2 H 1.98 . 2 298 . 51 GLU HB3 H 2.07 . 2 299 . 51 GLU HG2 H 2.35 . 1 300 . 51 GLU HG3 H 2.35 . 1 301 . 52 LEU H H 7.52 . 1 302 . 52 LEU HA H 4.28 . 1 303 . 52 LEU HB2 H 1.57 . 1 304 . 52 LEU HB3 H 1.57 . 1 305 . 52 LEU HG H 1.65 . 1 306 . 52 LEU HD1 H .83 . 2 307 . 52 LEU HD2 H .87 . 2 308 . 53 ARG H H 7.72 . 1 309 . 53 ARG HA H 4.17 . 1 310 . 53 ARG HB2 H 1.63 . 2 311 . 53 ARG HB3 H 1.81 . 2 312 . 53 ARG HG2 H 1.41 . 1 313 . 53 ARG HG3 H 1.41 . 1 314 . 53 ARG HD2 H 2.7 . 2 315 . 53 ARG HD3 H 2.78 . 2 316 . 53 ARG HE H 6.84 . 1 stop_ save_