data_1590 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; A protein structural change in aglycosylated IgG3 correlates with loss of huFcgammaR1 and huFcgammaR111 binding and/or activation ; _BMRB_accession_number 1590 _BMRB_flat_file_name bmr1590.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-04-13 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lund John . . 2 Tanaka Toshiyuki . . 3 Takahashi Noriko . . 4 Sarmay Gabriella . . 5 Arata Yoji . . 6 Jefferis Royston . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 2 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1996-04-13 revision BMRB 'Link to the Protein Data Bank added' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Lund, John, Tanaka, Toshiyuki, Takahashi, Noriko, Sarmay, Gabriella, Arata, Yoji, Jefferis, Royston, "A protein structural change in aglycosylated IgG3 correlates with loss of huFcgammaR1 and huFcgammaR111 binding and/or activation," Mol. Immunol. 27 (11), 1145-1153 (1990). ; _Citation_title ; A protein structural change in aglycosylated IgG3 correlates with loss of huFcgammaR1 and huFcgammaR111 binding and/or activation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lund John . . 2 Tanaka Toshiyuki . . 3 Takahashi Noriko . . 4 Sarmay Gabriella . . 5 Arata Yoji . . 6 Jefferis Royston . . stop_ _Journal_abbreviation 'Mol. Immunol.' _Journal_volume 27 _Journal_issue 11 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1145 _Page_last 1153 _Year 1990 _Details . save_ ################################## # Molecular system description # ################################## save_system_anti-NIP_IgG3_immunoglobulin _Saveframe_category molecular_system _Mol_system_name 'anti-NIP IgG3 immunoglobulin' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'anti-NIP IgG3 immunoglobulin' $anti-NIP_IgG3_immunoglobulin stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_anti-NIP_IgG3_immunoglobulin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'anti-NIP IgG3 immunoglobulin' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 433 _Mol_residue_sequence ; XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXHXXXXXXXXXXXX XXXXHXXXXXXXXXXXXXXX XXXXXXXXXHXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXH ; loop_ _Residue_seq_code _Residue_label 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 X 10 X 11 X 12 X 13 X 14 X 15 X 16 X 17 X 18 X 19 X 20 X 21 X 22 X 23 X 24 X 25 X 26 X 27 X 28 X 29 X 30 X 31 X 32 X 33 X 34 X 35 X 36 X 37 X 38 X 39 X 40 X 41 X 42 X 43 X 44 X 45 X 46 X 47 X 48 X 49 X 50 X 51 X 52 X 53 X 54 X 55 X 56 X 57 X 58 X 59 X 60 X 61 X 62 X 63 X 64 X 65 X 66 X 67 X 68 X 69 X 70 X 71 X 72 X 73 X 74 X 75 X 76 X 77 X 78 X 79 X 80 X 81 X 82 X 83 X 84 X 85 X 86 X 87 X 88 X 89 X 90 X 91 X 92 X 93 X 94 X 95 X 96 X 97 X 98 X 99 X 100 X 101 X 102 X 103 X 104 X 105 X 106 X 107 X 108 X 109 X 110 X 111 X 112 X 113 X 114 X 115 X 116 X 117 X 118 X 119 X 120 X 121 X 122 X 123 X 124 X 125 X 126 X 127 X 128 X 129 X 130 X 131 X 132 X 133 X 134 X 135 X 136 X 137 X 138 X 139 X 140 X 141 X 142 X 143 X 144 X 145 X 146 X 147 X 148 X 149 X 150 X 151 X 152 X 153 X 154 X 155 X 156 X 157 X 158 X 159 X 160 X 161 X 162 X 163 X 164 X 165 X 166 X 167 X 168 X 169 X 170 X 171 X 172 X 173 X 174 X 175 X 176 X 177 X 178 X 179 X 180 X 181 X 182 X 183 X 184 X 185 X 186 X 187 X 188 X 189 X 190 X 191 X 192 X 193 X 194 X 195 X 196 X 197 X 198 X 199 X 200 X 201 X 202 X 203 X 204 X 205 X 206 X 207 X 208 X 209 X 210 X 211 X 212 X 213 X 214 X 215 X 216 X 217 X 218 X 219 X 220 X 221 X 222 X 223 X 224 X 225 X 226 X 227 X 228 X 229 X 230 X 231 X 232 X 233 X 234 X 235 X 236 X 237 X 238 X 239 X 240 X 241 X 242 X 243 X 244 X 245 X 246 X 247 X 248 X 249 X 250 X 251 X 252 X 253 X 254 X 255 X 256 X 257 X 258 X 259 X 260 X 261 X 262 X 263 X 264 X 265 X 266 X 267 X 268 HIS 269 X 270 X 271 X 272 X 273 X 274 X 275 X 276 X 277 X 278 X 279 X 280 X 281 X 282 X 283 X 284 X 285 HIS 286 X 287 X 288 X 289 X 290 X 291 X 292 X 293 X 294 X 295 X 296 X 297 X 298 X 299 X 300 X 301 X 302 X 303 X 304 X 305 X 306 X 307 X 308 X 309 X 310 HIS 311 X 312 X 313 X 314 X 315 X 316 X 317 X 318 X 319 X 320 X 321 X 322 X 323 X 324 X 325 X 326 X 327 X 328 X 329 X 330 X 331 X 332 X 333 X 334 X 335 X 336 X 337 X 338 X 339 X 340 X 341 X 342 X 343 X 344 X 345 X 346 X 347 X 348 X 349 X 350 X 351 X 352 X 353 X 354 X 355 X 356 X 357 X 358 X 359 X 360 X 361 X 362 X 363 X 364 X 365 X 366 X 367 X 368 X 369 X 370 X 371 X 372 X 373 X 374 X 375 X 376 X 377 X 378 X 379 X 380 X 381 X 382 X 383 X 384 X 385 X 386 X 387 X 388 X 389 X 390 X 391 X 392 X 393 X 394 X 395 X 396 X 397 X 398 X 399 X 400 X 401 X 402 X 403 X 404 X 405 X 406 X 407 X 408 X 409 X 410 X 411 X 412 X 413 X 414 X 415 X 416 X 417 X 418 X 419 X 420 X 421 X 422 X 423 X 424 X 425 X 426 X 427 X 428 X 429 X 430 X 431 X 432 X 433 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $anti-NIP_IgG3_immunoglobulin 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.3 . na temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis DSS H . . ppm 0 . . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'anti-NIP IgG3 immunoglobulin' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 268 HIS HD2 H 6.7 . 1 2 . 268 HIS HE1 H 7.7 . 1 stop_ save_