data_142 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR Study of the Solution Conformation of Rat Atrial Natriuretic Factor 7-23 in Sodium Dodecyl Sulfate Micelles ; _BMRB_accession_number 142 _BMRB_flat_file_name bmr142.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Olejniczak E. T. . 2 Gampe Robert T. . 3 Rockway T. W. . 4 Fesik Stephen W. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 82 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-10 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Olejniczak, E.T., Gampe, Robert T., Jr., Rockway, T. W., Fesik, Stephen W., "NMR Study of the Solution Conformation of Rat Atrial Natriuretic Factor 7-23 in Sodium Dodecyl Sulfate Micelles," Biochemistry 27 (18), 7124-7131 (1988). ; _Citation_title ; NMR Study of the Solution Conformation of Rat Atrial Natriuretic Factor 7-23 in Sodium Dodecyl Sulfate Micelles ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Olejniczak E. T. . 2 Gampe Robert T. . 3 Rockway T. W. . 4 Fesik Stephen W. . stop_ _Journal_abbreviation Biochemistry _Journal_volume 27 _Journal_issue 18 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 7124 _Page_last 7131 _Year 1988 _Details . save_ ################################## # Molecular system description # ################################## save_system_atrial_natriuretic_factor _Saveframe_category molecular_system _Mol_system_name 'atrial natriuretic factor' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'atrial natriuretic factor' $atrial_natriuretic_factor stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_atrial_natriuretic_factor _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'atrial natriuretic factor' _Name_variant 'residues 7-23' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 23 _Mol_residue_sequence ; XXXXXXCFGGRIDRIGAQSG LGC ; loop_ _Residue_seq_code _Residue_label 1 X 2 X 3 X 4 X 5 X 6 X 7 CYS 8 PHE 9 GLY 10 GLY 11 ARG 12 ILE 13 ASP 14 ARG 15 ILE 16 GLY 17 ALA 18 GLN 19 SER 20 GLY 21 LEU 22 GLY 23 CYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-21 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1T34 "Rotation Mechanism For Transmembrane Signaling By The Atrial Natriuretic Peptide Receptor" 73.91 21 100.00 100.00 1.58e-01 DBJ BAE27742 "unnamed protein product [Mus musculus]" 73.91 152 100.00 100.00 1.47e-03 EMBL CAA25285 "unnamed protein product [Rattus norvegicus]" 73.91 152 100.00 100.00 7.22e-03 EMBL CAA25586 "unnamed protein product [Rattus norvegicus]" 73.91 152 100.00 100.00 7.22e-03 GB AAA31162 "atrial natriuretic factor precursor [Oryctolagus cuniculus]" 73.91 153 100.00 100.00 1.00e-03 GB AAA37235 "prepronatriodilatin, partial [Mus musculus]" 73.91 152 100.00 100.00 1.04e-03 GB AAA40735 "prepronatriodilatin [Rattus norvegicus]" 73.91 152 100.00 100.00 7.22e-03 GB AAA40736 "atrial natriuretic factor, partial [Rattus norvegicus]" 73.91 115 100.00 100.00 1.02e-02 GB AAA40737 "atrial natriuretic peptide [Rattus norvegicus]" 73.91 152 100.00 100.00 8.73e-03 PRF 1001137A "atria peptide" 73.91 28 100.00 100.00 1.00e-01 PRF 1003196A "atrial natriuretic factor H1" 73.91 73 100.00 100.00 5.16e-02 PRF 1004222A "atrial natriuretic factor" 73.91 25 100.00 100.00 1.15e-01 PRF 1004225A "atrial natriuretic factor beta" 73.91 48 100.00 100.00 5.41e-02 PRF 1005179A "atriopeptin I" 73.91 21 100.00 100.00 1.13e-01 REF NP_001075731 "natriuretic peptides A precursor [Oryctolagus cuniculus]" 73.91 153 100.00 100.00 1.00e-03 REF NP_032751 "natriuretic peptides A preproprotein [Mus musculus]" 73.91 152 100.00 100.00 1.47e-03 REF NP_036744 "natriuretic peptides A precursor [Rattus norvegicus]" 73.91 152 100.00 100.00 7.22e-03 REF XP_001371445 "PREDICTED: natriuretic peptides A [Monodelphis domestica]" 73.91 144 100.00 100.00 1.96e-03 REF XP_003765045 "PREDICTED: natriuretic peptides A [Sarcophilus harrisii]" 73.91 217 100.00 100.00 2.84e-02 SP P01161 "RecName: Full=Natriuretic peptides A; AltName: Full=Prepronatriodilatin; Contains: RecName: Full=Atrial natriuretic factor; Sho" 73.91 152 100.00 100.00 7.22e-03 SP P05125 "RecName: Full=Natriuretic peptides A; AltName: Full=Prepronatriodilatin; Contains: RecName: Full=Atrial natriuretic factor; Sho" 73.91 152 100.00 100.00 1.47e-03 SP P07500 "RecName: Full=Natriuretic peptides A; AltName: Full=Prepronatriodilatin; Contains: RecName: Full=Atrial natriuretic factor; Sho" 73.91 153 100.00 100.00 1.00e-03 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $atrial_natriuretic_factor rabbit 9986 Eukaryota Metazoa Oryctolagus cuniculus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $atrial_natriuretic_factor 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5 . na temperature 313 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label TSP H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'atrial natriuretic factor' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 7 CYS HA H 4.35 . 1 2 . 7 CYS HB2 H 3.26 . 1 3 . 7 CYS HB3 H 3.26 . 1 4 . 8 PHE H H 8.83 . 1 5 . 8 PHE HA H 4.67 . 1 6 . 8 PHE HB2 H 3.11 . 1 7 . 8 PHE HB3 H 3.11 . 1 8 . 8 PHE HD1 H 7.26 . 1 9 . 8 PHE HD2 H 7.26 . 1 10 . 8 PHE HE1 H 7.26 . 1 11 . 8 PHE HE2 H 7.26 . 1 12 . 8 PHE HZ H 7.16 . 1 13 . 9 GLY H H 8.41 . 1 14 . 9 GLY HA2 H 3.67 . 2 15 . 9 GLY HA3 H 3.97 . 2 16 . 10 GLY H H 8.08 . 1 17 . 10 GLY HA2 H 3.87 . 2 18 . 10 GLY HA3 H 3.91 . 2 19 . 11 ARG H H 8.07 . 1 20 . 11 ARG HA H 4.22 . 1 21 . 11 ARG HB2 H 1.85 . 1 22 . 11 ARG HB3 H 1.85 . 1 23 . 11 ARG HG2 H 1.71 . 1 24 . 11 ARG HG3 H 1.64 . 1 25 . 11 ARG HD2 H 3.16 . 1 26 . 11 ARG HD3 H 3.16 . 1 27 . 12 ILE H H 7.56 . 1 28 . 12 ILE HA H 4.18 . 1 29 . 12 ILE HB H 1.98 . 1 30 . 12 ILE HG12 H 1.16 . 2 31 . 12 ILE HG13 H 1.48 . 2 32 . 12 ILE HG2 H .82 . 1 33 . 12 ILE HD1 H .92 . 1 34 . 13 ASP H H 8.22 . 1 35 . 13 ASP HA H 4.5 . 1 36 . 13 ASP HB2 H 2.81 . 1 37 . 13 ASP HB3 H 2.81 . 1 38 . 14 ARG H H 7.86 . 1 39 . 14 ARG HA H 4.2 . 1 40 . 14 ARG HB2 H 1.83 . 2 41 . 14 ARG HB3 H 1.87 . 2 42 . 14 ARG HG2 H 1.68 . 1 43 . 14 ARG HG3 H 1.68 . 1 44 . 14 ARG HD2 H 3.19 . 1 45 . 14 ARG HD3 H 3.19 . 1 46 . 15 ILE H H 7.48 . 1 47 . 15 ILE HA H 4.14 . 1 48 . 15 ILE HB H 1.96 . 1 49 . 15 ILE HG12 H 1.16 . 2 50 . 15 ILE HG13 H 1.51 . 2 51 . 15 ILE HG2 H .87 . 1 52 . 15 ILE HD1 H .92 . 1 53 . 16 GLY H H 8.11 . 1 54 . 16 GLY HA2 H 3.9 . 2 55 . 16 GLY HA3 H 3.95 . 2 56 . 17 ALA H H 7.91 . 1 57 . 17 ALA HA H 4.25 . 1 58 . 17 ALA HB H 1.41 . 1 59 . 18 GLN H H 8.16 . 1 60 . 18 GLN HA H 4.31 . 1 61 . 18 GLN HB2 H 2.05 . 2 62 . 18 GLN HB3 H 2.19 . 2 63 . 19 SER H H 7.98 . 1 64 . 19 SER HA H 4.4 . 1 65 . 19 SER HB2 H 3.89 . 1 66 . 19 SER HB3 H 3.89 . 1 67 . 20 GLY H H 8.16 . 1 68 . 20 GLY HA2 H 3.91 . 2 69 . 20 GLY HA3 H 3.99 . 2 70 . 21 LEU H H 7.92 . 1 71 . 21 LEU HA H 4.36 . 1 72 . 21 LEU HB2 H 1.65 . 1 73 . 21 LEU HB3 H 1.65 . 1 74 . 21 LEU HG H 1.86 . 1 75 . 21 LEU HD1 H .9 . 1 76 . 21 LEU HD2 H .9 . 1 77 . 22 GLY H H 8.24 . 1 78 . 22 GLY HA2 H 3.94 . 1 79 . 23 CYS H H 7.84 . 1 80 . 23 CYS HA H 4.44 . 1 81 . 23 CYS HB2 H 3.04 . 1 82 . 23 CYS HB3 H 3.04 . 1 stop_ save_