data_1727 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Nuclear-magnetic-resonance studies on the conformation of membrane-bound alpha-mating factor (Transferred nuclear Overhauser effect analysis) ; _BMRB_accession_number 1727 _BMRB_flat_file_name bmr1727.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Wakamatsu Kaori . . 2 Okada Akihiko . . 3 Suzuki Midoriko . . 4 Higashijima Tsutomu . . 5 Masui Yoshihiro . . 6 Sakakibara Shumpei . . 7 Miyazawa Tatsuo . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 81 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Wakamatsu, Kaori, Okada, Akihiko, Suzuki, Midoriko, Higashijima, Tsutomu, Masui, Yoshihiro, Sakakibara, Shumpei, Miyazawa, Tatsuo, "Nuclear-magnetic-resonance studies on the conformation of membrane-bound alpha-mating factor (Transferred nuclear Overhauser effect analysis)," Eur. J. Biochem. 154, 607-615 (1986). ; _Citation_title ; Nuclear-magnetic-resonance studies on the conformation of membrane-bound alpha-mating factor (Transferred nuclear Overhauser effect analysis) ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Wakamatsu Kaori . . 2 Okada Akihiko . . 3 Suzuki Midoriko . . 4 Higashijima Tsutomu . . 5 Masui Yoshihiro . . 6 Sakakibara Shumpei . . 7 Miyazawa Tatsuo . . stop_ _Journal_abbreviation 'Eur. J. Biochem.' _Journal_volume 154 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 607 _Page_last 615 _Year 1986 _Details . save_ ################################## # Molecular system description # ################################## save_system_alpha-mating_factor _Saveframe_category molecular_system _Mol_system_name 'alpha-mating factor' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'alpha-mating factor' $alpha-mating_factor stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_alpha-mating_factor _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'alpha-mating factor' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 13 _Mol_residue_sequence WHWLQLKPGQPMY loop_ _Residue_seq_code _Residue_label 1 TRP 2 HIS 3 TRP 4 LEU 5 GLN 6 LEU 7 LYS 8 PRO 9 GLY 10 GLN 11 PRO 12 MET 13 TYR stop_ _Sequence_homology_query_date 2005-09-22 _Sequence_homology_query_revised_last_date 2005-09-01 save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $alpha-mating_factor chicken . . . Gallus gallus generic stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $alpha-mating_factor 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . na temperature 323 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis DSS H . . ppm 0 . . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'alpha-mating factor' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 TRP HA H 4.09 . 1 2 . 1 TRP HB2 H 3.1 . 1 3 . 1 TRP HB3 H 3.1 . 1 4 . 1 TRP HD1 H 7.1 . 1 5 . 1 TRP HE3 H 7.47 . 1 6 . 1 TRP HZ2 H 7.41 . 1 7 . 1 TRP HZ3 H 7.06 . 1 8 . 1 TRP HH2 H 7.19 . 1 9 . 2 HIS HA H 4.49 . 1 10 . 2 HIS HB2 H 2.92 . 1 11 . 2 HIS HB3 H 2.92 . 1 12 . 2 HIS HD2 H 6.88 . 1 13 . 2 HIS HE1 H 7.75 . 1 14 . 3 TRP HA H 4.5 . 1 15 . 3 TRP HB2 H 3.18 . 1 16 . 3 TRP HB3 H 3.18 . 1 17 . 3 TRP HD1 H 7.15 . 1 18 . 3 TRP HE3 H 7.5 . 1 19 . 3 TRP HZ2 H 7.44 . 1 20 . 3 TRP HZ3 H 7.12 . 1 21 . 3 TRP HH2 H 7.19 . 1 22 . 4 LEU HA H 4.22 . 1 23 . 4 LEU HB2 H 1.46 . 1 24 . 4 LEU HB3 H 1.46 . 1 25 . 4 LEU HG H 1.32 . 1 26 . 4 LEU HD1 H .77 . 2 27 . 4 LEU HD2 H .82 . 2 28 . 5 GLN HA H 4.25 . 1 29 . 5 GLN HB2 H 1.92 . 2 30 . 5 GLN HB3 H 2.04 . 2 31 . 5 GLN HG2 H 2.25 . 1 32 . 5 GLN HG3 H 2.25 . 1 33 . 6 LEU HA H 4.32 . 1 34 . 6 LEU HB2 H 1.56 . 1 35 . 6 LEU HB3 H 1.56 . 1 36 . 6 LEU HG H 1.56 . 1 37 . 6 LEU HD1 H .82 . 2 38 . 6 LEU HD2 H .87 . 2 39 . 7 LYS HA H 4.59 . 1 40 . 7 LYS HB2 H 1.65 . 2 41 . 7 LYS HB3 H 1.77 . 2 42 . 7 LYS HG2 H 1.4 . 1 43 . 7 LYS HG3 H 1.4 . 1 44 . 7 LYS HD2 H 1.65 . 1 45 . 7 LYS HD3 H 1.65 . 1 46 . 7 LYS HE2 H 2.96 . 1 47 . 7 LYS HE3 H 2.96 . 1 48 . 8 PRO HA H 4.32 . 1 49 . 8 PRO HB2 H 1.91 . 2 50 . 8 PRO HB3 H 2.28 . 2 51 . 8 PRO HG2 H 2 . 1 52 . 8 PRO HG3 H 2 . 1 53 . 8 PRO HD2 H 3.75 . 2 54 . 8 PRO HD3 H 3.59 . 2 55 . 9 GLY HA2 H 4.01 . 2 56 . 9 GLY HA3 H 3.97 . 2 57 . 10 GLN HA H 4.64 . 1 58 . 10 GLN HB2 H 1.96 . 2 59 . 10 GLN HB3 H 2.08 . 2 60 . 10 GLN HG2 H 2.33 . 1 61 . 10 GLN HG3 H 2.33 . 1 62 . 11 PRO HA H 4.38 . 1 63 . 11 PRO HB2 H 1.77 . 2 64 . 11 PRO HB3 H 2.17 . 2 65 . 11 PRO HG2 H 2 . 1 66 . 11 PRO HG3 H 2 . 1 67 . 11 PRO HD2 H 3.7 . 2 68 . 11 PRO HD3 H 3.6 . 2 69 . 12 MET HA H 4.35 . 1 70 . 12 MET HB2 H 1.84 . 2 71 . 12 MET HB3 H 1.91 . 2 72 . 12 MET HG2 H 2.4 . 2 73 . 12 MET HG3 H 2.46 . 2 74 . 12 MET HE H 2.02 . 1 75 . 13 TYR HA H 4.37 . 1 76 . 13 TYR HB2 H 3.08 . 2 77 . 13 TYR HB3 H 2.85 . 2 78 . 13 TYR HD1 H 7.06 . 1 79 . 13 TYR HD2 H 7.06 . 1 80 . 13 TYR HE1 H 6.78 . 1 81 . 13 TYR HE2 H 6.78 . 1 stop_ save_