data_5626 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein ; _BMRB_accession_number 5626 _BMRB_flat_file_name bmr5626.str _Entry_type original _Submission_date 2002-12-18 _Accession_date 2002-12-18 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Shimizu Masato . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 145 "13C chemical shifts" 318 "15N chemical shifts" 145 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2003-06-10 original author . stop_ _Original_release_date 2003-06-10 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Letter to the editor: 1H 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Shimizu Masato . . 2 Hiroaki Hidekazu . . 3 Kohda Daisuke . . 4 Morita Eugene Hayato . 5 Hotta Shigeki . . 6 Morikawa Kosuke . . stop_ _Journal_abbreviation 'J. Biomol. NMR' _Journal_volume 26 _Journal_issue 3 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 277 _Page_last 278 _Year 2003 _Details . loop_ _Keyword 'DNA binding domain' 'glial cell missing' gliogenesis stop_ save_ ################################## # Molecular system description # ################################## save_system_GCM _Saveframe_category molecular_system _Mol_system_name 'Droshophila GCM' _Abbreviation_common GCM _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'GCM monomer' $GCM_monomer stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not reported' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_GCM_monomer _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'glial cell missing' _Name_variant AS157 _Abbreviation_common GCM _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 159 _Mol_residue_sequence ; GPATKSRVAIDWDINDSKMP SVGEFDDFNDWSNGHCRLIY SVQSDEARKHASGWAMRNTN NHNVNILKKSCLGVLLCSAK CKLPNGASVHLRPAICDKAR RKQQGKQCPNRNCNGRLEIQ ACRGHCGYPVTHFWRRDGNG IYFQAKGTHDHPRPEAKGS ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 PRO 3 ALA 4 THR 5 LYS 6 SER 7 ARG 8 VAL 9 ALA 10 ILE 11 ASP 12 TRP 13 ASP 14 ILE 15 ASN 16 ASP 17 SER 18 LYS 19 MET 20 PRO 21 SER 22 VAL 23 GLY 24 GLU 25 PHE 26 ASP 27 ASP 28 PHE 29 ASN 30 ASP 31 TRP 32 SER 33 ASN 34 GLY 35 HIS 36 CYS 37 ARG 38 LEU 39 ILE 40 TYR 41 SER 42 VAL 43 GLN 44 SER 45 ASP 46 GLU 47 ALA 48 ARG 49 LYS 50 HIS 51 ALA 52 SER 53 GLY 54 TRP 55 ALA 56 MET 57 ARG 58 ASN 59 THR 60 ASN 61 ASN 62 HIS 63 ASN 64 VAL 65 ASN 66 ILE 67 LEU 68 LYS 69 LYS 70 SER 71 CYS 72 LEU 73 GLY 74 VAL 75 LEU 76 LEU 77 CYS 78 SER 79 ALA 80 LYS 81 CYS 82 LYS 83 LEU 84 PRO 85 ASN 86 GLY 87 ALA 88 SER 89 VAL 90 HIS 91 LEU 92 ARG 93 PRO 94 ALA 95 ILE 96 CYS 97 ASP 98 LYS 99 ALA 100 ARG 101 ARG 102 LYS 103 GLN 104 GLN 105 GLY 106 LYS 107 GLN 108 CYS 109 PRO 110 ASN 111 ARG 112 ASN 113 CYS 114 ASN 115 GLY 116 ARG 117 LEU 118 GLU 119 ILE 120 GLN 121 ALA 122 CYS 123 ARG 124 GLY 125 HIS 126 CYS 127 GLY 128 TYR 129 PRO 130 VAL 131 THR 132 HIS 133 PHE 134 TRP 135 ARG 136 ARG 137 ASP 138 GLY 139 ASN 140 GLY 141 ILE 142 TYR 143 PHE 144 GLN 145 ALA 146 LYS 147 GLY 148 THR 149 HIS 150 ASP 151 HIS 152 PRO 153 ARG 154 PRO 155 GLU 156 ALA 157 LYS 158 GLY 159 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAA10905 "gcm protein [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.16e-114 GB AAC46912 "GCM [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.16e-114 GB AAC47808 "glide/gcm [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.16e-114 GB AAF52790 "glial cells missing, isoform A [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.00e-114 GB ABK30923 "RT01048p [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.00e-114 GB AGB92827 "glial cells missing, isoform B [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.00e-114 REF NP_001260292 "glial cells missing, isoform B [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.00e-114 REF NP_477108 "glial cells missing, isoform A [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.00e-114 REF XP_001969320 "GG24019 [Drosophila erecta]" 99.37 710 98.10 99.37 3.57e-110 REF XP_002036350 "GM12386 [Drosophila sechellia]" 99.37 506 99.37 100.00 1.43e-113 REF XP_002088946 "GE10457 [Drosophila yakuba]" 99.37 511 98.10 99.37 1.54e-112 SP Q27403 "RecName: Full=Transcription factor glial cells missing; AltName: Full=Protein glide [Drosophila melanogaster]" 99.37 504 100.00 100.00 5.00e-114 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Tissue _Gene_mnemonic $GCM_monomer 'fruit fly' 7227 Eukaryota Metazoa Drosophila melanogaster glia gcm stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $GCM_monomer 'recombinant technology' . . . . . 'pGEMEX derived thioredoxin fusion protein expressed in E.coli.' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $GCM_monomer 0.6 mM '[U-99% 13C; U-99% 15N]' 'potasium phosptae buffer' 20 mM . KCl 0.3 M . Glycerol 10 % . stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version 'release 2002' _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 600 _Details . save_ save_NMR_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 750 _Details . save_ ############################# # NMR applied experiments # ############################# save_1H-15N_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1H-15N NOESY' _Sample_label . save_ save_1H-15N_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '1H-15N TOCSY' _Sample_label . save_ save_HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name HNCA _Sample_label . save_ save_HN(CO)CA_4 _Saveframe_category NMR_applied_experiment _Experiment_name HN(CO)CA _Sample_label . save_ save_CBCACONH_5 _Saveframe_category NMR_applied_experiment _Experiment_name CBCACONH _Sample_label . save_ save_HNCACB_6 _Saveframe_category NMR_applied_experiment _Experiment_name HNCACB _Sample_label . save_ save_HN(CA)CO_7 _Saveframe_category NMR_applied_experiment _Experiment_name HN(CA)CO _Sample_label . save_ save_HNCO_8 _Saveframe_category NMR_applied_experiment _Experiment_name HNCO _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1H-15N NOESY' _BMRB_pulse_sequence_accession_number . _Details 'TXI probes.' save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '1H-15N TOCSY' _BMRB_pulse_sequence_accession_number . _Details 'TXI probes.' save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name HNCA _BMRB_pulse_sequence_accession_number . _Details 'TXI probes.' save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name HN(CO)CA _BMRB_pulse_sequence_accession_number . _Details 'TXI probes.' save_ save_NMR_spec_expt__0_5 _Saveframe_category NMR_applied_experiment _Experiment_name CBCACONH _BMRB_pulse_sequence_accession_number . _Details 'TXI probes.' save_ save_NMR_spec_expt__0_6 _Saveframe_category NMR_applied_experiment _Experiment_name HNCACB _BMRB_pulse_sequence_accession_number . _Details 'TXI probes.' save_ save_NMR_spec_expt__0_7 _Saveframe_category NMR_applied_experiment _Experiment_name HN(CA)CO _BMRB_pulse_sequence_accession_number . _Details 'TXI probes.' save_ save_NMR_spec_expt__0_8 _Saveframe_category NMR_applied_experiment _Experiment_name HNCO _BMRB_pulse_sequence_accession_number . _Details 'TXI probes.' save_ ####################### # Sample conditions # ####################### save_condition_number_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.66 0.03 M pH* 6.5 0.1 n/a temperature 293 1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $condition_number_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'GCM monomer' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 GLY C C 2.200 0.10 1 2 . 1 GLY CA C 2.200 0.10 1 3 . 2 PRO C C 176.393 0.10 1 4 . 2 PRO CA C 62.772 0.10 1 5 . 3 ALA H H 8.365 0.02 1 6 . 3 ALA C C 177.691 0.10 1 7 . 3 ALA CA C 51.919 0.10 1 8 . 3 ALA N N 124.084 0.20 1 9 . 4 THR H H 8.196 0.02 1 10 . 4 THR C C 173.619 0.10 1 11 . 4 THR CA C 63.207 0.10 1 12 . 4 THR N N 116.427 0.20 1 13 . 5 LYS H H 8.344 0.02 1 14 . 5 LYS C C 175.645 0.10 1 15 . 5 LYS CA C 55.707 0.10 1 16 . 5 LYS N N 125.041 0.20 1 17 . 6 SER H H 8.260 0.02 1 18 . 6 SER C C 175.417 0.10 1 19 . 6 SER CA C 56.540 0.10 1 20 . 6 SER N N 116.017 0.20 1 21 . 7 ARG H H 9.686 0.02 1 22 . 7 ARG C C 176.127 0.10 1 23 . 7 ARG CA C 56.407 0.10 1 24 . 7 ARG N N 126.134 0.20 1 25 . 8 VAL H H 8.562 0.02 1 26 . 8 VAL C C 175.494 0.10 1 27 . 8 VAL CA C 62.178 0.10 1 28 . 8 VAL N N 122.306 0.20 1 29 . 9 ALA H H 8.449 0.02 1 30 . 9 ALA C C 176.648 0.10 1 31 . 9 ALA CA C 51.919 0.10 1 32 . 9 ALA N N 129.963 0.20 1 33 . 10 ILE H H 7.558 0.02 1 34 . 10 ILE C C 174.911 0.10 1 35 . 10 ILE CA C 60.214 0.10 1 36 . 10 ILE N N 119.162 0.20 1 37 . 11 ASP H H 8.518 0.02 1 38 . 11 ASP C C 174.492 0.10 1 39 . 11 ASP CA C 54.319 0.10 1 40 . 11 ASP N N 125.724 0.20 1 41 . 12 TRP H H 7.150 0.02 1 42 . 12 TRP C C 172.289 0.10 1 43 . 12 TRP CA C 55.480 0.10 1 44 . 12 TRP N N 119.162 0.20 1 45 . 13 ASP H H 8.339 0.02 1 46 . 13 ASP C C 176.368 0.10 1 47 . 13 ASP CA C 53.145 0.10 1 48 . 13 ASP N N 119.709 0.20 1 49 . 14 ILE H H 8.402 0.02 1 50 . 14 ILE C C 172.794 0.10 1 51 . 14 ILE CA C 62.998 0.10 1 52 . 14 ILE N N 126.008 0.20 1 53 . 15 ASN H H 8.087 0.02 1 54 . 15 ASN C C 175.552 0.10 1 55 . 15 ASN CA C 53.396 0.10 1 56 . 15 ASN N N 115.493 0.20 1 57 . 16 ASP H H 7.930 0.02 1 58 . 16 ASP C C 176.229 0.10 1 59 . 16 ASP CA C 55.063 0.10 1 60 . 16 ASP N N 121.759 0.20 1 61 . 17 SER H H 8.654 0.02 1 62 . 17 SER C C 175.737 0.10 1 63 . 17 SER CA C 61.521 0.10 1 64 . 17 SER N N 115.607 0.20 1 65 . 18 LYS H H 7.711 0.02 1 66 . 18 LYS C C 174.215 0.10 1 67 . 18 LYS CA C 54.040 0.10 1 68 . 18 LYS N N 118.751 0.20 1 69 . 19 MET H H 8.443 0.02 1 70 . 19 MET C C 2.200 0.10 1 71 . 19 MET CA C 52.329 0.10 1 72 . 19 MET N N 118.615 0.20 1 73 . 20 PRO C C 176.735 0.10 1 74 . 20 PRO CA C 62.154 0.10 1 75 . 21 SER H H 8.361 0.02 1 76 . 21 SER C C 174.230 0.10 1 77 . 21 SER CA C 57.184 0.10 1 78 . 21 SER N N 118.068 0.20 1 79 . 22 VAL H H 8.233 0.02 1 80 . 22 VAL C C 175.101 0.10 1 81 . 22 VAL CA C 59.912 0.10 1 82 . 22 VAL N N 118.888 0.20 1 83 . 23 GLY H H 8.284 0.02 1 84 . 23 GLY C C 172.881 0.10 1 85 . 23 GLY CA C 44.450 0.10 1 86 . 23 GLY N N 107.267 0.20 1 87 . 24 GLU H H 6.674 0.02 1 88 . 24 GLU C C 172.881 0.10 1 89 . 24 GLU CA C 54.609 0.10 1 90 . 24 GLU N N 118.751 0.20 1 91 . 25 PHE H H 8.113 0.02 1 92 . 25 PHE C C 176.621 0.10 1 93 . 25 PHE CA C 57.601 0.10 1 94 . 25 PHE N N 117.384 0.20 1 95 . 26 ASP H H 9.466 0.02 1 96 . 26 ASP C C 175.760 0.10 1 97 . 26 ASP CA C 55.740 0.10 1 98 . 26 ASP N N 121.212 0.20 1 99 . 27 ASP H H 8.476 0.02 1 100 . 27 ASP C C 175.925 0.10 1 101 . 27 ASP CA C 53.970 0.10 1 102 . 27 ASP N N 120.529 0.20 1 103 . 28 PHE H H 8.669 0.02 1 104 . 28 PHE C C 174.339 0.10 1 105 . 28 PHE CA C 61.181 0.10 1 106 . 28 PHE N N 122.443 0.20 1 107 . 29 ASN H H 6.175 0.02 1 108 . 29 ASN C C 173.708 0.10 1 109 . 29 ASN CA C 50.821 0.10 1 110 . 29 ASN N N 125.724 0.20 1 111 . 30 ASP H H 8.722 0.02 1 112 . 30 ASP C C 177.538 0.10 1 113 . 30 ASP CA C 56.349 0.10 1 114 . 30 ASP N N 123.942 0.20 1 115 . 31 TRP H H 8.066 0.02 1 116 . 31 TRP C C 175.710 0.10 1 117 . 31 TRP CA C 53.965 0.10 1 118 . 31 TRP N N 116.974 0.20 1 119 . 32 SER H H 6.908 0.02 1 120 . 32 SER C C 174.278 0.10 1 121 . 32 SER CA C 59.344 0.10 1 122 . 32 SER N N 116.427 0.20 1 123 . 33 ASN H H 9.013 0.02 1 124 . 33 ASN C C 174.013 0.10 1 125 . 33 ASN CA C 51.679 0.10 1 126 . 33 ASN N N 124.220 0.20 1 127 . 34 GLY H H 7.367 0.02 1 128 . 34 GLY C C 173.534 0.10 1 129 . 34 GLY CA C 44.381 0.10 1 130 . 34 GLY N N 109.591 0.20 1 131 . 35 HIS H H 8.140 0.02 1 132 . 35 HIS C C 173.189 0.10 1 133 . 35 HIS CA C 55.683 0.10 1 134 . 35 HIS N N 117.380 0.20 1 135 . 36 CYS H H 7.977 0.02 1 136 . 36 CYS C C 171.562 0.10 1 137 . 36 CYS CA C 55.856 0.10 1 138 . 36 CYS N N 120.232 0.20 1 139 . 37 ARG H H 7.907 0.02 1 140 . 37 ARG C C 174.861 0.10 1 141 . 37 ARG CA C 55.307 0.10 1 142 . 37 ARG N N 119.982 0.20 1 143 . 38 LEU H H 9.654 0.02 1 144 . 38 LEU C C 173.949 0.10 1 145 . 38 LEU CA C 54.145 0.10 1 146 . 38 LEU N N 125.041 0.20 1 147 . 39 ILE H H 9.064 0.02 1 148 . 39 ILE C C 174.215 0.10 1 149 . 39 ILE CA C 61.045 0.10 1 150 . 39 ILE N N 118.888 0.20 1 151 . 40 TYR H H 9.245 0.02 1 152 . 40 TYR C C 175.253 0.10 1 153 . 40 TYR CA C 53.459 0.10 1 154 . 40 TYR N N 125.861 0.20 1 155 . 41 SER H H 8.653 0.02 1 156 . 41 SER C C 176.842 0.10 1 157 . 41 SER CA C 56.755 0.10 1 158 . 41 SER N N 113.829 0.20 1 159 . 42 VAL H H 7.457 0.02 1 160 . 42 VAL C C 175.471 0.10 1 161 . 42 VAL CA C 63.435 0.10 1 162 . 42 VAL N N 121.076 0.20 1 163 . 43 GLN H H 7.954 0.02 1 164 . 43 GLN C C 176.320 0.10 1 165 . 43 GLN CA C 55.972 0.10 1 166 . 43 GLN N N 116.427 0.20 1 167 . 44 SER H H 7.299 0.02 1 168 . 44 SER C C 174.544 0.10 1 169 . 44 SER CA C 56.806 0.10 1 170 . 44 SER N N 113.829 0.20 1 171 . 45 ASP H H 8.659 0.02 1 172 . 45 ASP C C 177.560 0.10 1 173 . 45 ASP CA C 57.190 0.10 1 174 . 45 ASP N N 131.740 0.20 1 175 . 46 GLU H H 8.013 0.02 1 176 . 46 GLU C C 177.234 0.10 1 177 . 46 GLU CA C 59.268 0.10 1 178 . 46 GLU N N 116.564 0.20 1 179 . 47 ALA H H 7.075 0.02 1 180 . 47 ALA C C 180.085 0.10 1 181 . 47 ALA CA C 54.533 0.10 1 182 . 47 ALA N N 119.572 0.20 1 183 . 48 ARG H H 7.770 0.02 1 184 . 48 ARG C C 177.604 0.10 1 185 . 48 ARG CA C 59.268 0.10 1 186 . 48 ARG N N 116.427 0.20 1 187 . 49 LYS H H 6.883 0.02 1 188 . 49 LYS C C 174.970 0.10 1 189 . 49 LYS CA C 55.328 0.10 1 190 . 49 LYS N N 114.240 0.20 1 191 . 50 HIS H H 7.179 0.02 1 192 . 50 HIS C C 173.621 0.10 1 193 . 50 HIS CA C 53.624 0.10 1 194 . 50 HIS N N 118.068 0.20 1 195 . 51 ALA H H 7.195 0.02 1 196 . 51 ALA C C 176.537 0.10 1 197 . 51 ALA CA C 52.563 0.10 1 198 . 51 ALA N N 118.888 0.20 1 199 . 52 SER H H 7.670 0.02 1 200 . 52 SER C C 173.974 0.10 1 201 . 52 SER CA C 58.510 0.10 1 202 . 52 SER N N 109.318 0.20 1 203 . 53 GLY H H 8.925 0.02 1 204 . 53 GLY C C 175.819 0.10 1 205 . 53 GLY CA C 45.059 0.10 1 206 . 53 GLY N N 115.197 0.20 1 207 . 54 TRP H H 7.279 0.02 1 208 . 54 TRP C C 175.291 0.10 1 209 . 54 TRP CA C 55.645 0.10 1 210 . 54 TRP N N 120.666 0.20 1 211 . 55 ALA H H 9.357 0.02 1 212 . 55 ALA C C 173.164 0.10 1 213 . 55 ALA CA C 51.476 0.10 1 214 . 55 ALA N N 127.228 0.20 1 215 . 56 MET H H 7.385 0.02 1 216 . 56 MET C C 175.481 0.10 1 217 . 56 MET CA C 51.730 0.10 1 218 . 56 MET N N 121.486 0.20 1 219 . 57 ARG H H 9.199 0.02 1 220 . 57 ARG C C 2.200 0.10 1 221 . 57 ARG CA C 53.622 0.10 1 222 . 57 ARG N N 121.896 0.20 1 223 . 58 ASN C C 2.200 0.10 1 224 . 58 ASN CA C 2.200 0.10 1 225 . 59 THR C C 174.752 0.10 1 226 . 59 THR CA C 61.503 0.10 1 227 . 60 ASN H H 7.727 0.02 1 228 . 60 ASN C C 174.937 0.10 1 229 . 60 ASN CA C 54.038 0.10 1 230 . 60 ASN N N 118.715 0.20 1 231 . 61 ASN H H 8.348 0.02 1 232 . 61 ASN C C 174.325 0.10 1 233 . 61 ASN CA C 51.540 0.10 1 234 . 61 ASN N N 122.306 0.20 1 235 . 62 HIS C C 174.448 0.10 1 236 . 62 HIS CA C 57.403 0.10 1 237 . 63 ASN H H 7.323 0.02 1 238 . 63 ASN C C 175.861 0.10 1 239 . 63 ASN CA C 52.108 0.10 1 240 . 63 ASN N N 117.384 0.20 1 241 . 64 VAL H H 8.295 0.02 1 242 . 64 VAL C C 176.127 0.10 1 243 . 64 VAL CA C 63.435 0.10 1 244 . 64 VAL N N 120.666 0.20 1 245 . 65 ASN H H 8.426 0.02 1 246 . 65 ASN C C 174.186 0.10 1 247 . 65 ASN CA C 54.002 0.10 1 248 . 65 ASN N N 116.290 0.20 1 249 . 66 ILE H H 7.543 0.02 1 250 . 66 ILE C C 174.506 0.10 1 251 . 66 ILE CA C 60.306 0.10 1 252 . 66 ILE N N 119.005 0.20 1 253 . 67 LEU H H 8.892 0.02 1 254 . 67 LEU C C 174.075 0.10 1 255 . 67 LEU CA C 54.377 0.10 1 256 . 67 LEU N N 130.646 0.20 1 257 . 68 LYS H H 8.919 0.02 1 258 . 68 LYS C C 174.987 0.10 1 259 . 68 LYS CA C 53.825 0.10 1 260 . 68 LYS N N 129.006 0.20 1 261 . 69 LYS H H 9.070 0.02 1 262 . 69 LYS C C 174.696 0.10 1 263 . 69 LYS CA C 54.201 0.10 1 264 . 69 LYS N N 122.716 0.20 1 265 . 70 SER H H 8.945 0.02 1 266 . 70 SER C C 173.469 0.10 1 267 . 70 SER CA C 56.349 0.10 1 268 . 70 SER N N 113.419 0.20 1 269 . 71 CYS H H 8.044 0.02 1 270 . 71 CYS C C 177.571 0.10 1 271 . 71 CYS CA C 60.972 0.10 1 272 . 71 CYS N N 125.998 0.20 1 273 . 72 LEU H H 9.316 0.02 1 274 . 72 LEU C C 176.216 0.10 1 275 . 72 LEU CA C 54.986 0.10 1 276 . 72 LEU N N 130.136 0.20 1 277 . 73 GLY H H 9.687 0.02 1 278 . 73 GLY C C 171.837 0.10 1 279 . 73 GLY CA C 43.037 0.10 1 280 . 73 GLY N N 112.052 0.20 1 281 . 74 VAL H H 7.374 0.02 1 282 . 74 VAL C C 174.013 0.10 1 283 . 74 VAL CA C 60.935 0.10 1 284 . 74 VAL N N 116.154 0.20 1 285 . 75 LEU H H 7.684 0.02 1 286 . 75 LEU C C 174.620 0.10 1 287 . 75 LEU CA C 58.510 0.10 1 288 . 75 LEU N N 124.220 0.20 1 289 . 76 LEU H H 8.951 0.02 1 290 . 76 LEU C C 173.099 0.10 1 291 . 76 LEU CA C 52.120 0.10 1 292 . 76 LEU N N 125.314 0.20 1 293 . 77 CYS H H 6.355 0.02 1 294 . 77 CYS C C 179.471 0.10 1 295 . 77 CYS CA C 51.351 0.10 1 296 . 77 CYS N N 110.685 0.20 1 297 . 78 SER H H 9.647 0.02 1 298 . 78 SER C C 174.506 0.10 1 299 . 78 SER CA C 61.704 0.10 1 300 . 78 SER N N 129.416 0.20 1 301 . 79 ALA H H 9.689 0.02 1 302 . 79 ALA C C 176.951 0.10 1 303 . 79 ALA CA C 51.621 0.10 1 304 . 79 ALA N N 128.869 0.20 1 305 . 80 LYS H H 8.261 0.02 1 306 . 80 LYS C C 175.602 0.10 1 307 . 80 LYS CA C 56.995 0.10 1 308 . 80 LYS N N 117.248 0.20 1 309 . 81 CYS H H 6.829 0.02 1 310 . 81 CYS C C 174.949 0.10 1 311 . 81 CYS CA C 57.828 0.10 1 312 . 81 CYS N N 116.017 0.20 1 313 . 82 LYS H H 8.158 0.02 1 314 . 82 LYS C C 176.431 0.10 1 315 . 82 LYS CA C 54.533 0.10 1 316 . 82 LYS N N 117.521 0.20 1 317 . 83 LEU H H 8.730 0.02 1 318 . 83 LEU C C 177.011 0.10 1 319 . 83 LEU CA C 53.796 0.10 1 320 . 83 LEU N N 123.947 0.20 1 321 . 84 PRO C C 176.516 0.10 1 322 . 84 PRO CA C 65.408 0.10 1 323 . 85 ASN H H 7.517 0.02 1 324 . 85 ASN C C 176.317 0.10 1 325 . 85 ASN CA C 52.418 0.10 1 326 . 85 ASN N N 111.642 0.20 1 327 . 86 GLY H H 8.424 0.02 1 328 . 86 GLY C C 173.469 0.10 1 329 . 86 GLY CA C 44.911 0.10 1 330 . 86 GLY N N 108.361 0.20 1 331 . 87 ALA H H 7.465 0.02 1 332 . 87 ALA C C 175.797 0.10 1 333 . 87 ALA CA C 51.616 0.10 1 334 . 87 ALA N N 122.580 0.20 1 335 . 88 SER H H 8.125 0.02 1 336 . 88 SER C C 172.910 0.10 1 337 . 88 SER CA C 57.109 0.10 1 338 . 88 SER N N 113.283 0.20 1 339 . 89 VAL H H 8.715 0.02 1 340 . 89 VAL C C 175.570 0.10 1 341 . 89 VAL CA C 63.831 0.10 1 342 . 89 VAL N N 122.990 0.20 1 343 . 90 HIS H H 8.730 0.02 1 344 . 90 HIS C C 174.329 0.10 1 345 . 90 HIS CA C 52.593 0.10 1 346 . 90 HIS N N 128.869 0.20 1 347 . 91 LEU H H 8.520 0.02 1 348 . 91 LEU C C 176.089 0.10 1 349 . 91 LEU CA C 54.192 0.10 1 350 . 91 LEU N N 122.033 0.20 1 351 . 92 ARG H H 8.779 0.02 1 352 . 92 ARG C C 2.200 0.10 1 353 . 92 ARG CA C 54.319 0.10 1 354 . 92 ARG N N 122.443 0.20 1 355 . 93 PRO C C 174.861 0.10 1 356 . 93 PRO CA C 61.764 0.10 1 357 . 94 ALA H H 9.234 0.02 1 358 . 94 ALA C C 176.543 0.10 1 359 . 94 ALA CA C 51.450 0.10 1 360 . 94 ALA N N 126.271 0.20 1 361 . 95 ILE H H 7.867 0.02 1 362 . 95 ILE C C 177.016 0.10 1 363 . 95 ILE CA C 63.005 0.10 1 364 . 95 ILE N N 120.157 0.20 1 365 . 96 CYS H H 7.664 0.02 1 366 . 96 CYS C C 176.429 0.10 1 367 . 96 CYS CA C 59.684 0.10 1 368 . 96 CYS N N 122.033 0.20 1 369 . 97 ASP H H 8.475 0.02 1 370 . 97 ASP C C 178.635 0.10 1 371 . 97 ASP CA C 57.639 0.10 1 372 . 97 ASP N N 129.663 0.20 1 373 . 98 LYS H H 8.989 0.02 1 374 . 98 LYS C C 179.018 0.10 1 375 . 98 LYS CA C 59.481 0.10 1 376 . 98 LYS N N 122.990 0.20 1 377 . 99 ALA H H 8.371 0.02 1 378 . 99 ALA C C 175.295 0.10 1 379 . 99 ALA CA C 54.251 0.10 1 380 . 99 ALA N N 125.583 0.20 1 381 . 100 ARG H H 8.591 0.02 1 382 . 100 ARG C C 179.454 0.10 1 383 . 100 ARG CA C 58.292 0.10 1 384 . 100 ARG N N 120.529 0.20 1 385 . 101 ARG H H 7.507 0.02 1 386 . 101 ARG C C 178.605 0.10 1 387 . 101 ARG CA C 58.927 0.10 1 388 . 101 ARG N N 118.341 0.20 1 389 . 102 LYS H H 7.102 0.02 1 390 . 102 LYS C C 177.582 0.10 1 391 . 102 LYS CA C 57.525 0.10 1 392 . 102 LYS N N 115.607 0.20 1 393 . 103 GLN H H 7.437 0.02 1 394 . 103 GLN C C 176.603 0.10 1 395 . 103 GLN CA C 58.283 0.10 1 396 . 103 GLN N N 116.837 0.20 1 397 . 104 GLN H H 6.801 0.02 1 398 . 104 GLN C C 175.896 0.10 1 399 . 104 GLN CA C 56.843 0.10 1 400 . 104 GLN N N 114.923 0.20 1 401 . 105 GLY H H 9.084 0.02 1 402 . 105 GLY C C 174.187 0.10 1 403 . 105 GLY CA C 45.154 0.10 1 404 . 105 GLY N N 113.556 0.20 1 405 . 106 LYS H H 7.241 0.02 1 406 . 106 LYS C C 175.190 0.10 1 407 . 106 LYS CA C 55.897 0.10 1 408 . 106 LYS N N 121.076 0.20 1 409 . 107 GLN H H 8.403 0.02 1 410 . 107 GLN C C 174.230 0.10 1 411 . 107 GLN CA C 56.995 0.10 1 412 . 107 GLN N N 120.802 0.20 1 413 . 108 CYS H H 7.591 0.02 1 414 . 108 CYS C C 2.200 0.10 1 415 . 108 CYS CA C 57.525 0.10 1 416 . 108 CYS N N 125.861 0.20 1 417 . 109 PRO C C 176.938 0.10 1 418 . 109 PRO CA C 63.553 0.10 1 419 . 110 ASN H H 9.291 0.02 1 420 . 110 ASN C C 175.494 0.10 1 421 . 110 ASN CA C 52.723 0.10 1 422 . 110 ASN N N 123.947 0.20 1 423 . 111 ARG H H 8.349 0.02 1 424 . 111 ARG C C 176.755 0.10 1 425 . 111 ARG CA C 57.715 0.10 1 426 . 111 ARG N N 125.861 0.20 1 427 . 112 ASN H H 7.987 0.02 1 428 . 112 ASN C C 172.859 0.10 1 429 . 112 ASN CA C 53.018 0.10 1 430 . 112 ASN N N 115.880 0.20 1 431 . 113 CYS H H 7.688 0.02 1 432 . 113 CYS C C 174.578 0.10 1 433 . 113 CYS CA C 58.823 0.10 1 434 . 113 CYS N N 123.537 0.20 1 435 . 114 ASN H H 8.064 0.02 1 436 . 114 ASN C C 175.688 0.10 1 437 . 114 ASN CA C 52.525 0.10 1 438 . 114 ASN N N 125.041 0.20 1 439 . 115 GLY H H 10.208 0.02 1 440 . 115 GLY C C 171.998 0.10 1 441 . 115 GLY CA C 44.081 0.10 1 442 . 115 GLY N N 116.154 0.20 1 443 . 116 ARG H H 8.462 0.02 1 444 . 116 ARG C C 175.608 0.10 1 445 . 116 ARG CA C 52.639 0.10 1 446 . 116 ARG N N 116.427 0.20 1 447 . 117 LEU H H 8.977 0.02 1 448 . 117 LEU C C 177.665 0.10 1 449 . 117 LEU CA C 54.261 0.10 1 450 . 117 LEU N N 119.298 0.20 1 451 . 118 GLU H H 9.376 0.02 1 452 . 118 GLU C C 175.051 0.10 1 453 . 118 GLU CA C 53.825 0.10 1 454 . 118 GLU N N 121.486 0.20 1 455 . 119 ILE H H 8.875 0.02 1 456 . 119 ILE C C 175.448 0.10 1 457 . 119 ILE CA C 60.757 0.10 1 458 . 119 ILE N N 124.084 0.20 1 459 . 120 GLN H H 9.038 0.02 1 460 . 120 GLN C C 173.534 0.10 1 461 . 120 GLN CA C 54.754 0.10 1 462 . 120 GLN N N 130.510 0.20 1 463 . 121 ALA H H 8.063 0.02 1 464 . 121 ALA C C 175.380 0.10 1 465 . 121 ALA CA C 51.351 0.10 1 466 . 121 ALA N N 127.638 0.20 1 467 . 122 CYS H H 8.653 0.02 1 468 . 122 CYS C C 175.639 0.10 1 469 . 122 CYS CA C 59.626 0.10 1 470 . 122 CYS N N 123.400 0.20 1 471 . 123 ARG H H 9.943 0.02 1 472 . 123 ARG C C 178.278 0.10 1 473 . 123 ARG CA C 54.870 0.10 1 474 . 123 ARG N N 130.236 0.20 1 475 . 124 GLY H H 7.633 0.02 1 476 . 124 GLY C C 173.556 0.10 1 477 . 124 GLY CA C 47.146 0.10 1 478 . 124 GLY N N 105.216 0.20 1 479 . 125 HIS H H 9.389 0.02 1 480 . 125 HIS C C 175.076 0.10 1 481 . 125 HIS CA C 51.937 0.10 1 482 . 125 HIS N N 122.443 0.20 1 483 . 126 CYS H H 8.570 0.02 1 484 . 126 CYS C C 173.904 0.10 1 485 . 126 CYS CA C 60.293 0.10 1 486 . 126 CYS N N 116.564 0.20 1 487 . 127 GLY H H 7.217 0.02 1 488 . 127 GLY C C 2.200 0.10 1 489 . 127 GLY CA C 44.949 0.10 1 490 . 127 GLY N N 102.618 0.20 1 491 . 128 TYR C C 2.200 0.10 1 492 . 128 TYR CA C 2.200 0.10 1 493 . 129 PRO C C 176.429 0.10 1 494 . 129 PRO CA C 63.553 0.10 1 495 . 130 VAL H H 8.157 0.02 1 496 . 130 VAL C C 174.513 0.10 1 497 . 130 VAL CA C 64.003 0.10 1 498 . 130 VAL N N 123.947 0.20 1 499 . 131 THR H H 7.361 0.02 1 500 . 131 THR C C 172.948 0.10 1 501 . 131 THR CA C 58.245 0.10 1 502 . 131 THR N N 115.197 0.20 1 503 . 132 HIS H H 8.373 0.02 1 504 . 132 HIS C C 173.429 0.10 1 505 . 132 HIS CA C 51.878 0.10 1 506 . 132 HIS N N 119.918 0.20 1 507 . 133 PHE H H 8.591 0.02 1 508 . 133 PHE C C 173.531 0.10 1 509 . 133 PHE CA C 55.649 0.10 1 510 . 133 PHE N N 117.248 0.20 1 511 . 134 TRP H H 9.024 0.02 1 512 . 134 TRP C C 176.457 0.10 1 513 . 134 TRP CA C 55.044 0.10 1 514 . 134 TRP N N 118.751 0.20 1 515 . 135 ARG H H 9.623 0.02 1 516 . 135 ARG C C 173.493 0.10 1 517 . 135 ARG CA C 55.131 0.10 1 518 . 135 ARG N N 124.220 0.20 1 519 . 136 ARG H H 9.382 0.02 1 520 . 136 ARG C C 175.371 0.10 1 521 . 136 ARG CA C 55.375 0.10 1 522 . 136 ARG N N 128.459 0.20 1 523 . 137 ASP H H 9.053 0.02 1 524 . 137 ASP C C 175.342 0.10 1 525 . 137 ASP CA C 54.580 0.10 1 526 . 137 ASP N N 127.092 0.20 1 527 . 138 GLY H H 8.817 0.02 1 528 . 138 GLY C C 174.759 0.10 1 529 . 138 GLY CA C 47.119 0.10 1 530 . 138 GLY N N 112.599 0.20 1 531 . 139 ASN H H 8.874 0.02 1 532 . 139 ASN C C 174.687 0.10 1 533 . 139 ASN CA C 52.630 0.10 1 534 . 139 ASN N N 122.853 0.20 1 535 . 140 GLY H H 8.210 0.02 1 536 . 140 GLY C C 170.727 0.10 1 537 . 140 GLY CA C 44.646 0.10 1 538 . 140 GLY N N 108.224 0.20 1 539 . 141 ILE H H 8.926 0.02 1 540 . 141 ILE C C 172.999 0.10 1 541 . 141 ILE CA C 61.072 0.10 1 542 . 141 ILE N N 121.076 0.20 1 543 . 142 TYR H H 9.559 0.02 1 544 . 142 TYR C C 173.429 0.10 1 545 . 142 TYR CA C 57.944 0.10 1 546 . 142 TYR N N 128.322 0.20 1 547 . 143 PHE H H 9.044 0.02 1 548 . 143 PHE C C 172.642 0.10 1 549 . 143 PHE CA C 55.479 0.10 1 550 . 143 PHE N N 123.673 0.20 1 551 . 144 GLN H H 8.134 0.02 1 552 . 144 GLN C C 171.757 0.10 1 553 . 144 GLN CA C 53.321 0.10 1 554 . 144 GLN N N 126.955 0.20 1 555 . 145 ALA H H 8.367 0.02 1 556 . 145 ALA C C 175.773 0.10 1 557 . 145 ALA CA C 49.504 0.10 1 558 . 145 ALA N N 125.588 0.20 1 559 . 146 LYS H H 8.356 0.02 1 560 . 146 LYS C C 996.253 0.10 1 561 . 146 LYS CA C 55.168 0.10 1 562 . 146 LYS N N 118.473 0.20 1 563 . 147 GLY C C 175.730 0.10 1 564 . 147 GLY CA C 43.028 0.10 1 565 . 148 THR H H 7.951 0.02 1 566 . 148 THR C C 170.574 0.10 1 567 . 148 THR CA C 59.940 0.10 1 568 . 148 THR N N 119.982 0.20 1 569 . 149 HIS H H 8.337 0.02 1 570 . 149 HIS C C 174.126 0.10 1 571 . 149 HIS CA C 54.571 0.10 1 572 . 149 HIS N N 126.408 0.20 1 573 . 150 ASP H H 8.585 0.02 1 574 . 150 ASP C C 180.722 0.10 1 575 . 150 ASP CA C 52.276 0.10 1 576 . 150 ASP N N 122.055 0.20 1 577 . 151 HIS H H 8.578 0.02 1 578 . 151 HIS C C 2.200 0.10 1 579 . 151 HIS CA C 59.452 0.10 1 580 . 151 HIS N N 120.529 0.20 1 581 . 152 PRO C C 176.444 0.10 1 582 . 152 PRO CA C 62.175 0.10 1 583 . 153 ARG H H 8.481 0.02 1 584 . 153 ARG C C 174.815 0.10 1 585 . 153 ARG CA C 54.457 0.10 1 586 . 153 ARG N N 122.033 0.20 1 587 . 154 PRO C C 176.722 0.10 1 588 . 154 PRO CA C 61.684 0.10 1 589 . 155 GLU H H 7.694 0.02 1 590 . 155 GLU C C 175.646 0.10 1 591 . 155 GLU CA C 56.578 0.10 1 592 . 155 GLU N N 119.008 0.20 1 593 . 156 ALA H H 8.445 0.02 1 594 . 156 ALA C C 177.229 0.10 1 595 . 156 ALA CA C 52.454 0.10 1 596 . 156 ALA N N 124.494 0.20 1 597 . 157 LYS H H 8.271 0.02 1 598 . 157 LYS C C 176.469 0.10 1 599 . 157 LYS CA C 56.351 0.10 1 600 . 157 LYS N N 119.572 0.20 1 601 . 158 GLY H H 8.423 0.02 1 602 . 158 GLY C C 173.055 0.10 1 603 . 158 GLY CA C 45.139 0.10 1 604 . 158 GLY N N 110.548 0.20 1 605 . 159 SER H H 7.778 0.02 1 606 . 159 SER C C 178.469 0.10 1 607 . 159 SER CA C 59.836 0.10 1 608 . 159 SER N N 120.802 0.20 1 stop_ save_