data_6898 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR structure of the peptide linked to the genome (VPg) of poliovirus ; _BMRB_accession_number 6898 _BMRB_flat_file_name bmr6898.str _Entry_type original _Submission_date 2005-11-10 _Accession_date 2005-11-29 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Schein C. H. . 2 Oezguen N. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 228 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2007-02-06 original author . stop_ _Original_release_date 2007-02-06 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'NMR structure of the viral peptide linked to the genome (VPg) of poliovirus' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16540201 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Schein C. H. . 2 Oezguen N. N. . 3 Volk D. E. . 4 Garimella R. . . 5 Paul A. . . 6 Braun W. . . stop_ _Journal_abbreviation Peptides _Journal_volume 27 _Journal_issue 7 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1676 _Page_last 1684 _Year 2006 _Details . loop_ _Keyword 'peptide primer' 'polymerase cofactor' 'RNA synthesis' uridylylation 'viral replication' stop_ save_ ################################## # Molecular system description # ################################## save_system _Saveframe_category molecular_system _Mol_system_name 'Genome linked protein VPg' _Abbreviation_common 'Genome linked protein VPg' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Genome linked protein VPg' $VPg stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_VPg _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Genome linked protein VPg' _Abbreviation_common 'Genome linked protein VPg' _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 22 _Mol_residue_sequence ; GAYTGLPNKKPNVPTIRTAK VQ ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 ALA 3 TYR 4 THR 5 GLY 6 LEU 7 PRO 8 ASN 9 LYS 10 LYS 11 PRO 12 ASN 13 VAL 14 PRO 15 THR 16 ILE 17 ARG 18 THR 19 ALA 20 LYS 21 VAL 22 GLN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2BBL "Nmr Structures Of The Peptide Linked To The Genome (Vpg) Of Poliovirus In A Stabilizing Solvent" 100.00 22 100.00 100.00 1.60e-05 PDB 2BBP "Nmr Structures Of The Peptide Linked To The Genome (Vpg) Of Poliovirus" 100.00 22 100.00 100.00 1.60e-05 DBJ BAD72853 "polyprotein [Human poliovirus 1]" 100.00 2209 100.00 100.00 1.01e-04 DBJ BAD72854 "polyprotein [Human poliovirus 1]" 100.00 2209 100.00 100.00 1.01e-04 DBJ BAD72855 "polyprotein [Human poliovirus 1]" 100.00 2209 100.00 100.00 1.01e-04 DBJ BAD72856 "polyprotein [Human poliovirus 1]" 100.00 2209 100.00 100.00 1.01e-04 DBJ BAE20395 "polyprotein [Coxsackievirus A17]" 100.00 960 100.00 100.00 7.90e-05 EMBL CAA24445 "unnamed protein product [Human poliovirus 1]" 100.00 2221 100.00 100.00 9.44e-05 EMBL CAA24446 "unnamed protein product [Human poliovirus 1]" 100.00 2207 100.00 100.00 9.61e-05 EMBL CAA24456 "unnamed protein product [Human poliovirus 1]" 100.00 752 100.00 100.00 7.65e-05 EMBL CAA24457 "unnamed protein product [Human poliovirus 1]" 100.00 22 100.00 100.00 1.60e-05 EMBL CAA24461 "polyprotein [Human poliovirus 1 Mahoney]" 100.00 2209 100.00 100.00 1.03e-04 GB AAA46911 "P3-1b polyprotein precursor, partial [Human poliovirus 1]" 100.00 757 100.00 100.00 7.52e-05 GB AAG27151 "precursor polyprotein [Human poliovirus 1]" 100.00 2209 100.00 100.00 9.98e-05 GB AAG27168 "precursor polyprotein [Human poliovirus 1]" 100.00 2209 100.00 100.00 1.01e-04 GB AAN85442 "polyprotein [Human poliovirus 1]" 100.00 2209 100.00 100.00 1.03e-04 GB AAP37263 "polyprotein precursor [Human poliovirus 2]" 100.00 2207 100.00 100.00 1.05e-04 PRF 0707268A polyprotein 100.00 2221 100.00 100.00 9.44e-05 PRF 0908283A "protein P3-9" 100.00 109 100.00 100.00 1.35e-05 PRF 0908283B "protein VPg" 100.00 22 100.00 100.00 1.60e-05 REF NP_041277 "genome polyprotein [Enterovirus C]" 100.00 2209 100.00 100.00 1.03e-04 REF NP_740475 "genome linked protein VPg [Human enterovirus C]" 100.00 22 100.00 100.00 1.60e-05 REF YP_007353733 "protein 3AB [Human enterovirus C]" 100.00 109 100.00 100.00 1.35e-05 SP P03300 "RecName: Full=Genome polyprotein; Contains: RecName: Full=P1; Contains: RecName: Full=Capsid protein VP0; AltName: Full=VP4-VP2" 100.00 2209 100.00 100.00 1.03e-04 SP P03301 "RecName: Full=Genome polyprotein; Contains: RecName: Full=P3; Contains: RecName: Full=Protein 3AB; Contains: RecName: Full=P1; " 100.00 2209 100.00 100.00 1.03e-04 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $VPg Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $VPg 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $VPg 2.78 mM . 'sodium phosphate buffer' 0.01 M . 'deuterated Trimethylamine oxide' 1 M . D2O 10 % . H2O 90 % . stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $VPg 3.7 mM . 'sodium phosphate buffer' 0.01 M . D2O 10 % . H2O 90 % . stop_ save_ ############################ # Computer software used # ############################ save_NOAH _Saveframe_category software _Name NOAH _Version 2.0 loop_ _Task 'structure solution' stop_ _Details 'Mumenthaler, C. et al' save_ save_DIAMOD _Saveframe_category software _Name DIAMOD _Version 2.2 loop_ _Task refinement 'structure solution' stop_ _Details 'Guentert, P. et al' save_ save_FELIX _Saveframe_category software _Name FELIX _Version . loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model UNITYplus _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_TOCSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _Sample_label . save_ save_2D_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label . save_ save_DQF-COSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 10 . mM pH 7.2 . pH pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis . H 1 . ppm . . . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D TOCSY' '2D NOESY' DQF-COSY stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'Genome linked protein VPg' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 GLY H H 8.803 . . 2 . 1 GLY HA2 H 3.55 . . 3 . 1 GLY HA3 H 3.65 . . 4 . 2 ALA H H 8.568 . . 5 . 2 ALA HA H 4.215 . . 6 . 2 ALA HB H 1.216 . . 7 . 3 TYR H H 8.46 . . 8 . 3 TYR HA H 4.566 . . 9 . 3 TYR HB2 H 3.02 . . 10 . 3 TYR HB3 H 2.95 . . 11 . 3 TYR HD1 H 7.07 . . 12 . 3 TYR HD2 H 7.07 . . 13 . 3 TYR HE1 H 6.72 . . 14 . 3 TYR HE2 H 6.72 . . 15 . 4 THR H H 8.08 . . 16 . 4 THR HA H 4.19 . . 17 . 4 THR HB H 4.12 . . 18 . 4 THR HG2 H 1.075 . . 19 . 5 GLY H H 7.4 . . 20 . 5 GLY HA2 H 3.77 . . 21 . 6 LEU H H 8.093 . . 22 . 6 LEU HA H 4.52 . . 23 . 6 LEU HB2 H 1.50 . . 24 . 6 LEU HB3 H 1.50 . . 25 . 6 LEU HG H 1.47 . . 26 . 6 LEU HD1 H 0.86 . . 27 . 6 LEU HD2 H 0.835 . . 28 . 7 PRO HA H 4.31 . . 29 . 7 PRO HB2 H 2.22 . . 30 . 7 PRO HG2 H 1.94 . . 31 . 7 PRO HG3 H 1.81 . . 32 . 7 PRO HD2 H 3.76 . . 33 . 7 PRO HD3 H 3.59 . . 34 . 8 ASN H H 8.456 . . 35 . 8 ASN HA H 4.54 . . 36 . 8 ASN HB2 H 2.67 . . 37 . 8 ASN HD21 H 7.60 . . 38 . 8 ASN HD22 H 6.91 . . 39 . 9 LYS H H 8.18 . . 40 . 9 LYS HA H 4.215 . . 41 . 9 LYS HB2 H 1.74 . . 42 . 9 LYS HB3 H 1.64 . . 43 . 9 LYS HG2 H 1.31 . . 44 . 9 LYS HD2 H 1.63 . . 45 . 9 LYS HE2 H 3.14 . . 46 . 10 LYS H H 8.35 . . 47 . 10 LYS HA H 4.45 . . 48 . 10 LYS HB2 H 1.71 . . 49 . 10 LYS HG2 H 1.38 . . 50 . 10 LYS HD2 H 1.61 . . 51 . 10 LYS HE2 H 3.13 . . 52 . 11 PRO HA H 4.309 . . 53 . 11 PRO HB2 H 2.20 . . 54 . 11 PRO HB3 H 1.92 . . 55 . 11 PRO HG2 H 1.8 . . 56 . 11 PRO HD2 H 3.72 . . 57 . 12 ASN H H 8.56 . . 58 . 12 ASN HA H 4.57 . . 59 . 12 ASN HB2 H 2.68 . . 60 . 12 ASN HB3 H 2.64 . . 61 . 12 ASN HD21 H 7.62 . . 62 . 12 ASN HD22 H 6.93 . . 63 . 13 VAL H H 8.057 . . 64 . 13 VAL HA H 4.379 . . 65 . 13 VAL HB H 1.99 . . 66 . 13 VAL HG1 H 0.887 . . 67 . 13 VAL HG2 H 0.84 . . 68 . 14 PRO HA H 4.379 . . 69 . 14 PRO HB2 H 2.22 . . 70 . 14 PRO HB3 H 1.92 . . 71 . 14 PRO HG2 H 1.80 . . 72 . 14 PRO HD2 H 3.61 . . 73 . 14 PRO HD3 H 3.77 . . 74 . 15 THR H H 8.28 . . 75 . 15 THR HA H 4.18 . . 76 . 15 THR HB H 4.045 . . 77 . 15 THR HG2 H 1.122 . . 78 . 16 ILE H H 8.20 . . 79 . 16 ILE HA H 4.10 . . 80 . 16 ILE HB H 1.77 . . 81 . 16 ILE HG12 H 1.39 . . 82 . 16 ILE HG2 H 0.817 . . 83 . 16 ILE HD1 H 0.806 . . 84 . 17 ARG H H 8.50 . . 85 . 17 ARG HA H 4.33 . . 86 . 17 ARG HB2 H 1.78 . . 87 . 17 ARG HB3 H 1.68 . . 88 . 17 ARG HG2 H 1.57 . . 89 . 17 ARG HG3 H 1.52 . . 90 . 17 ARG HD2 H 3.11 . . 91 . 17 ARG HE H 7.16 . . 92 . 17 ARG HH11 H 6.700 . . 93 . 18 THR H H 8.134 . . 94 . 18 THR HA H 4.215 . . 95 . 18 THR HB H 4.10 . . 96 . 18 THR HG2 H 1.12 . . 97 . 19 ALA H H 8.30 . . 98 . 19 ALA HA H 4.238 . . 99 . 19 ALA HB H 1.286 . . 100 . 20 LYS H H 8.298 . . 101 . 20 LYS HA H 4.215 . . 102 . 20 LYS HB2 H 1.71 . . 103 . 20 LYS HB3 H 1.66 . . 104 . 20 LYS HG2 H 1.36 . . 105 . 20 LYS HD2 H 1.59 . . 106 . 20 LYS HE2 H 3.15 . . 107 . 21 VAL H H 8.25 . . 108 . 21 VAL HA H 4.00 . . 109 . 21 VAL HB H 1.99 . . 110 . 21 VAL HG1 H 0.864 . . 111 . 22 GLN H H 7.99 . . 112 . 22 GLN HA H 4.07 . . 113 . 22 GLN HB2 H 1.99 . . 114 . 22 GLN HB3 H 1.85 . . 115 . 22 GLN HG2 H 2.20 . . 116 . 22 GLN HE21 H 6.84 . . 117 . 22 GLN HE22 H 7.05 . . stop_ save_ save_chemical_shift_set_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_2 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'Genome linked protein VPg' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 2 ALA H H 8.386 . . 2 . 2 ALA HA H 4.27 . . 3 . 2 ALA HB H 1.33 . . 4 . 3 TYR H H 8.57 . . 5 . 3 TYR HA H 4.61 . . 6 . 3 TYR HB2 H 3.0 . . 7 . 3 TYR HB3 H 2.96 . . 8 . 3 TYR HD1 H 7.1 . . 9 . 3 TYR HD2 H 7.1 . . 10 . 3 TYR HE1 H 6.75 . . 11 . 3 TYR HE2 H 6.75 . . 12 . 4 THR H H 8.19 . . 13 . 4 THR HA H 4.23 . . 14 . 4 THR HB H 4.16 . . 15 . 4 THR HG2 H 1.10 . . 16 . 5 GLY H H 7.27 . . 17 . 5 GLY HA2 H 3.8 . . 18 . 6 LEU H H 8.20 . . 19 . 6 LEU HA H 4.57 . . 20 . 6 LEU HB2 H 1.57 . . 21 . 6 LEU HB3 H 1.55 . . 22 . 6 LEU HG H 1.52 . . 23 . 6 LEU HD1 H 0.9 . . 24 . 6 LEU HD2 H 0.88 . . 25 . 7 PRO HA H 4.362 . . 26 . 7 PRO HB2 H 2.32 . . 27 . 7 PRO HG2 H 1.97 . . 28 . 7 PRO HG3 H 1.84 . . 29 . 7 PRO HD2 H 3.81 . . 30 . 7 PRO HD3 H 3.61 . . 31 . 8 ASN H H 8.55 . . 32 . 8 ASN HA H 4.59 . . 33 . 8 ASN HB2 H 2.72 . . 34 . 8 ASN HD21 H 7.64 . . 35 . 8 ASN HD22 H 6.94 . . 36 . 9 LYS H H 8.29 . . 37 . 9 LYS HA H 4.26 . . 38 . 9 LYS HB2 H 1.77 . . 39 . 9 LYS HB3 H 1.67 . . 40 . 9 LYS HG2 H 1.35 . . 41 . 9 LYS HD2 H 1.63 . . 42 . 9 LYS HE2 H 2.93 . . 43 . 10 LYS H H 8.43 . . 44 . 10 LYS HA H 4.50 . . 45 . 10 LYS HB2 H 1.77 . . 46 . 10 LYS HG2 H 1.40 . . 47 . 10 LYS HD2 H 1.65 . . 48 . 10 LYS HE2 H 2.95 . . 49 . 11 PRO HA H 4.35 . . 50 . 11 PRO HB2 H 2.23 . . 51 . 11 PRO HB3 H 1.96 . . 52 . 11 PRO HG2 H 1.83 . . 53 . 11 PRO HD2 H 3.77 . . 54 . 11 PRO HD3 H 3.59 . . 55 . 12 ASN H H 8.65 . . 56 . 12 ASN HA H 4.6 . . 57 . 12 ASN HB2 H 2.71 . . 58 . 12 ASN HB3 H 2.67 . . 59 . 12 ASN HD21 H 7.66 . . 60 . 12 ASN HD22 H 6.96 . . 61 . 13 VAL H H 8.19 . . 62 . 13 VAL HA H 4.41 . . 63 . 13 VAL HB H 2.03 . . 64 . 13 VAL HG1 H 0.92 . . 65 . 13 VAL HG2 H 0.87 . . 66 . 14 PRO HA H 4.42 . . 67 . 14 PRO HB2 H 2.25 . . 68 . 14 PRO HB3 H 1.98 . . 69 . 14 PRO HG2 H 1.83 . . 70 . 14 PRO HG3 H 1.94 . . 71 . 14 PRO HD2 H 3.81 . . 72 . 14 PRO HD3 H 3.66 . . 73 . 15 THR H H 8.395 . . 74 . 15 THR HA H 4.21 . . 75 . 15 THR HB H 4.07 . . 76 . 15 THR HG2 H 1.17 . . 77 . 16 ILE H H 8.31 . . 78 . 16 ILE HA H 4.13 . . 79 . 16 ILE HB H 1.79 . . 80 . 16 ILE HG12 H 1.42 . . 81 . 16 ILE HG13 H 1.12 . . 82 . 16 ILE HG2 H 0.84 . . 83 . 16 ILE HD1 H 0.80 . . 84 . 17 ARG H H 8.59 . . 85 . 17 ARG HA H 4.38 . . 86 . 17 ARG HB2 H 1.80 . . 87 . 17 ARG HB3 H 1.71 . . 88 . 17 ARG HG2 H 1.61 . . 89 . 17 ARG HG3 H 1.55 . . 90 . 17 ARG HD2 H 3.15 . . 91 . 18 THR H H 8.25 . . 92 . 18 THR HA H 4.25 . . 93 . 18 THR HB H 4.14 . . 94 . 18 THR HG2 H 1.16 . . 95 . 19 ALA H H 8.609 . . 96 . 19 ALA HA H 4.256 . . 97 . 19 ALA HB H 1.27 . . 98 . 20 LYS H H 8.384 . . 99 . 20 LYS HA H 4.25 . . 100 . 20 LYS HB2 H 1.75 . . 101 . 20 LYS HG2 H 1.36 . . 102 . 20 LYS HD2 H 1.71 . . 103 . 20 LYS HE2 H 2.93 . . 104 . 21 VAL H H 8.34 . . 105 . 21 VAL HA H 4.05 . . 106 . 21 VAL HB H 2.02 . . 107 . 21 VAL HG1 H 0.9 . . 108 . 22 GLN H H 8.10 . . 109 . 22 GLN HA H 4.11 . . 110 . 22 GLN HB2 H 2.03 . . 111 . 22 GLN HG2 H 2.24 . . stop_ save_